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( Sung Hyun Ahn ),( Eun Sook Park ),( Yong Kwang Park ),( Jeong Han Kim ),( Doo Hyun Kim ),( Keo Heun Lim ),( Won Hyeok Choe ),( Soon Young Ko ),( So Young Kwon ),( Kyun Hwan Kim ) 대한간학회 2013 춘·추계 학술대회 (KASL) Vol.2013 No.1
Background: Emergence of drug-resistant hepatitis B virus (HBV) against the nucleos(t)ide analogues (NA) is a major problem for antiviral treatment in chronic hepatitis B patients. In this study, we analyzed the evolution of drug-resistant mutations and characterized the effects of rtA181T and rtI233V mutations on viral replication and drug-resistance. Methods: We performed a clonal analysis of the HBV polymerase gene from serum samples during viral breakthrough treated with lamivudine (LMV) and adefovir (ADV). Representative mutants were analyzed for in vitro drug susceptibility by southern blot. We constructed a series of mutant clones and determined the ability of replication and drug resistance. Results: Conserved mutations in rt204, rt181, rt236, and rt233 were identified during the viral breakthrough. In vitro study revealed that the effect of rtA181T mutation on viral replication and drug resistance is dependent on the mutations in overlapping surface gene. The rtA181T mutant harboring surface stop (rtA181T/sW172*) showed a decrease in viral replication and increase in drug resistance compared to the rtA181T mutant harboring surface mutation (rtA181T/sW172S). Moreover, the rtA181T/sW172* mutant exhibited a secretion defect of viral particles. The rtI233V mutation which emerged during ADV therapy reduced the viral replication and conferred resistance to ADV. However, the rtI233V mutation did not affect the viral replication and drug resistance of rtA181T/sW172* mutant. Conclusions: Our data suggest that the impact of rtA181T mutation on drug resistance is different according to the mutation status in corresponding surface gene. The rtI233V mutation affects the replication ability and drug resistance. Our observation suggests the need of genotypic analysis of overlapping surface gene to manage the antiviral drug resistance if clinical isolates harbor rtA181T mutation.
Wafer-scale arrays of epitaxial ferroelectric nanodiscs and nanorings
Han, Hee,Ji, Ran,Park, Yong Jun,Lee, Sung Kyun,Rhun, Gwenael Le,Alexe, Marin,Nielsch, Kornelius,Hesse, Dietrich,Gö,sele, Ulrich,Baik, Sunggi IOP Pub 2009 Nanotechnology Vol.20 No.1
<P>Wafer-scale arrays of well-ordered Pb(Zr<SUB>0.2</SUB>Ti<SUB>0.8</SUB>)O<SUB>3</SUB> nanodiscs and nanorings were fabricated on the entire area (10 mm × 10 mm) of the SrRuO<SUB>3</SUB> bottom electrode on an SrTiO<SUB>3</SUB> single-crystal substrate using the laser interference lithography (LIL) process combined with pulsed laser deposition. The shape and size of the nanostructures were controlled by the amount of PZT deposited through the patterned holes and the temperature of the post-crystallization steps. X-ray diffraction and transmission electron microscopy confirmed that (001)-oriented PZT nanostructures were grown epitaxially on the SrRuO<SUB>3</SUB>(001) bottom electrode layer covering the (001)-oriented single-crystal substrate. The domain structures of PZT nano-islands were characterized by reciprocal space mapping using synchrotron x-ray radiation. Ferroelectric properties of each PZT nanostructure were characterized by scanning force microscopy in the piezoresponse mode.</P>
Highly entangled hollow TiO<sub>2</sub> nanoribbons templating diphenylalanine assembly
Han, Tae Hee,Oh, Jun Kyun,Park, Ji Sun,Kwon, Se-Hun,Kim, Sung-Wook,Kim, Sang Ouk Royal Society of Chemistry 2009 Journal of materials chemistry Vol.19 No.21
<P>We introduce a biotemplating approach for creating highly entangled hollow TiO<SUB>2</SUB> nanoribbons by combining peptide assembly with an atomic layer deposition process. An aromatic peptide of diphenylalanine was readily assembled into a hierarchical organogel consisting of highly entangled nanoribbons. Unlike ordinary biomaterials, the peptide nanoribbon framework exhibited a high level of thermal stability, such that it may undergo the further functionalization process of vacuum deposition without significant damage to its nanoscale structure. A nanoscale layer of anatase TiO<SUB>2</SUB> was deposited on the nanoribbon framework by means of atomic layer deposition. After pyrolysis, a highly entangled nanotubular TiO<SUB>2</SUB> framework was created successfully. The highly entangled TiO<SUB>2</SUB> architecture exhibited UV-switchable wetting properties.</P> <P>Graphic Abstract</P><P>Owing to high thermal stability of diphenylalanine assembly, a nanoscale layer of TiO<SUB>2</SUB> could be deposited on the surface of the xerogel framework assembled from diphenylalanine <I>via</I> an atomic layer deposition process. After the calcination of the peptide framework, highly entangled hollow TiO<SUB>2</SUB> nanoribbons were successfully created. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=b819254e'> </P>
