Expression of the Na⁺-HCO₃⁻cotransporter and its role in pH_iregulation in guinea pig salivary glands

Li, Jingchao,Koo, Na-Youn,Cho, Ik-Hyun,Kwon, Tae-Hwan,Choi, Se-Young,Lee, Sung J.,Oh, Seog B.,Kim, Joong-Soo,Park, Kyungpyo American Physiological Society 2006 American journal of physiology, Gastrointestinal a Vol.291 No.6

<P>Patterns of salivary HCO3<SUP>−</SUP>secretion vary and depend on species and gland types. However, the identities of the transporters involved in HCO3<SUP>−</SUP>transport and the underlying mechanism of intracellular pH (pHi) regulation in salivary glands still remain unclear. In this study, we examined the expression of the Na<SUP>+</SUP>-HCO3<SUP>−</SUP>cotransporter (NBC) and its role in pHiregulation in guinea pig salivary glands, which can serve as an experimental model to study HCO3<SUP>−</SUP>transport in human salivary glands. RT-PCR, immunohistochemistry, and pHimeasurements from BCECF-AM-loaded cells were performed. The amiloride-sensitive Na<SUP>+</SUP>/H<SUP>+</SUP>exchanger (NHE) played a putative role in pHiregulation in salivary acinar cells and also appeared to be involved in regulation in salivary ducts. In addition to NHE, NBC also played a role in pHiregulation in both acini and ducts. In the parotid gland, NBC1 was functionally expressed in the basolateral membrane (BLM) of acinar cells and the luminal membrane (LM) of ducts. In the submandibular gland, NBC1 was expressed only in the BLM of ducts. NBC1 expressed in these two types of salivary glands takes up HCO3<SUP>−</SUP>and is involved in pHiregulation. Although NBC3 immunoreactivity was also detected in submandibular gland acinar cells and in the ducts of both glands, it is unlikely that NBC3 plays any role in pHiregulation. We conclude that NBC1 is functionally expressed and plays a role in pHiregulation in guinea pig salivary glands but that its localization and role are different depending on the type of salivary glands.</P>

국산·수입산 동부를 이용한 모시잎송편의 영양성분비교

김영섭,김소민,오지연,황진봉,이기택,신정아,김상천,김세나,김행란,황영,최용민 忠北大學校 農業科學硏究所 2015 農業科學硏究 Vol.31 No.2

Production of Bacterial Cellulose by Acetobacter sp. A9 in Shaking Cultures

Son,Hong Joo,Jeong,Se Na,Kim,Keun Ki,Kim,Han Soo,Kim,Yong Gyun 한국생명과학회 2001 한국생명과학회 학술발표회 Vol.31 No.-

Bacterial cellulose (BC) is essentially a high-value speciality chemical with specific applications and usage. Because of its high tensile strength and water holding capacity, BC has been used as a raw material for producing high fidelity acoustic speaker, high quality paper and diet and dessert foods. In this study, the optimum fermentation condition for the production of cellulose by a newly isolated Acetobacter sp. A9 was determined by shaken cultures. The strain was able to produce cellulose at 25-30℃ with a maximum at 30℃. Cellulose production occurred at pH 4.5-7.5 with a maximum at pH 6.5. The improved medium composition was 4%(w/v) glucose, 0.1%(w/v) yeast extract, 0.7%(w/v) polypeptone and 0.8%(w/v) Na₂HPO₄·12H₂O. Under this culture condition, cellulose of 3.8 g/l was produced after 7 d of cultivation, although this strain produced only 2.2 g/l in the standard medium. The addition of ethanol to the improved medium was enhanced cellulose production. In an improved medium containing 1.4% (v/v) ethanol, cellulose production was 15.2 g/l, which was about 4 times higher than that without ethanol. Addition of ethanol was found to eliminate the spontaneous mutation of Acetobacter sp. A9.

