Platelet Activation: The Mechanisms and Potential Biomarkers

Yun, Seong-Hoon,Sim, Eun-Hye,Goh, Ri-Young,Park, Joo-In,Han, Jin-Yeong Hindawi Publishing Corporation 2016 BioMed research international Vol.2016 No.-

<P>Beyond hemostasis and thrombosis, an increasing number of studies indicate that platelets play an integral role in intercellular communication, mediating inflammatory and immunomodulatory activities. Our knowledge about how platelets modulate inflammatory and immunity has greatly improved in recent years. In this review, we discuss recent advances in the pathways of platelet activation and potential application of platelet activation biomarkers to diagnosis and prediction of disease states.</P>

Genetic relationship and cytochemical analysis of Arabidopsis thaliana mutants induced by gamma irradiation

Eun Jeong Goh,Jin Baek Kim,Bo Keun Ha,Sang Hoon Kim,Si Yong Kang,Cheol Seong Jang,Sun Goo Hwang,Dong Sub Kim 한국육종학회 2011 한국육종학회 학술발표회 발표요지 Vol.43 No.-

Skin Safety Evaluation of Long-Term treatment of an Electrolyzed Neutral Oxidizing Water on C57BL/6 mice

Md. Habibur Rahman,Ailyn Fadriquela,Johny Bajgai,Seong-Hoon Goh,Cheol-Su Kim,Kyu-Jae Lee 한국물학회 2020 한국물학회지 Vol.8 No.1

Skin is a complex barrier organ that can fight against various endogenous and exogenous factors. The damaged skin barrier can lead to different skin problems that can exhibit the skin condition such as a decrease of elasticity and sebum level, an increase of water loss, strengthening of keratinocyte cohesion and wrinkle formation, which will eventually lead to different skin diseases. With the current use of chlorine-rich water as disinfectant and its potential in medical field, it is important to check its safety use. The objective of the study was to evaluate skin safety of electrolyzed neutral oxidizing water (ENOW) (pH: 7.11, oxidation reduction potential: 450 mV, available chlorine concentration: 5.0 ppm) after the long-term use (90-day skin application) on the skin of C57BL/6 mice compared to normal control group (NC) treated with tap water (TW) (pH: 7.8, oxidation reduction potential: 500mV, available chlorine concentration: 0.7 ppm). We were measured skin state such as the moisture, elasticity, sebum, pore size, and wrinkle. Oxidative stress markers such as reactive oxygen species (ROS), nitric oxide (NO), glutathione peroxides (GPx) and catalase (CAT) were measuredin using serum and skin lysate samples. Inflammation cytokines such as IL-1α, IL-1β, IL-13, GM-CSF and TNF-α, and the Th1/Th2 cytokines such as IL-2, IL-4, IL-5, IL-6, IL-10 and IL-12 were also analyzed. In consequently, The skin state of ENOW group showed higher level of elasticity and moisture compared to NC group. ROS and NO levels in skin did not show adverse effect in ENOW group. GPx were also controlled in all groups, however, a significant decrease in CAT level was observed in both NC and ENOW groups. Moreover, serum inflammatory cytokine of ENOW group did not show significant difference as compared to NC and TW groups, however, GM-CSF showed a significantly lower level in the ENOW group than NC group. Cytokines related to allergic response showed no difference in the skin. Taken together, long term treatment of ENOW might be safe on skin in vivo . However, more clinical studies are needed to verify the skin safety of ENOW.

블루투스를 이용한 분실방지 시스템

임수현(Soo-Hyeon Lim),김기훈(Ki-Hoon Kim),김형섭(Hyung-Seok Kim),유동진(Dong-Jin Yoo),강성혁(Seong-Hyeok Kang),김상진(Sang-Jin Kim),고영진(Young-Jin Goh) 대한전기학회 2016 대한전기학회 학술대회 논문집 Vol.2016 No.11

증례 : 항응고요법 중 재발한 신경색증을 동반한 원발성 항인지질증후군 1예

김민희 ( Min Hee Kim ),고충원 ( Choong Won Goh ),김상현 ( Sang Hyun Kim ),한성훈 ( Seong Hoon Han ),장기준 ( Ki Jun Chang ),황진태 ( Jin Tae Hwang ),유성인 ( Sung In Yu ) 대한내과학회 2013 대한내과학회지 Vol.84 No.2

