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Effect of Ovarian Extract on Oocyte Maturation and Early Embryonic Development in Pigs
Seul-Gi Yang(Seul-Gi Yang),Jae-Hun Choi(Jae-Hun Choi),Young-Seo Jo(Young-Seo Jo),Ye-Won Kim(Ye-Won Kim),Dong-Mok Lee(Dong-Mok Lee),Hyo-Jin Park(Hyo-Jin Park),Deog-Bon Koo(Deog-Bon Koo) 한국동물보건학회 2022 한국동물보건학회지 Vol.1 No.2
Various factors in the ovary are known to regulate oocyte maturation and hormone secretory functions; however, the effect of ovarian extract (OE) on oocyte maturation and embryonic development in pigs remains unknown. In this study, we first evaluated whether OE supplementation in the in vitro maturation (IVM) medium alters the oocyte maturation capacity by affecting glucose/amino acid metabolites, meiotic maturation, cumulus cell (CC) expansion, and antioxidants. Various OE concentrations (50, 100, 200, 500, and 5000 μg/mL) were included in the IVM medium. Only the oocytes treated with 100 μg/mL OE exhibited an improved meiotic maturation rate when compared with that of the other groups (non-treated group, 78.6 ± 3.0% vs. 100 μg/mL OE-treated group, 81.6 ± 4.3%); however, the difference was not significant. To observe the changes in glucose and amino acid metabolism in the OE-treated oocytes, we measured the amounts of diverse constituents (glucose, lactate, glutamine, and ammonia) in the IVM medium containing OE. Lactate and ammonia levels in the OE-treated group after 44 h of IVM were higher (p < 0.01) than those in the non-treated group. In addition, the expression of the CC expansion factors (Has2 and Tnfaip6) significantly increased (p < 0.05), whereas the mRNA expression levels of antioxidative enzymes (Sod1, Cat, and Gpx1) significantly diminished (p < 0.05) in the OE-treated group. Moreover, mature oocytes treated with 100 μg/mL OE demonstrated increased subsequent embryonic development rates after 144 h of IVM. Thus, the addition of OE in IVM mediums may improve oocyte maturation capacity which could enhance antioxidative enzyme activation, energy metabolism, and expression of the CC expansion factors in porcine oocytes.
Seul Gi Baek(Seul Gi Baek),Jin Ju Park(Jin Ju Park),Sosoo Kim(Sosoo Kim),Mi-Jeong Lee(Mi-Jeong Lee),Ji-Seon Paek(Ji-Seon Paek),Jangnam Choi(Jangnam Choi),Ja Yeong Jang(Ja Yeong Jang),Jeomsoon Kim(Jeom 한국식물병리학회 2022 Plant Pathology Journal Vol.38 No.6
Fusarium head blight (FHB) is one of the most serious diseases in barley and wheat, as it is usually accompanied by the production of harmful mycotoxins in the grains. To identify FHB-resistant breeding resources, we evaluated 60 elite germplasm accessions of barley (24) and wheat (36) for FHB and mycotoxin accumulation. Assessments were performed in a greenhouse and five heads per accession were inoculated with both Fusarium asiaticum (Fa73, nivalenol producer) and F. graminearum (Fg39, deoxynivalenol producer) strains. While the accessions varied in disease severity and mycotoxin production, four wheat and one barley showed <20% FHB severity repeatedly by both strains. Mycotoxin levels in these accessions ranged up to 3.9 mg/ kg. FHB severity was generally higher in barley than in wheat, and Fa73 was more aggressive in both crops than Fg39. Fg39 itself, however, was more aggressive toward wheat and produced more mycotoxin in wheat than in barley. FHB severity by Fa73 and Fg39 were moderately correlated in both crops (r = 0.57/0.60 in barley and 0.42/0.58 in wheat). FHB severity and toxin production were also correlated in both crops, with a stronger correlation for Fa73 (r = 0.42/0.82 in barley, 0.70 in wheat) than for Fg39.
