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Han, Sei-Myoung,Han, Sang-Hun,Coh, Ye-Rin,Jang, Goo,Chan Ra, Jeong,Kang, Sung-Keun,Lee, Hee-Woo,Youn, Hwa-Young Nature Publishing Group 2014 Experimental and molecular medicine Vol.46 No.6
<P>Mesenchymal stem cells (MSCs) are attractive candidates for clinical repair or regeneration of damaged tissues. Oct4 and Sox2, which are essential transcription factors for pluripotency and self-renewal, are naturally expressed in MSCs at low levels in early passages, and their levels gradually decrease as the passage number increases. Therefore, to improve MSC proliferation and stemness, we introduced human Oct4 and Sox2 for conferring higher expansion and differentiation capabilities. The Oct4-IRES-Sox2 vector was transfected into human adipose tissue MSCs (ATMSCs) by liposomal transfection and used directly. Oct4 and Sox2 were successfully transfected into ATMSCs, and we confirmed maintenance of MSC surface markers without alterations in both red fluorescent protein (RFP) (control) and Oct4/Sox2-ATMSCs. Enhanced proliferative activity of Oct4/Sox2-ATMSCs was shown by WST-1 assay, and this result was further confirmed by cell counting using trypan blue exclusion for a long period. In addition, FACs cell cycle analysis showed that there was a reduction in the fraction of Oct4/Sox2-ATMSCs in G1 with a concomitant increase in the fraction of cells in S, compared with RFP-ATMSCs. Increased levels of cyclin D1 were also seen in Oct4/Sox2-ATMSCs, indicating acceleration in the transition of cells from G1 to S phase. Furthermore, Oct4/Sox2-overexpressing ATMSCs showed higher differentiation abilities for adipocytes or osteoblasts than controls. The markers of adipogenic or osteogenic differentiation were also upregulated by Oct4/Sox2 overexpression. The improvement in cell proliferation and differentiation using Oct4/Sox2 expression in ATMSCs may be a useful method for expanding the population and increasing the stemness of ATMSCs.</P>
말티즈에서 폭풍공포증 치료를 위한 클로미프라민, 알프라졸람의 투여가 행동 수정에 미치는 임상 1례
Sei-Myoung Han,Jae-Hyeon Cho,Chung-Hui Kim 한국동물위생학회 2023 韓國家畜衛生學會誌 Vol.46 No.2
An 8-year-old female neutered Maltese dog exhibited signs of serious thunderstorm phobia including panting, wandering, shivering, hiding, crying or whining, or trying to escape from the house. In this study, we administered clomipramine and alprazolam and observed behavioral improvement for 3 years. Clinical symptoms measured according to the storm phobia assessment (SPA) were significantly high with an initial total score of 19 points. However, it improved to a total score of 8 on day 176 after drugs administration and returned to the normal level with the total score of 5 points on the 983 days. When the symptoms for the first 4 treatments and the last 4 treatments were compared in 2 groups, the total score of all clinical symptoms decreased to −62.83% in the last 4 treatments. This study found that treatment with alprazolam and clomipramine was associated with a decrease in clinical signs of storm anxiety, suggesting that two drugs would be effective in reducing symptoms caused by anxiety, thunderstorm phobia, and noise phobia. In this follow up study, we hope that more studies would be conducted on behavior modification for treatment of storm phobia in veterinary medicine.
The wound healing effect of topical povidone ointment in dogs
Sei-Myoung Han,Hwan-Goo Kang 한국예방수의학회 2024 예방수의학회지 Vol.48 No.1
In this study, we evaluated wound healing following the use of 3% topical povidone ointment in dogs with minor wounds and evaluated it as a new wound treatment applicable to veterinary medicine. Twenty-six dogs that had suffered minor wounds such as abrasions, punctures, and incised wound within 8 hours were recruited, and all treatments were performed with the consent of their owners. Vitality, behavioral symptoms, and dermal symptoms due to wounds were evaluated between the povidone ointment group (n=18) and the control group (chlorhexidine group, n=8). As a result of evaluating changes in vitality and behavior, the povidone ointment group showed improvement to 1.94 (±2.53), 0.61 (±1.34), and 0.39 (±1.24) while the chlorhexidine group showed improvement to 2.00 (±1.6), 0.87 (±0.99), and 0.86 (±0.99) on days 0, 3, and 7 respectively, showing similar improvement in symptoms between the two groups (p>0.05). When observing wound symptoms, most of the swelling and inflammation improved within 3 days. The averages for the chlorhexidine group on days 0, 3, and 7 were 4.88 (±2.85), 2.88 (±2.64), and 2.38 (±2.39), and for the povidone ointment group, 5.55 (±3.22), 3.83 (±3.07), and 1.38 (±1.61), respectively. The wound healing effect of the povidone ointment group was similar to that of the chlorhexidine group (p>0.05). Through this study, we believe that topical povidone ointment can be applied to various wounds by replacing chlorhexidine.
