Differential Impacts on Bacterial Composition and Abundance in Rhizosphere Compartments between Al-Tolerant and Al-Sensitive Soybean Genotypes in Acidic Soil

Wen Zhong-Ling,Yang Min-Kai,Fazal Aliya,Liao Yong-Hui,Cheng Lin-Run,Hua Xiao-Mei,Hu Dong-Qing,Shi Ji-Sen,Yang Rong-Wu,Lu Gui-Hua,Qi Jin-Liang,Zhi Hong,Qian Qiu-Ping,Yang Yong-Hua 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.8

In this study, two soybean genotypes, i.e., aluminum-tolerant Baxi 10 (BX10) and aluminumsensitive Bendi 2 (BD2), were used as plant materials and acidic red soil was used as growth medium. The soil layers from the inside to the outside of the root are: rhizospheric soil after washing (WRH), rhizospheric soil after brushing (BRH) and rhizospheric soil at two sides (SRH), respectively. The rhizosphere bacterial communities were analyzed by high-throughput sequencing of V4 hypervariable regions of 16S rRNA gene amplicons via Illumina MiSeq. The results of alpha diversity analysis showed that the BRH and SRH of BX10 were significantly lower in community richness than that of BD2, while the WRH exhibited no significant difference between BX10 and BD2. Among the three sampling compartments of the same soybean genotype, WRH had the lowest community richness and diversity while showing the highest coverage. Beta diversity analysis results displayed no significant difference for any compartment between the two genotypes, or among the three different sampling compartments for any same soybean genotype. However, the relative abundance of major bacterial taxa, specifically nitrogen-fixing and/or aluminum-tolerant bacteria, was significantly different in the compartments of the BRH and/or SRH at phylum and genus levels, indicating genotype-dependent variations in rhizosphere bacterial communities. Strikingly, as compared with BRH and SRH, the WRH within the same genotype (BX10 or BD2) always had an enrichment effect on rhizosphere bacteria associated with nitrogen fixation

Knowledge of Human Papillomavirus (HPV) Infection, Cervical Cancer, and HPV Vaccine and its Correlates among Medical Students in Southwest China: a Multi-center Cross-sectional Survey

Wen, Ying,Pan, Xiong-Fei,Zhao, Zhi-Mei,Chen, Feng,Fu, Chun-Jing,Li, Si-Qi,Zhao, Yun,Chang, Hong,Xue, Qing-Ping,Yang, Chun-Xia Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.14

Background: Since cervical cancer can be prevented and controlled through human papillomavirus (HPV) vaccination, it is important to train health care providers and provide them with appropriate knowledge. This study aimed to understand the level of HPV related knowledge among medical students and correlates in Southwest China in order to address any potential gap in their knowledge base. Methods: We conducted a cross-sectional survey among medical students in six selected universities across Southwest China based on a pretested questionnaire regarding HPV infection, cervical cancer, and HPV vaccines. Results: We successfully surveyed 1, 878 medical students, of whom 32.1% were males and 67.9% were females. Their mean age was 20.8 (standard deviation: 1.3) years. 91.8% of them were ethnic Han Chinese, and 43.8% were students in clinical medicine. While 76.5% had heard of HPV only 48.8% knew that the prevention of cervical cancer was possible through HPV vaccination. Only 42.9% of the male and 49.2% of the female students correctly answered over 10 out of 22 questions on HPV related knowledge. Female students appeared to know more about HPV and HPV vaccination (OR: 1.39; 95% CI: 1.11-1.75). In addition, the student knowledge improved with the grade (p<0.001). University courses were the most selected source of knowledge about HPV vaccination (66.4%). 83.6% of males and 91.4% of females were willing to have more HPV related education by experts (p<0.001). Only 10.1% of the students acknowledged that people had asked for their advice regarding HPV vaccination. Conclusions: Our survey indicates that medical students from Southwest China have poor knowledge of HPV and HPV vaccination, but are willing to receive more relevant information. Targeted education should be incorporated into school courses to inform these future health care providers and ensure success of programs for cervical cancer control and prevention.

RNA silencing-mediated resistance is related to biotic/abiotic stresses and cellular RdRp expression in transgenic tobacco plants

( Xiao Liang Wu ),( Wen Cui Hou ),( Mei Mei Wang ),( Xiao Ping Zhu ),( Fang Li ),( Jie Dao Zhang ),( Xin Zheng Li ),( Xing Qi Guo ) 생화학분자생물학회 2008 BMB Reports Vol.41 No.5

응집침전과 펜톤산화, 초음파를 이용한 고농도 난분해성 염색폐수의 처리

강용식 ( Yong Sik Kang ),매계문 ( Qi Wen Mei ),장기 ( Qi Zhang ),한인섭 ( In Sup Han ) 한국물환경학회(구 한국수질보전학회) 2014 한국물환경학회·대한상하수도학회 공동 춘계학술발표회 Vol.2014 No.-