국가직업능력표준 실용화를 위한 제도화 방안

나승일,김주섭,김주일,정연앙,구자길,김강호,문세연 한국농업교육학회 2007 농업교육과 인적자원개발 Vol.39 No.1

이 연구의 목적은 일-교육훈련-자격이 연계될 수 있도록 노동부의 국가직업능력표준을 국가차원에서 개발·운영·관리될 수 있는 제도화 방안을 마련하는데 있었다. 연구목적 달성을 위해 문헌고찰 및 내용분석, 포커스그룹인터뷰를 실시하였고, 연구결과의 타당성 확보를 위해 전문가협의회 및 공청회를 실시하였다. 국가직업능력표준 제도화는 표준의 실용화를 제고할 수 있도록 관련용어 정의, 총괄기구, 표준개발 및 승인, 표준 활용에 관한 인프라 구축에 필요한 사항이 규정되도록 관계 법령을 정비하는 것이다. 표준 제도화를 위해 다음과 같은 사항이 관계 법령에 명시되도록 해야 한다. ① 표준 제도화를 위해 직업, 직업능력, 직무, 표준 등을 관련법에 정의해야 한다. ② 국가직업능력표준 관련 부처 및 다양한 이해당사자의 참여를 기반으로 한 국가직업능력표준 사업 전담 조직을 설치하고, 표준사업에 대한 자문과 심의를 담당할 수 있는 위원회를 구축한다. ③ 다양한 산업분야 단체가 표준개발의 주체가 될 수 있도록 지원하고, 표준의 수준 설정을 통해 직종 내 수직적 경력개발을 촉진하며, 개발된 표준에 대한 지속적인 관리와 현장적합성을 승인하는 체제를 구축해야 한다. ④ 국가직업능력표준은 산업현장에서는 인력을 채용하고 관리하는 기준으로, 교육훈련에서는 교육과정을 개발하고 교과목 및 교재를 개발하며 현 교육과정을 평가하는 기준으로, 자격체계와는 표준의 자격화, 새로운 자격설계, 자격의 출제기준으로 활용될 수 있도록 지원해야 한다. 이를 위해 국가기술자격법, 근로자직업능력개발법, 산업인력공단법 등 관계 법령을 개정하거나 국가직업능력표준에 관한 특별법을 제정할 필요가 있다. 한편, 국가직업능력표준 제도화 단계에 따른 구체적인 추진과제와 이를 실현하기 위한 정책적 제언을 제시하였다. The purpose of this study was to develop the institutionalization that NOS(National Occupational Standard) was developed, operated and managed for linking with work, training and qualification. To achieve the purpose, literature reviews, contents analysis and focus group interview were conducted, and experts council and a public hearing were executed. The institutionalization is the legislation consolidation to regulate details related infra construction as follows for improving utilization of NOS. ① It should be defined occupation, occupational competency, job, standards and so forth on the related laws. ② A organization which take exclusive charge with and a council which consult and deliberate on NOS projects should be established through the cooperation between stakeholder and government organization. ③ It should be supported that various industrial parties including SHRDC are the main body for development of NOS, promoted vertical career development by occupation through establishing level of NOS, and established system that manage continually and approve practical application of NOS. ④ It should be supported that NOS is used as a criterion for recruiting and managing workforce, a criterion for developing and evaluating curriculum and educational materials in training, having qualification for itself, designing for new qualification, and a criterion for setting questions for a qualifying examination. To improve utilization of NOS, It need to be consolidated the related laws including National Technical Qualifications Act and Workers' Vocational Competency Development Act or to be enacted a special law on NOS. Moreover, this study suggested action plan according to the stages of institutionalization, and political proposal for effective actualization of action plan.

10MW 행원 풍력발전단지와 연계된 성산변전소의 모선전압 및 고조파 분석

김세호,나경윤 제주대학교 공과대학 첨단기술연구소 2004 尖端技術硏究所論文集 Vol.15 No.1

In recent years wind turbine technology has undergone the rapid development in response to the demands for increased use of renewable sources of energy. Using wind turbines for production of electrical energy requires reliable operation. The increased share of wind power in electrical system makes it necessary to have grid-friendly interfaces between the wind turbines and the grid in order to maintain power quality. Increasingly wind turbines are being connected into electricity distribution system. The grid-connected wind power stations have many impacts on power systems such as voltage variations, harmonics. The paper investigates the influences of grid-connected wind power generation system on substation bus voltage in Hang-won, Jeju.

행원풍력발전단지의 전압 및 역률분석

김세호,나경윤 제주대학교 공과대학 첨단기술연구소 2004 尖端技術硏究所論文集 Vol.15 No.2

In recent years wind turbine technology has undergone the rapid development in response to the demands for increased use of renewable sources of energy. Using wind turbines for production of electrical energy requires reliable operation. The increased share of wind power in electrical system makes it necessary to have grid-friendly interfaces between the wind turbines and the grid in order to maintain power quality. Increasingly wind turbines are being connected into electricity distribution system. The grid-connected wind power stations have many impacts on power systems such as voltage variations, harmonics. The paper investigates the influences of grid-connected wind power generation system on substation bus voltage and power factor

