오리 鼻腔 및 鼻粘膜上皮의 計測에 관한 硏究

金世仁,金武剛,申台均,金弘善,柳時潤,李永浩 충남대학교 의과대학 지역사회의학연구소 1986 충남의대잡지 Vol.13 No.1

The area of the various epithelial types linging the nasal navity of each six number in 1-week-old and 10-week-old adult ducks has been made for the light microscopic research. Transverse sections of 10 microns thick in each specimen were made continuously from the anterior end of nostril to the posterior end of pharyngeal inlet and specimen samples were selected at 0.13mm(1-week-old) and 0.17mm(10-week-old) intervals from the anterior end of the nasal cavity to the posterior end of pharyngeal inlet. The results obtained from research were summarized as follows. 1. The nasal cavity is lined with stratified squamous, respiratory and olfactory epithelium. The thickness of stratified squamous, respiratory and olfactory epithelium is 10-100㎛, 8-75㎛ and 50㎛ respectively in 1-week-old ducks, and 25-175㎛, 20-125㎛ and 66㎛ respectively in 10-week-old ducks. 2. The unilateral volumes of the nasal cavity in 1-week-old and 10-week-old ducks were 21.73±2.64㎟, 1,692.44±171.57㎟ respectively. 3. The total unilateral surface areas of the nasal cavity in 1-week-old and 10-week-old ducks were 70.44±4.92㎟, 1,772.45±94.10㎟ respectively. 4. Each area of the stratified squamous epithelium, respiratory epithelium and olfactory epithelium in unilateral nasal cavity is 14.82±1.85㎟ (21.1%), 46.86±3.20㎟ (66.5%) and 8.76±0.40㎟ (12.4%) in the 1-week-old ducks, and 348.23±40.02㎟ (19.7%), 1,282.31±47.03㎟ (72.3%) and 141.90±12.43㎟ (8.0%) in the 10-week-old ducks. Ducks are assumed as a microsmatic animals by the composition of the lining epithelium of the nasal cavity in ducks.

단기간 크레아틴 섭취가 태권도 선수의 신체조성, 등속성 근력 및 혈액성분에 미치는 영향

오세용,김창은,김의영 한국스포츠리서치 2003 한국 스포츠 리서치 Vol.20 No.1

The purpose of this study was to examine the effect of short-term creatine intake on constitution, isokinetic muscular power and constituent parts of the blood among taekwondo players. After 14 taekwondo players were divided into two groups, one group took creatine for six days and the other took placebo for the same short period of time. This experiment was conducted during training period, and then their constitution, isokinetic muscular power, blood creatinine and inorganic phosphate were measured. The findings of this study were as follows: 1. As for constitution, there was no significant disparity in weight, the amount of muscle, body fat volume and body fat rate between the creatine group and the placebo group. 2. Concerning isokinetic muscular power, the creatine group made a significant progress in set total work(J) and endurance rate(%) after the two groups were engaged in maximal flexion/extension exercise(180˚/s) 30 times. 3. When the constituent parts of the blood was gauged, the creatine group showed a significant increase in creatinine at rest and 30 minutes after recovery(p<.05), and the placebo group showed a significant reduction in creatinine at rest and immediately after workout(p<.05). In the former group, there was no change in inorganic phosphate, but the placebo group showed a significant increase at rest, immediately after workout and 30 minutes after recovery(p<.05). The above-mentioned findings suggested that the six-day creatine intake had an effect on the muscular power of the taekwondo players and their constituent parts of the blood, but it gave no positive impact on their physical constitution. Therefore, short-term creatine intake seemed to contribute to enhancing motor skills.

