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Lee, Han Saem,Kim, Ji Yun,Lee, Won Il,Kim, Sung Jin,Ko, Min Ji,Jeong, Sunjoo,Park, Keerang,Choe, Han,Lee, Heuiran Spandidos Publications] 2011 Oncology letters Vol.2 No.6
<P>Recombinant adeno-associated virus serotype 5 (rAAV5) is considered to be a promising gene transfer vehicle. However, preferential gene delivery to the tumor remains a requirement for cancer treatment. We generated rAAV5 mutants bearing tumor marker-binding peptides and analyzed their properties as viral vectors, as well as their transduction efficiencies and preferential antitumoral potencies. All of the mutants were successfully produced. Transduction analyses showed that rAAV5 mutants harboring tumor-homing peptides, including RGD and TnC, transduced human cancer cells expressing corresponding receptors on their surfaces. RGDS peptides and TnC antibodies significantly suppressed transduction by rAAV5-RGD and rAAV5-TnC. Cytotoxicity was evident upon transfer of HSV-TK to cells by re-targeted rAAV5. These results provide evidence that rAAV5 vectors, genetically armed with tumor-targeting ligands, preferentially infect human cancer cells harboring the corresponding receptors, thereby inducing antitumoral effects. Further optimization of rAAV5 mutant viruses should thus facilitate practical exploitation of these vectors for gene-based cancer treatment.</P>
Determination of hesperidin in mixed tea by HPLC
Saem Han(한샘),So-Youn Mok(목소연),Hye-Min Kim(김혜민),Jeong-Min Lee(이정민),Dong Gu Lee(이동구),Sook-Young Lee(이숙영),Jongkee Kim(김종기),Sun-Ju Kim(김선주),Sanghyun Lee(이상현) 충남대학교 농업과학연구소 2011 농업과학연구 Vol.38 No.2
The content of hesperidin in the mixed tea, which was composed of dried orange peel, laurel leaf, mulberry leaf, silver magnolia leaf, oriental melon tap, cassia seed, and licorice root, was determined by high performance liquid chromatography (HPLC). Hesperidin was quantified by a reverse phase column with gradient solvent system (watcr:acetonitrile = 80:20 to 35:65 for 30 min) and UV/VIS detection (280 ㎚). The How rate was kept constant at 1.0 ㎖/min. The content of hesperidin in the mixed tea was measured in depending on extraction time 1, 2, 3, and 4 min (29.07, 52.39, 52.45, and 88.35 ㎎/g, respectively).
Lee, Han Saem,Kim, A.-Reum,Kim, Kisoon,Lee, Wan-Ji,Kim, Sung Soon,Kim, You-Jin Elsevier 2017 Protein expression and purification Vol.132 No.-
<P><B>Abstract</B></P> <P>Human respiratory syncytial virus (HRSV) is a main cause of lower respiratory tract infections in infants and the elderly. Glycoprotein (G) is major antigen on the viral surface, and plays a key role for virus entry. Therefore, purification of the glycoprotein of HRSV is critical for the development of HRSV vaccine and serological diagnosis. In this study, we report the design and characterization of glycoprotein engineered rationally to enhance the protein solubility and to facilitate efficient purification. We permuted HRSV glycoproteins with two tags: (i) an immunoglobulin (Ig) M signal peptide and a protein A B domain tag to render HRSV glycoprotein secret into the culture media and (ii) a foldon and 6 × histidine tag with or without transmembrane domain. Three recombinant baculoviruses were constructed: (i) transmembrane-truncated HRSV glycoprotein (amino acid positions 66–298) inserted with the N-terminal IgM signal peptide and protein A B domain (MG-GΔTM), (ii) truncated HRSV glycoprotein (amino acid positions 66–298) fused with a C-terminal foldon and 6 × histidine tag (GΔTM-FH), and (iii) full-length HRSV glycoprotein (amino acid positions 1–298) fused with a C-terminal foldon and 6 × histidine tag (G-FH). Highly soluble recombinant MG-GΔTM protein was clearly purified using one-step affinity chromatography with IgG-sepharose resin, whereas the recombinant G-FH protein and truncated GΔTM-FH were purified partially using nickel-resin. Although, the antigenicity of GΔTM-FH was stronger than highly mannose-rich MG-GΔTM protein, MG-GΔTM induced neutralizing antibodies efficiently in the mice to protect from infectious HRSV.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Three HRSV-G proteins were expressed with different tagging proteins. </LI> <LI> MG tag enhanced the production and the solubility of HRSV-G protein successfully. </LI> <LI> MG-GΔTM is glycosylated abundantly and induces the neutralizing antibodies. </LI> </UL> </P>
Lee, Yeon-Ju,Han, Saem,Lee, Hyi-Seung,Kang, Jong Soon,Yun, Jieun,Sim, Chung J.,Shin, Hee Jae,Lee, Jong Seok American Chemical Society and American Society of 2013 Journal of natural products Vol.76 No.9
