Design of NI sbRIO Based Working Fluid Level Detector and its Verification

Bingkun Gao,Liyang Jin,Running Gao 보안공학연구지원센터(IJSIP) 2013 International Journal of Signal Processing, Image Vol.6 No.6

Association Between the Pre-mir-218 Polymorphism and Cancer Risk in the Chinese Population: a Meta-Analysis

Gao, Yue,Liu, Yan,Liu, Ge-Li,Ran, Long-Ke,Zeng, Fan,Wu, Jia-Yan,Song, Fang-Zhou Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.6

Background: Several recent studies have explored associations between pre-mir-218 polymorphism (rs11134527) and cancer risk. However, published data are still inconclusive. To obtain a more precise estimation of the relationship in the Chinese population, we carried out a meta-analysis for the first time. Materials and Methods: Through retrieval from the PubMed, Medline, Embase, Web of Science databases, China National Knowledge Infrastructure and the Chinese BioMedical Literature Database, a total of four studies were analyzed with 3,561 cases and 3,628 controls for SNP pre-mir-218 rs11134527. We calculated odds ratios (ORs) and 95% confidence intervals (95%CIs) to explore the strength of associations. Results: The results showed that the rs11134527 polymorphism was associated with decreased cancer risk in GG versus AA and GG versus AA+AG models tested ( GG vs AA: OR=0.82, 95%CI: 0.71-0.94; GG vs AA+AG: OR=0.84, 95%CI: 0.74-0.96), and significantly decreased cervical cancer risk was observed in GG versus AA and GG versus AA+AG models (GG vs AA: OR=0.79, 95%CI: 0.66-0.94; GG vs AA+AG: OR=0.80, 95%CI: 0.68-0.94). However, no significant association between the rs11134527polymorphism and hepatocellular carcinoma risk was observed in all comparison models tested (AG vs AA: OR=0.94, 95%CI: 0.79-1.11; GG vs AA: OR=0.88, 95%CI: 0.70-1.10; GG+AG vs AA: OR=0.92, 95%CI: 0.79-1.08; GG vs AA+AG: OR=0.91, 95%CI: 0.75-1.11). Conclusion: The findings suggest that pre-miR-218 rs11134527 polymorphism may have some relation to cancer development in Chinese. However, well-designed studies with larger sample size and more detailed data are needed to confirm these conclusions.

Higher order plate theory for buckling analysis of plates based on exact solution

Ran Bi,Jun Gao,Seyedmahmoodreza Allahyari 국제구조공학회 2021 Steel and Composite Structures, An International J Vol.40 No.3

In this paper, buckling analyses of composite plate reinforced by Graphen platelate (GPL) is studied. The Halphin-Tsai model is used for obtaining the effective material properties of nano composite plate. The nano composite plate is modeled by Third order shear deformation theory (TSDT). The elastic medium is simulated by Winkler model. Employing nonlinear strains-displacements, stress-strain, the energy equations of plate are obtained and using Hamilton's principal, the governing equations are derived. The governing equations are solved based on Navier method. The effect of GPL volume percent, geometrical parameters of plate and elastic foundation on the buckling load are investigated. Results showed that with increasing GPLs volume percent, the buckling load increases.

The Wedding Dress in Ancient China

Gao Han-Yu,Kim Young-Ran 한복문화학회 2002 한복문화학회 국제학술지 Vol.1 No.-

Withanone-Rich Combination of Ashwagandha Withanolides Restricts Metastasis and Angiogenesis through hnRNP-K

Gao, Ran,Shah, Navjot,Lee, Jung-Sun,Katiyar, Shashank P.,Li, Ling,Oh, Eonju,Sundar, Durai,Yun, Chae-Ok,Wadhwa, Renu,Kaul, Sunil C. American Association for Cancer Research 2014 Molecular Cancer Therapeutics Vol.13 No.12

