Effects of $BaCO_3$ purity on the superconducting properties of top seeded melt growth processed $Y_{1+x}Ba_2Cu_3O_y$ superconductors

Choi, J.S.,Park, S.D.,Jun, B.H.,Han, Y.H.,Sung, T.H.,Choo, K.N.,Kim, C.J. The Korean Society of Superconductivity and Cryoge 2009 한국초전도저온공학회논문지 Vol.11 No.2

Effects of $BaCO_3$ purity on the superconducting properties of top seeded melt growth (TSMG) processed $Y_{1+x}Ba_2Cu_3O_{7-y}$ (Y1+x, x=0.1 and 0.2) superconductors were investigated. $YBa_2Cu_3O_{7-y}$ (Y123) powder prepared using $BaCO_3$ with 99.75% purity and commercially available Y123 powder of 99.9% were used for the fabrication of single Y123 grain superconductors. $T_c$ values of the Y1+x samples prepared using low purity Y123 powder were slightly lower than those of the samples prepared using a high purity powder. In addition to the lower $T_c$, an anomalous peak effect in the intermediate magnetic fields was observed in Y1+x samples prepared using the low purity $BaCO_3$ powder. The slight decrease in $T_c$ and the anomalous peak effect are ascribed to the possible incorporation of a Y123 phase with impurity elements such as strontium and calcium included in the $BaCO_3$powder of 99.7%. The result suggests that the low purity $BaCO_3$ powder of a low price can be used as a raw power for the fabrication of single grain YBCO bulk superconductors.

Comparison of contractile mechanisms of sphingosylphosphorylcholine and sphingosine-1-phosphate in rabbit coronary artery

Choi, S.-K.,Ahn, D.-S.,Lee, Y.-H. Oxford University Press 2009 Cardiovascular research Vol.82 No.2

<P>AIMS: Although stimulation with sphingosylphosphorylcholine (SPC) or sphingosine-1-phosphate (S1P) generally leads to similar vascular responses, the contractile patterns and their underlying signalling mechanisms are often distinct. We investigated the different reliance upon Ca2+-dependent and Ca2+-sensitizing mechanisms of constriction in response to SPC or S1P in coronary arteries. METHODS AND RESULTS: Contractile responses, changes in [Ca2+]i, and phosphorylation of myosin light chain phosphatase-targeting subunit (MYPT1) were measured. SPC induced a concentration-dependent sustained contraction. S1P evoked a rapid rise in force (initial transient), which was followed by a secondary sustained force. In the absence of extracellular Ca2+, the concentration dependency of constriction to SPC was shifted to the right, but with no change in maximum force, whereas S1P-induced contraction was significantly blunted. Cyclopiazonic acid (CPA) significantly decreased the initial transient force induced by S1P. In isolated single cells, S1P markedly increased [Ca2+]i, whereas only a modest elevation was noted with SPC. The S1P-induced elevation of [Ca2+]i was abolished by pre-treatment with CPA and was significantly reduced in the absence of extracellular Ca2+. In beta-escin-permeabilized strips, SPC augmented pCa 6.3-induced force; this was significantly inhibited by fasudil hydrochloride. S1P induced little or no augmentation of pCa 6.3-induced force. In intact arteries, SPC-induced contraction was completely inhibited by fasudil hydrochloride. Fasudil hydrochloride had no effect on the initial transient force induced by S1P but significantly inhibited the secondary sustained force. SPC induced a several-fold increase in Thr696 and Thr853 phosphorylation of MYPT1, but S1P did not affect phosphorylation of MYPT1. CONCLUSION: Our results suggest that constriction of coronary arteries in response to the bioactive lipid S1P or SPC occurs by distinct signalling pathways. Activation of the RhoA/RhoA-associated kinase pathway and subsequent phosphorylation of MYPT1 play a key role in SPC-induced coronary contraction, whereas elevation of [Ca2+]i is crucial for S1P-induced coronary constriction.</P>

Diagnostic usefulness of a T cell-based assay for latent tuberculosis infection in kidney transplant candidates before transplantation

Kim, S.-H.,Lee, S.-O.,Park, I.-A.,Park, S.J.,Choi, S.-H.,Kim, Y.S.,Woo, J.H.,Park, S.-K.,Park, J.S.,Kim, S.C.,Han, D.J. Blackwell Publishing Inc 2010 Transplant infectious disease Vol.12 No.2

