The Effect of a Granule-type Anti-hangover Compound, Quechung, on Acute Alcohol-induced Hangover in Healthy Subjects : a Randomized Crossover Study

Subham Sharma,Johny Bajgai,Ailyn Fadriquela,Md. Habibur Rahman,Trinh Thi Thuy,Seong-Hoon Goh,Cheol-Su Kim,Kijong Yu,Kyu-Jae Lee 한국물학회 2020 한국물학회지 Vol.8 No.1

About two billion people in the world drink alcoholic products and among them, more than 76 million are suffering from serious alcoholic disorder including hangover. Hanover can be caused by the by-products which are produced in the process of alcohol metabolism such as acetaldehyde and acetate in human body. There are various type of anti-hangover-related commercial products which can help to somewhat reduce hangover symptoms. In this study, we investigated the effects of Quechung which is a granule-type of anti-hangover compounds containing functional substances such as HK mycelial culture of Lentinus edodes, Hovenia dulcis fruit extract, Lactobicillus fermentum MG590 and so on. The subjects were 20 healthy men and women, and washout period for the crossover design was 7 days. Participants were randomly divided into two groups: the control group (alcohol + purified water) and the experimental group (alcohol + Quechung). The experimental group was first supplied with the anti-hangover compound before and after 30 min of 20% alcohol intake (7.8 g/kg body weight). Blood pressure, temperature, heart rate, and alcohol concentration in breath and blood were measured one time before alcohol intake and also 3 times after Quechung intake at an interval of 1 h. Biochemical parameters such as albumin, electrolytes, lipid profile, liver function markers, alcohol level in breath and blood, acetaldehyde concentration were analyzed. Consequently, breath alcolol concentration was lower in the experimental group than control group after 1 h, especially showed statistical difference in female. Blood alcohol concentration before and after treatment also showed significant decrease in experimental group, whereas blood acetaldehyde showed significant increase in control group. Especially female participants showed higher effect than male. In the reslut of AST, experimental group showed little difference before and after treatment but control group showed significant increase, in addition female much higher effect than male. Taken together, this anti-hangover compound, Quechung, clinically showed the hangover-relieving effect on the acute alcohol-induced hangover symptom based on the positive results of alcohol concentration, acetaldehyde concentration, and level of liver function markers etc. in addition, Quechung also can influence on the protection of liver and blood vessel. This clinical study has a significance in that it provides scientific evidence to systemically establish database for the effectiveness and efficiency of the commercial anti-hangover products, and to help consummer’s easy choice and their better health.

Customer satisfaction of feminine cleanser containing human stem cell conditioned media

Vira Kchorng,Subham Sharma,Johny Bajgai,Ailyn Fadriquela,Md. Habibur Rahman,Cheol-Su Kim,Kyu-Jae Lee 한국물학회 2021 한국물학회지 Vol.9 No.1

Feminine cleanser is widely used for feminine hygiene because the vulva and vaginal surface are the most sensitive and fragile area. In particular, decreased estrogen level during menopause can cause genital symptoms (e.g., dryness, burning and irritation), sexual symptoms (e.g., lack of lubrication, pain, and impaired function) and urinary symptoms (e.g., urinary incontinence and urinary tract infection). Recently, commercial feminine cleanser shows the enhanced functions related with feminine health such as moisturizing, elasticity and anti-bacterial effect as well as cleaning. The aim of this study was to investigate the effect of the feminine cleanser (CellZyme Secret Therapy Plus, Pharmacal International Co. Ltd., Seoul, Korea) on various female-related symptoms in the women who have used this cleanser for over 1 month. The feminine cleanser used in this study was jelly-type formulation and it contains functional substances including human mesenchymal stem cell conditioned media (150,000ppm), peptides and various natural plant extract. The questionaire items were odor, leukorrhea, itching, dryness, vaginal contractile force, urinary incontinence and menstrual cramps. We divided into 5 grades according to the improvement degree of symptom: none (1), a little (2), moderate (3), good (4), and very good (5). Data was analyzed by using the IBM® SPSS® statistics tool. A total of 46 women responded on the survey, among them 28.26% was involved in the menopausal stage and 84% responded to be satisfied with the effect of the cleanser. According to the symptoms, respondents replied to have experienced positive effect (2 - 5 grades) as follows: foul smell 87%, leukorrhea 90%, and itching 96%, vaginal dryness 87%, vaginal contractile force 83%, urinary incontinence 61% and menstruation cramp (58%). In conclusion, the use of feminine cleanser is expected to useful for feminine hygiene and women's health through improvement of vulvar and vaginal environment such as pH and microflora.