<P>Ashwagandha is an important herb used in the Indian system of traditional home medicine, Ayurveda. Alcoholic extract (i-Extract) from its leaves and its component, withanone, were previously shown to possess anticancer activity. In the present study, we developed a combination of withanone and withaferin A, major withanolides in the i-Extract, that retained the selective cancer cell killing activity and found that it also has significant antimigratory, -invasive, and -angiogenic activities, in both <I>in vitro</I> and <I>in vivo</I> assays. Using bioinformatics and biochemical approaches, we demonstrate that these phytochemicals caused downregulation of migration-promoting proteins hnRNP-K, VEGF, and metalloproteases and hence are candidate natural drugs for metastatic cancer therapy. <I>Mol Cancer Ther; 13(12); 2930–40. ©2014 AACR</I>.</P>

Subcutaneous Streptococcus dysgalactiae GAPDH vaccine in mice induces a proficient innate immune response

Ran An,Yongli Guo,Mingchun Gao,Junwei Wang 대한수의학회 2023 Journal of Veterinary Science Vol.24 No.5

Background: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) on the surface of Streptococcus dysgalactiae, coded with gapC, is a glycolytic enzyme that was reported to be a moonlighting protein and virulence factor. Objective: This study assessed GAPDH as a potential immunization candidate protein to prevent streptococcus infections. Methods: Mice were vaccinated subcutaneously with recombinant GAPDH and challenged with S. dysgalactiae in vivo. They were then evaluated using histological methods. rGAPDH of mouse bone marrow-derived dendritic cells (BMDCs) was evaluated using immunoblotting, reverse transcription quantitative polymerase chain reaction, and enzyme-linked immunosorbent assay methods. Results: Vaccination with rGAPDH improved the survival rates and decreased the bacterial burdens in the mammary glands compared to the control group. The mechanism by which rGAPDH vaccination protects against S. dysgalactiae was investigated. In vitro experiments showed that rGAPDH boosted the generation of interleukin-10 and tumor necrosis factor-α. Treatment of BMDCs with TAK-242, a toll-like receptor 4 inhibitor, or C29, a toll-like receptor 2 inhibitor, reduced cytokines substantially, suggesting that rGAPDH may be a potential ligand for both TLR2 and TLR4. Subsequent investigations showed that rGAPDH may activate the phosphorylation of MAPKs and nuclear factor-κB. Conclusions: GAPDH is a promising immunization candidate protein for targeting virulence and enhancing immune-mediated protection. Further investigations are warranted to understand the mechanisms underlying the activation of BMDCs by rGAPDH in a TLR2- and TLR4-dependent manner and the regulation of inflammatory cytokines contributing to mastitis pathogenesis.

Phosphodiesterase 4D contributes to angiotensin II-induced abdominal aortic aneurysm through smooth muscle cell apoptosis

Gao Ran,Guo Wenjun,Fan Tianfei,Pang Junling,Hou Yangfeng,Feng Xiaohang,Li Bolun,Ge Weipeng,Fan Tianhui,Zhang Tiantian,Lu Jiakai,Jing He,Jin Mu,Yan Chen,Wang Jing 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Abdominal aortic aneurysm (AAA) is a permanent expansion of the abdominal aorta that has a high mortality but limited treatment options. Phosphodiesterase (PDE) 4 family members are cAMP-specific hydrolyzing enzymes and have four isoforms (PDE4A-PDE4D). Several pan-PDE4 inhibitors are used clinically. However, the regulation and function of PDE4 in AAA remain largely unknown. Herein, we showed that PDE4D expression is upregulated in human and angiotensin II-induced mouse AAA tissues using RT-PCR, western blotting, and immunohistochemical staining. Furthermore, smooth muscle cell (SMC)-specific Pde4d knockout mice showed significantly reduced vascular destabilization and AAA development in an experimental AAA model. The PDE4 inhibitor rolipram also suppressed vascular pathogenesis and AAA formation in mice. In addition, PDE4D deficiency inhibited caspase 3 cleavage and SMC apoptosis in vivo and in vitro, as shown by bulk RNA-seq, western blotting, flow cytometry and TUNEL staining. Mechanistic studies revealed that PDE4D promotes apoptosis by suppressing the activation of cAMP-activated protein kinase A (PKA) instead of the exchange protein directly activated by cAMP (Epac). Additionally, the phosphorylation of BCL2-antagonist of cell death (Bad) was reversed by PDE4D siRNA in vitro, which indicates that PDE4D regulates SMC apoptosis via the cAMP-PKA-pBad axis. Overall, these findings indicate that PDE4D upregulation in SMCs plays a causative role in AAA development and suggest that pharmacological inhibition of PDE4 may represent a potential therapeutic strategy.