한세명(Sei-Myoung Han),김향미(Hyang-Mee Kim) 한국동물보건학회 2022 한국동물보건학회지 Vol.1 No.1
Claw fracture disease can often be encountered in veterinary medicine, but the rate of visits related to claw diseases in South Korea has not been accurately investigated. This case is about systemic inflammation after a toenail fracture. A 2-year-old male, a Pomeranian dog visited the animal medical center with acute vomiting and anorexia after undergoing declawing for a fractured claw at another hospital. To evaluate the cause of vomiting, blood tests, X-rays, and abdominal ultrasonography were performed. In blood tests, C-reactive protein and D-dimer levels were 105 mg/L and 560.05 ng/ml, respectively, which were higher than normal, and the cPL kit test was negative. At the time of admission, the affected area was not properly protected and did not coalesce with a purulent exudate. It was judged that the cause of vomiting was an inflammatory reaction that progressed throughout the body. Therefore, an antibiotic injection and fluid treatment were performed during hospitalization, and the affected area was treated with sugar dressing 4 times a day. As the inflammation level gradually improved, the clinical symptoms also improved. Therefore, this case suggests the importance of correct postoperative management after initial claw damage and declawing from the animal health point of view.
반려동물 입양과정과 유기동물 발생에 대한 관련성 고찰 및 해결방안 모색
한세명(Sei-Myoung Han),김민철(Min-Chul Kim),김성호(Seong-Ho Kim),이수정(Soo-Jung Lee),오희경(Hee-Kyung Oh) 한국동물보건학회 2022 한국동물보건학회지 Vol.1 No.2
As the companion animal market has grown rapidly, social awareness of affection for animals and animal protection has also developed rapidly. However, as a result, various social problems (unethical puppy mills, conflicts between companions and non-companions, increase in abandoned animals, dog bite accidents, etc.) are occurring. In particular, social costs are increasing due to the increase in lost and abandoned animals, so it is necessary to reduce lost and abandoned animals and promote adoption. However, this problem is not simply a matter of the increase in companion animals. It is related to social problems such as the factory breeding business, the adoption of companion animals without sufficient understanding of companion animals, the perception that abandoned animals are not healthy, lack of guidance in the adoption process, and dissolution of adoption. Therefore, in this review, domestic and overseas cases were compared and necessary improvement measures were found in the process of adopting a companion animal. It is expected that the occurrence of abandoned animals will be reduced by making animal registration mandatory with a built-in wireless identification device. In addition, it is necessary to prevent the impulsive adoption of a companion animal by providing an education system for adopters so that they can adopt with sufficient education and consideration period. It is also important to eliminate prejudice against the adoption of abandoned animals by checking the health of animals under protection from animal shelters. Furthermore, it is necessary to make efforts to lead to the formation of a social community and the promotion of the adoption of abandoned animals by operating an education program that continuously spreads companion culture based on the local community.
Han, Sang-Hun,Jang, Goo,Bae, Bo-Kyoung,Han, Sei-Myoung,Koh, Ye-Rin,Ahn, Jin-Ok,Jung, Woo-Sung,Kang, Sung-Keun,Ra, Jeong-Chan,Lee, Hee-Woo,Youn, Hwa-Young Published for the International Federation for Cel 2014 Cell biology international Vol.38 No.10
<P>Enhancing the proliferative capacity of mesenchymal stem cells (MSCs) is critical for increasing their therapeutic potential in a variety of diseases. We hypothesized that lentivirus-mediated overexpression of canine octamer-binding transcription factor 4 (OCT4) might influence the proliferation of canine adipose tissue-derived MSCs (cATMSCs). cOCT4-cATMSCs were generated by transducing cATMSCs with a cOCT4-lentiviral vector. Increased expression of cOCT4 was confirmed using RT-PCR and immunoblotting. Immunophenotypic characterization using flow cytometry indicated that the CD29, CD44, CD73, CD90, and CD105 surface markers were highly expressed by both cOCT4- and mock-transduced cATMSCs (mock-cATMSCs), whereas the CD31 and CD45 markers were absent. We performed the osteogenic differentiation assay to evaluate the effects of cOCT4 overexpression on the osteogenic differentiation potential of cATMSCs. The results showed that cOCT4-cATMSCs had a much higher potential for osteogenic differentiation than mock-cATMSCs. Next, the proliferative capacities of cOCT4- and mock-cATMSCs were evaluated using a WST-1 cell proliferation assay and trypan blue exclusion. cOCT4-cATMSCs showed a higher proliferative capacity than mock-cATMSCs. Cell cycle analysis indicated that overexpression of cOCT4 in cATMSCs induced an increase in the proportion of cells in S and G2/M phases. Consistent with this, immunoblot analysis showed that cyclin D1 expression was increased in cOCT4-cATMSCs. In conclusion, our results indicate that lentivirus-mediated overexpression of cOCT4 increased the proliferative capacity of cATMSCs. OCT4-mediated enhancement of cell proliferation may be a useful method for expanding MSC population rapidly without loss of stemness.</P>