원발성 항인지질증후군은 주로 정맥 혈전증으로 나타나게 되며 동맥 혈전증과 관련된 사례는 드물다. 저자들은 국내에 보고된 바 없는 항응고요법 중 재발한 신경색증으로 진단된 원발성 항인지질증후군을 경험하였다. 이에 문헌고찰과 함께 보고하는 바이다. Anti-phospholipid syndrome is characterized by the occurrence of venous or arterial thrombosis in the presence of anti-phospholipid antibodies and is associated with morbidity during pregnancy. Arterial thromboses are less common than venous thromboses and most frequently manifest with features consistent with ischemia or infarction. Only a few cases of arterial thrombosis with primary anti-phospholipid syndrome have been reported in Korea. We report a 41-year-old man with anti-phospholipid syndrome who had abdominal pain due to a recurrent renal infarction, and we review the literature on anti-phospholipid syndrome. (Korean J Med 2013;84:269-273)

한국 미세먼지 계절관리제와 중국 추동절기 대기오염방지 대책 비교 분석

김민주(Min-joo Kim),고득훈(Deuk-hoon Goh),이감성(Gam-seong Lee),권도은(Do-eun Kwon),박세원(Se-won Park) 한국에너지기후변화학회 2023 한국에너지기후변화학회 학술대회 Vol.2023 No.5

Redox Effects of Molecular Hydrogen in C57BL/6 Mouse Model with Trimethyltin-induced Cognitive Dysfunction

Johny Bajgai,Md. Habibur Rahman,Ailyn Fadriquela,Subham Sharma,Dong-Heui Kim,Seong-Hoon Goh,In-Soo You,Yun-Su Na,Nam-Kyu Kong,Soo-Ki Kim,Cheol-Su Kim,Kyu-Jae Lee 한국물학회 2020 한국물학회지 Vol.8 No.1

Various neurodegenerative diseases including dementia and cognitive dysfunction pathogenesis are caused due to oxidative stress. Several studies have proved that molecular hydrogen (H2) acts as a therapeutic and preventive anti-oxidant, however, H2 effect on dementia is poorly documented. In the present study, we investigated the effects of H2 inhalation on trimethyltin (TMT)-induced memory dysfunction in the C57BL/6 mice. For this, mice were intraperitoneally injected a single dosage of TMT 2.6 mg/kg body weight. The subjects were randomly divided into the following groups: normal control without TMT induction, TMT only as a negative control (TMT injection + saline administration), positive control group [TMT injection + lithium chloride (50 mg/kg body weight) injection], H2-2wk group [TMT injection + H2 gas inhalation (once a day for 30 min for 2 weeks) and H2-4wk group [TMT injection + H2 gas inhalation (once a day for 30 min for 4 weeks). As a result, TMT-exposed mice showed symptoms such as tremor, seizure and aggressive behavior. Meanwhile, H2-treated groups after TMT treatment showed significantly increased spatial cognition abilities in the Y-maze test. Likewise, the level of the oxidative stress markers such as ROS, NO and MDA were found to be decreased in H2-2wk and H2-4wk groups as compared to the TMT only group in both serum and brain lysate, coherently, antioxidant enzymatic activities were increased in H2-2wk and H2-4wk groups compared to TMT only group. Moreover, H2 showed prevention effect of neuronal apoptosis through the suppression of apoptotic signaling molecules such as BAX, and cleaved caspase-7. Overall, H2 inhalation exhibited memory enhancing activity and decreased the oxidative stress markers and suppressed apoptotic signaling molecules on TMT-inducd mice. Therefore, H2 might be one of candidates for repairing dementia and neuro-degenerative diseases which shows symptoms such as dysfunction of learning, memory and cognition. However further studies are needed to clarify this notion.

The Effect of a Granule-type Anti-hangover Compound, Quechung, on Acute Alcohol-induced Hangover in Healthy Subjects : a Randomized Crossover Study

Subham Sharma,Johny Bajgai,Ailyn Fadriquela,Md. Habibur Rahman,Trinh Thi Thuy,Seong-Hoon Goh,Cheol-Su Kim,Kijong Yu,Kyu-Jae Lee 한국물학회 2020 한국물학회지 Vol.8 No.1

