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Saeyoull Cho,이선화,박수진,최규업,조준모,허장현,Anupama Shrestha,임춘근 한국식물병리학회 2009 Plant Pathology Journal Vol.25 No.1
Pseudomonas fluorescens strain Gpf01, isolated from ginseng rhizosphere showed antiviral activity against Cucumber mosaic virus, when tested in a local host of CMV, Chenopodium amaranticolor. Transposon mutant library of Gpf01 was prepared using pGS9::Tn5 and the mutant Gpf01-RS19 was found to loose antiviral production. We developed primers from the flanking region of Tn5 and found a cosmid clone pAV1123, harboring 1.2 kb antiviral compound producing (avcf01) locus. When a sub-clone pPH9, which carried 9.3 kb region of pAV1123, was introduced into antivirus deficient P. fluorescens wild type strain B16, it exhibited antiviral activity. Using Tn3-gus mutagenesis and complementation analysis, it was found that the genes related to antiviral activity production resided in a 9.3- kb HindIII-HindIII fragment of pAV1123, indicating that the plasmid carries an essential genes promoting antiviral activity.
Cho Youngwoo,Cho Saeyoull 한국곤충학회 2021 Entomological Research Vol.51 No.9
There are four types of hemocytes in the stag beetle hemolymph: granulocytes, prohemocytes, coagulocytes, and spherulocytes. Unusually, the size and shape of the hemocyte subtypes are similar. To identify the predominant immune hemocytes, we injected carboxylate-modified polystyrene latex beads and E. coli particles into the hemocoel of stag beetles. In response to the pathogens, only granulocytes underwent morphological changes, including the extension of fan (or amoeba)-like structures from their membrane, and had bacterial particles in their cytoplasm. At 6 h post-injection, lysosomes in the cytoplasm of the granulocytes were activated to remove the pathogens. At 12 h post-injection, more lysosomes were activated along with phagocytosis of the E. coli particles. At 24 h to 48 h post-injection, the bacterial particles, including activated lysosomes, were gradually cleared from the cytoplasm. To confirm these results, we performed flow cytometry analysis. Consistent with our microscope observations, the granulocytes were activated after 6 h post-infection, and lysosomes were mostly activated at 12 h. These results suggest that bacteria are actively engulfed between 6 and 12 h post-infection by granulocytes. Granulocyte lysosomes were activated within 12 h to digest the bacteria, which were completely cleared by 48 h post-infection.
Junmo Cho,Sujin Park,Chunkeun Lim,Yong Chul Park,Jang Hyun Hur,Soonsung Hong,Thomas M. Brown,Saeyoull Cho 한국응용곤충학회 2008 Journal of Asia-Pacific Entomology Vol.11 No.3
Mutations at V421M and L1029H in the hscp sodium channel gene are known to contribute to knockdown resistance (kdr) in the Woodrow, Dalzell, and PTJ strains of H. virescens (tobacco budworm) from the cotton fields of South Carolina, USA. In the IS6 region of the sodium channel gene, the frequencies of the mutant allele methionine in the Woodrow and Dalzell strains were 0.07 and 0.1, respectively. For the IIS6 region, the frequencies of the mutant allele histidine in Woodrow and Dalzell strains were 0.175 and 0.263, respectively. In the PTJ strain, the frequencies of methionine and histidine alleles were 0 and 0.1, respectively. The Hpy3 allele, which is strongly linked to the histidine mutant allele, was also found in Woodrow and Dalzell strains. In addition, we found a new allele, which is one nucleotide different from Hpy3, called Hpy3-1, and found that it is also linked to histidine. Mutations at V421M and L1029H in the hscp sodium channel gene are known to contribute to knockdown resistance (kdr) in the Woodrow, Dalzell, and PTJ strains of H. virescens (tobacco budworm) from the cotton fields of South Carolina, USA. In the IS6 region of the sodium channel gene, the frequencies of the mutant allele methionine in the Woodrow and Dalzell strains were 0.07 and 0.1, respectively. For the IIS6 region, the frequencies of the mutant allele histidine in Woodrow and Dalzell strains were 0.175 and 0.263, respectively. In the PTJ strain, the frequencies of methionine and histidine alleles were 0 and 0.1, respectively. The Hpy3 allele, which is strongly linked to the histidine mutant allele, was also found in Woodrow and Dalzell strains. In addition, we found a new allele, which is one nucleotide different from Hpy3, called Hpy3-1, and found that it is also linked to histidine.
조세열,Cho, Saeyoull 한국응용곤충학회 2016 한국응용곤충학회지 Vol.55 No.3
본 연구는 흰점박이꽃무지(Protaetia brevitarsis seulensis (Colbe) (Cetoniidae, Coleoptera) 유충의 혈림프에 존재하는 혈구세포들의 형태학적 특성분석을 위하여 수행하였다. 흰점박이꽃무지 유충 혈강 내에는 과립혈구세포, 세포질혈구세포, 편도혈구세포, 구상적혈구세포, 전혈구세포, 지방혈구세포 총 여섯 종류의 혈구세포들이 관찰 되었다. 그 중 과립혈구세포는 핵, 미토콘드리아, 골지체를 포함하여 잘 발달된 세포소기관들이 관찰 되었고 외래물질 침입시 면역학적 식균작용을 수행하는 것으로 밝혀졌다. 특히, 과립혈구세포의 세포질에는 잘 발달된 리소좀(<$1{\mu}m$ 직경)들이 세포막 주변으로 분포되어 존재하고 있음을 알 수 있었다. 식균된 외래물질은 다양한 크기의 리소좀들과 서로 합쳐지면서 외래물질을 제거하는 것으로 판단된다. 그 외 다섯 종의 혈구세포들은 외래물질 침입시 면역학적 활성화와 관계가 없는 것으로 관찰되었다. In this study, we used electron microscopic analysis to characterize the hemocytes in the last larva of Protaetia brevitarsis seulensis (Colbe) (Cetoniidae, Coleoptera). Granulocytes (GR), plasmatocytes (PL), oenocytoids (OE), spherulocytes (SP), prohemocytes (PR) and adipohemocytes (AD) were classified based on their size and ultrastructural differences in the circulating hemocytes. Many dark granules (<$1{\mu}m$ in diameter) in the GR's cytoplasm were observed and well-developed mitochondria, endoplasmic reticulum (ER), nucleus, and Golgi complex were also seen. After microorganisms infected, the GRs were morphologically activated and phagocytosed them. Especially, dark granules (lysosomes) were fused themselves and these bigger granules finally agglomerate together with microorganisms. Other hemocytes seem to have no immune functions.