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Analysis of the Soluble Human Tooth Proteome and Its Ability to Induce Dentin/Tooth Regeneration
Chun, So Young,Lee, Hyo Jung,Choi, Young Ae,Kim, Kyung Min,Baek, Sang Heum,Park, Hyo Sang,Kim, Jae-Young,Ahn, Jung-Mo,Cho, Je-Yeol,Cho, Dong-Woo,Shin, Hong-In,Park, Eui Kyun Mary Ann Liebert 2011 Tissue engineering. Part A Vol.17 No.1
Chun, Jinyoung,Seo, Sang Woo,Jung, Gyoo Yeol,Lee, Jinwoo Royal Society of Chemistry 2011 Journal of materials chemistry Vol.21 No.18
<P>The simple and efficient separation of histidine-tagged (His-tagged) proteins using nickel ferrite (NiFe<SUB>2</SUB>O<SUB>4</SUB>) nanoparticle clusters (NPCs) is described in this article. While nanostructured materials containing Ni<SUP>2+</SUP> ions for efficient separation were generally synthesized <I>via</I> complex synthetic procedures, the NiFe<SUB>2</SUB>O<SUB>4</SUB> nanoparticle clusters used in this work were simply synthesized by a one-pot hydrothermal polyol process. Their large surface area (105.0 m<SUP>2</SUP> g<SUP>−1</SUP>) and pore volume (0.32 cm<SUP>3</SUP> g<SUP>−1</SUP>) provide sufficient sites for the separation of His-tagged proteins. The high magnetic saturation value (41.3 emu g<SUP>−1</SUP>) and superparamagnetic property of these nanoparticle clusters lead to a more efficient magnetically recyclable separation of His-tagged proteins. We confirmed that the binding capacity and selective separation ability seen in the first separation were strongly maintained for up to 7 cycles.</P> <P>Graphic Abstract</P><P>The simple and efficient purification of histidine-tagged (His-tagged) proteins using nickel ferrite (NiFe<SUB>2</SUB>O<SUB>4</SUB>) nanoparticle clusters is described. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c0jm04089d'> </P>
Persistence of Chinese Milk Vetch(Astragalus sinicus L.) Seed in Naturally Reseeded CMV-Rice Field
Sang-Yeol Kim,Sung-Hwan Oh,Jong-Hee Lee,Jun-Hyeon Cho,Kuk-Hyun Jeong,Ji-Yoon Lee,Sung-Tae Park,You-Chun Song,Un-Sang Yeo,Jeong-Il Kim,Hang-Won Kang 한국작물학회 2010 한국작물학회 학술발표대회 논문집 Vol.2010 No.04
Field experiments were conducted to investigate persistence of Chinese milk vetch(CMV) seed under naturally reseeded CMV-rice cropping systems during the period of 2007~2009. Persistence of the CMV seeds were evaluated in the naturally reseeded CMV-rice field on different tillage methods and soil depths based on the seed number recovered and the seed longevity in soil. Field observation in the naturally reseeded CMV rice field showed that as many as 917~2,185 CMV seeds m-2 were found from 0~15cm soil depth in the rotary tillage and 250~10,105 CMV seeds in minimum tillage rice cultivation in autumn. The recovered seed germinated 25~33%, 23~43% but still had high percentage of hard seed having 64~72% and 51~77%. Field experiment showed that the CMV seedling still emerged even after 2 years of continuous destructive killing of emerged CMV plant by rotary tillage in naturally reseeded CMV-rice field, indicating that CMV seeds persistent at least two years in rice field.
( Sung Woo Chun ),( Jae Yeol Jang ),( Dae Won Park ),( Hee Chul Woo ),( Jong Shik Chung ) 한국화학공학회 1997 Korean Journal of Chemical Engineering Vol.14 No.3
The catalytic performance of some metal oxides in the selective oxidation of H₂S in the stream containing water vapor and ammonia was investigated in this study. Among the catalysts tested, Co₃O₄/SiO₂was the most promising catalyst for practical application. It showed very small amount of SO₂ emission even in the presence of excess water and ammonia. The solid product formed in the reaction was a mixture of elemental sulfur, ammonium thiosulfate and ammonium sulfate.