LncRNA A2M-AS1 lessens the injury of cardiomyocytes caused by hypoxia and reoxygenation via regulating IL1R2

Xue‑Lian Song,Fei‑Fei Zhang,Wen‑Jing Wang,Xin‑Ning Li,Yi Dang,Ying‑Xiao Li,Qian Yang,Mei‑Jing Shi,Xiao‑Yong Qi 한국유전학회 2020 Genes & Genomics Vol.42 No.12

Background: Myocardial ischemia and reperfusion injury (MI/RI) is a complex pathophysiological process, which can lead to severe myocardial injury. The long noncoding RNA alpha-2-macroglobulin antisense RNA 1 (A2M-AS1) has been revealed to be abnormally expressed in MI, However, its function in MI and the potential mechanism are still unclear. Objective: To evaluate the functional role of A2M-AS1 in hypoxia/reoxygenation (H/R)-induced neonatal cardiomyocytes and its potential molecular mechanism. Methods: Dataset GSE66360 was obtained from GEO database for analyzing the RNA expression of A2M-AS1 and interleukin 1 receptor type 2 (IL1R2). KEGG pathway enrichment analysis of the genes that co-expressed with A2M-AS1 was performed. Human neonatal cardiomyocytes were subjected to H/R to construct in vitro models. QRT-PCR and Western blot were adopted to test the levels of mRNA and protein. The viability and apoptosis of cardiomyocytes were tested by CCK-8 and flow cytometry assays, respectively. Results: The expression of A2M-AS1 was notably downregulated in H/R-treated cardiomyocytes. Overexpression of A2M-AS1 can notably enhance the cell viability of H/R-damaged cardiomyocytes, whereas knockdown of A2M-AS1 showed the opposite outcomes. Besides, a negative correlation was showed between A2M-AS1 and IL1R2 expression. In H/R-treated cardiomyocytes, overexpression of IL1R2 weakened the promoting proliferation and anti-apoptosis effects caused by overexpressing A2M-AS1, however, IL1R2-knockdown abolished the anti-proliferation and pro-apoptosis effects caused by silencing A2M-AS1. Conclusion: This study demonstrates the potential regulatory role of A2M-AS1/ IL1R2 axis in cardiomyocytes suffered from H/R, and provides insight into the protection of MI/RI.

TGF-β1 Protein Expression in Non-Small Cell Lung Cancers is Correlated with Prognosis

Huang, Ai-Li,Liu, Shu-Guang,Qi, Wen-Juan,Zhao, Yun-Fei,Li, Yu-Mei,Lei, Bin,Sheng, Wen-Jie,Shen, Hong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.19

To investigate the expression intensity and prognostic significance of TGF-${\beta}1$ protein in non-small cell lung cancer (NSCLC), immunohistochemistry was carried out in 194 cases of NSCLC and 24 cases of normal lung tissues by SP methods. The PU (positive unit) value was used to assess the TGF-${\beta}1$ protein expression in systematically selected fields under the microscope with Leica Q500MC image analysis. We found that the TGF-${\beta}1$ PU value was nearly two-fold higher in NSCLC than in normal lung tissues (p=0.000), being associated with TNM stages (p=0.000) and lymph node metastases (p=0.000), but not to patient age, gender, smoking history, tumor differentiation, histological subtype and tumor location (P>0.05). Univariate analysis indicated that patients with high TGF-${\beta}1$ protein expression and lymph node metastases demonstrated a poor prognosis (both p=0.000,). Multivariate analysis showed that TGF-${\beta}1$ protein expression (RR = 2.565, p=0.002) and lymph node metastases (RR=1.874, p=0.030) were also independent prognostic factors. Thus, TGF-${\beta}1$ protein expression may be correlated to oncogenesis and serve as an independent prognostic biomarker for NSCLC.

Identification and characterization of CONSTANS-like genes from Curcuma alismatifolia

Yuan-Yuan Li,Xiao-Huang Chen,Hui-Wen Yu,Qi-Lin Tian,Luan-Mei Lu 한국원예학회 2021 Horticulture, Environment, and Biotechnology Vol.62 No.2

The CONSTANS ( CO ) and CONSTANS - like ( COL ) genes have an important role in the regulation of fl owering in photoperiod-sensitive plants. However, information on the molecular characterization of COL genes in Curcuma alismatifoliais very limited. The objective of this study was to identify and evaluate 8 COL homologs ( CaCOLs ) in the C. alismatifoliatranscriptome. Their structures, phylogenetic relationships and expressing patterns under short-day (SD) and long-day (LD)contexts were investigated. Our results showed that CaCOLs were classifi ed into three groups: CaCOL3 and CaCOL6 in groupI were the most CO - like genes; CaCOL7 and CaCOL8 in group II contains only one B-box; CaCOL1 to 2 and CaCOL4 to 5formed the group III. Phylogenetic analysis of the CaCOLs in Arabidopsis, Rice, Barley, Malaccensis and C. alismatifoliademonstrated that both the B-box and CCT domains were conserved in dicot and monocot plants, but diff erent groups ofCOL genes evolved independently. Diff erent patterns of mRNA accumulation in C. alismatifolia leaves in response to SDand LD treatments were observed. CaCOL1 , CaCOL2 , CaCOL4 and CaCOL7 showed signifi cantly higher expressions inLD treatment than that of SD, indicating that they were potential fl owering inducers, and are candidate genes for research infl owering regulation and circadian controlling in C. alismatifolia plants. Altogether, our study facilitates successful regulationof fl owering in Curcuma species and provides insights for future molecular breeding programs.