( Seul-gi Choi ),( Xiongzhe Han ) 한국농업기계학회 2023 한국농업기계학회 학술발표논문집 Vol.28 No.2
Amid the rapid evolution of research in artificial intelligence, autonomous robotics, especially in the realm of self-driving robots, is gaining significant attention. Simultaneous Localization and Mapping (SLAM) technology enables mobile robots to comprehend their surroundings and navigate autonomously. This paper focuses on autonomous navigation and obstacle avoidance research in orchard environments using the mobile robot platform Scout 2.0, which relies on the primary sensor, LIDAR. In brief, the research entails introducing the Scout 2.0 robot platform and establishing its hardware infrastructure, capturing and storing environmental data using the gmapping algorithm, leveraging this data to understand the terrain and achieve precise robot localization, employing the A* algorithm to generate robot paths while considering complex environments, and continually monitoring sensor data for safe trajectory adjustments during autonomous navigation. This study summarizes the critical aspects of research, from map creation with gmapping to secure and efficient autonomous navigation within challenging orchard terrain, ultimately contributing to improved efficiency and productivity in agricultural operations.
인지행동 음악치료가 음악전공자들의 무대공포증에 미치는 영향
최슬기 ( Seul Gi Choi ),강경선 ( Kyung Sun Kang ) 연세대학교 음악연구소 2018 음악이론포럼 Vol.25 No.2
본 연구는 인지행동 음악치료 프로그램이 음악전공자들의 무대공포증에 미치는 영향을 알아보고자 실시되었다. 연구대상은 서울 소재의 음악대학 및 예술대학 3곳에서 무대공포증을 겪는 음악전공자를 대상으로 무대불안 척도를 사용하여 높은 점수를 얻은 음악전공자 10명을 선정하였다. 실험집단 5명과 통제집단 5명으로 구분하여 무선 배치하였으나, 실험집단 1명이 중도 포기를 하여 실험집단 4명, 통제집단 5명으로 프로그램이 진행되었다. 본 프로그램은 2018년 3월 15일부터 4월 17일까지 주 2회 50분으로 10회기로 구성하여 진행되었다. 연구결과 무대불안 척도점수에서 실험집단의 평균이 통제집단보다 37점 낮게 나타나 음악치료가 효과적인 중재임이 입증되었다. 양적연구와 함께 무대공포증을 겪는 음악전공자의 질적인 변화를 알아보기 위하여 참여자들이 작성한 설문지와 연구자의 관찰기록내용, 참여자들의 경험 및 변화에 대한 인터뷰를 실시하여 분석하였다. 내용분석의 결과, 참여자들의 무대공포에 대한 경험변화는 원인 찾기, 생각 공유, 나의 변화, 새로운 음악경험, 스트레스 해소, 새로운 다짐으로 6가지의 구체적인 하위영역이 도출되었다. 이러한 질적연구의 결과에서도 음악치료는 내담자들에게 의미 있는 치료적 경험으로 나타났음을 알 수 있다. The purpose of this study was to investigate the effects of Cognitive-Behavior Music Therapy on stage fright of students who are majoring music. The subjects of this study were 10 musicians majoring in stage phobia at the music college in Seoul. They were randomly assigned into an experimental group and a control group. Cognitive-Behavior Music Therapy was conducted total of 10 sessions from March 15 to April 17, 2018, with a 50 minute session twice a week. Stage Fright scores were measured before and after the program in order to examine the effect of Cognitive-Behavior Music Therapy. As a result, the mean score of the experimental group was lower than the control group by 37 points in the score of Stage Anxiety Scale, and music therapy proved to be an effective intervention. In order to investigate the qualitative changes of musical majors who are experiencing stage phobia with quantitative research, questionnaires made by the participants, interview records of the researchers, interviews about the experiences and changes of the participants were analyzed. And as a result of qualitative research, regarding change of the participants’ experience toward Stage Fright, six (6) sub-factors were drawn which include ‘Finding the cause’, ‘sharing thoughts’, ‘my change‘, ‘new music experience’, ‘relieving stress’, ‘new compromise’. As a result of these qualitative studies, it can be seen that music therapy is a meaningful therapeutic experience for clients.