The Effects of Myrtle (Myrtus communis) and Clindamycin Topical Solution in the Treatment of Mild to Moderate Acne Vulgaris: A Comparative Split-Face Study

Salmanian, Mahboobeh,Shirbeigi, Laila,Hashem-Dabaghian, Fataneh,Mansouri, Parvin,Azizkhani, Mohammad,Alavi, Shiva,Ghobadi, Ali KOREAN PHARMACOPUNCTURE INSTITUTE 2020 Journal of pharmacopuncture Vol.23 No.4

Objectives: Although Acne vulgaris is a chronic skin disease, which its standard treatment causes therapeutic limitations and some common adverse effects, medicinal plants can be effective in treatment with low adverse effects as combination therapy. Myrtle (Myrtus Communis) has some beneficial properties, which has been administered topically and orally for some skin diseases in Persian medicine. This study aimed to compare the efficacy and safety of Myrtle formula and 1% clindamycin topical solution. Methods: This was a split-face clinical trial that was done on 55 patients with mild to moderate acne vulgaris for 16 weeks. The patients received topical Myrtle solution to the right side of the face (group 1) and clindamycin solution to the left side (group 2) twice daily for 12 weeks. All participants were examined for the acne severity index (ASI) and total acne lesions counting (TLC) at certain times during the study. Then, they stopped using them for four weeks. They also did not take the drug in the final four weeks of the study. Results: Forty-eight patients completed the study for 16 weeks; 40 (83.2%) patients were female and the rest of them were male. The mean age and standard deviation were 25.62 ± 7.62 years. After 12 weeks, the percentage changes of comedones, inflammatory lesions, ASI and TLC were significantly reduced in both groups (p < 0.001). The percentage change of inflammatory lesions and ASI decrease was significantly higher in the group 1 (p = 0.03). There was no significant difference in the incidence of side effects between the two groups. There was a more significant decrease in sebum percentage change in the group 1 (p = 0.003). Conclusion: Myrtle lotion was effective and safe for the treatment of mild to moderate acne vulgaris.

Tourism Planning

Maryam. Salmanian,Arezoo. Golkar,Mozafar Mohammadkhani,jafar.. Safakhah 세계문화관광학회 2010 Conference Proceedings Vol.11 No.0

According to tourism international organization report, countries income made by tourism has been increasingly developed in recent years. Nowadays tourism is the main power of economic development and growth in many countries and it diversifies the economy by providing opportunities and brings employment and income. According to statistics reported by this organization, tourism is of the main world industries next to oil and machinery industries and has had great role in solving economic problems by for example providing employment and income. This industry has been on main interest of governors in terms of macro investment due to its low cost and it is attempted to identify it. Our country in spite of having high capacities, has failed to keep pace with this growth and develop its internal and external tourism industry. In this study it is attempts to examine Iran‘s status with regard the fact that it has rich valuable natural and cultural treasuries by analyzing tourism importance in different countries. In addition, some recommendations are provided in order to improve some kinds of tourism which are more suitable for Iran with regard to its cultural and religious situation.

Tourism

Maryam,Salmanian,Arezoo,Golkar,Mozafar Mohammadkhani,jafar,Safakhah 세계문화관광학회 2010 Conference Proceedings Vol.11 No.0

According to tourism international organization report, countries income made by tourism has been increasingly developed in recent years. Nowadays tourism is the main power of economic development and growth in many countries and it diversifies the economy by providing opportunities and brings employment and income. According to statistics reported by this organization, tourism is of the main world industries next to oil and machinery industries and has had great role in solving economic problems by for example providing employment and income. This industry has been on main interest of governors in terms of macro investment due to its low cost and it is attempted to identify it. Our country in spite of having high capacities, has failed to keep pace with this growth and develop its internal and external tourism industry. In this study it is attempts to examine Iran‘s status with regard the fact that it has rich valuable natural and cultural treasuries by analyzing tourism importance in different countries. In addition, some recommendations are provided in order to improve some kinds of tourism which are more suitable for Iran with regard to its cultural and religious situation.

