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Sang Yun Shin,Jin Seok Kim,Jong Keun Lim,Jee Sook Hahn,Woo Ick Yang,Chang Ok Suh 대한내과학회 2006 The Korean Journal of Internal Medicine Vol.21 No.2
Primary hepatic lymphoma is a rare disorder representing less than 1% of all extranodal lymphomas. Histological examination of a primary hepatic lymphoma usually reveals a diffuse large B-cell lymphoma; there have been few reports of primary hepatic mucosa
Gene editing platform in Cattle
Sang-Eun Hahn,Soo-Young Yum,Song-Jeon Lee,Choong-Il Lee,Hee-Soo Kim,Hyeong-Jong Kim,Woo-Jae Choi,Ji-Hyun Lee,Woo-Sung Lee,Goo Jang 한국수정란이식학회 2016 한국수정란이식학회 학술대회 Vol.2016 No.10
The CRISPR/Cas9 system is proved to be a powerful tool for knock-out and knock-in in various species. By introducing genetic materials of two components (Cas9 and small guide (sg) RNA) into cells or pronuclear of the fertilized embryo, gene editing occurs. Some studies reported that efficiency of gene editing would be increased as Cas9 was integrated into cells or animals since Cas9 is indispensable in the CRISPR/Cas9 system. Accordingly, the production of Cas9 expressing cattle may provide the broadly used gene editing platform in cattle. For this study, Cas9 and RFP genes were cloned into PiggyBac (PB) transposon system. PB-Cas9-RFP and transposase were microinjected into 1436 in vitro fertilized embryos and 241 blastocysts were formed. Blastocysts with RFP expression accounting for 14.1% of total formed blastocysts were selected and transferred into 5 recipient cow. After gestation periods, four transgenic cattle were delivered without any veterinary assistance. From a transgenic cattle, ear skin tissue was collected for primary culture. On those primary cells, sgRNAs in DNA form for various genes such as PRNP, RB1 and BLG were transfected as 2ug of sgRNA per 5x105 cells using Nucleo factor system (Neon®, invitrogen, program#16). As expected, every group of each sgRNA delivered was confirmed to be mutated by T7E1assay. Those data demonstrated that for the first time, transgenic cattle with Cas9 expression were born, grown up to date and will be avaluable resource for genome-editing in cattle. This work was supported by BK21PLUS Program for Creative Veterinary Science and Seoul Milk Coop (SNU550-20160004).
Achievements and lessons learned from the operation of KSTAR plasma control system upgrade
Hahn, Sang-hee,Penaflor, B.G.,Milne, P.G.,Bak, J.G.,Eidietis, N.W.,Han, H.,Hong, J.S.,Jeon, Y.M.,Johnson, R.D.,Kim, H.-S.,Kim, HeungSu,Kim, Y.J.,Kwon, G.I.,Lee, W.R.,Woo, M.H.,Sammuli, B.S.,Walker, M. Elsevier 2018 Fusion engineering and design Vol.130 No.-
<P><B>Abstract</B></P> <P>Results on the integration and the operation of the KSTAR plasma control system (PCS) upgrade are given. Real-time hardware, new realtime-capable operating system, and a brand-new data acquisition are assembled in order to extend the performance and compatibility with modern computer systems. The first full commissioning and eventual routine use performed in 2016 so that the system can now acquire more than 400 channels for more than 100 seconds of data with 5 kHz sampling. The performance test results are summarized, featuring In/Out streaming echo tests and the synchronization verifications. Examples of general performance improvements are demonstrated, and additional features added to the software are also described.</P>