Comparative Phylogenetic Relationships and Genetic Structure of the Caterpillar Fungus Ophiocordyceps sinensis and Its Host Insects Inferred from Multiple Gene Sequences

Qing-Mei Quan,Qing-Xia Wang,Xue-Li Zhou,Shan Li,Xiao-Ling Yang,Yun-Guo Zhu,Zhou Cheng 한국미생물학회 2014 The journal of microbiology Vol.52 No.2

Ophiocordyceps sinensis (Ascomycota: Ophiocordycipitaceae)is a native fungal parasite of Hepialidae caterpillars and oneof the most economically important medicinal caterpillarfungi in China. However, little is known about the phylogeneticand evolutionary relationships between O. sinensis andits host insects. In this study, nuclear ITS and β-tubulin sequencesfrom O. sinensis and mitochondrial COI, COII, andCytb sequences from its hosts were analyzed across 33 populationssampled from five regions in China. Phylogenetically,both O. sinensis and its hosts were divided into three geographicallycorrelated clades, and their phylogenies were congruent. Analysis of molecular variance and calculated coefficientsof genetic differentiation revealed significant geneticdivergence among the clades within both O. sinensis (FST=0.878, NST=0.842) and its hosts (FST=0.861, NST=0.816). Estimatedgene flow was very low for O. sinensis (Nm=0.04) andthe host insects (Nm=0.04) among these three clades. Manteltests demonstrated a significant correlation (P<0.01) betweenthe genetic distances for O. sinensis and its hosts, as well as asignificant association (P<0.05) between geographic and geneticdistances in both. The similar phylogenetic relationships,geographic distributions, and genetic structure and differentiationbetween O. sinensis and its hosts imply that they have coevolved.

Terrabacter ginsengisoli sp. nov., isolated from ginseng cultivating soil

Mei-Fang Jin,Xiao-Tian Quan,주베르,Qing-Zhen Liu,Hong Shan Yu,임완택 한국미생물학회 2018 The journal of microbiology Vol.56 No.5

A Gram-positive, strictly aerobic, nonmotile, yellowish, coccus- rod-shaped bacterium (designated Gsoil 653T) isolated from ginseng cultivating soil was characterized using a polyphasic approach to clarify its taxonomic position. The strain Gsoil 653T exhibited optimal growth at pH 7.0 on R2A agar medium at 30°C. Phylogenetic analysis based on 16S rRNA gene sequence similarities, indicated that Gsoil 653T belongs to the genus Terrabacter of the family Humibacillus, and was closely related to Terrabacter tumescens DSM 20308T (98.9%), Terrabacter carboxydivorans PY2T (98.9%), Terrabacter terrigena ON10T (98.8%), Terrabacter terrae PPLBT (98.6%), and Terrabacter lapilli LR-26T (98.6%). The DNA G + C content was 70.5 mol%. The major quinone was MK-8(H4). The primary polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidyl-ethanolamine. The predominant fatty acids were iso-C15:0, iso-C16:0, iso-C14:0, and anteiso-C15:0, as in the case of genus Terrabacter, thereby supporting the categorization of strain Gsoil 653T. However, the DNA-DNA relatedness between Gsoil 653T and closely related strains of Terrabacter species was low at less than 31%. Moreover, strain Gsoil 653T could be both genotypically and phenotypically distinguished from the recognized species of the genus Terrabacter. This isolate, therefore, represents a novel species, for which the name Terrabacter ginsengisoli sp. nov. is proposed with the type strain Gsoil 653T (= KACC 19444T = LMG 30325T).