농업생명과학대학 교수가 활용하는 교수설계, 방법 및 전략

김기용,나승일,문세연 한국농업교육학회 2009 농업교육과 인적자원개발 Vol.41 No.1

이 연구의 목적은 농업생명과학대학 교수들이 과목 지도에서 활용하는 교수전략, 방법 및 전략을 확인하는데 있었다. 연구 목적을 달성하기 위해 총 46개 대학에 근무하는 1,471명의 교수 중 307명을 단순무선표집하여 설문조사를 실시하였다. 주요 연구 결과는 다음과 같다. 첫째, 농학계 대학 교수들은 교수능력을 개발하기 위한 기회가 부족한 편이며, 교육방법에 대한 학습경험이 적은 편이다. 둘째, 농학계 대학 교수들은 사전에 수업운영에 대한 구상은 하지만 실제적인 지도계획 수립이나 구체적인 자료준비는 부족하며, 수업을 위한 교안 작성자는 과반수에 지나지 않았다. 셋째, 농학계 대학 교수들은 강의법, 실험 및 실습 등의 한정된 수업방법을 주로 활용하나 최근에 강조되는 학습자 중심 교수방법들의 활용은 부족한 것으로 나타났고, 실제 수업 단계에서 특히 수업 도입이나 마무리 단계가 미흡한 것으로 나타났다. 넷째, 농학계 대학 교수들은 학생 강의평가 결과를 수업개선에 적극적으로 활용하지 않으며, 강의평가를 제외한 대학차원의 교수개선 지원이 부족하다. 이러한 결과에 기초하여 이 연구에서는 농업 및 농촌 현장과 연계된 교육, 교육목표에 적절한 교수설계, 학습자 중심의 교수방법 활용, 강의부담 경감, 교수 전문성 향상을 위한 기회 및 지원 제공 등의 개선방안을 제안하였다. The purpose of this study was to identify the instructional designs, teaching methods and strategies used by professors in the college of agriculture and life sciences in Korea. The population for this study was 1,471 professors of agriculture and life science colleges. 307 professors were randomly chosen from the population for the study. The survey was carried out by e-mail or in person. The major findings of this study were as follows: First, professors of colleges of agriculture and life sciences do not have many opportunities to improve their own teaching abilities. Also, they do not have many training experiences in taking pedagogy class, and attending seminars and workshops. Second, professors do not make an actual lesson plan or prepare enough learning aids materials on a class period base. Third, the professors rarely used the more learner-centered approaches. In addition, they usually emphasized on the "body stage" of each lesson which resulted in the unbalanced instruction in the "opening" and the "closing stages" of the lesson. Finally, professors execute the course evaluation, but the results of these evaluations are not being used for improvement of teaching and learning. Based on these results, some recommendations were made: the field-related education in agriculture, proper systematic design of the course teaching, use of more learner-centered teaching methods, building the constructive environment for the professors by reducing mandatory lectures and the balanced teaching from the opening to the closing in every instruction.

일본 전통문양의 특징에 관한 연구

김세나,오경화 한국의류산업학회 2003 한국의류산업학회지 Vol.5 No.5

The traditional culture has been developed for a long time with an unique property according to folk character, custom and environment within an area. Especially in 1906, when the westerners were interested in oriental cultures more and more, Paul Poiret presented Japanese traditional dress of napping sleeve coats and harem pants, henceforth japanese fashion theme began to rise. The Japanese style rose as an important fashion item of the modern fashion, with various fashion themes such as orientalism, ethnic, folklore, ecology, etc., needless to say of activities of Japanese designers. The revealed patterns on Japanese dress and it's ornament plays a role of expressive decoration as well as stand for the nation, the ideology of the age and aesthetic consciousness. For this reason, it is necessary to study Japanese traditional patterns expressed in modern fashion systematically. Therefore, in this research, the demonstrated Japanese traditional patterns on the modern fashion were divided into natural, artificial and complex design and were looked out the characteristic, furthermore it was studied in regard that Japanese patterns were how to apply in modern fashion. It was concluded that the characteristics of the Japanese traditional patterns presented in the modern fashion were as follows: first, creation of environmental friendly design using plenty of natural materials; second, expression of paint-like impression and beauty of margins by simplifying of patterns; third, expression of amusing design by using bright colors and print; finally, various patterns were spreaded out complicated on dress. The characteristics of Japanese traditional patterns in the above were expressed in the modern fashion.