Gilbert 증후군에서 열량 제한 시험과 Phenobarbital 자극 시험의 의의(14예)

이헌영,채경훈,정재훈,강윤세,김연수,문희석,박기오,이엄석,김선문,김석현,성재규,이병석,이강욱 충남대학교 의학연구소 2003 충남의대잡지 Vol.30 No.2

Gilbert 증후군은 인구의 7%에서까지 나타날 수 있는 매우 흔한 증후군으로서 비진행성인 양성의 만성적 경과를 치하며, 간질환의 증상과 징후가 없는 경한 비포합형 고빌리루빈혈증이 특징인 일종의 체질적인 증상으로서 혈장 빌리루빈 농도에 대한 사춘기의 영향 때문에 10대와 20대에 자주 진단이 된다. 따라서 임상적인 중요성은 미약하지만 높은 빈도가 예상되는 점에 그 중요성이 부여되어야 할 것이다. 따라서 적정한 임상적 진단법으로 기왕에 소개된 열량제한 시험과 phenobarbital 유도 시험을 시행하고 이들의 진단적 가치를 알아보기 위하여 본 연구를 시행하였다. 1990년 7월부터 1999년 4월까지 충남대학교병원에 내원하여 HBsAg, IgG anti-HBc 및 anti-HCV가 음성이고, 간 초음파 스캔에서 이상이 없으며, 혈청 AST, ALT 및 AP가 정상인 비음주자에서 경한 비포합형 고빌리루빈혈증이 있는 14예의 환자들을 대상으로 ^(99m)Tc-DISID 스캔을 시행하였으며, 기저 치 총빌리루빈 및 포합형 빌리루빈 치를 측정한 다음에 하루에 400Kcal로 48시간동안 제한한 열량 제한 시험을 시행하였고, phenobarbital을 하루 60mg씩 5일간 투여한 후에도 각각 총빌리루빈과 포합형 빌리루빈 치를 검사하여 비포합형을 구하였다. 대상 환자들은 모두 14예로서 남자가 11예(78.6%)였고 여자가 3예(21.4%)여서 3.7:1로 남자에서 많았으며, 20대가 6예(42.9%), 30대가 역시 6예(42.9%) 및 40대가 2예(14.2%)로서 2,30대가 대부분(85.8%)이었다. 열량 제한 시험 후의 총빌리루빈 치, 비포합형 및 포합형 빌리루빈 치들은 평균 각각 5.5±2.7, 4.2±2.3 및 1.3±10mg/dL 로서, 시험 전 치들인 3.0±0.8, 2.2±0.8 및 0.7±0.4mg/dL 보다 유의하게(p=0.001, p=0.001, p=0.023) 상승하였다. 포합형 빌리루빈 치도 유의하게 상승하였으나 비포합형의 상승보다는 훨씬 낮아서 주로 비포합형이 증가하였다. phenobarbital 투여 중 설사가 발생하여 중단한 1예를 제외한 13예에서 열량 제한 시험 후에 상승하였던 총, 비포합형 및 포합형 빌리루빈 치가 phenobarbital 유도 시험후에는 2.0±1.1, 1.5±0.8 및 0.5±0.4mg/dL로서 열량 제한 시험 결과보다 유의하게 낮아졌고(p=0.00, p=0.000, p=0.001), 열량 제한 시험 전의 기초치들인 3.0±0.8, 2.2±0.8 및 0.7±0.4mg.dL 보다도 더욱 낮아졌으며 유의한 차이(p=0.001, p=0.02, p=0.005)를 나타내었다. 14예에서 시행한 ^(99m)-Tc DISIDA 스캔에서 9예(64.3%)가 정상이었고, 5예(35.7%)에서는 심장 및 신장으로의 간외 섭취가 3예였고, 60분까지 소장 배출이 없는 배설 지연 예와 담낭 수축 불량 예가 각각 1예 씩 발견되었다. Phenobarbital 투여시험에서 민감도가 열량제한시험에 비해 더 높았다(92.3%와 50.0%). Gilbert 증후군에서 1일 400 Kcal로 48시간의 열량제한 시험과 1일 60mg의 phenobarbital을 5일간 투여하는 유도 시험은 편리하고 유용한 임상적인 진단법으로 이용할 수 있다고 생각된다. 그러나 열량 제한 시험에서는 증가 기준의 통일이 필요하다고 유추되며 phenobarbital 유도 시험이 민감도가 더 높은 것으로 생각된다. Gilbert's syndrome is very frequent and benign chronic process characterized by mild, intermittent, unconjugated hyperbilirubinemia without any symptom and sign of liver disease. Previously intoduced caloric restriction test and phenobarbital stimulation test as two appropriate clinical tests had been examined and their diagnostic values were reevaluated. Fourteen patients with mild, persistent, unconjugated hyperbilirubinemia were included. Subsequently caloric restriction has been applicated by 400 Kcal/day for 48 hours and phenobarbital has been prescribed by 60 mg/day for 5 days. Therafter serum levels of total and direct bilirubin were measured. Most of the patients were third and fourth decade(85.8%) and male predominant. Each basal serum levels of total, indirect and direct bilirubin were 3.0±0.8, 2.2±0.8 and 0.7±0.4 mg/dL. After caloric restriction test, each levels were increased significantly to 5.5±2.7, 4.2±2.3 and 1.3±1.0 mg/dL(p=0.001, p=0.001, p=0.023). After phenobarbital stimulation test for 13 patients had been practiced, increased levels of each bilirubin after caloric restriction test were decreased significantly to 2.0±1.0, 1.5±0.8 and 0.5±0.4 mg/dL(p=0.000, p=0.000, p=0.001) and these levels were significantly lower than basal levels(p=0.001, p=0.02, p=0.005). The sensitivities of caloric restriction test were 85.7%, 50.0%, and 71.4%, 35.7%(1.0, 1.5 mg increase of total bilirubin and 1.0, 1.5 mg/dL increase of indirect bilirubin). The sensitivities of phenobarbital stimulation test were 93.2% and 92.3% at criteria of 1.5 mg/dL increase of total bilirubin and indirect bilirubin. On the diagnosis of Gilbert syndrome, caloric restriction test and phenobarbital stimulation test are convenient and useful diagnostic tools in clinical face. And also phenobarbital stimulation test has higher sensitivity than caloric restriction test. Furthermore, standardization of bilirubin increment would be necessary in caloric restriction test.