Sung Min Nam,Jong Hee Choi,Sun-Hye Choi,Hee-Jung Cho,Yeon-Jin Cho,Hyewhon Rhim,Hyoung-Chun Kim,Ik-Hyun Cho,Do-Geun Kim,Seung-Yeol Nah 고려인삼학회 2021 Journal of Ginseng Research Vol.45 No.3
Background: We recently showed that gintonin, an active ginseng ingredient, exhibits antibrain neurodegenerative disease effects including multiple target mechanisms such as antioxidative stress and antiinflammation via the lysophosphatidic acid (LPA) receptors. Amyotrophic lateral sclerosis (ALS) is a spinal disease characterized by neurodegenerative changes in motor neurons with subsequent skeletal muscle paralysis and death. However, pathophysiological mechanisms of ALS are still elusive, and therapeutic drugs have not yet been developed. We investigate the putative alleviating effects of gintonin in ALS. Methods: The G93A-SOD1 transgenic mouse ALS model was used. Gintonin (50 or 100 ㎎/㎏/day, p.o.) administration started from week seven. We performed histological analyses, immunoblot assays, and behavioral tests. Results: Gintonin extended mouse survival and relieved motor dysfunctions. Histological analyses of spinal cords revealed that gintonin increased the survival of motor neurons, expression of brain-derived neurotrophic factors, choline acetyltransferase, NeuN, and Nissl bodies compared with the vehicle control. Gintonin attenuated elevated spinal NAD(P) quinone oxidoreductase 1 expression and decreased oxidative stress-related ferritin, ionized calcium-binding adapter molecule 1-immunoreactive microglia, S100b-immunoreactive astrocyte, and Olig2-immunoreactive oligodendrocytes compared with the control vehicle. Interestingly, we found that the spinal LPA1 receptor level was decreased, whereas gintonin treatment restored decreased LPA1 receptor expression levels in the G93A-SOD1 transgenic mouse, thereby attenuating neurological symptoms and histological deficits. Conclusion: Gintonin-mediated symptomatic improvements of ALS might be associated with the attenuations of neuronal loss and oxidative stress via the spinal LPA1 receptor regulations. The present results suggest that the spinal LPA1 receptor is engaged in ALS, and gintonin may be useful for relieving ALS symptoms.
Zoom-in X-ray Micro Tomography System
Chun, In-Kon,Lee, Sang-Chul,Park, Jeong-Jin,Cho, Min-Hyoung,Lee, Soo-Yeol The Korean Society of Medical and Biological Engin 2005 의공학회지 Vol.26 No.5
We introduce an x-ray micro tomography system capable of high resolution imaging of a local region inside a small animal. By combining two kinds of projection data, one from a full field-of-view (FOV) scan of the whole body and the other from a limited FOV scan of the region of interest, we have obtained zoomed-in images of the region of interest without any contrast a nomalies. We have integrated a micro tomography system using a micro-focus x-ray source, a $1248\times1248$ flat-panel x-ray detector, and a precision scan mechanism. Using the cross-sectional images taken with the zoom-in micro tomography system, we measured trabecular thicknesses of femur bones in postmortem rats. To compensate the limited spatial resolution in the zoom-in micro tomography images, we used the fuzzy distance transform for the calculation of the trabecular thickness. To validate the trabecular thickness measurement with the zoom-in micro tomography images, we compared the measurement results with the ones obtained from the conventional micro tomography images of the extracted bone samples.
Chun, Hae-Jin,Lee, Choong-Yeol,Lee, Jin-Woo,Sung, Yun-Hee,Kim, Sung-Eun,Kim, Young-Sick,Shin, Mal-Soon,Kim, Chang-Ju,Lee, Hye-Jung,Kim, Dong-Hee Kyung Hee Oriental Medicine Research Center 2010 Oriental pharmacy and experimental medicine Vol.10 No.3
Clematis chinensis is the root of Clematis chinensis OSBECK and is classified in Ranunculaceae. Clematis chinensis is a traditional medicinal herb possesses analgesic, diuretic, anti-tumorigenic, and anti-inflammatory effects. In this study, the effect of aqueous extract of Clematis chinensis against lipopolysaccharide-induced inflammation was investigated in mouse BV2 microglial cells. The aqueous extract of Clematis chinensis at the respective concentration was treated one hour before the lipopolysaccharide treatment in mouse BV2 microglial cells. From the present results, pre-treatment with the aqueous extract of Clematis chinensis suppressed prostaglandin E2 synthesis and nitric oxide production by inhibiting on the lipopolysaccharide-stimulated cyclooxygenase-2 and inducible nitric oxide synthase expressions in mouse BV2 microglial cells. The present study suggests that Clematis chinensis may offer a valuable mean of therapy for brain inflammatory diseases.
Sang-Yeol Kim,Woon-Ha Hwang,Sung-Hwan Oh,Jong-Hee Lee,Jun-Hyeon Cho,Kuk-Hyun Jeong,Ji-Yoon Lee,Sung-Tae Park,You-Chun Song,Un-Sang Yeo,Jeong-Il Kim,Hang-Won Kang 한국작물학회 2010 한국작물학회 학술발표대회 논문집 Vol.2010 No.04
Experiments were conducted to investigated the change of primary dormancy and viability of Chinese milk vetch(CMV) seed buried in soil both as seed and pod with seeds under CMV-rice cropping system during the period of 2007~2009. The freshly harvested CMV seed alone and pods with seeds were buried in rice field at 0, 5 and 10cm depths and determined change of seed dormancy and viability at one to three month intervals for 10 months. The CMV seed had high dormancy of 95%, showing only 4~5% germination at the beginning in June but the seed dormancy was gradually broken at rice harvest time in autumn, showing 25 to 35% for seed and 55 to 61% for pod with seeds. The viability loss was faster in the seed than in the pod with seeds regardless of depths of placement in the soil base on decayed seeds. Also the seed placed on the soil surface lost viability faster than the 5~10 burial depths. However, appreciable number of CMV seeds still remained at even 4 months after burial in soil. These results indicate that seed dormancy was enable CMV plant to regenerate naturally from the remained soil seed bank at rice harvest time in autumn.