Facile Preparation of an Ionic Liquid Composite Mesoporous Polymer as a Solid Phase Extraction Adsorbent for the Separation and Purification of Flavonoids from Chamaecyparis obtusa

Tian, M.,Qiao, J.,Row, K.H. Taylor Francis 2013 Analytical letters Vol.46 No.9

Biodegradable and Porous Poly(lactic-co-glycolic acid) Microbeads for In vitro Evaluation of Negatively Charged Fluorescent Bacteria

Tian Qiao,김수현,이원목,이현정 한국고분자학회 2019 Macromolecular Research Vol.27 No.3

In this study, porous microbeads synthesized by water-oil-water [water/ oil/water (W1/O/W2)] double-emulsion solvent evaporation method exhibited immense potential as biocarriers for the attachment of bacteria. Poly(lactic-co-glycolic acid) (PLGA) was selected as a biodegradable, biocompatible material for fabricating porous microbeads. Ammonium bicarbonate and polyvinyl alcohol concentrations played a significant role in the modification of the microbead size. The microbead surface structure was modified by partial hydrolysis using sodium hydroxide to generate open pores on the surface and a more hydrophilic surface than PLGA itself. To examine the inner morphology of the microbeads, confocal laser scanning microscopy was employed in combination with rhodamine B during the fabrication of porous microbeads. Finally, the attachment of fluorescent bacteria to these porous microbeads was analyzed in terms of affinity between bacteria and porous PLGA microbeads. The affinity of microbeads to negatively charged bacteria was observed as a function of the charges of the PLGA microbead surfaces, indicating that surface-modified PLGA porous microbeads decorated with positively charged chitosan exhibit an enhanced affinity to negatively charged bacteria.

Development of Nested PCR, Multiplex PCR, and Loop-Mediated Isothermal Amplification Assays for Rapid Detection of Cylindrocladium scoparium on Eucalyptus

Tian-Min Qiao,Jing Zhang,Shu-Jiang Li,Shan Han,Tian-Hui Zhu 한국식물병리학회 2016 Plant Pathology Journal Vol.32 No.5

Eucalyptus dieback disease, caused by Cylindrocladiumscoparium, has occurred in last few years in largeEucalyptus planting areas in China and other countries. Rapid, simple, and reliable diagnostic techniquesare desired for the early detection of Eucalyptus diebackof C. scoparium prior to formulation of efficientcontrol plan. For this purpose, three PCR-based methodsof nested PCR, multiplex PCR, loop-mediatedisothermal amplification (LAMP) were developed fordetection of C. scoparium based on factor 1-alpha (tef1)and beta-tubulin gene in this study. All of the threemethods showed highly specific to C. scoparium. Thesensitivities of the nested PCR and LAMP were muchhigher than the multiplex PCR. The sensitivity of multiplexPCR was also higher than regular PCR. C. scopariumcould be detected within 60 min from infectedEucalyptus plants by LAMP, while at least 2 h wasneeded by the rest two methods. Using different Eucalyptustissues as samples for C. scoparium detection,all of the three PCR-based methods showed muchbetter detection results than regular PCR. Base on theresults from this study, we concluded that any of thethree PCR-based methods could be used as diagnostictechnology for the development of efficient strategiesof Eucalyptus dieback disease control. Particularly,LAMP was the most practical method in field applicationbecause of its one-step and rapid reaction, simpleoperation, single-tube utilization, and simple visualizationof amplification products.

Development of Nested PCR, Multiplex PCR, and Loop-Mediated Isothermal Amplification Assays for Rapid Detection of Cylindrocladium scoparium on Eucalyptus

Qiao, Tian-Min,Zhang, Jing,Li, Shu-Jiang,Han, Shan,Zhu, Tian-Hui The Korean Society of Plant Pathology 2016 Plant Pathology Journal Vol.32 No.5