발생원별에 따른 PAHs 배출특성

박찬구,윤중섭,김민영,손종열,모세영 한국대기환경학회 2004 한국대기환경학회지 Vol.20 No.3

The results of individual PAH source profiles that can be applied to receptor model are as follows. The sum of 16 PAH concentrations was 391.41 ng/S㎥ in a tunnel. Phenanthrene was the most abundant compound among 16 PAH, and then pyrene, fIuoranthene, anthracene, and naphthalene can be seen in elevated contents. 11,056.61 ng/S㎥ of 16 PAH concentrations in BC oil boiler was two times higher than 6,582.57 ng/S㎥ of those in LNG boiler. Naphthalene was the most abundant compound in both facilities. Phenanthrene, anthracene. and acenaphthylene were the second dominant compound group in order from both facilities. BC oil boiler had relatively high concentration of pyrene compared to LNG boiler that had high concentration of fluorene and did not detect pyrene. The sum of 16 PAH concentrations emitted from MSW incinerators after APCD (air pollution control device) was three times higher than those from MSW incinerators bcfore APCD. However, the concentrations of more than 4-ring PAH compounds (e.g., benzo (a)anthracene) before APCD were higher than those after APCD. This fact implies that PAHs generated by combustion process are eliminated in APCD and they are continuously produced in stack or atmosphere by PAHs precursors.