Immunogenicity of EIT chimeric protein expressed in transplastomic tobacco plants towards development of an oral vaccine against Escherichia coli O157:H7

Karimi, Farrokh,Mousavi, Amir,Salmanian, Ali Hatef,Alizadeh, Houshang,Rafati, Sima 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.4

Chloroplast genetic engineering offers an opportunity for high level expression and cost-effective recombinant protein production. Escherichia coli O157:H7 is one of the most important zoonotic pathogens causing hemorrhagic colitis (HC) and the life-threatening hemolytic-uremic syndrome in humans worldwide. The occurrence of zoonotic E. coli O157:H7 outbreaks in recent years has led to increased efforts in the development of safe and cost-effective immunogenic antigens against E. coli O157:H7. EspA and Tir/Intimin proteins are the important virulence factors which are encoded by the LEE locus of enterohemorrhagic E. coli. In this study, we hypothesized that the high level expression of the chimeric form of these effectors in chloroplasts and using tobacco transplastomic plants as an oral delivery system for the development of an edible-base vaccine would induce an immune response for the prevention of E. coli 0157:H7 attachment and colonization in animal model mice. The prokaryotic codonoptimized EIT protein was expressed in plastid genome via chloroplast transformation. Putative transplastomic plants were analyzed by PCR, and Southern blot analysis confirming chloroplast integration and homoplasmy in the T1 progeny. Immunoblotting and ELISA assays demonstrated that the EIT protein was expressed in chloroplasts and accumulated up to 1.4 % of total soluble protein in leaf tissue. In mice orally immunized with transplastomic tobacco plant leaves, high immunological responses (IgG and IgA specific antibodies) were detected in serum and feces. Finally, the challenging assay with E. coli O157:H7 in immunized mice showed reduced bacterial shedding.

Companies Life Cycle Stages and Capital Structure in Emerging Markets: Evidence from Iran

Mahdi Salehi,Vahab Rostami,Lida Salmanian 한국유통과학회 2013 유통과학연구 Vol.11 No.2

Purpose – The current research examines the effect of life cycle stages on capital structure of listed companies in Tehran Stock Exchange. Research design, data, methodology – By aid of 685 year‐company data, which collected from financial statements of companies during 2006‐2012, first, the companies, are classified into three groups including companies in growth, maturity and decline stages. After removing the companies, which were not in accordance with life cycle model, 86 companies were selected to test two main hypotheses of the research. Results – The results show that the capital structure of the sample companies is different in various life cycle stages. More investigation by LSD test also revealed that the total debt to total assets ratio means of the companies in growth stages were significantly different from those companies in maturity stages and those in growth stages had high level of debt to assets ratio. Conclusions – The result showed the average amount of the working capital for companies in three stages are significantly different and due to high level of operation of the companies in maturity and decline stages, these companies held high amount of working capital than those in the growth stages.

Expression of Escherichia coli Heat-labile Enterotoxin B Subunit (LTB) in Saccharomyces cerevisiae

Mohammad Ahangarzadeh Rezaee,Abbas Rezaee,Seyed Mohammad Moazzeni,Ali Hatef Salmanian,Yoko Yasuda,Kunio Tochikubo,Shahin Najar Pirayeh,Mohsen Arzanlou 한국미생물학회 2005 The journal of microbiology Vol.43 No.4

Heat-labile enterotoxin B subunit (LTB) of enterotoxigenic Escherichia coli (ETEC) is both a strong mucosal adjuvant and immunogen. It is a subunit vaccine candidate to be used against ETEC-induced diarrhea. It has already been expressed in several bacterial and plant systems. In order to construct yeast expressing vector for the LTB protein, the eltB gene encoding LTB was amplified from a human origin enterotoxigenic E. coli DNA by PCR. The expression plasmid pLTB83 was constructed by inserting the eltB gene into the pYES2 shuttle vector immediately downstream of the GAL1 promoter. The recombinant vector was transformed into S. cerevisiae and was then induced by galactose. The LTB protein was detected in the total soluble protein of the yeast by SDS-PAGE analysis. Quantitative ELISA showed that the maximum amount of LTB protein expressed in the yeast was approximately 1.9% of the total soluble protein. Immunoblotting analysis showed the yeast-derived LTB protein was antigenically indistinguishable from bacterial LTB protein. Since the whole-recombinant yeast has been introduced as a new vaccine formulation the expression of LTB in S. cerevisiae can offer an inexpensive yet effective strategy to protect against ETEC, especially in developing countries where it is needed most.

Immunogenicity of EIT chimeric protein expressed in transplastomic tobacco plants towards development of an oral vaccine against Escherichia coli O157:H7

Farrokh Karimi,Amir Mousavi,Houshang Alizadeh,Ali Hatef Salmanian,Sima Rafati 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.4

