Genetic Distance among South Indian Breeds of Zebu Cattle Using Random Amplified DNA Markers

Ramesha, K.P.,Saravanan, T.,Rao, M.K.,Appannavar, M.M.,Obi Reddy, A. Asian Australasian Association of Animal Productio 2002 Animal Bioscience Vol.15 No.3

Random Amplified Polymorphic DNA (RAPD) assay was conducted to identify polymorphic markers in Amrithmahal, Krishna Valley, Hallikar, Deoni, Khillari, Ongole and Malnad Gidda breeds of South Indian cattle using twenty six primers. Of the 93 RAPD markers obtained, 53 were present in all breeds, 22 were individual specific and 18 were polymorphic for different breeds. Dual purpose breeds viz., Krishna Valley and Ongole showed less genetic divergence between them as compared to their genetic divergence from draft breeds viz., Amrithmahal, Hallikar and Khillari. Malnad Gidda was found to be a distinctly different from others studied.

Skills Development Programmes and Activities for LIS Professionals in India: A Road Map For South Asian Countries

라메샤,Ramesha, Ramesha Korea Institute of Science and Technology Informat 2011 Journal of Information Science Theory and Practice Vol.42 No.4

IT의 융합과 함께 시작된 새로운 천년을 맞이하면서 도서관 전문직은 새로운 변화에 적응하기 위해 지속적으로 기술개발과 평생학습을 위한 노력을 해야 하는 상황에 직면하고 있다. 이 논문은 인도의 도서관계에서 이러한 상황에서 실무와 교육을 위한 전문지식과 기술을 지속적으로 새로이 하기 위해 마련하고 있는 최신의 프로그램과 활동들을 분석하였다. 특히 이 논문에서는 인도가 가지고 있는 조직적인 인프라와 문헌정보학의 교육과 연구, 실무에 응용할 수 있는 새로운 분야들에 대해 집중적으로 살펴보았다. 이러한 프로그램과 이를 통해 얻은 잠재적인 능력들은 인도는 물론 서아시아를 포함한 인근국가의 프로그램개발과 인프라구축, 나아가서는 인력개발을 위한 협력에도 도움이 될 것이다. The new millennium with mystifying convergence of IT has opened new challenges to LIS profession. This has resulted to continuous skill development and lifelong learning, for capacity building in consonant with contemporary changes. The LIS profession in India has created several mechanisms to organize and conduct several skills development programmes through which professionals are continuously enabled to update their professional knowledge and skills, in practice and teaching. There is a good organizational infrastructure and identified areas related to the recent developmental trends and as such LIS professionals and educators have facilities for updating their knowledge at veritable levels and intervals. The paper has surveyed programmes conducted by different organizations at different levels and highlights the aspects of the organizational infrastructure and the emergent areas of applications in LIS teaching, research and professional practices in India. The selection of areas and subjects for various skill development programmes suggest the competencies acquired by the LIS professionals from India, and the empowerment of their professional knowledge can be of immense value to its neighbouring countries. The paper highlights the knowledge and competencies of the LIS professionals in India and the broad-based facilities available here which can be a model for those countries which are seeking to utilize the LIS human resources from this part of the sub-continent. The paper in this circumstance surveyed on the institutional infrastructure for skill developmental opportunities available in India which would meet such requirements within and to the countries outside in the South Asian region seeking its collaboration in such manpower development work.

Global Scenario of Open Access Publishing: A Decadal Analysis of Directory of Open Access Journals (DOAJ) 2003-2012

Pandita, Ramesh,Ramesha, B. Korea Institute of Science and Technology Informat 2013 Journal of Information Science Theory and Practice Vol.1 No.3

The present study aims to assess the growth of open access journals at a global level. The concept of Open Access (OA) publishing is being well received among academic circles and as a result we can see more and more scholarly content is being made these days available in open access format. The present study is simply an attempt to assess the trend and growth of open access journals during the last decade, viz. for the period 2003-2012, for which data has been retrieved from the Directory of Open Access Journals (DOAJ), which as of date hosts more than 9700 journals from 120 countries across the world covering major languages of the world. But keeping in view the period of our study the data has been retrieved as per our applicability, which as a result confines our study to 8453 journals only. The directory covers 18 main subject areas having 76 sub-disciplines, each having on average 118.53 journals. During the entire decade the number of countries which entered into OA publishing rose from 49 to 120 with a growth of 144%, and if this growth rate continues to be the same for the next five years, viz. by 2018, the world will turn into 100% open access. At the continental level Europe leads the tally by publishing a maximum of 3140 OA journals contributed by 43 countries across Europe, which again is the highest number from any continent.