High-frequency micromechanical resonators from aluminium–carbon nanotube nanolaminates

Bak, Jung Hoon,Kim, Young Duck,Hong, Seung Sae,Lee, Byung Yang,Lee, Seung Ran,Jang, Jae Hyuck,Kim, Miyoung,Char, Kookrin,Hong, Seunghun,Park, Yun Daniel Nature Publishing Group 2008 NATURE MATERIALS Vol.7 No.6

At micro- and nanoscales, materials with high Young’s moduli and low densities are of great interest for high-frequency micromechanical resonator devices. Incorporating carbon nanotubes (CNTs), with their unmatched properties, has added functionality to many man-made composites. We report on the fabrication of ≤100-nm-thick laminates by sputter-deposition of aluminium onto a two-dimensional single-walled CNT network. These nanolaminates—composed of Al, its native oxide Al<SUB>2</SUB>O<SUB>3</SUB> and CNTs—are fashioned, in a scalable manner, into suspended doubly clamped micromechanical beams. Dynamic flexural measurements show marked increases in resonant frequencies for nanolaminates with Al–CNT laminae. Such increases, further supported by quasi-static flexural measurements, are partly attributable to enhancements in elastic properties arising from the addition of CNTs. As a consequence, these nanolaminate micromechanical resonators show significant suppression of mechanical nonlinearity and enhanced strength, both of which are advantageous for practical applications and analogous to biological nanocomposites, similarly composed of high-aspect-ratio, mechanically superior mineral platelets in a soft protein matrix.

Effects of chronic alcohol consumption on expression Levels of APP and Aβ-producing enzymes

( Sae Rom Kim ),( Hye Young Jeong ),( Sung Hee Yang ),( Sung Pil Choi ),( Min Young Seo ),( Young Kwang Yun ),( Yuri Choi ),( Sang Ha Baik ),( Jong Sung Park ),( A Ryeong Gwon ),( Dong Kwon Yang ),( C 생화학분자생물학회(구 한국생화학분자생물학회) 2011 BMB Reports Vol.44 No.2

Chronic alcohol consumption contributes to numerous diseases, including cancers, cardiovascular diseases, and liver cirrhosis. Epidemiological studies have shown that excessive alcohol consumption is a risk factor for dementia. Along this line, Alzheimer`s disease (AD) is the most common form of dementia and is caused by the accumulation of amyloid-β (Aβ plaques in neurons. In this study, we hypothesized that chronic ethanol consumption is associated with pathological processing of APP in AD. To investigate the relationship between chronic alcohol consumption and Aβ production, brain samples from rats fed an alcohol liquid diet for 5 weeks were analyzed. We show that the expression levels of APP, BACE1, and immature nicastrin were increased in the cerebellum, hippocampus, and striatum of the alcohol-fed group compared to the control group. Total nicastrin and PS1 levels were induced in the hippocampus of alcohol-fed rats. These data suggest that the altered expression of APP and Aβ-producing enzymes possibly contributes to the chronic alcohol consumption-mediated pathogenesis of AD. [BMB reports 2011; 44(2): 135-139]

Primary Cultures of Drosophila melanogaster Gut Cells for Studies of Intestinal Stem Cell Regulation

Young-Il Yoon(윤영일),Jae-Sam Hwang(황재삼),Tae-Won Goo(구태원),Myung-Sae Han(한명세),Mi-Young Ahn(안미영),Eun-Young Yun(윤은영) 한국생명과학회 2012 생명과학회지 Vol.22 No.5

초파리는 발생과 질병연구를 위한 모델 곤충으로 널리 이용되어 왔다. 본 연구에서도 초파리를 모델곤충으로 한 장질환 연구의 일환으로 다양한 병원균 감염 및 장질환 유발시 어떻게 장줄기세포가 작용하는지를 이해하기 위해 초파리 장세포의 일차배양 방법을 확립하였다. 초파리 성충으로부터 장을 해부하고 다양한 효소를 처리하여 장세포를 분리한 후 배양하였다. 배양세포의 생존여부는 현미경 검경 및 MTS assay에 의해 확인한 결과 배양 후 9일째 최대 증식되었고 14일까지 생존함을 확인할 수 있었다. 또한 장줄기세포 및 장내분비세포의 존재도 immunostaining에 의해 확인하였다. 따라서 본 연구에서 구축된 초파리 일차배양 장세포는 다양한 유전자에 의한 장줄기 세포 조절연구뿐만 아니라 장에서 발생하는 다양한 질병을 연구하는 도구로 매우 유용할 것으로 추측된다. Drosophila melanogaster has been used as a useful model to study development and disease. In this study, we established the primary culture method of Drosophila in the intestine to understand how intestinal stem cells (ISCs) mediate tissue repair during infection and disease. To obtain intestinal cells, we separated intestines from adult flies and isolated single cells by enzymatic treatment. The survival of cultured cells was measured using MTS-analysis. The maximum growth rate of the cells was observed on the 9th day after seeding. In addition, the presence of ISCs and enteroendocrine cells was confirmed by delta and prospero staining. Accordingly, we supposed that Drosophila melanogaster gut cells established in this study are probably useful in studies about intestinal stem cell regulation and various diseases occurring in the intestine.