<P>S.-H. Kim, S.-O. Lee, I.-A. Park, S.J. Park, S.-H. Choi, Y.S. Kim, J.H. Woo, S.-K. Park, J.S. Park, S.C. Kim, D.J. Han. Diagnostic usefulness of a T cell-based assay for latent tuberculosis infection in kidney transplant candidates before transplantation.Transpl Infect Dis 2010: <B>12:</B> 113–119. All rights reserved</P><P>Background</P><P>The presence of latent tuberculosis (TB) infection (LTBI) should be evaluated before kidney transplantation. Although a new T cell-based assay for diagnosing LTBI gave promising results, this assay has not yet been compared with the tuberculin skin test (TST) for diagnosing LTBI in renal transplant candidates before transplantation.</P><P>Patients and methods</P><P>All adult patients admitted to a single institute for renal transplantation over a 1-year period were prospectively enrolled. A clinically predictive risk of LTBI was defined as: (i) recent close contact with a person with pulmonary TB; (ii) abnormal chest radiography; (iii) a history of untreated or inadequately treated TB; or (iv) a new infection (i.e., a recent conversion of TST).</P><P>Results</P><P>Of 209 renal recipients, 47 (22%) had a positive TST≥5 mm, 21 (10%) had a positive TST≥10 mm, 65 (30%) had a positive T-SPOT.<I>TB</I> test, and 25 (12%) had an indeterminate T-SPOT.<I>TB</I> test. The induration size of TST was significantly associated with a high positivity rate on T-SPOT.<I>TB</I> (<I>P</I><0.001). Agreement between T-SPOT.<I>TB</I> test and TST≥10 mm was fair (<I>k</I>=0.24, 95% confidence interval 0.11–0.36). However, neither univariate nor multivariate analysis showed any association between the clinical risk for LTBI and positivity on T-SPOT.<I>TB</I> or TST.</P><P>Conclusion</P><P>T-SPOT.<I>TB</I> test was more frequently positive than TST in renal transplant candidates. However, further longitudinal studies are awaited to determine whether the ability of T-SPOT.<I>TB</I> assay to detect LTBI in renal transplant recipients can better predict the development of TB than can TST after transplantation.</P>

Serum lactoferrin level as a serologic biomarker for allergic rhinitis

Choi, G. -S.,Shin, S. -Y.,Kim, J. -H.,Lee, H. -Y.,Palikhe, N. S.,Ye, Y. -M.,Kim, S. -H.,Park, H. -S. Blackwell Publishing Ltd 2010 Clinical and experimental allergy Vol.40 No.3

<P>Summary</P><P>Background</P><P>Allergic rhinitis (AR) is a very common disease and a risk factor for allergic asthma. The discovery of new biomarkers for the early detection of AR would improve the clinical outcomes and reduce socio-economic burden. We sought to identify a novel serologic marker for detection of AR using a proteomic approach.</P><P>Methods</P><P>To identify the proteins involved in AR, comparative proteomics was applied using nasal lavage fluids (NLFs) taken before and after a nasal provocation test (NPT) with <I>Dermatophagoides pteronyssinus</I> (<I>Dpt</I>) in a subject with AR sensitized to <I>Dpt</I>. The clinical relevance of the identified proteins was evaluated by ELISA using NLFs and sera from the three study groups: <I>Dpt</I>-sensitive AR; asymptomatic <I>Dpt</I>-sensitive controls; and non-atopic healthy controls. The sensitivities and specificities of the candidate proteins for predicting AR were determined using receiver operating characteristic (ROC) curves.</P><P>Results</P><P>In proteomic analysis, lactoferrin expression was up-regulated after NPT. The validation study using ELISA showed a significantly lower serum lactoferrin level in the AR group than those of the other two groups (<I>P</I><0.05, respectively). To discriminate between subjects with or without AR, the optimal serum cut-off level of lactoferrin was set at <307 ng/mL using the ROC curve. The sensitivity and specificity for predicting AR were 81.4% and 58%. When combined with serum <I>Dpt</I>-specific IgE level, the sensitivity and specificity for predicting AR were 76.7% and 79.2%.</P><P>Conclusion</P><P>These results suggest that the serum lactoferrin level is associated with the phenotype of <I>Dpt</I>-sensitive AR, and in combination with the serum <I>Dpt</I>-specific IgE level, may be a potential serologic marker for early detection of AR.</P><P><I>Cite this as</I>: G.-S. Choi, S.-Y. Shin, J.-H. Kim, H.-Y. Lee, N. S. Palikhe, Y.-M. Ye, S.-H. Kim and H.-S. Park, <I>Clinical & Experimental Allergy</I>, 2010 (40) 403–410.</P>