Eucalyptus dieback disease, caused by Cylindrocladium scoparium, has occurred in last few years in large Eucalyptus planting areas in China and other countries. Rapid, simple, and reliable diagnostic techniques are desired for the early detection of Eucalyptus dieback of C. scoparium prior to formulation of efficient control plan. For this purpose, three PCR-based methods of nested PCR, multiplex PCR, loop-mediated isothermal amplification (LAMP) were developed for detection of C. scoparium based on factor 1-alpha (tef1) and beta-tubulin gene in this study. All of the three methods showed highly specific to C. scoparium. The sensitivities of the nested PCR and LAMP were much higher than the multiplex PCR. The sensitivity of multiplex PCR was also higher than regular PCR. C. scoparium could be detected within 60 min from infected Eucalyptus plants by LAMP, while at least 2 h was needed by the rest two methods. Using different Eucalyptus tissues as samples for C. scoparium detection, all of the three PCR-based methods showed much better detection results than regular PCR. Base on the results from this study, we concluded that any of the three PCR-based methods could be used as diagnostic technology for the development of efficient strategies of Eucalyptus dieback disease control. Particularly, LAMP was the most practical method in field application because of its one-step and rapid reaction, simple operation, single-tube utilization, and simple visualization of amplification products.

Collective dynamics in multi-population Hamiltonian systems

Tian Jing,Ying Jiaqi,Qiao Tianli,Qiu Haibo 한국물리학회 2023 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.82 No.12

In this paper, we investigate measure synchronization (MS) in a two-population network of coupled metronomes system. The system consists of N identical metronomes placed on two swing boards, and the two swings are connected by spring of stifness k. Metronomes on each swing represents a single population. We mainly study the collective dynamics of multipopulation Hamiltonian induced by increased couplings, including inter-population and intra-population couplings. It is found that by tuning K for the inter-population coupling intensity and R for the intra-population coupling intensity, the system reaches various MS states at certain critical coupling intensities, including partial MS and complete MS. The occurrence of partial and complete MS are discussed particularly by analyzing the Poincaré sections of the Hamiltonian system. In addition, we fnd correspondence in between the phase-locking dynamics and various MS transitions.

Novel blood-based hypomethylation of SH3BP5 is associated with very early-stage lung adenocarcinoma

Qiao Rong,Zhong Runbo,Liu Chunlan,Di Feifei,Zhang Zheng,Wang Ling,Xu Tian,Wang Yue,Dai Liping,Gu Wanjian,Han Baohui,Yang Rongxi 한국유전학회 2022 Genes & Genomics Vol.44 No.4

Background: Early detection is essential to improve the survival of lung cancer (LC). The quantitative measurement of specific DNA methylation changes in the peripheral blood could provide an efficient strategy for the detection of early cancer. Objective: We applied a candidate approach and assess the association between blood-based SH3BP5 methylation and the risk of lung adenocarcinoma (LUAD) in a case-control cohort. Methods: The methylation level of four CpG sites in the promoter of SH3BP5 gene was quantitatively determined by mass spectrometry in 171 very early-stage LUAD patients (93.6% LUAD at stage I) and 190 age and gender-matched controls. The logistic regression and non-parametric tests were used for the statistical analyses. Results: We observed a significant association between decreased methylation of SH3BP5_CpG_4 in the peripheral blood and increased risk of LUAD (odds ratio (OR) per-10% methylation = 1.51, P = 0.006, FDR = 0.024), and even for the LUAD at stage I (OR per-10% methylation = 1.53, P = 0.006, FDR = 0.024). Moreover, the lower quartile of SH3BP5_CpG_4 methylation was correlated with increased risk for LUAD with a P trend of 0.011. Further investigation disclosed that the hypomethylation of SH3BP5_CpG_4 was mostly associated with LUAD in younger subjects (OR per-10% methylation = 2.02, P = 0.010, age < 55 years old) and probably could be enhanced by advance stage. Conclusion: Our study revealed an association between blood-based SH3BP5 hypomethylation and very early-stage LUAD, which provides a novel support for the blood-based methylation signatures as a potential marker for the evaluation of cancer risk.

Multi-Epitope Fusion Protein Eg mefAg-1 as a Serodiagnostic Candidate for Cystic Echinococcosis in Sheep

Liu Tianli,Wang Xifeng,Tian Zhenzhong,Wang Lixia,Zhang Xingxing,Qiao Jun,Meng Qingling,Gong Shasha,Chen Ying,Cai Xuepeng 대한기생충학ㆍ열대의학회 2019 The Korean Journal of Parasitology Vol.57 No.1

Cystic echinococcosis (CE) in sheep is a hazardous zoonotic parasitic disease that is caused by Echinococcus granulosus (Eg). At present, serological test is an important diagnostic method for Eg infection in domestic animals. Here, a fusion protein Eg mefAg-1 harboring 8 dominant B-cell epitopes of Eg such as antigen B, tetraspanin 1, tetraspanin 6, reticulon and Eg95 was produced in E. coli and evaluated for CE in sheep by indirect ELISA. Eg mefAg-1 showed in ELI- SA a high sensitivity (93.41%) and specificity (99.31%), with a coincidence rate of 97.02%. Overall, it is suggested that the Eg mefAg-1 could be a potential antigen candidate for CE serodiagnosis in sheep.