<P>Seventeen bromotyrosine-derived metabolites, including eight new compounds, were isolated from a Micronesian sponge of the genus <I>Suberea</I>. Four of the new compounds were psammaplysin derivatives (<B>10</B>–<B>13</B>), and the other four were ceratinamine derivatives (<B>14</B>–<B>17</B>). Of the compounds obtained, the psammaplysins exhibited cytotoxicity against human cancer cell lines (GI<SUB>50</SUB> values down to 0.8 μM), while the ceratinamine and moloka’iamine analogues showed almost no activity. These results suggest that the spirooxepinisoxazoline ring system is a requirement for cytotoxicity and, therefore, may serve as an attractive molecular scaffold for the development of a potent anticancer agent.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jnprdf/2013/jnprdf.2013.76.issue-9/np400448y/production/images/medium/np-2013-00448y_0005.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/np400448y'>ACS Electronic Supporting Info</A></P>
Bioconverted Orostachys japonicas Extracts Suppress Angiogenic Activity of Ms-1 Endothelial Cells
Lee, Seul Gi,Kim, Jin Soo,Lee, Han-Saem,Lim, Yu-Mi,So, Jai-Hyun,Hahn, Dongyup,Ha, Yu Shin,Nam, Ju-Ock Molecular Diversity Preservation International 2017 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.18 No.12
<P>Orostachys japonicus A. Berger (), known as Wa-song in Korea, has been reported to exert various biological effects, such as anti-tumor, anti-oxidant, and anti-febrile effects. However, the anti-angiogenic effects of O. japonicus extracts remain to be investigated. In the present study, we demonstrated the anti-angiogenic effects of bioconverted O. japonicus extract (BOE) in Ms-1 mouse endothelial cells and compared them with the bioactivities of O. japonicus extract (OE). BOE, but not OE, were found to exert anti-angiogenic effects, including inhibition of cell migration, cell adhesion, tube formation of Ms-1 cells, and blood vessel formation of matrigel plug assay in vivo. Furthermore, protein levels of phosphorylated Src kinase were lower in BOE-treated cells than in OE-treated cells. Treatment with OE or BOE did not influence cell viability during the experimental period. Bioconverted extract of O. japonicus have anti-angiogenic effects in vitro and vivo, but non-bioconverted extract do not. We suggest that these observed anti-angiogenic effects are caused by the changes in the composition of bioactive compounds in the extracts as a result of biological conversion.</P>
Severe adenovirus pneumonia in young military man: a case report
( Saem Na Lee ),( Ji Yun Noh ),( Min Joo Choi ),( Jin Gu Yoon ),( Ji Ho Jeon ),( Eun Ju Jung ),( Seonghui Kang ),( Joon Young Song ),( Hee Jin Cheong ),( Woo Joo Kim ) 대한내과학회 2015 대한내과학회 추계학술대회 Vol.2015 No.1
Human adenoviruses (HAdVs) is a common cause of acute upper respiratory tract infection in adults, but it rarely causes respiratory failure or septic shock, severe and life-threatening infection. Although severe adenovirus pneumonia is well described in neonates, young children, and immunocompromised hosts, it is relatively unusual in healthy adults. Outbreaks of HAdV pneumonia have been described in military and long term care settings. Herein, we report an adenovirus pneumonia case in a previously healthy young military man referred from a military hospital, who needed a care in ICU. A 19-year-old healthy male patient who was in a military training session referred from military hospital due to fever with aggravated respiratory symptoms. His initial chest X ray showed air-space consolidation and centrilobular densities were on both lungs, mainly RLL. Comparing the previous chest images taken in military hospital, his pneumonia was aggravated in only a few days. Ceftriaxone and levofloxacin were given to the patient.. On hospital day 3, the result of respiratory virus PCR was reported, and all other respiratory virus were negative except adenovirus. Though antibiotics administered continuously to cover concomitant bacterial infection, there was no evidence of any bacterial infection in all respiratory specimen including broncho-alveloar lavage fluid and induced sputum. His symptom and pneumonic infiltration became with supportive cares, finally he discharged without any respiratory sequelae. In a military, among the young soldiers, outbreak of adenovirus has been reported. This case emphasizes that surveillance for respiratory illness is required in a military, because adenovirus infection can presented with severe pneumonia especially among military trainees.