Safety Evaluation of Drinking Mineral Supplement Hypochlorous Acid Water on Helicobacter pylori-Infected C57BL/6 Mouse Model

Md. Habibur Rahman,Johny Bajgai,Ailyn Fadriquela,Subham Sharma,Trinh Thi Thuy,Seong-Hoon Goh,Cheol-Su Kim,Kyu-Jae Lee 한국물학회 2020 한국물학회지 Vol.8 No.1

Hypochlorous acid water can promote pruritus as an adverse effect under certain conditions, which should be taken into account to ensure safety. In recent days, Mineral Supplement Hypochlorous Acid Water (MS-HOCl) has received massive attention in research as a new-concept cleansing agent due to its high sterilizing properties, easy accessibility, and safety aspects. This present study was conducted to evaluate the drinking effects of MS-HOCl treatment of the Helicobacter pylori (H. pylori )-infected C57BL/6 mouse model to verify the safety effects. The experimental mice were divided into 3 groups: NC (Purified water + no infection), PW (Purified water + H. pylori infection), and MS-HOCL (MS-HOCl + H. pylori infection). Water was supplied through water bottle for mouse for 4 weeks, and changed once a day. We measured the bodyweight, and total WBC and its differential counts such as lymphocytes, monocytes, neutrophil, eosinophil and basophil. Besides that, we also tested biochemical markers such as reactive oxygen species (ROS), nitric oxide (NO), glutathione peroxides (GPx), and calcium activity in both serum and stomach lysate samples. Our results showed that daily oral administration of MS-HOCl water for 4 wk did not show any abnormal findings about hematological and biochemical parameters such as ROS, NO, GPx, and calcium activity in both serum and stomach lysate. There were no significant differences in bodyweight between the three groups. Overall, our results showed no negative effects of drinking MS-HOCl in the H. pylori -infected C57BL/6 mice model. However, more controlled studies are needed to determine the safety of drinking MS-HOCl.

Redox Effects of Molecular Hydrogen in C57BL/6 Mouse Model with Trimethyltin-induced Cognitive Dysfunction

Johny Bajgai,Md. Habibur Rahman,Ailyn Fadriquela,Subham Sharma,Dong-Heui Kim,Seong-Hoon Goh,In-Soo You,Yun-Su Na,Nam-Kyu Kong,Soo-Ki Kim,Cheol-Su Kim,Kyu-Jae Lee 한국물학회 2020 한국물학회지 Vol.8 No.1

Various neurodegenerative diseases including dementia and cognitive dysfunction pathogenesis are caused due to oxidative stress. Several studies have proved that molecular hydrogen (H2) acts as a therapeutic and preventive anti-oxidant, however, H2 effect on dementia is poorly documented. In the present study, we investigated the effects of H2 inhalation on trimethyltin (TMT)-induced memory dysfunction in the C57BL/6 mice. For this, mice were intraperitoneally injected a single dosage of TMT 2.6 mg/kg body weight. The subjects were randomly divided into the following groups: normal control without TMT induction, TMT only as a negative control (TMT injection + saline administration), positive control group [TMT injection + lithium chloride (50 mg/kg body weight) injection], H2-2wk group [TMT injection + H2 gas inhalation (once a day for 30 min for 2 weeks) and H2-4wk group [TMT injection + H2 gas inhalation (once a day for 30 min for 4 weeks). As a result, TMT-exposed mice showed symptoms such as tremor, seizure and aggressive behavior. Meanwhile, H2-treated groups after TMT treatment showed significantly increased spatial cognition abilities in the Y-maze test. Likewise, the level of the oxidative stress markers such as ROS, NO and MDA were found to be decreased in H2-2wk and H2-4wk groups as compared to the TMT only group in both serum and brain lysate, coherently, antioxidant enzymatic activities were increased in H2-2wk and H2-4wk groups compared to TMT only group. Moreover, H2 showed prevention effect of neuronal apoptosis through the suppression of apoptotic signaling molecules such as BAX, and cleaved caspase-7. Overall, H2 inhalation exhibited memory enhancing activity and decreased the oxidative stress markers and suppressed apoptotic signaling molecules on TMT-inducd mice. Therefore, H2 might be one of candidates for repairing dementia and neuro-degenerative diseases which shows symptoms such as dysfunction of learning, memory and cognition. However further studies are needed to clarify this notion.