Reduced Graphene Oxide/Mesoporous TiO<sub>2</sub> Nanocomposite Based Perovskite Solar Cells
Han, Gill Sang,Song, Young Hyun,Jin, Young Un,Lee, Jin-Wook,Park, Nam-Gyu,Kang, Bong Kyun,Lee, Jung-Kun,Cho, In Sun,Yoon, Dae Ho,Jung, Hyun Suk American Chemical Society 2015 ACS APPLIED MATERIALS & INTERFACES Vol.7 No.42
<P>We report on reduced graphene oxide (rGO)/mesoporous (mp)-TiO<SUB>2</SUB> nanocomposite based mesostructured perovskite solar cells that show an improved electron transport property owing to the reduced interfacial resistance. The amount of rGO added to the TiO<SUB>2</SUB> nanoparticles electron transport layer was optimized, and their impacts on film resistivity, electron diffusion, recombination time, and photovoltaic performance were investigated. The rGO/mp-TiO<SUB>2</SUB> nanocomposite film reduces interfacial resistance when compared to the mp-TiO<SUB>2</SUB> film, and hence, it improves charge collection efficiency. This effect significantly increases the short circuit current density and open circuit voltage. The rGO/mp-TiO<SUB>2</SUB> nanocomposite film with an optimal rGO content of 0.4 vol % shows 18% higher photon conversion efficiency compared with the TiO<SUB>2</SUB> nanoparticles based perovskite solar cells.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/aamick/2015/aamick.2015.7.issue-42/acsami.5b06171/production/images/medium/am-2015-06171k_0007.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/am5b06171'>ACS Electronic Supporting Info</A></P>
Han, Jee Eun,Lee, Seungki,Jeong, Dae Gwin,Yoon, Sun-Woo,Kim, Doo-Jin,Lee, Moo-Seung,Kim, Hye Kwon,Park, Sung-Kyun,Kim, Ji Hyung,Park, Se Chang Elsevier 2017 Journal of global antimicrobial resistance Vol.11 No.-
<P><B>Abstract</B></P> <P><B>Objectives</B></P> <P>This study aimed to determine the complete genome sequence of multidrug-resistant <I>Staphylococcus sciuri</I> strain SNUDS-18 isolated from a farmed duck in South Korea.</P> <P><B>Methods</B></P> <P>Genomic DNA was sequenced using a PacBio RS II system. The obtained genome was annotated and antimicrobial resistance and virulence genes were identified.</P> <P><B>Results</B></P> <P>The sequenced genome possessed a <I>mecA</I> homologue (<I>mecA1</I>) that was almost identical to that of other oxacillin-susceptible <I>S. sciuri</I> strains, whereas the staphylococcal cassette chromosome <I>mec</I> (SCC<I>mec</I>) was not detected. Moreover, various antimicrobial resistance genes conferring resistance to β-lactams, aminoglycosides, phenicols, tetracycline and macrolide–lincosamide–streptogramin B (MLS<SUB>B</SUB>) antimicrobials were identified.</P> <P><B>Conclusions</B></P> <P>The SNUDS-18 genome and its associated genomic data will provide important insights into the biodiversity of the <I>S. sciuri</I> group as well as valuable information for the control of this potential pathogen.</P>
Sung-Ryoul Kim,Jae-Woo Kwak,Sung-Ka Lee,Seung-Gon Jung,Man-Seung Han,Bang-Sin Kim,Min-Suk Kook,Hee-Kyun Oh,Hong-Ju Park 대한구강악안면외과학회 2012 대한구강악안면외과학회지 Vol.38 No.1
Introduction: This study was conducted to evaluate ssrA expression resulting from adaptation of Escherichia coli (E. coli) to oral pathogens through signal exchange. Materials and Methods: Human cell lines Hep2 and HT29, wild-type E. coli (WT K-12), ssrA knock-out E. coli (Δ K-12), and Scleropages aureus (S. aureus) were used. A single culture consisting of Hep2, HT29, WT K-12, and Δ K-12, and mixed cultures consisting of Hep2 and WT K-12, Hep2 and Δ K-12, WT K-12 and S. aureus, Δ K-12 and S. aureus, and Hep2, WT K-12, and S. aureus were prepared. For HT29, a mixed culture was prepared with WT K-12 and with WT K-12 and S. aureus. Total RNA was extracted from each culture with the resulting expression of ssrA, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), and p53 was evaluated by Reverse transcription polymerase chain reaction (RT-PCR). Results: The expression of ssrA in a single culture of WT K-12 was lower than that observed in the mixed culture of WT K-12 with S. aureus. Greater ssrA expression was observed in the mixed culture of WT K-12 with Hep2 than in the single culture of WT K-12. The expression of NF-κB was higher in the mixed culture of Hep2 with Δ K-12 than that in the mixed culture of Hep2 with WT K-12, and was lowest in the single culture of Hep2. The expression of ssrA was higher in the mixed culture of WT K-12 with Hep2 and S. aureus than in the mixed culture of WT K-12 with Hep2. Conclusion: These results suggest that ssrA plays an important role in the mechanism of E. coli adaptation to a new environment.