Application of Subway Foundation Pit Engineering Risk Assessment:A case study of Qingdao Rock Area, China

Yuan-shun Shen,Peng Wang,Mei-ping Li,Qi-wen Mei 대한토목학회 2019 KSCE JOURNAL OF CIVIL ENGINEERING Vol.23 No.11

The foundation pit construction risk of subway stations is affected by various uncertain factors, which cannot be analyzed quantitatively by current methods. A synthetic evaluation index system for foundation pit construction risk of subway stations is established by analyzing the factors that influence subway station construction. Based on the hierarchy of these factors, a model of three-stage fuzzy synthetic evaluation is proposed, an analytic hierarchy process is used to determine the weight of each stage factor, the fuzzy sets method is used to determine the membership function, and risk ranking is carried out. The proposed method is applied to a subway station construction of the Qingdao Subway Line No. 3 Project. The simulation results show that the method is reasonable and that it should be practical and helpful for other similar projects.

Behavior of circular concrete-filled steel tubular columns under pure torsion

Fa-Xing Ding,QIANG FU,Bing Wen,Qi-shi Zhou,Xue-mei Liu 국제구조공학회 2018 Steel and Composite Structures, An International J Vol.26 No.4

Concrete-filled steel tubular (CFT) columns are commonly used in engineering structures and always subjected to torsion in practice. This paper is thus devoted to investigate the mechanical behavior of circular CFT columns under pure torsion.3D finite element models based on reasonable material constitutive relation were established for analyzing the load-strain (<i>T-–γ</i>) curves of circular CFT columns under pure torsion. The numerical simulation indicated that local bulking of the steel tube in CFT columns was prevented and the shear strength and ductility of the core concrete were significantly improved due to the confinement effect between the steel tube and the core concrete. Based on the results, formulas to predict the torsional ultimate bearing capacity of circular CFT columns were proposed with satisfactory correspondence with experimental results. Besides, formulas of composite shear stiffness and the overall process of the <i>T-–γ</i> relation of circular CFT columns under pure torsion were proposed.

부상분리 기술을 이용한 현장형 조류수거시스템의 발전 과정 및 개발 방향

김종익(Jong Ik Kim),한인섭(Ihn Sup Han),매계문(Qi Wen Mei),조종주(Chong Joo Cho),정순우(Soon Woo Jung) 大韓環境工學會 2017 대한환경공학회지 Vol.39 No.4

현장형 조류수거시스템(이하 OACS)은 녹조 현상이 심각할 때 긴급 대책으로 투입되는 대안 중의 하나로, 대규모 수역에 투입되는 OACS가 갖추어야 할 조건은 신속대응성, 효율성 및 대용량이다. 부상분리 기술을 이용한 OACS의 성능은 부상․포집․수거 공정의 효율에 의해 결정되며, 처리용량 및 효율성에 가장 중요한 인자는 포집과 수거이다. 이들이 자동화되면 전체 작업속도와 일일 처리수량이 증가한다. OACS에 적용된 기술은 진보될수록 장비의 단순화․소형화와 포집과 수거공정 자동화가 발전하여 신속대응성과 효율성이 향상되었으며, 잔류슬러지를 크게 줄여 친환경성과 수질 지속효과를 향상시켰고, 슬러지 수거량도 증가하여 대용량이 실현되는 방향으로 개선되었다. 그 결과 시스템은 조류증식비율을 극복할 수 있는 조류제거량을 달성할 수 있었고, 처리용량 대비 운영비가 감소되어 기술의 경제성을 크게 향상시켰다. On-site Algae Collecting System (OACS) is one of urgent countermeasures to clean the raw water when the algae blooms severely. Rapid reaction capability, high efficiency and large capacity are required when applying OACS to large water areas. The total performance of OACS are always determined by unit process named Flotation, Trapping and Collection. The working efficiency and daily treatment quantity of OACS can be increased when it runs automatically. As the rapid development of OACS technology, in the first place, equipment are miniaturized and simple. And in the second place, automation process from Trapping to Collection are advanced. So, They produce results higher working efficiency, smaller residual sludge on treated water, system’s advanced environmental friendly features and the increased amount of sludge by Collection process to achieve large capacity. Now OACS has overcome the algae multiplication rate to ensured the amount of removal algae. In another aspect, it is high economically feasible because of reducing operation cost against the large capacity.