消?者?新性?移?支付?APP使用意愿的影??究

全冬梅 ( Quan Dong Mei ),张明明 ( Zhang Ming Ming ),庆成林 ( Qing Cheng Lin ) 한국외국어대학교 중국연구소 2019 中國硏究 Vol.81 No.-

Mobile payment Apps such as Alipay, WeChat payment and Union-Pay are emerging in endlessly, and the competition in the mobile payment market is becoming increasingly severe. This paper divides the mobile payment Apps into perceived benefits and perceived sacrifices based on their characteristics. By means of questionnaire survey, 350 questionnaires were collected, among which 309 were valid. In addition, the model was tested by reliability analysis, validity analysis, structural equation model and path analysis. Finally, the data processing results show that:Consumer innovation has a positive effect on the perceived benefits of mobile payment Apps, that is, the higher the consumer innovation is, the higher the perceived usefulness, perceived ease of use and perceived entertainment are. Perceived benefits positively influence the perceived value of mobile payment Apps, and then promote the use intention of consumers. Perceived benefits and perceived value play a mediating role between consumers' innovation and their willingness to use mobile payment Apps.The above research conclusions clearly define the impact and function mechanism of consumer innovation on the intention to use mobile payment Apps. Therefore, the following suggestions are put forward to mobile payment enterprises: Position innovation groups and pay attention to the word-of-mouth effect. Optimize product functions and enhance the perceived usefulness of consumers. Simplify operation steps and promote advertising. Reduce payment risks and increase security guarantee.

Terrabacter ginsengisoli sp. nov., isolated from ginseng cultivating soil

Jin, Mei-Fang,Quan, Xiao-Tian,Siddiqi, Muhammad Zubair,Liu, Qing-Zhen,Yu, Hong-Shan,Im, Wan-Taek MICROBIOLOGICAL SOCIETY OF KOREA 2018 JOURNAL OF MICROBIOLOGY -SEOUL- Vol.56 No.5

A Gram-positive, strictly aerobic, nonmotile, yellowish, coccus-rod-shaped bacterium (designated Gsoil <TEX>$653^T$</TEX>) isolated from ginseng cultivating soil was characterized using a polyphasic approach to clarify its taxonomic position. The strain Gsoil <TEX>$653^T$</TEX> exhibited optimal growth at pH 7.0 on R2A agar medium at <TEX>$30^{\circ}C$</TEX>. Phylogenetic analysis based on 16S rRNA gene sequence similarities, indicated that Gsoil <TEX>$653^T$</TEX> belongs to the genus Terrabacter of the family Humibacillus, and was closely related to Terrabacter tumescens DSM <TEX>$20308^T$</TEX> (98.9%), Terrabacter carboxydivorans <TEX>$PY2^T$</TEX> (98.9%), Terrabacter terrigena <TEX>$ON10^T$</TEX> (98.8%), Terrabacter terrae <TEX>$PPLB^T$</TEX> (98.6%), and Terrabacter lapilli <TEX>$LR-26^T$</TEX> (98.6%). The DNA G + C content was 70.5 mol%. The major quinone was MK-8(<TEX>$H_4$</TEX>). The primary polar lipids were phosphatidylglycerol, diphosphatidylglycerol, phosphatidyl-ethanolamine. The predominant fatty acids were <TEX>$iso-C_{15:0}$</TEX>, <TEX>$iso-C_{16:0}$</TEX>, <TEX>$iso-C_{14:0}$</TEX>, and <TEX>$anteiso-C_{15:0}$</TEX>, as in the case of genus Terrabacter, thereby supporting the categorization of strain Gsoil <TEX>$653^T$</TEX>. However, the DNA-DNA relatedness between Gsoil <TEX>$653^T$</TEX> and closely related strains of Terrabacter species was low at less than 31%. Moreover, strain Gsoil <TEX>$653^T$</TEX> could be both genotypically and phenotypically distinguished from the recognized species of the genus Terrabacter. This isolate, therefore, represents a novel species, for which the name Terrabacter ginsengisoli sp. nov. is proposed with the type strain Gsoil <TEX>$653^T$</TEX> (<TEX>$=KACC\;19444^T=LMG\;30325^T$</TEX>).