Fuzzy Rule-Based Fault Location Technique for Thyristor-Controlled Series-Compensated Transmission Lines

A. Naresh Kumar,M. Ramesha,S. Jagadha,Bharathi Gururaj,M. Suresh Kumar,Kommera Chaitanya 한국지능시스템학회 2021 INTERNATIONAL JOURNAL of FUZZY LOGIC and INTELLIGE Vol.21 No.4

Estimating the distance of a transmission line with a flexible alternating current transmission system including a thyristor-controlled series compensator is a challenging task. The distance estimation technique based on a fuzzy rule-based system (FRS) in a thyristor-controlled series-compensated transmission line with multi-location faults is investigated in this study. The Haar wavelet current coefficients of the relaying bus are utilized as inputs to accomplish the distance estimation task. The FRS is illustrated through the Mamdani system in the LabVIEW software. The efficacy of the FRS is studied considering the effects of variation with respect to fault parameters. The main characteristic FRS is that it does not involve any two-end communication links because it employs relay terminal measurements only.

Global Transcriptome Profiling of Xanthomonas oryzae pv. oryzae under in planta Growth and in vitro Culture Conditions

이소의,Ravi Gupta,Ramesha H. Jayaramaiah,이서현,Yiming Wang,박상렬,김선태 한국식물병리학회 2017 Plant Pathology Journal Vol.33 No.5

Xanthomonas oryzae pv. oryzae (Xoo), the causative agent of bacterial blight, is a major threat to rice productivity. Here, we performed RNA-Seq based transcriptomic analysis of Xoo transcripts isolated under in planta growth (on both susceptible and resistant hosts) and in vitro culture conditions. Our in planta extraction method resulted in successful enrichment of Xoo cells and provided RNA samples of high quality. A total of 4,619 differentially expressed genes were identified between in planta and in vitro growth conditions. The majority of the differentially expressed genes identified under in planta growth conditions were related to the nutrient transport, protease activity, stress tolerance, and pathogenicity. Among them, over 1,300 differentially expressed genes were determined to be secretory, including 184 putative type III effectors that may be involved in Xoo pathogenicity. Expression pattern of some of these identified genes were further validated by semi-quantitative RT-PCR. Taken together, these results provide a transcriptome overview of Xoo under in planta and in vitro growth conditions with a focus on its pathogenic processes, deepening our understanding of the behavior and pathogenicity of Xoo.

Evaluation of Bioactive Compounds in Strawberry Fruits by a Targeted Metabolomic Approach

Min Jung Ko(최영환),Ramesha H Jayaramaiah,Ravi Gupta,So Wun Kim,Jae Uk An,Ziyu Wang,Mae Li,Nam Jun Kang,Kwang-Pyo Hong,Jum-Soon Kang,Sun Tae Kim,Young Whan Choi 한국원예학회 2018 한국원예학회 학술발표요지 Vol.2018 No.5

A Study on Behavioral Traits of Library and Information Science Students in South India

Baskaran, S.,Babu, B. Ramesha,Gopalakrishnan, S. Korea Institute of Science and Technology Informat 2013 Journal of Information Science Theory and Practice Vol.1 No.1

Human behaviour normally depends on the environment of the incident and the time of its occurrence. The behaviour of people depends on many factors and these behaviour traits are an important aspect in the Library and Information Science (LIS) field. Hence in this paper an attempt has been made to examine the behaviour traits of LIS students in South India. Out of 400 questionnaires distributed 367 have responded and the response rate is 91.75%. In this survey three aspects comprising student behaviour have been analysed such as Work Environment, Natural Environment, and Social Environment. In the case of Work Environment the respondents were grouped as Workaholic, Impatience, Achievement oriented, Rash nature, and Punctuality. Further, in respect to Natural environment, the respondents are grouped as Complacent, Patience, Easygoing, and Relaxed. Last, the respondents were grouped in the Social Environment as Balancing nature, Magnanimity, Naturalistic, Assertive nature, Dependency, Lucrative, Lonely nature, and Time Based personality. Finally the authors conclude that LIS students need to possess these qualities and behaviours to work in different environments.

