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Coupling method of magnetic memory and eddy current nondestructive testing for retired crankshafts
Chen Ni,Lin Hua,Xiaokai Wang,Zhou Wang,Xunpeng Qin,Zhou Fang 대한기계학회 2016 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.30 No.7
To verify the validity of the Coupling method of magnetic memory and eddy current (CMMEC) testing for crankshafts, we use this technique to test a 12-cylinder V-design diesel crankshaft. First, the stress distribution in the crankshaft was obtained under 12 working conditions using a Finite element (FE) model that complied with the commercial FE code ABAQUS. Second, Magnetic memory testing (MMT) and Eddy current testing (ECT) were adopted to detect the regions of stress concentration in the crankshaft and the specific location of cracks based on simulation results. Lastly, magnetic particle testing was conducted to detect and display the corresponding crack to verify the CMMEC testing results. The MMT and ECT results can provide basis and guidance for the remanufacture and life evaluation of retired crankshafts.
( Jun-fang Qin ),( Feng-jiao Jin ),( Ning Li ),( Hai-tao Guan ),( Lan Lan ),( Hong Ni ),( Yue Wang ) 생화학분자생물학회 2015 BMB Reports Vol.48 No.5
Stress and its related hormones epinephrine (E) and norepinephrine (NE) play a crucial role in tumor progression. Macrophages in the tumor microenvironment (TME) polarized to M2 is also a vital pathway for tumor deterioration. Here, we explore the underlying role of macrophages in the effect of stress and E promoting breast cancer growth. It was found that the weight and volume of tumor in tumor bearing mice were increased, and dramatically accompanied with the rising E level after chronic stress using social isolation. What is most noteworthy, the number of M2 macrophages inside tumor was up-regulated with it. The effects of E treatment appear to be directly related to the change of M2 phenotype is reproduced in vitro. Moreover, E receptor ADRβ2 involved in E promoting M2 polarization was comprehended simultaneously. Our results imply psychological stress is influential on specific immune system, more essential for the comprehensive treatment against tumors. [BMB Reports 2015; 48(5): 295-300]
Polymorphisms in DNA Repair Genes and Risk of Glioma and Meningioma
Luo, Ke-Qin,Mu, Shi-Qing,Wu, Zhong-Xue,Shi, Yi-Ni,Peng, Ji-Cai Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.1
Polymorphisms in DNA repair genes have been shown to influence DNA repair processes and to modify cancer susceptibility. Here we conducted a case-control study to assess the role of potential SNPs of DNA repair genes on the risk of glioma and meningioma. We included 297 cases and 458 cancer-free controls. Genotyping of XRCC1 Gln399Arg, XRCC1 Arg194Trp, XRCC2 Arg188His, XRCC3 Thr241Met, XRCC4 Ala247Ser, ERCC1 Asn118Asp, ERCC2 Lys751Gln and ERCC5 Asp1558His were performed in a 384-well plate format on the Sequenom MassARRAY platform. XRCC1 Arg194Trp (rs1799782) and ERCC2 Asp312Asn rs1799793 did not follow the HWE in control group, and genotype distributions of XRCC1 Gln399Arg rs25487, XRCC2 Arg188His rs3218536 and ERCC2 Asp312Asn rs1799793 were significantly different between cases and controls (P<0.05). We found XRCC1 399G/G, XRCC1 194 T/T and XRCC3 241T/T were associated with a higher risk when compared with the wild-type genotype. For ERCC5 Asp1558His, we found G/G genotype was associated with elevated susceptibility. In conclusion, our study has shown that XRCC1 Gln399Arg, XRCC1 Arg194Trp, XRCC3 Thr241Met and ERCC5 Asp1558His are associated with risk of gliomas and meningiomas. This finding could be useful in identifying the susceptibility genes for these cancers.
IL-24 Contributes to Neutrophilic Asthma in an IL-17A-Dependent Manner and Is Suppressed by IL-37
Feng Kang-ni,Meng Ping,Zhang Min,Zou Xiao-ling,Li Shuang,Huang Chu-qin,Lai Ke-fang,Li Hong-tao,Zhang Tian-tuo 대한천식알레르기학회 2022 Allergy, Asthma & Immunology Research Vol.14 No.5
Purpose: Neutrophilic asthma is associated with asthma exacerbation, steroid insensitivity, and severe asthma. Interleukin (IL)-24 is overexpressed in asthma and is involved in the pathogenesis of several allergic inflammatory diseases. However, the role and specific mechanism of IL-24 in neutrophilic asthma are unclear. We aimed to elucidate the roles of IL-24 and IL-37 in neutrophilic asthma, the relationships with IL-17A and the mechanisms regulating neutrophilic asthma progression. Methods: Purified human neutrophils were isolated from healthy volunteers, and a cell coculture system was used to evaluate the function of IL-24 in epithelium-derived IL-17A-dependent neutrophil migration. IL-37 or a small interfering RNA (siRNA) targeting IL-24 was delivered intranasally to verify the effect in a murine model of house dust mite (HDM)/lipopolysaccharide (LPS)-induced neutrophilic asthma. Results: IL-24 enhanced IL-17A production in bronchial epithelial cells via the STAT3 and ERK1/2 signaling pathways; this effect was reversed by exogenous IL-37. Anti-IL-17A monoclonal antibodies reduced neutrophil chemotaxis induced by IL-24-treated epithelial cells in vitro. Increased IL-24 and IL-17A expression in the airway epithelium was observed in HDM/LPS-induced neutrophilic asthma. IL-37 administration or IL-24 silencing attenuated neutrophilic asthma, reducing IL-17A levels and decreasing neutrophil airway infiltration, airway hyperresponsiveness, and goblet cell metaplasia. Silencing IL-24 inhibited T-helper 17 (Th17) immune responses, but not Th1 or Th2 immune responses, in the lungs of a neutrophilic asthma model. Conclusions: IL-24 aggravated neutrophilic airway inflammation by increasing epithelium-derived IL-17A production, which could be suppressed by IL-37. Targeting the IL-24/IL-17A signaling axis is a potential strategy, and IL-37 is a potential candidate agent for alleviating neutrophilic airway inflammation in asthma.