딥러닝 기법을 이용한 전해가공의 홀가공 특성 분석

최승건(S. G. Choi),김준영(J. Y. Kim),이은상(E. S. Lee) 한국생산제조학회 2019 한국생산제조시스템학회 학술발표대회 논문집 Vol.2019 No.5

Reactivities of organic isothiocyanates and thiocyanates toward dialkyl bis(phosphine) complexes of palladium(II) and platinum(II)

Lee, S.G.,Choi, K.Y.,Kim, Y.J.,Park, S.,Lee, S.W. Pergamon Press 2015 Polyhedron Vol.85 No.-

Room-temperature reactions of trans-[PdEt<SUB>2</SUB>L<SUB>2</SUB>] (L=PMe<SUB>3</SUB>, PEt<SUB>3</SUB>, PMe<SUB>2</SUB>Ph) with organic isothiocyanates [R-NCS; R=benzyl; CH(CH<SUB>3</SUB>)Ph, R-(-) and S-(+); indanyl, S-(+)] afforded the S,S-coordinated Pd(II) complexes [Pd(S<SUB>2</SUB>C?N-R)L<SUB>2</SUB>] containing a dithiocarbonimidato (S<SUB>2</SUB>C?N-R) group. Similar reactions involving allyl isothiocyanates produced the cationic η<SUP>3</SUP>-allyl Pd complex [Pd(η<SUP>3</SUP>-allyl)(PMe<SUB>3</SUB>)<SUB>2</SUB>]<SUP>+</SUP>(NCS)<SUP>-</SUP>. When [Pd(S<SUB>2</SUB>C?N-R)(PMe<SUB>3</SUB>)<SUB>2</SUB>] was treated with 1equiv of a chelating phosphine [L-L=depe (1,2-bis(diethylphosphino)ethane) and dmpe (1,2-bis(dimethylphosphino)ethane)], the corresponding complexes [Pd(S<SUB>2</SUB>C?N-R)(L-L)] were produced. Reactions of trans-[PdEt<SUB>2</SUB>L<SUB>2</SUB>] (L=PMe<SUB>3</SUB>, PMe<SUB>2</SUB>Ph) with organic thiocyanates (R-SCN; R=benzyl, Et) resulted in the formation of [Pd(CN)<SUB>2</SUB>L<SUB>2</SUB>] and an organic disulfide by S-C bond cleavage of R-SCN. However, similar reactions of the dimethyl analogs, trans-[PdMe<SUB>2</SUB>L<SUB>2</SUB>] (L=PMe<SUB>3</SUB>, PEt<SUB>3</SUB>), with benzyl thiocyanate afforded different products, [Pd(NCS)<SUB>2</SUB>L<SUB>2</SUB>] or [PdMe(NCS)L<SUB>2</SUB>]. Treating [Pt(styrene)(PMe<SUB>3</SUB>)<SUB>2</SUB>] with benzyl isothiocyanate gave the S-coordinated dithiocarbonimidato Pt(II) complex, [Pt(S<SUB>2</SUB>C?N-R)(Me<SUB>3</SUB>P)<SUB>2</SUB>] (R=benzyl). In contrast, cis-[PtEt<SUB>2</SUB>(PMe<SUB>3</SUB>)<SUB>2</SUB>] reacted with the isothiocyanate to afford the trialkyl Pt(IV) complex [PtEt<SUB>2</SUB>(SCN)(CH<SUB>2</SUB>Ph)(PMe<SUB>3</SUB>)<SUB>2</SUB>].