소나무(Pinus densiflora) 목질을 이용한 수용액 중의 Cu(II)흡착

김하나,박세근,양경민,김영관 강원대학교 산업기술연구소 2007 産業技術硏究 Vol.27 No.A

Milled Korean pine (Pinus densiflora) wood was used to evaluate its adsorption capacity of Cu(Ⅱ) ions from aqueous solution by running a series of batch experiments. Prior to the tests, the milled woods were pretreated with 1N NaOH, 1N HNO_(3), and distilled water, respectively, to examine the effect of pretreatment. Within the tested pH range between 3 and 6, copper adsorption efficiency of NaOH-treated wood(96~99%) was superior to the HNO_(3)-treated wood(19~31%) and distilled water-treated wood(18~35%). The efficiency of copper removal by wood enhanced with increasing solution pH and reached a maximum copper ion uptake at pH 5~6. Adsorption behavior of copper onto both raw and HNO_(3)-treated woods was mainly attributed to interaction with carboxylic acid group. For NaOH-treated wood, carboxylate ion produced by hydrolysis or saponification was a major functional group responsible for Cu sorption. NaOH treatment of wood changed the ester and carboxylic acid groups into carboxylate group, whereas HNO_(3) treatment did not affect the production of functional groups which could bind copper. A pseudo second-order kinetic model fitted well for the sorption of copper ion onto NaOH-treated wood. A batch isotherm test using NaOH-treated wood showed that equilibrium sorption data were better represented by the Langmuir model than the Freundlich model.

도농간 교육격차 해소를 위한 농촌지역 유형별 교육혁신 추진과제 탐색

나승일,정철영,구자억,박행모,장호순,김진구,마상진,조단비,문세연 한국농업교육학회 2007 농업교육과 인적자원개발 Vol.39 No.1

이 연구는 교육혁신에 대한 이론적 고찰을 토대로 농촌지역 교육혁신 성공요소를 도출하고, 농촌의 지역특성을 고려하여 도농간 교육격차 해소를 위한 농촌지역 유형별 추진과제를 제시하는 데 목적이 있었다. 연구목적 달성을 위해 문헌고찰 및 사례연구를 실시하였고, 연구결과의 타당성 확보를 위해 전문가협의회를 실시하였다. 농촌지역 교육혁신 성공요소는 마을공부방 운영 등 26개가 최종적으로 도출되었다. 또한 농촌지역 교육혁신모형을 개발하여 목표와 전략을 수립하고, 이를 기초로 농촌지역 유형별 추진과제를 제시하였다. 농촌지역 교육혁신의 비전은 "도농간 교육격차 해소를 위한 교육혁신 실현으로 살고 싶은 농촌지역 구현" 으로 설정하고, 이를 달성하기 위한 목표는 도시수준의 교육기회 제공, 농촌형 수월성 교육 추구, 지역 인적자원개발로 설정하였다. 농촌지역 유형별 중점적으로 추진해야 할 과제는 발전 농촌지역은 마을 공부방 운영 및 학습도우미 지원, 중간수준 농촌지역은 교육환경 개선, 장학금 지원 및 다양한 교육기회 제공, 저개발 농촌지역은 기존 지원 유지, 적극적 학습 지원 및 교원의 근무여건 개선, 도농복합 농촌지역에서는 교통편의 제공, 학습기회 개선 및 학부모의 교육참여 유도 등이 선정되었다. 그리고 농촌지역 유형별 중점 추진과제에 대한 단계별 로드맵과 이를 적용하기 위한 정책적 제언을 제시하였다. The purpose of this research was to derive success factors and explore actions plan for the education innovation of rural areas for releasing education gaps between urban and rural areas. For the purpose, the researcher carried out literature reviews and case analysis were conducted, and experts council were executed. As a result, 26 success factors for the education innovation of rural area were derived. And, this study suggested the education innovation model of rural area including goals and strategies to identify action plan for the education innovation by types of rural areas. The vision of the education innovation model was making the place pleasant to live in by doing education innovation for releasing education gaps between urban and rural areas. The goals of the education innovation model were to provide urban-level educational opportunities, to pursue rural-customized education for excellency and to develope local human resources. This study identified several core tasks corresponding to each rural area: additional establishment and operation of village study-rooms and support for learning assistant system for highly-developed rural areas; improvement of educational condition and provision of scholarship and various educational opportunities for middle-developed rural areas; maintenance of existing support, active learning-support and improvement of teachers' working conditions for lowly-developed rural areas; provision of traffic convenience, improvement of learning opportunities and promotion of parents' educational participation for urban-rural complex areas. Moreover, this study suggested roadmap according to action plan divided by types of rural areas, and political proposal for effective actualization of that.