Structural, photoluminescent, and dielectric properties of Eu3+-doped Ba0.7Sr0.3TiO3 thin films
Ling Liu,Ni Qin,Dinghua Bao 한국물리학회 2015 Current Applied Physics Vol.15 No.6
Eu3+-doped Ba0.7Sr0.3TiO3 thin films were prepared by a chemical solution deposition method and characterized by X-ray diffraction, field emission scanning electron microscopy, photoluminescence and dielectric measurements. The thin films were well crystallized with a pure perovskite structure. A contraction of the unit cell was observed upon incorporation of Eu3+ ions below 2 mol%, while an expansion occurred as the Eu3+ concentration was further increased above 2 mol%, indicating that Eu3+ ions with different concentrations occupied different lattice sites. Photoluminescence spectra showed two prominent transitions of Eu3+ ions at 594 nm (5D0→7F1) and 618 nm (5D0→7F2) upon excitation at 395 nm (7F0→5L6). There existed two quenching concentrations at 2 mol% and 4 mol% due to different lattice sites of the Eu3+ ions. We also investigated the dielectric properties of the thin films. Our study suggests that Eu3+-doped Ba0.7Sr0.3TiO3 thin films have potential applications in multifunctional optoelectronic devices.
( Mei Rong Bai ),( Jun Ni ),( Su Qin Shen ),( Qiang Huang ),( Jia Xue Wu ),( Yi Chen Le ),( Long Yu ) 생화학분자생물학회(구 한국생화학분자생물학회) 2014 BMB Reports Vol.47 No.11
Aurora-A is a centrosome-localized serine/threonine kinase that is overexpressed in multiple human cancers. We previously reported an intramolecular inhibitory regulation of Aurora-A between its N-terminal regulatory domain (Nt, amino acids [aa] 1-128) and the C-terminal catalytic domain (Cd, aa 129-403). Here, we demonstrate that although both Aurora-A mutants (AurA-K250G and AurA-D294G/Y295G) lacked interactions between the Nt and Cd, they also failed to interact with Ajuba, an essential activator of Aurora-A, leading to loss of kinase activity. Additionally, overexpression of either of the mutants resulted in centrosome amplification and mitotic spindle formation defects. Both mutants were also able to cause G2/M arrest and apoptosis. These results indicate that both K250 and D294/Y295 are critical for direct interaction between Aurora-A and Ajuba and the function of the Aurora-A complex in cell cycle progression. [BMB Reports 2014; 47(11): 631-636]
Identification of pathways and genes associated with cerebral palsy
Qingwen Zhu,Yufei Ni,Jing Wang,Honggang Yin,Qin Zhang,Lingli Zhang,Wenjun Bian,Bo Liang,Lingyin Kong,Liming Xuan,Naru Lu 한국유전학회 2018 Genes & Genomics Vol.40 No.12
Cerebral palsy (CP) is a non-progressive neurological disease, of which susceptibility is linked to genetic and environmental risk factors. More and more studies have shown that CP might be caused by multiple genetic factors, similar to other neurodevelopmental disorders. Due to the high genetic heterogeneity of CP, we focused on investigating related molecular pathways. Ten children with CP were collected for whole-exome sequencing by next-generation sequencing (NGS) technology. Customized processes were used to identify potential pathogenic pathways and variants. Three pathways (axon guidance, transmission across chemical synapses, protein–protein interactions at synapses) with twenty-three genes were identified to be highly correlated with CP. This study showed that the three pathways associated with CP might be the molecular mechanism of pathogenesis. These findings could provide useful clues for developing pathway-based pharmacotherapies. Further studies are required to confirm potential roles for these pathways in the pathogenesis of CP.
Xiaochen Xiong,Xunpeng Qin,Lin Hua,Gang Wan,Shilong Wei,Mao Ni,Zeqi Hu 대한금속·재료학회 2023 METALS AND MATERIALS International Vol.29 No.6
An in-situ follow-up hammering-assisted (FH) wire arc additive manufacturing (WAAM) process is proposed for hydraulicturbine blade repairing. With different hammering intervention temperatures above the austenite recrystallization temperature(Tre-γ), the influence and mechanism of the process on the grain size of prior austenite grains and room-temperaturemartensite, as well as the texture of 0Cr13Ni5Mo deposited layers are systematically studied. The OM, SEM and EBSD areused for characterization. The repairing layer of large-sized blade is dominated with the coarse columnar grains with severalmillimeters in length, and the grain size is rated as grade 0. After the FH process, the prior austenite grains are significantlyrefined to grade 8. As the hammering temperature increases, the recrystallized austenite grains gradually grow and coarsenowing to the higher ambient temperature. FH at 950 ℃, a temperature slightly higher than the Tre-γ can achieve the austenitegrains with excellent grain refinement effect. Meanwhile, thanks to microstructure inheritance, the room-temperature martensiticis also refined from 4.69 to 2.47 μm, and the typical < 100 > fibre texture content in the deposited layer is obviouslyreduced with the texture intensity reduced from 6.68 to 2.95. Furthermore, the yield strength is increased by about 200 MPa. The main strengthening mechanisms are grain refinement strengthening and dislocation strengthening, and the contributionsto the yield strength are 96.1 MPa and 79 MPa respectively. Additionally, the FH process is also expected to simultaneouslyimprove the formability of the blade repaired layer.