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Siddiqi, Muhammad Hanif,Siddiqi, Muhammad Zubair,Ahn, Sungeun,Kim, Yeon-Ju,Yang, Deok Chun Pharmaceutical Society of Great Britain 2014 Journal of pharmacy and pharmacology Vol.66 No.12
<P>This study aimed to investigate the stimulative and pharmacological effects of ginsenoside Rh1 (hereinafter referred to as: Rh1) on differentiation and mineralization of osteoblast and its possible mechanism of action on the expression of bone morphogenetic protein 2 (BMP-2)/Runt-related gene 2 (Runx2) signalling pathways using mouse preosteoblastic MC3T3-E1 cell line as in-vitro model.</P>
Siddiqi, Muhammad Zubair,Siddiqi, Muhammad Hanif,Kim, Yeon-Ju,Jin, Yan,Huq, Md. Amdadul,Yang, Deok-Chun Mary Ann Liebert 2015 Journal of medicinal food Vol.18 No.5
<P>In this study, red ginseng extract (RGE) was converted into high-content minor ginsenosides by fermenting with Bgp1 enzymes at 37 degrees C for 5 days. Compared to the RGE, the minor ginsenoside contents were increased in fermented red ginseng extract (FRGE). Moreover, the amount of minor ginsenosides such as Rh1 (11%) and Rg2 (16%) was slightly augmented, while the level of Rg3 (33%) was significantly increased after bioconversion. Furthermore, we also examined and compared the effect of RGE and FRGE on the differentiation and mineralization of preosteoblastic MC3T3-E1 cells. Similarly, the level of mRNA expression of intracellular alkaline phosphatase (ALP) activity, type-1 collagen (Col-I) was also increased. Based on the comparison, it is clear that the FRGE has improved effects on bone formation and differentiation of preosteoblastic MC3T3-E1 cells.</P>
Ginseng saponins and the treatment of osteoporosis: mini literature review
Siddiqi, Muhammad Hanif,Siddiqi, Muhammad Zubair,Ahn, Sungeun,Kang, Sera,Kim, Yeon-Ju,Sathishkumar, Natarajan,Yang, Dong-Uk,Yang, Deok-Chun The Korean Society of Ginseng 2013 Journal of Ginseng Research Vol.37 No.3
The ginseng plant (Panax ginseng Meyer) has a large number of active ingredients including steroidal saponins with a dammarane skeleton as well as protopanaxadiol and protopanaxatriol, commonly known as ginsenosides, which have antioxidant, anticancer, antidiabetic, anti-adipocyte, and sexual enhancing effects. Though several discoveries have demonstrated that ginseng saponins (ginsenosides) as the most important therapeutic agent for the treatment of osteoporosis, yet the molecular mechanism of its active metabolites is unknown. In this review, we summarize the evidence supporting the therapeutic properties of ginsenosides both in vivo and in vitro, with an emphasis on the different molecular agents comprising receptor activator of nuclear factor kappa-B ligand, receptor activator of nuclear factor kappa-B, and matrix metallopeptidase-9, as well as the bone morphogenetic protein-2 and Smad signaling pathways.
Arthrobacter ginsengisoli sp. nov., isolated from soil of a ginseng field
Siddiqi, Muhammad Zubair,Kim, Yeon-Ju,Hoang, Van-An,Siddiqi, Muhammad Hanif,Huq, Md. Amdadul,Yang, Deok-Chun Springer-Verlag 2014 Archives of microbiology Vol.196 No.12
<P>A Gram-staining-positive, catalase-positive, oxidase-negative, non-motile, non-flagellate and rod-shaped bacterium, was designated as DCY81(T), and isolated from soil of a ginseng field in Pocheon province, Republic of Korea. The 16S rRNA gene sequence analysis revealed that strain DCY81(T) belonged to the genus Arthrobacter. Major fatty acid was anteiso-C15:0, while major polar lipids were diphosphatidyglycerol, phatidyglycerol, phosphatidylinositol, monogalactosyldiacylglycerol (GL1), and dimannosyldiacylglycerol (GL2). The dominant quinone was MK-9(H2). The peptidoglycan type was A3α with an L-Lys-L-Ala-L-Thr-L-Ala interpeptide bridge. The DNA-DNA hybridization relatedness between strain DCY81(T) and Arthrobacter siccitolerans LMG 27359(T) (98.2?%), Arthrobacter sulfonivorans JCM 13520(T) (97.81?%), Arthrobacter scleromae DSM 17756(T) (97.59?%), Arthrobacter oxydans KCTC 3383(T) (97.3?%) was 39.1??0.2, 62.2??1.6, 36.8??1.1 and 48.3??1.6?%, respectively which show that the genotypic separation of strain DCY81(T) from the closest reference strain of the genus Arthrobacter. The DNA G+C content was 65.2?mol%. The genotypic analysis, physiological, and chemotaxonomic results indicate that strain DCY81(T) represents a novel species of the genus Arthrobacter. Therefore, Arthrobacter ginsengisoli sp. nov., is proposed as the type strain (=KCTC 29225(T)?=?JCM 19357(T)).</P>
Ginseng saponins and the treatment of osteoporosis: mini literature review
Muhammad Hanif Siddiqi,Muhammad Zubair Siddiqi,Sungeun Ahn,Sera Kang,Yeon-Ju Kim,Natarajan Sathishkumar,Dong-Uk Yang,Deok-Chun Yang 고려인삼학회 2013 Journal of Ginseng Research Vol.37 No.3
The ginseng plant (Panax ginseng Meyer) has a large number of active ingredients including steroidal saponins with a dammarane skeleton as well as protopanaxadiol and protopanaxatriol, commonly known as ginsenosides, which have antioxidant, anticancer, antidiabetic, anti-adipocyte, and sexual enhancing effects. Though several discoveries have demonstrated that ginseng saponins (ginsenosides) as the most important therapeutic agent for the treatment of osteoporosis, yet the molecular mechanism of its active metabolites is unknown. In this review, we summarize the evidence supporting the therapeutic properties of ginsenosides both in vivo and in vitro, with an emphasis on the different molecular agents comprising receptor activator of nuclear factor kappa-B ligand, receptor activator of nuclear factor kappa-B, and matrix metallopeptidase-9, as well as the bone morphogenetic protein-2 and Smad signaling pathways.