Microblog User Geolocation by Extracting Local Words Based on Word Clustering and Wrapper Feature Selection

( Hechan Tian ),( Fenlin Liu ),( Xiangyang Luo ),( Fan Zhang ),( Yaqiong Qiao ) 한국인터넷정보학회 2020 KSII Transactions on Internet and Information Syst Vol.14 No.10

Existing methods always rely on statistical features to extract local words for microblog user geolocation. There are many non-local words in extracted words, which makes geolocation accuracy lower. Considering the statistical and semantic features of local words, this paper proposes a microblog user geolocation method by extracting local words based on word clustering and wrapper feature selection. First, ordinary words without positional indications are initially filtered based on statistical features. Second, a word clustering algorithm based on word vectors is proposed. The remaining semantically similar words are clustered together based on the distance of word vectors with semantic meanings. Next, a wrapper feature selection algorithm based on sequential backward subset search is proposed. The cluster subset with the best geolocation effect is selected. Words in selected cluster subset are extracted as local words. Finally, the Naive Bayes classifier is trained based on local words to geolocate the microblog user. The proposed method is validated based on two different types of microblog data - Twitter and Weibo. The results show that the proposed method outperforms existing two typical methods based on statistical features in terms of accuracy, precision, recall, and F1-score.

Effects of Endothelin A Receptor Antagonist BQ123 on Femoral Artery Pressure and Pulmonary Artery Pressure in Broiler Chickens

Wang, Huiyu,Qiao, Jian,Gao, Mingyu,Yang, Ying,Li, Kai,Wang, Jianlin,Tian, Yong,Xu, Tong Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.10

Endothelin-1 (ET-1) is an important factor in regulation of cardiovascular tone in humans and mammals, but the biological function of ET-1 in the avian vascular system has not been determined. The purpose of this study was to characterize the role of endogenous ET-1 in the vascular system of poultry by investigating the effect of endothelin A receptor ($ET_AR$) antagonist BQ123 on the femoral artery pressure (FAP) and the pulmonary artery pressure (PAP) in broiler chickens. First, we found that plasma and lung homogenate ET-1 levels were both increased with age over the seven weeks life cyccle of broiler chickens. Second, 60 min after intravenous injection, BQ123 ($0.4{\mu}g\;kg^{-1}$ and $2.0{\mu}g\;kg^{-1}$, respectively) induced a significant reduction in FAP and PAP (p<0.05). Third, chronic infusion of BQ123 ($2.0{\mu}g\;kg^{-1}$ each time, two times a day) into abdominal cavities led to significant decrease in systolic pressure of the femoral (p<0.05) and pulmonary arteries (p<0.01) in broiler chickens at 7 and 14 days after treatment. Taken together, the $ET_AR$ antagonist BQ123 lead to a significant reduction of FAP and PAP, which suggests that endogenous ET-1 may be involved in the maintenance and regulation of systemic and pulmonary pressure in broiler chickens.

Precise Control of Quantum Confinement in Cesium Lead Halide Perovskite Quantum Dots via Thermodynamic Equilibrium

Dong, Yitong,Qiao, Tian,Kim, Doyun,Parobek, David,Rossi, Daniel,Son, Dong Hee American Chemical Society 2018 NANO LETTERS Vol.18 No.6

<P>Cesium lead halide (CsPbX<SUB>3</SUB>) nanocrystals have emerged as a new family of materials that can outperform the existing semiconductor nanocrystals due to their superb optical and charge-transport properties. However, the lack of a robust method for producing quantum dots with controlled size and high ensemble uniformity has been one of the major obstacles in exploring the useful properties of excitons in zero-dimensional nanostructures of CsPbX<SUB>3</SUB>. Here, we report a new synthesis approach that enables the precise control of the size based on the equilibrium rather than kinetics, producing CsPbX<SUB>3</SUB> quantum dots nearly free of heterogeneous broadening in their exciton luminescence. The high level of size control and ensemble uniformity achieved here will open the door to harnessing the benefits of excitons in CsPbX<SUB>3</SUB> quantum dots for photonic and energy-harvesting applications.</P> [FIG OMISSION]</BR>