Evaluation of Bioactive Compounds in Strawberry Fruits by a Targeted Metabolomic Approach

Min Jung Ko,Ramesha H Jayaramaiah,Ravi Gupta,So Wun Kim,Jae Uk An,Ziyu Wang,Mae Li,Nam Jun Kang,Kwang-Pyo Hong,Jum-Soon Kang,Sun Tae Kim,Young Whan Choi 한국원예학회 2017 원예과학기술지 Vol.35 No.6

Strawberry (Fragaria ×ananassa Duch.) is referred to as a functional fruit because of its high nutritional value. Here, we used a targeted metabolomics approach to investigate the quantitative variation in strawberry bioactive compound concentration across cultivars and breeding lines and during fruit ripening. We also compared the efficiency of 95% ethyl alcohol (EtOH), 50% EtOH, and water in extracting these compounds. High-performance liquid chromatography (HPLC) and multivariate analysis revealed that 50% EtOH extracted bioactive compounds more efficiently than 95% EtOH and water. Screening of targeted bioactive compounds extracted with 50% EtOH in six cultivars and 28 breeding lines revealed high abundance of anthocyanins, flavonols, flavanols, and cinnamic acid, which constituted up to 6.4 mg·g<SUP>-1</SUP> of fresh weight of fruits. The highest concentration of phenolic compounds was observed in breeding lines ‘SB08’, ‘SB11’, ‘Arang’, ‘SB09’, and ‘SB10’. Furthermore, significant changes in the concentration of these compounds occurred during fruit ripening in four strawberry cultivars. Overall, our results highlight the chemotypic relationship between cultivars, breeding lines, and fruit ripening associated-changes in strawberries, and these findings provide a chemometric method for choosing optimal cultivars/breeding lines.

Luteinizing hormone beta gene polymorphism and its effect on semen quality traits and luteinizing hormone concentrations in Murrah buffalo bulls

Reen, Jagish Kour,Kerekoppa, Ramesha,Deginal, Revanasiddu,Ahirwar, Maneesh Kumar,Kannegundla, Uday,Chandra, Satish,Palat, Divya,Das, Dayal Nitai,Kataktalware, Mukund Amritrao,Jeyakumar, Sakthivel,Islo Asian Australasian Association of Animal Productio 2018 Animal Bioscience Vol.31 No.8

Objective: Present investigation was aimed to study the Single Nucleotide Variants of the luteinizing hormone beta ($LH{\beta}$) gene and to analyze their association with the semen quality (fresh and post-thawed frozen semen) and luteinizing hormone (LH) concentrations in Murrah buffalo bulls. Methods: Polymerase chain reaction-single stranded conformational polymorphism (PCR-SSCP) and Sanger sequencing method is used to study genetic variability in $LH{\beta}$ gene. LH assay was carried out using enzyme-linked immunosorbent assay method. A fixed general linear model was used to analyze association of single nucleotide polymorphism (SNP) of $LH{\beta}$ gene with semen quality in 109 and LH concentrations in 80 Murrah bulls. Results: $LH{\beta}$ gene was found to be polymorphic. Total six SNPs were identified in $LH{\beta}$ gene g C356090A, g C356113T, g A356701G, g G355869A, g G356330C, and g G356606T. Single Stranded Conformational Polymorphism variants of pattern 2 of exon 1+pattern 2 of exon 2+pattern 1 of exon 3 had highly significant (p<0.01) effect on sperm concentration (million/mL), percent mass motility, acrosome integrity and membrane integrity in fresh and frozen semen whereas significant (p<0.05) effect was observed on percent live spermatozoa. SSCP variants of pattern 2 of exon 1+pattern 2 of exon 2+pattern 1 of exon 3 had highly significant (p<0.01) effect on luteinizing hormone concentrations too. Conclusion: The observed association between SSCP variants of $LH{\beta}$ gene with semen quality parameters and LH concentrations indicated the possibilities of using $LH{\beta}$ as a candidate gene for identification of markers for semen quality traits and LH concentrations in Murrah buffaloes.

Genetic Distance Study among Deoni Breed of Cattle Using Random Amplified DNA Markers

Appannavar, M.M.,Govindaiah, M.G.,Ramesha, K.P. Asian Australasian Association of Animal Productio 2003 Animal Bioscience Vol.16 No.3

Random amplified polymorphic DNA (RAPD) analysis was done with 19 oligonucleotide primers to study genetic similarities and divergence among different types of Deoni breed of cattle viz., Balankya, Wannera and Waghya. Six random primers produced low to high numbers of polymorphic bands between pooled DNA of different Deoni types. Of the 48 RAPD markers obtained 33 were common to all Deoni types, 3 were individual specific and 12 were polymorphic for different Deoni types. The mean average percentage difference values among Deoni types showed that Balankya and Wannera had less genetic divergence when compared to Waghya.