韓國人의 標準體重値와 正常適應體重値에 關한 硏究

尹太永,崔重明,朴淳永,金大棒 韓國保健敎育學會 1996 보건교육건강증진학회지 Vol.13 No.1

Using Random Sampling, the authors measured the body heights and weights of 31,151 persons- 17,102 in males and 14,049 in females from metropolitan, urban and rural areas between 6 to over 80 year old - for the purpose of investigating the type and the actual condition of the Korean's growth and development. At first, on the basis of the results, the authors measured the growth and development, various kinds of physiques, nutritional index of the 6 to 20s age group. Second, the authors presented the standard body weight of males and females by their body height, who were in the end of their growth (20-29 age group). Third, the authors calculated and presented the normal adapted body weight of the age group who were over 30 age after the growth had been completed. Forth, the author presented the obesity rate of the adults over 20 years old by body mass index. Finally, the authors compared chronological change of the Koreans' body heights and body weights with the results of other researchers. 1. Body Measurement Rapid growth, in terms of body height, which is described by a straight line on a growth curve has been observed among males in the ages 6-13 and among females 6-14. That growth curve turned out to be slower among the people of higher ages by both sexes. The cross-over occurred in both sexes at 11-14. The highest growth rate for a year is at 13-16 for males and 11-13 for females. This indicates that females enter a rapidly growing stage 2 years earlier than males. 2. Various Physiques and Nutritional Index Rapid growth, in terms of Relative Body Weight Index, which is described by a straight line, has been observed among males in the ages 6-16 and females in the ages 6-14. The cross-over occurred in both sexes 12.5-14.5 age in the adolescencent period. Whereupon females outgrow males. The Roher Index displayed more good value in case of females than male and in the adolescent period, the level of fullness is lower than after the completion of development. The Kaup Indices of both sexes increase with age. The index is less than 2.0 for males in 6-14 age group and for females in 6-13 age group and with this, it appeared that development of horizontal axis to long axis is poor. The index is more than 2.0 after 15 age group in males and 14 age group in females and developmental state4 age group and for females in 6-13 age group and with this, it appeared that development of horizontal axis to long axis is poor. The index is more than 2.0 after 15 age group in males and 14 age group in females and developmental state. Body Mass Index is less than 20 for males 6-14 age group and for females in 6-13 age group. In the case of the higher age group, that index maintains a normal state. 3. Average Body Height, Body Weight and Desirable Body Weight of Korean Youth (20-29 Age Group) The average body weight and body height of full-grown Korean youth was 172.5 ± 5.4㎝ and 66.3 = 9.5㎏ for male, 159.3 ±4.6 ㎝ and 53.5 ±6.9 ㎏ for females. In the case of calculating Desirable Body Weight of Korean youth, correlation coefficient of r = + 0.38(p < 0.001)between body height and body weight was found the male group and r = + 0.37(p < 0.001) in the female group, from which respective linear regression equation of body weight and height was established for male and female as follows; Male : Y(Body Weight, ㎏) = 0.66 x (Body Height, ㎝) - 48.93 Female :Y(Body Weight, ㎏) = 0.56 x (Body Height, ㎝) - 36.01 4. Formulae for calculating Normal Adapted Body Weight of Korean Adult. ⅰ)Average body height and body weight by age-groups 30-39 age-group Male : 170.4 ±4.9㎝ and 67.6 ±8.1㎏ Female : 158.5 ±3.9㎝ and 53.6 ±6.0㎏ 40-49 age-group Male : 169.1 ±4.9㎝ and 68.0 ±8.5㎏ Female : 157.3 ±4.7㎝ and 56.8 ±7.7㎏ 50-59 age-group Male : 168.1 ±6.8㎝ and 66.0 ±8.1㎏ Female : 157.2 ±4.7㎝ and 57.3 ±7.1㎏ 60-69 age-group Male : 168.0 ±5.3㎝ and 46.7 ±8.7㎏ Female : 155.2 ±5.0㎝ and 56.2 ±9.1㎏ Over 70 age-group Male : 166.1± 6.5㎝ and 62.8±1.2㎏ Female : 152.8 ±5.3㎝ and 52.8 ±8.5㎏ ⅱ) Correlation Coefficient and Linear Regression Equation by Age-groups 30-39 age-group Male : r=+0.44(p<0.001), Y=0.73X-57.94 Female : r=+0.45(p<0.001), Y=0.68X-55.52 40-39 age-group Male : r=+0.54(p<0.001), Y=0.93X-89.92 Female : r=+0.41(p<0.001), Y=0.67X-50.52 50-59 age-group Male : r=+0.32(p<0.001), Y=0.38X-1.22 Female : r=+0.37(p<0.001), Y=0.55X-29.76 60-69 age-group Male : r=+0.51(p<0.001), Y=0.83X-74.84 Female : r=+0.39(p<0.001), Y=0.70X-53.06 over 70 age-group Male : r=+0.63(p<0.001), Y=1.10X-119.96 Female : r=+0.40(p<0.001), Y=0.63X-44.64 Standard Body Weight and Normal Adapted Body Weight of Korean Adult by Age. Using respective regression equation, standard body weight<Table 10, 11>, normal adapted body weight <Table 12~21>, overweight and underweight of the Korean Adult was established for each age group. 6. Obesity Rate of Korean Adult Obesity rate of Korean adult was 9.4% (Male: 9.9%, Female : 8.4%). 7. The Chronological Change on Mean Values of Body Height and Body Weight in Korean Adult The Comparison with the mean values of body height and weight from 1910s to the present 1994 was been remarkably improved, and listed in <Table 23>.