Effects of Anodized Titanium Implant Coated with RGD Peptides via Chemical Fixation on Osseointegration and Bone Regeneration

( Sae Young Park ),( Hyo Sop Kim ),( Jae Ho Kim ),( Jin Hyung Shim ),( Mi Jung Yun ),( Young Chan Jeon ),( Jung Bo Huh ),( Chang Mo Jeong ) 한국조직공학·재생의학회 2012 조직공학과 재생의학 Vol.9 No.4

This study was conducted to investigate the effects of an anodized titanium surface with RGD-peptides. Silane was used to immobilize the RGD peptides to the titanium surfaces. The surfaces were examined by scanning electron microscope (SEM) and X-ray photoelectron spectroscopy (XPS). Crystal violet assay, XTT assay and Alkaline phosphatase (ALP) assay were performed to evaluate response of human mesenchymal stem cell. For in vivo study, twenty-four implants and four beagle dogs were used and made the dehiscence defect model (n=12 in each group). Implant stability quotient (ISQ) value was measured and evaluated by histometric analysis. Histological examination revealed an increase of bone height in the test group. There were significant differences in intra-thread bone density (ITBD) between the groups. The application of RGD peptide to the implant surface may contribute to the improvement of osseointegration quality and bone growth in a small defect area around the implant.

Mirror Therapy for Phantom Limb Pain

( Sae Young Kim ),( Yun Young Kim ) 대한통증학회 2012 The Korean Journal of Pain Vol.25 No.4

Phantom limb pain is a painful sensation that is perceived in a body part that no longer exists. To control this pain, many methods have been used such as medication, physical treatment, nerve block, neuromodulation, surgical treatment and mirror therapy. However, until now, there effects have been uncertain. We report the successful reduction of phantom limb pain using mirror therapy when other treatments initially failed to control the pain.

Nucleophile-dependent regioselective ring opening of 2-substituted <i>N</i>,<i>N</i>-dibenzylaziridinium ions: bromide <i>versus</i> hydride

Yun, Sae Young,Catak, Saron,Lee, Won Koo,D’hooghe, Matthias,De Kimpe, Norbert,Van Speybroeck, Veronique,Waroquier, Michel,Kim, Yongeun,Ha, Hyun-Joon Royal Society of Chemistry 2009 Chemical communications Vol.2009 No.18

<P>The ring opening of 2-substituted <I>N</I>,<I>N</I>-dibenzylaziridinium ions by bromide exclusively occurs at the substituted aziridine carbon atom in a stereospecific way, whereas the opposite regioselectivity was observed for hydride-induced ring opening at the unsubstituted position; furthermore, this unprecedented hydride-promoted reactivity was validated by means of Density Functional Theory (DFT) calculations.</P> <P>Graphic Abstract</P><P>The ring opening of 2-substituted <I>N</I>,<I>N</I>-dibenzylaziridinium ions by bromide exclusively occurs at the substituted aziridine carbon atom in a stereospecific way, whereas the opposite regioselectivity was observed for hydride-induced ring opening at the unsubstituted position. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=b822763b'> </P>