Chloroplast genetic engineering offers an opportunity for high level expression and cost-effective recombinant protein production. Escherichia coli O157:H7 is one of the most important zoonotic pathogens causing hemorrhagic colitis (HC) and the life-threatening hemolytic- uremic syndrome in humans worldwide. The occurrence of zoonotic E. coli O157:H7 outbreaks in recent years has led to increased efforts in the development of safe and cost-effective immunogenic antigens against E. coli O157:H7. EspA and Tir/Intimin proteins are the important virulence factors which are encoded by the LEE locus of enterohemorrhagic E. coli. In this study, we hypothesized that the high level expression of the chimeric form of these effectors in chloroplasts and using tobacco transplastomic plants as an oral delivery system for the development of an edible-base vaccine would induce an immune response for the prevention of E. coli 0157:H7 attachment and colonization in animal model mice. The prokaryotic codonoptimizedEIT protein was expressed in plastid genome via chloroplast transformation. Putative transplastomic plants were analyzed by PCR, and Southern blot analysis confirmingchloroplast integration and homoplasmy in the T1 progeny. Immunoblotting and ELISA assays demonstrated that the EIT protein was expressed in chloroplasts and accumulated up to 1.4 % of total soluble protein in leaf tissue. In mice orally immunized with transplastomic tobacco plant leaves, high immunological responses (IgG and IgA specific antibodies) were detected in serum and feces. Finally, the challenging assay with E. coli O157:H7 in immunized mice showed reduced bacterial shedding.

Poster Session : Innate Immunity ; Nasal immunization of mice with rotavirus VP2 DNA vaccine induces cellular and humoral immunity

( Shahin Ahmadian ),( Farzaneh Pourasgary ),( Zuhair Mohammad Hassan ),( Ali Hatef Salmanian ) 한국생화학분자생물학회 (구 한국생화학회) 2007 생화학분자생물학회 춘계학술발표논문집 Vol.2007 No.-

Expression of Escherichia coli Heat-labile Enterotoxin B Subunit (LTB) in Saccharomyces cerevisiae

Rezaee Mohammad Ahangarzadeh,Rezaee Abbas,Moazzeni Seyed Mohammad,Salmanian Ali Hatef,Yasuda Yoko,Tochikubo Kunio,Pirayeh Shahin Najar,Arzanlou Mohsen The Microbiological Society of Korea 2005 The journal of microbiology Vol.43 No.4

Heat-labile enterotoxin B subunit (LTB) of enterotoxigenic Escherichia coli (ETEC) is both a strong mucosal adjuvant and immunogen. It is a subunit vaccine candidate to be used against ETEC-induced diarrhea. It has already been expressed in several bacterial and plant systems. In order to construct yeast expressing vector for the LTB protein, the eltB gene encoding LTB was amplified from a human origin enterotoxigenic E. coli DNA by PCR. The expression plasmid pLTB83 was constructed by inserting the eltB gene into the pYES2 shuttle vector immediately downstream of the GAL1 promoter. The recombinant vector was transformed into S. cerevisiae and was then induced by galactose. The LTB protein was detected in the total soluble protein of the yeast by SDS-PAGE analysis. Quantitative ELISA showed that the maximum amount of LTB protein expressed in the yeast was approximately $1.9\%$ of the total soluble protein. Immunoblotting analysis showed the yeast-derived LTB protein was antigenically indistinguishable from bacterial LTB protein. Since the whole-recombinant yeast has been introduced as a new vaccine formulation the expression of LTB in S. cerevisiae can offer an inexpensive yet effective strategy to protect against ETEC, especially in developing countries where it is needed most.

Cadmium biosorption by a glyphosate-degrading bacterium, a novel biosorbent isolated from pesticide-contaminated agricultural soils

Elnaz Khadivinia,Hakimeh Sharafi,Faranak Hadi,Hossein Shahbani Zahiri,Sima Modiri,Azadeh Tohidi,Amir Mousavi,Ali Hatef Salmanian,Kambiz Akbari Noghabi 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.6

In this study, biosorption of cadmium (II) ions from aqueous solutions by a glyphosate degradingbacterium, Ochrobactrum sp. GDOS, was investigated in batch conditions. The isolate was able to utilize3 mM GP as the sole phosphorous source, favorable to bacterium growth and survival. The effect ofdifferent basic parameters such as initial pH, contact time, initial concentrations of cadmium ion andtemperature on cadmium uptake was evaluated. The adsorption process for Cd (II) is well fitted withLangmuir adsorption isotherm. Experimental data were also tested in terms of biosorption kinetics usingpseudo-first-order and pseudo-second-order kineticmodels. Maximummetal uptake qmax was obtainedas 83.33 mg g1. The sorption process of cadmium onto the Ochrobactrum sp. GDOS biomass followedsecond-order rate kinetic (R2 = 0.9986). A high desorption efficiency was obtained in pH 2. Reusability ofthe biomass was examined under successive biosorption–desorption cycle repeated thrice. Thecharacteristics of the possible interactions between biosorbent and metal ions were also evaluated byscanning electron microscope (SEM), Fourier transform infrared (FT-IR) and X-ray diffraction analysis.