벼 육묘트레이를 이용한 어린 잎 채소 생육에 미치는 광 조절 영향

최기영 ( K. Y. Choi ),김시홍 ( S. H. Kim ),김재경 ( J. K. Kim ),유형주 ( H. J. Yoo ),김일섭 ( I. S. Kim ) 강원대학교 농업생명과학연구원(구 농업과학연구소) 2016 강원 농업생명환경연구 Vol.28 No.2

벼 육묘 트레이를 활용한 어린잎 채소 재배 가능성을 탐색하고자 재배 기간 중 차광 처리하여 다채, 청경채, 알파인배추, 청치마 상추 등 4종을 17일간 재배하였다. 차광 처리는 무차광 17일(NS), 차광 8일+무차광 9일 (S8NS9), 무차광 8일+차광 9일(NS8S9), 차광 17일(S17) 하였다. 처리에 따른 누적 광량은 NS>S8NS9>NS8S9>S17처리구 순으로 높았으나, 누적 온도는 처리에 따른 차이가 크지 않았다 차광 8일 째 어린잎채소 4종의 생육과 엽록소함량은 감소하였다. 광 조절 17일 째 어린잎채소 4종의 초장, 엽장, 엽폭, 경경, 생체중, 엽록소 함량 등의 생육은 무차광 NS 처리구에서 가장 높았다. 생육 초기 광조절에 따른 생육 후기 초장과 생체중 증가율이 어린잎채소 4종 모두 NS 또는 S8NS9 처리구에서 높았다. 따라서 벼 육묘장 다층 시스템에서의 공간 활용을 고려한 어린잎채소 재배시 차광이 많은 하부 공간은 생육초기에 활용하고, 수확기에는 광 확보가 이루어지는 상부에서 재배가 이루어짐이 적합하리라 본다. This study was conducted to investigate the effect of light control on growth of baby leaf vegetables (tatsoi, pak-choi, chinese cabbage ‘alpine’ and leaf lettuce ‘cheongchima’) in rice seedling tray. Light treatment controlled in non-shading (NS), shading for 8 days+non-shading for 9 days (S8NS9), non-shading for 8 days+shading for 9 days (NS8S9), and shading for 17 days (S17). Integrated solar radiation in NS was highest, and in S8NS9>NS8S9>S17 order. Integrated temperature did not shown difference among the treatments for growing periods. Growth and chlorophyll content of baby leaf vegetables were lowest in shading treatment for 8days. The highest growth characteristics (plant height, leaf length, leaf width, stem diameters, and fresh weight) and chlorophyll content of baby leaf vegetables was shown in NS. Increment rate of plant height and fresh weight of baby leaf vegetables were high in NS or S8NS9 as affected by light control for 8 days in early growth periods. These results suggest that we can utilize in low layer for early period and in high layer for harvest periods for baby vegetable production in rice seedling factory.

