http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
김진규,Rouben M. Aroutiounian,Anna A. Nebish,김진홍 한국방사선산업학회 2015 방사선산업학회지 Vol.9 No.4
Today the biosafety evaluation, a common problem of vital importance, is basedon internationally proved test-systems, standards and techniques. The paradigm of biosafetyincludes multidisciplinary approach, a combination of physical, chemical and biological tests tomonitor the environmental level of pollutants and needs to be improved by modern approaches. The genetic risk of environmental pollutions has long been studied by many researchers. In thisstudy, used was the known sensitive plant test-system, clones of plant Tradescantia (spiderwort)able to detect gene mutations (frequency of mutational events and formation of micronuclei) incombination with chemical and, in some instances, with radiological measurements. In addition,male gametophyte generation of fruit trees was applied as bioindicators of genotoxicity. Theobtained results did not show any significant increase along with wind direction. As for the malegametophyte assay, the fertility of the investigated fruit-trees near to NPP did not significantlydiffer from that of the control point. The influence of the NPP on the male generative system of theinvestigated taxa of fruit trees for the investigated year was not revealed. The system describedneeds to be expanded by species of interest (human) as there is a difficulty to transfer the revealeddose correlations to humans. The development of this idea includes various levels: population(epidemiological studies), individual, cellular, molecular (DNA), etc.
Effect of N-acetyl-L-cysteine on human chronic myeloid leukemia cells KCL22 treated with mitomycin C
Simonyan, Anna,Hovhannisyan, Galina,Aroutiounian, Rouben,Kim, Jin Kyu The Ecological Society of Korea 2014 Journal of Ecology and Environment Vol.37 No.1
The effectiveness of N-acetyl-L-cysteine (NAC) to protect blood cells against Mitomycin C (MMC) induced genotoxicity was investigated in human chronic myeloid leukemia cells (KCL22) using the alkaline comet assay. The comet assay was selected as sensitive and rapid method for analysis of DNA damage and repair in individual cells. NAC treatment alone did not produce any damage in KCL22 cell. But NAC was found to be effective in reducing genotoxic damage in KCL22 cells exposed to MMC. These results confirm the literature data that, given the safety and ability to reduce DNA damage. NAC may be useful to prevent drug-mediated genotoxicity.
Tigran, Harutyunyan,Anna, Karapetyan,Galina, Hovhannisyan,Rouben, Aroutiounian Korean Society of Environmental Biology 2013 환경생물 : 환경생물학회지 Vol.31 No.3
Mycotoxins such as aflatoxin B1 (AFB1), ochratoxin A (OTA) and zearalenone (ZEA) are widespread contaminants of food and feedstuffs. It is very likely, that humans and animals are always exposed to mixtures of mycotoxins rather than to individual compounds. Therefore, risk assessments should consider mixture toxicity data. In the present study the combination of AFB1, OTA and ZEA was tested for genotoxicity in rat bone marrow and blood leukocytes after 15, 30 and 60 days treatment. The level of DNA damage was determined by the comet assay. The tail intensity and Olive tail moment in leukocytes and bone marrow cells were significantly higher than in controls. At the same time, the level of DNA damage in bone marrow cells was higher than in leukocytes. The data suggests that prolonged exposure to mycotoxins combination through food consumption can induce DNA damage contributing to the harmful effects in vivo.
( Harutyunyan Tigran ),( Karapetyan Anna ),( Hovhannisyan Galina ),( Aroutiounian Rouben ) 한국환경생물학회 2013 환경생물 : 환경생물학회지 Vol.31 No.3
Mycotoxins such as aflatoxin B1 (AFB1), ochratoxin A (OTA) and zearalenone (ZEA) are widespread contaminants of food and feedstuffs. It is very likely, that humans and animals are always exposed to mixtures of mycotoxins rather than to individual compounds. Therefore, risk assessments should consider mixture toxicity data. In the present study the combination of AFB1, OTA and ZEA was tested for genotoxicity in rat bone marrow and blood leukocytes after 15, 30 and 60 days treatment. The level of DNA damage was determined by the comet assay. The tail intensity and Olive tail moment in leukocytes and bone marrow cells were significantly higher than in controls. At the same time, the level of DNA damage in bone marrow cells was higher than in leukocytes. The data suggests that prolonged exposure to mycotoxins combination through food consumption can induce DNA damage contributing to the harmful effects in vivo.
Effect of N-acetyl-L-cysteine on human chronic myeloid leukemia cells KCL22 treated with mitomycin C
Anna Simonyan,김진규,Galina Hovhannisyan,Rouben Aroutiounian 한국생태학회 2014 Journal of Ecology and Environment Vol.37 No.1
The effectiveness of N-acetyl-L-cysteine (NAC) to protect blood cells against Mitomycin C (MMC) induced genotoxicitywas investigated in human chronic myeloid leukemia cells (KCL22) using the alkaline comet assay. The comet assay wasselected as sensitive and rapid method for analysis of DNA damage and repair in individual cells. NAC treatment alonedid not produce any damage in KCL22 cell. But NAC was found to be effective in reducing genotoxic damage in KCL22cells exposed to MMC. These results confirm the literature data that, given the safety and ability to reduce DNA damage. NAC may be useful to prevent drug-mediated genotoxicity.
The Cytotoxic Action of New Ag-Porphyrin as a Potential Chemotherapeutic Agent
Babayan Nelli,Tovmasyan Artak,Gevorkyan Ani,Gasparyan Gennadi,Aroutiounian Rouben 한국환경생물학회 2008 환경생물 : 환경생물학회지 Vol.26 No.2
- Earlier we have described new water-soluble Ag-and Zn-derivatives of tetrachloridemeso-tetra(4-N-oxiethylpyridyl) porphyrin(TOEtPyP) as potential anticancer drugs. In this workthe effect of one of these metal porphyrins, TOEtPyP Ag, on the cell population kinetics was stu-died in vitrousing morphological and biochemical techniques. The results suggested thatTOEtPyP Ag action consisted in the simultaneous suppression of the cell growth and activation ofthe cell death. About 40% of the cells were shown to die via apoptotic pathway. So, the porphyrinstudied may be attributed to inducers of both necrotic and apoptotic processes. The results ob-tained support our previous assertion that TOEtPyP Ag may be considered as a potential che-motherapeutic agent.