Siddiqi, Nikhat J.,Alhomida, Abdullah S. Korean Society for Biochemistry and Molecular Biol 2003 Journal of biochemistry and molecular biology Vol.36 No.2
Collagen is a family of proteins which consists of several genetically distinct molecular species and is intimately involved in tissue organization, function, differentiation and development. The purpose of this study was to investigate the concentration of different hydroxyproline (Hyp) fractions viz., total, free, peptide-bound, protein-bound, soluble- and insoluble-collagen hydroxyproline (Hyp) in various bovine tissues. Results showed that liver had the highest concentration of free Hyp followed by kidney, brain, spleen, lungs, muscle and heart. Liver also had the highest concentration of peptide-bound collagen Hyp followed by kidney, heart, spleen, lungs, brain and muscle. The concentration of protein-bound collagen Hyp was highest in the liver, followed by kidney, spleen, lungs, muscle, brain and heart. Total Hyp was highest in the liver, followed by kidney, spleen, brain, heart, muscle and lungs. Liver also had significantly high concentration of collagen as compared to other tissues examined (P<0.001). Spleen had the significantly higher concentration of soluble-collagen Hyp when compared to other tissues (P<0.001). This was followed by heart, muscle, lungs, brain, kidney and liver. Heart had the highest concentration of insoluble-collagen Hyp followed by lungs, kidney, liver, muscle, spleen and brain. The variation among the insoluble-collagen Hyp concentration of heart and muscle, spleen and brain was significant (P<0.001). We speculate that these differences could be due to the variation in turn over of rate of collagen metabolism in this species.
Ciceribacter azotifigens sp. nov., a nitrogen-fixing bacterium isolated from activated sludge
Siddiqi, Muhammad Zubair,Choi, Gyu-Min,Im, Wan-Taek Society for General Microbiology 2018 International journal of systematic and evolutiona Vol.68 No.2
<P>A Gram-reaction-negative, catalase-and oxidase-positive, aerobic, transparent, motile and rod-shaped bacterium that was capable of fixing dinitrogen (designated strain A.slu09(T)), isolated from activated sludge, was characterized by a polyphasic approach to clarify its taxonomic position. Strain A.slu09(T) was observed to grow optimally at 30 degrees C and at pH 7.0 on R2A agar medium. Strain A.slu09(T) showed beta-glucosidase activity, converting the major ginsenoside Rd to ginsenoside F2. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain A.slu09(T) belongs to the genus Ciceribacter of the family Rhizobiaceae and was most closely related to Ciceribacter lividus MSSRFBL1(T) (97.8% similarity). The DNA G+C content was 67.2 mol%. The DNA-DNA hybridization value between strain A.slu09(T) and C. lividus KCTC 32403(T) was 16.9 +/- 1.17 %. The major polar lipids were phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, aminophospholipid and two glycolipids, and one unknown phospholipid as a minor lipid. The predominant quinone was ubiquinone-10 (Q-10). The major fatty acids were C-19 : 0 cyclo omega 8c, C-18 : 1 omega 7c and/or C-18 : 1 omega 6c (summed feature 8) and C-18 : 0, a profile that supported the affiliation of A. slu09(T) to the genus Ciceribacter. Moreover, the physiological and biochemical characteristics and low level of DNA-DNA relatedness allowed the phenotypic and genotypic differentiation of strain A.slu09(T) from the recognized species of the genus Ciceribacter. Therefore, strain A.slu09(T) represents a novel species of the genus Ciceribacter, for which the name Ciceribacter azotifigens sp. nov. is proposed. The type strain is A.slu09(T) (=KACC 19080(T)=LMG 29962(T)).</P>