Cytotoxic T Lymphocytes Elicited by Dendritic Cell-Targeted Delivery of Human Papillomavirus Type-16 E6/E7 Fusion Gene Exert Lethal Effects on CaSki Cells

Wu, Xiang-Mei,Liu, Xing,Jiao, Qing-Fang,Fu, Shao-Yue,Bu, You-Quan,Song, Fang-Zhou,Yi, Fa-Ping Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.6

Human papillomavirus (HPV) is the primary etiologic agent of cervical cancer. Consideration of safety and non human leukocyte antigen restriction, protein vaccine has become the most likely form of HPV therapeutic vaccine, although none have so far been reported as effective. Since tumor cells consistently express the two proteins E6 and E7, most therapeutic vaccines target one or both of them. In this study, we fabricated DC vaccines by transducing replication-defective recombinant adenoviruses expressing E6/E7 fusion gene of HPV-16, to investigate the lethal effects of specific cytotoxic T lymphocytes (CTL) against CaSki cells in vitro. Mouse immature dendritic cells (DC) were generated from bone marrow, and transfected with pAd-E6/E7 to prepare a DC vaccine and to induce specific CTL. The surface expression of CD40, CD68, MHC II and CD11c was assessed by flow cytometry (FCM), and the lethal effects of CTL against CaSki cells were determined by DAPI, FCM and CCK-8 methods. Immature mouse DC was successfully transfected by pAd-E6/E7 in vitro, and the transfecting efficiency was 40%-50%. A DC vaccine was successfully prepared and was used to induce specific CTL. Experimental results showed that the percentage of apoptosis and killing rate of CaSki cells were significantly increased by coculturing with the specific CTL (p <0.05). These results illustrated that a DC vaccine modified by HPV-16 E6/E7 gene can induce apoptosis of CaSki cells by inducing CTL, which may be used as a new strategy for biological treatment of cervical cancer.

Rapid functional screening of effective siRNAs against Plk1 and its growth inhibitory effects in Laryngeal carcinoma cells

( Huan Lan ),( Jiang Zhu ),( Qing Ai ),( Zheng Mei Yang ),( Ying Ji ),( Su Ling Hong ),( Fang Zhou Song ),( You Quan Bu ) 생화학분자생물학회 (구 한국생화학분자생물학회) 2010 BMB Reports Vol.43 No.12

Plk 1 is overexpressed in many human malignancies including laryngeal carcinoma. However, its therapeutic potential has been never examined in laryngeal carcinoma. In the present study, a simple cellular morphology-based strategy was firstly proposed for rapidly screening the effective siRNAs against Plk1. Furthermore, we investigated the effects of Plk1 depletion via a novel identified effective siRNA against Plk1, Plk1 siRNA-607, on human laryngeal carcinoma Hep-2 cells. The results indicated that Plk1 siRNA-607 transfection resulted in a significant inhibition in Plk1 expression in cells, and subsequently caused a dramatic mitotic cell cycle arrest followed by massive apoptotic cell death, and eventually resulted in a significant decrease in growth and viability of the laryngeal carcinoma cells. Taken together, our present study not only suggests a simple strategy for rapidly screening effective siRNAs against Plk1 but also implicates that Plk1 may serve as a potential therapeutic target in human laryngeal carcinoma. [BMB reports 2010; 43(12): 818-823]

Evaluation of the anti-Toxoplasma gondii Activity of Hederagenin in vitro and in vivo

Run-Hui Zhang,Runhao Jin,Hao Deng,Qing-Kun Shen,Zhe-Shan Quan,Chun-Mei Jin 대한기생충학열대의학회 2021 The Korean Journal of Parasitology Vol.59 No.3

Toxoplasma gondii infection is widespread worldwide, not only posing a serious threat to human food safety and animal husbandry, but also endangering human health. The selectivity index was employed to measure anti-T. gondii activity. Hederagenin (HE) exhibited potent anti-T. gondii activity and low cytotoxicity. For this reason, HE was selected for in vivo experiments. HE showed 64.8%±13.1% inhibition for peritoneal tachyzoites in mice, higher than spiramycin 56.8%±6.0%. Biochemical parameters such as alanine aminotransferase, aspartate aminotransferase, glutathione, and malondialdehyde, illustrated that HE was a good inhibitor of T. gondii in vivo. This compound was also effective in relieving T. gondii-induced liver damage. Collectively, it was demonstrated that HE had potential as an anti-T. gondii agent.