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( Semi Jeon ),( Nara Lim ),( Sanghee Park ),( Misun Park ),( Seonghan Kim ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.2
Two molecular epidemiologic methods, IS6110 restriction fragment length polymorphism (IS6110-RFLP) and 24-locus mycobacterial interspersed repetitive unit-variable-number tandem repeat (MIRU-VNTR), are used worldwide in studies of Mycobacterium tuberculosis (MTB). Conversely, because of its poor resolution, pulsed-field gel electrophoresis (PFGE) is not widely used for MTB. In this study, we improved the 24-locus MIRU-VNTR and PFGE protocols and compared the effectiveness of these approaches for the molecular typing of MTB using 75 clinical isolates obtained from a cohort investigation of high-risk populations infected with MTB. The 24-locus MIRU-VNTR method demonstrated superior discriminatory ability, followed by PFGE and IS6110-RFLP. Next, we analyzed six isolates with clear epidemiologic connections; that is, isolates from patients who attended the same school. IS6110-RFLP and PFGE identified these samples as the same type. By contrast, according to MIRU-VNTR, two isolates differed from four other isolates at one locus each; one isolate was identified as Mtub29 and the other as QUB-26. In summary, the 24-locus MIRU-VNTR assay was the most useful molecular typing method among the three methods investigated due to its discriminatory power, short time required, and availability as an epidemiologic investigation tool. PFGE was the second-best method. Compared with the other loci assessed in the 24-locus MIRU-VNTR assay, the Mtub29 and QUB-26 loci appeared to exhibit greater variability during transmission.
Jeon, Young-Chul,Park, Bitna,Park, Semi,Kim, Sung-Hoon American Scientific Publishers 2014 Journal of nanoscience and nanotechnology Vol.14 No.12
<P>Carbon coils could be synthesized using C2H2/H2 as source gases along with SF6 as an incorporated additive gas using a thermal chemical vapor deposition (CVD) system. To obtain geometrically controlled carbon coils, a cyclic process, namely the turning on and off of C2H2 or SF6 flow during the initial reaction stage, was carried out. According to the different reaction processes, different interruption/injection times of C2H2 or SF6 flow and different injection sequences of the gas flow were investigated while maintaining the identical overall injection time of C2H2 and/or SF6 flow. The formation of carbon microcoils (CMCs) is favored by the lowest interruption/injection time ratio of SF6 flow within one cycle. In addition, the injection of SF6 flow prior to the injection of C2H2 flow promotes the formation of CMCs. Based on these results we revealed the role of the SF6 flow injection for the enhanced formation of geometrically controlled CMCs. The etching of materials, thereby promoting an increase in the number of nucleation sites for the survived growth species to form CMCs, by the increased fluorine concentration, originating from the dominant SF6 influx, is understood to be the main cause for the exclusive CMCs formation.</P>
Recent Advances in Feature Detectors and Descriptors
Haeseong Lee,Semi Jeon,Inhye Yoon,Joonki Paik 대한전자공학회 2016 IEIE Transactions on Smart Processing & Computing Vol.5 No.3
Local feature extraction methods for images and videos are widely applied in the fields of image understanding and computer vision. However, robust features are detected differently when using the latest feature detectors and descriptors because of diverse image environments. This paper analyzes various feature extraction methods by summarizing algorithms, specifying properties, and comparing performance. We analyze eight feature extraction methods. The performance of feature extraction in various image environments is compared and evaluated. As a result, the feature detectors and descriptors can be used adaptively for image sequences captured under various image environments. Also, the evaluation of feature detectors and descriptors can be applied to driving assistance systems, closed circuit televisions (CCTVs), robot vision, etc.
Kim, Junyoung,Jeon, Semi,Rhie, Hogeun,Lee, Bokkwon,Park, Misun,Lee, Hoanjong,Lee, Jina,Kim, Seonghan 대한감염학회 2009 감염과 화학요법 Vol.41 No.3
A multiplex PCR method has been developed to classify extended spectrum B-lactamase (ESBL) and Plasmid-mediated AmpC B-lactamase (PABL). This method consists of the use of two four-multiplex PCRs for the detection of TEM, OXA, SHV, CTX-M, CMY, and DHAtype B-lactamases. We have compared findings from the use of conventional detection methods with that of this newly developed typing method, In testing for 73 ESBL-producing and PABL-producing isolates, 100% of the isolates were correctly identified as previously characterized types and, 44 types of B-lactamases were additionally identified from 33 isolates. This assay not only reduces the time for classification but also increases the accuracy for detection.