차아염소산수 공중 분무를 통한 인체 병원성 황색포도상구균 살균효과 평가

Md. Habibur Rahman,Johny Bajgai,Ailyn Fadriquela,Subham Sharma,Trinh Thi Thuy,Goh Seong Hoon,Cheol-Su Kim,Kyu-Jae Lee 한국물학회 2020 한국물학회지 Vol.8 No.1

Hypochlorous acid has very ideal condition such as powerful and broad sterilizing effect, easy accessibility, low price and high safety as a disinfectant. It can be applied by various ways, but the effect may vary depending on the method, so it is necessary to establish use standard for the maximized effect. In this study, we investigated the sterilizing effect of hypochlorous acid indoor environments contaminated with Staphylococcus aureus using fogging method. Hypochlorous acid water was classified two: Huureka-E generated by electrolysis [pH 5.4, oxidation-reduction potential (ORP) 530, active chlorine concentration (ACC) 51], and Huureka-M produced by a mixing and dilution method (pH 5.5, ORP 913, ACC 53). Blood agar plate were put on the table 1 m high in an enclosed room, then hypochlorous acid was fogged up to 80% humidity, and S. aureus-diluted solution was sprayed three times on the air. The plate was moved for bacterial culture 0 min 30 min, 60 min, and 90 min after spraying bacteria. As a result of the bacterial culture, the growth inhibitory rate of Huureka-M was 92.2 ± 0.8%, 96.0 ± 0.6% and 97.4 ± 0.1% time-dependently (p < 0.001 vs. 0 minm, respectively), and that of Huureka-E was 66.1 ± 1.3%, 76.4 ± 2.4% and 98.2 ± 0.6% (p < 0.001 vs. 0 min, respectively). In confluence, the fogging of both electrolysis-type and mixing-type hypochlorous acid water showed highly satisfactory sterilizing power as a disinfectant. Since spraying or fogging is a highly effective application method for a large space, hypochlorous acid can be recommended to be utilized in the hospital, agriculture sector, livestock industry, public facilities, etc. Further study will be necessary to be standardized the sterilizing protocol according to the application method and ACC.

Anti-bacterial and anti-fungal effects of feminine cleanser containing human stem cell conditioned media

Vira Kchorng,Md. Habibur Rahman,Johny Bajgai,Ailyn Fadriquela,Subham Sharma,Cheol-Su Kim,Kyu-Jae Lee 한국물학회 2021 한국물학회지 Vol.9 No.1

Vulvar area is susceptible to bacterial and fungal infection especially due to low pH environment. Thus, disruption of pH on skin (pH 5.5) and vagina (pH 3.5-4.5) can affect normal vaginal flora such as lactobasilli and lead to genitourinary infection by the growth of harmful bacteria. Currently the feminine cleanser is required to have further functions such as protection and improvement of a specific woman's diseases beside cleaning. The feminine cleanser (CellZyme Secret Therapy Plus, Pharmacal International Co. Ltd., Seoul, Korea) used in this study contains the functional substances such as human stem cell-conditioned media and various kinds of plants extract. we conducted this experiment to investigate the effects of the feminine cleanser on pathogenic bacteria and fungus. Three bacterial strains (Pseudomonas aeruginosa, Escherichia coli and Staphylococcus aureus), and one fungus (Candida albicans) were cultured in the blood agar, treated with a feminine cleanser, and incubated for 24 h at 37℃. Results demonstrated that a time-dependent treatment with a feminine cleanser markedly suppressed bacterial and fungal growth. In conclusion, the feminine cleanser containing stem cell-conditioned media and plants extract (CellZyme Secret Therapy Plus) showed anti-bacterial and anti-fungal effect, which is expected to help protection of genitourinay infection.

Application of Hydrogen for the Health Improvement in Vietnam

Trinh Thi Thuy,Johny Bajgai,Md. Habibur Rahman,Ailyn Fadriquela,Subham Sharma,Goh Seong Hoon,Cheol-Su Kim,Kyu-Jae Lee 한국물학회 2020 한국물학회지 Vol.8 No.1

Health and vibrant life are required by everyone around the globe. To improve quality of life, maintaining a healthy state, and to prevent various diseases, and to evaluate the effects of potentially improve quality of life increasing factors are important. In recent years, oxidative stress has been postulated to be an important factor in the pathogenesis and development of lifestyle-related diseases, cancer, and the aging process. Despite the clinical importance of oxidative damage due to oxidative stress, antioxidants have been of limited therapeutic success. Of these, molecular hydrogen (H2) is known as a “novel” antioxidant in preventive and therapeutic applications. H2 shows not only effects against oxidative stress but also various anti-inflammatory and anti-allergic effects. Thus, H2 is nowadays used in daily life as well as and in medical field in both developed and developing countries such as Japan, USA, and China. Unfortunately, in Vietnam, one of the developing countries in the world has a poor health system and a high death rate due to non-communicable diseases like cancer, cardiovascular, and diabetes. Growing evidence has shown that hydrogen gas can either alleviate the side effects caused by conventional chemotherapeutics, or suppress the growth of cancer cells and xenograft tumor, suggesting its broad potent application in clinical therapy. Besides that studies have reported that H2 also has effects of on diseases such as diabetes, cerebral infarction, and Alzheimer’s disease. However in Vietnam, currently there are only a few research related to H2 has conducted that mainly focus on the application in the environment, energy industry. Therefore, H2 in Vietnam in medical field needs to be exploited in the future as it is completely new area.