Production of soluble truncated spike protein of porcine epidemic diarrhea virus from inclusion bodies of Escherichia coli through refolding

Piao, D.C.,Lee, Y.S.,Bok, J.D.,Cho, C.S.,Hong, Z.S.,Kang, S.K.,Choi, Y.J. Academic Press 2016 Protein expression and purification Vol.126 No.-

The emergence of highly pathogenic variant porcine epidemic diarrhea virus (PEDV) strains, from 2013 to 2014, in North American and Asian countries have greatly threatened global swine industry. Therefore, development of effective vaccines against PEDV variant strains is urgently needed. Recently, it has been reported that the N-terminal domain (NTD) of S1 domain of PEDV spike protein is responsible for binding to the 5-N-acetylneuraminic acid (Neu5Ac), a possible sugar co-receptor. Therefore, the NTD of S1 domain could be an attractive target for the development of subunit vaccines. In this study, the NTD spanning amino acid residues 25-229 (S25-229) of S1 domain of PEDV variant strain was expressed in Escherichia coli BL21 (DE3) in the form of inclusion bodies (IBs). S25-229 IBs were solubilized in 20 mM sodium acetate (pH 4.5) buffer containing 8 M urea and 1 mM dithiothreitol with 95% yield. Solubilized S25-229 IBs were refolded by 10-fold flash dilution and purified by one-step cation exchange chromatography with >95% purity and 20% yield. The CD spectrum of S25-229 showed the characteristic pattern of alpha helical structure. In an indirect ELISA, purified S25-229 showed strong reactivity with mouse anti-PEDV sera. In addition, immunization of mice with 20 μg of purified S25-229 elicited highly potent serum IgG titers. Finally, mouse antisera against S25-229 showed immune reactivity with native PEDV S protein in an immunofluorescence assay. These results suggest that purified S25-229 may have potential to be used as a subunit vaccine against PEDV variant strains.

1987년 한국에서 발생한 렙토스피라병의 혈청역학적 조사

이증훈,박영수,이우곤,김석용,정선식,우준희,박성광,박경희,송영욱,김선영,기정일,최두혁,강성귀,김주완,최강원,김우열,최명식,최인학,장우현,윤성열 대한감염학회 1988 감염 Vol.20 No.3

Human leptospirosis was an unfamiliar disease in Korea until 1984 that outbreak of leptospirosis occurred among farmers and soldiers after field works for harvesting rice. During that time, Lee and Jo confirmed the first Korean cases of leptospirosis by serological test, isolation of causative agent and autopy findings. Afterward several outbreaks occurred also during autumn especially after flood in every years and some characterisitcs of leptospirosis in Korea such as clinical manifestations, serotypes and seroepidemiological features has been revealed by many investigators. Because of the major mode of transmission between rodents and human is by direct contact with leptospiral urine of rodents or contaminated soil by the urine, leptospirosis in Korea has been primarily a disease of person in occupations heavily exposed to contaminated soil or infected urine such as farmer, army and etc. Therefore it seems that leptospirosis is one of the main communicable diseases to be controlled urgently in Korea, for an agricultural people account for almost half of total Korean people. For clarifying the seroepidemiological patterns of human leptospirosis in Korea by sex, month region and main reacting serovars of L. interrogans among acute febrile disease occurred in 1987, 1,773 patient's sers with acute febrile episodes were tested by microagglutination test using 19 representative strains of leptospiral serogroup as antigen. All of those sera were collected from 10 collaborative clinics located in Kyunggi, Kangwon, Chungbuk, Chungnam, Chonbuk, Chonnam province and Seoul. The results wee summerized as follows. 1) Among 1,773 sera of patients with acute febrile episodes, 219 (12.4%) were seropositive to L. interrogans, 487(27.5%) to R. tsutsugamushi, 241(13.6%) to R.typhi and 160(90.0%) to Hantaan virus. 2) Among seropositives to L.interrogans, the male outnumbered the female, 65% and 35%. 3) For age distribution, 26.9% of seropositives to L.interrogans were fifties, 19.6% were forties, 9.1% were sixties, 5.9% were thirties and 4.1% were twenties. 4) Eighty three percent of seropositives had occurred between September and October in 1987 with a peak in September. 5) Main leptospiral serovars reactive to patient's sera were Icterohaemorrhagiae(54.3%), Canicola(31.0%), CH-48(13.2%), Tarassovi(0.9%)and Cynopteri(0.5%). 6) For regional distribution, 65.8% of seropositives to L.interrogans were residents from Chonbuk, 12.3% were Chonnam, 7.3% were Chungnam, 5.5% were Kyunggi and 1.4% were Kangwon.