Characteristics and significance of heterogeneity of sea−land transitional facies shale gas reservoir in North Guizhou, China

Ran Wang,Shuxun Sang,Jun Jin,Lingyun Zhao,Wei Gao,Wei Fu,Fulun Shi,Ende Deng 한국지질과학협의회 2019 Geosciences Journal Vol.23 No.1

In order to identify the characteristics of the longitudinal heterogeneity of the sea–land transitional facies shale gas reservoir in the upper Yangtze region of North Guizhou, studies on the lithological combination, rock and mineral composition, geochemical parameters and reservoir microanisotropy characteristics of Longtan Formation in the study area are conducted on the basis of core observation, testing of geochemistry and reservoir physical property and well logging interpretation. The studies show that the lithological assemblages of the Longtan Formation are diverse and form an amina interbedding of “sand-mud-coal” with obvious cyclicity characteristics. There is a large longitudinal difference in rock and mineral composition and the average mass fraction of the clay mineral is 39.83%, which is obviously higher than that of the marine shale in North America and South China; the longitudinal heterogeneity of the organic matter abundance is high, with an average of 2.17% in the upper part, and 4.51% in the lower part; in accordance with the results observed with the scanning electron microscope and results calculated through pore fractal, the microscopic pore heterogeneity of the reservoir is high. The comparison and analysis of connecting wells with different scales in the study area show that the control effect of the depositional environment on longitudinal macroscopic heterogeneity of Longtan Formation is obvious, and the longitudinal microscopic heterogeneity is controlled through diagenesis. Meanwhile, studies with main coal mining seam as the seam section division method conclude that the heterogeneity of Coal Seam Sections 4 to 5 and Coal Seam Sections 13 to 15 is significantly smaller than that in other seam sections, and the Coal Seam Sections 4 to 5 and Coal Seam Sections 13 to 15 can be considered as a priority key seam section during development of shale gas.

Comparison of Physicochemical Qualities and Antioxidant Activities of Four Chinese Famous Vinegars

Yaping Gao,Yunhee Jo,Namhyeok Chung,Song-Yi Gu,Gui-Ran Kim,Yong-Jin Jeong,Joong-Ho Kwon 한국식품영양과학회 2015 한국식품영양과학회 학술대회발표집 Vol.2015 No.8

Wolbachia infection in six species of gall wasps and their parasitoids

Zhao Gao-Zhi,Zhu Teng-Ran,Zeng Yang,Zhu Dao-Hong 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.1

Wolbachia are endosymbiotic bacteria that are widely present in nematodes and arthropods and sometimes have a significant impact on the evolution, ecology, and biology of their hosts. The co-occurrence of Wolbachia within both Cynipid gall wasps and their parasitoids has rarely been studied. In this study, we report the occurrence of six species of gall wasps and 10 species of their parasitoids in central China. Wolbachia detection using the wsp gene showed that Wolbachia infected two species of gall wasps as well as their parasitoids, indicating that horizontal transmission of Wolbachia occurs between gall wasps and their parasitoids. Given that parasitoids will kill their hosts, Wolbachia may be horizontally transferred from gall wasps to their parasitoids. Using multilocus sequence typing (MLST) analysis, five new strains of Wolbachia were identified, all of which belonged to su pergroup A. The strains of Wolbachia that infected gall wasps were not the same as those that infected their parasitoids. This result indicated that Wolbachia may evolve independently in parasitoids after they have been transferred from the host gall wasps.