About two billion people in the world drink alcoholic products and among them, more than 76 million are suffering from serious alcoholic disorder including hangover. Hanover can be caused by the by-products which are produced in the process of alcohol metabolism such as acetaldehyde and acetate in human body. There are various type of anti-hangover-related commercial products which can help to somewhat reduce hangover symptoms. In this study, we investigated the effects of Quechung which is a granule-type of anti-hangover compounds containing functional substances such as HK mycelial culture of Lentinus edodes, Hovenia dulcis fruit extract, Lactobicillus fermentum MG590 and so on. The subjects were 20 healthy men and women, and washout period for the crossover design was 7 days. Participants were randomly divided into two groups: the control group (alcohol + purified water) and the experimental group (alcohol + Quechung). The experimental group was first supplied with the anti-hangover compound before and after 30 min of 20% alcohol intake (7.8 g/kg body weight). Blood pressure, temperature, heart rate, and alcohol concentration in breath and blood were measured one time before alcohol intake and also 3 times after Quechung intake at an interval of 1 h. Biochemical parameters such as albumin, electrolytes, lipid profile, liver function markers, alcohol level in breath and blood, acetaldehyde concentration were analyzed. Consequently, breath alcolol concentration was lower in the experimental group than control group after 1 h, especially showed statistical difference in female. Blood alcohol concentration before and after treatment also showed significant decrease in experimental group, whereas blood acetaldehyde showed significant increase in control group. Especially female participants showed higher effect than male. In the reslut of AST, experimental group showed little difference before and after treatment but control group showed significant increase, in addition female much higher effect than male. Taken together, this anti-hangover compound, Quechung, clinically showed the hangover-relieving effect on the acute alcohol-induced hangover symptom based on the positive results of alcohol concentration, acetaldehyde concentration, and level of liver function markers etc. in addition, Quechung also can influence on the protection of liver and blood vessel. This clinical study has a significance in that it provides scientific evidence to systemically establish database for the effectiveness and efficiency of the commercial anti-hangover products, and to help consummer’s easy choice and their better health.

Application of Hydrogen for the Health Improvement in Vietnam

Trinh Thi Thuy,Johny Bajgai,Md. Habibur Rahman,Ailyn Fadriquela,Subham Sharma,Goh Seong Hoon,Cheol-Su Kim,Kyu-Jae Lee 한국물학회 2020 한국물학회지 Vol.8 No.1

Health and vibrant life are required by everyone around the globe. To improve quality of life, maintaining a healthy state, and to prevent various diseases, and to evaluate the effects of potentially improve quality of life increasing factors are important. In recent years, oxidative stress has been postulated to be an important factor in the pathogenesis and development of lifestyle-related diseases, cancer, and the aging process. Despite the clinical importance of oxidative damage due to oxidative stress, antioxidants have been of limited therapeutic success. Of these, molecular hydrogen (H2) is known as a “novel” antioxidant in preventive and therapeutic applications. H2 shows not only effects against oxidative stress but also various anti-inflammatory and anti-allergic effects. Thus, H2 is nowadays used in daily life as well as and in medical field in both developed and developing countries such as Japan, USA, and China. Unfortunately, in Vietnam, one of the developing countries in the world has a poor health system and a high death rate due to non-communicable diseases like cancer, cardiovascular, and diabetes. Growing evidence has shown that hydrogen gas can either alleviate the side effects caused by conventional chemotherapeutics, or suppress the growth of cancer cells and xenograft tumor, suggesting its broad potent application in clinical therapy. Besides that studies have reported that H2 also has effects of on diseases such as diabetes, cerebral infarction, and Alzheimer’s disease. However in Vietnam, currently there are only a few research related to H2 has conducted that mainly focus on the application in the environment, energy industry. Therefore, H2 in Vietnam in medical field needs to be exploited in the future as it is completely new area.

Safety Evaluation of Drinking Mineral Supplement Hypochlorous Acid Water on Helicobacter pylori-Infected C57BL/6 Mouse Model

Md. Habibur Rahman,Johny Bajgai,Ailyn Fadriquela,Subham Sharma,Trinh Thi Thuy,Seong-Hoon Goh,Cheol-Su Kim,Kyu-Jae Lee 한국물학회 2020 한국물학회지 Vol.8 No.1

Hypochlorous acid water can promote pruritus as an adverse effect under certain conditions, which should be taken into account to ensure safety. In recent days, Mineral Supplement Hypochlorous Acid Water (MS-HOCl) has received massive attention in research as a new-concept cleansing agent due to its high sterilizing properties, easy accessibility, and safety aspects. This present study was conducted to evaluate the drinking effects of MS-HOCl treatment of the Helicobacter pylori (H. pylori )-infected C57BL/6 mouse model to verify the safety effects. The experimental mice were divided into 3 groups: NC (Purified water + no infection), PW (Purified water + H. pylori infection), and MS-HOCL (MS-HOCl + H. pylori infection). Water was supplied through water bottle for mouse for 4 weeks, and changed once a day. We measured the bodyweight, and total WBC and its differential counts such as lymphocytes, monocytes, neutrophil, eosinophil and basophil. Besides that, we also tested biochemical markers such as reactive oxygen species (ROS), nitric oxide (NO), glutathione peroxides (GPx), and calcium activity in both serum and stomach lysate samples. Our results showed that daily oral administration of MS-HOCl water for 4 wk did not show any abnormal findings about hematological and biochemical parameters such as ROS, NO, GPx, and calcium activity in both serum and stomach lysate. There were no significant differences in bodyweight between the three groups. Overall, our results showed no negative effects of drinking MS-HOCl in the H. pylori -infected C57BL/6 mice model. However, more controlled studies are needed to determine the safety of drinking MS-HOCl.