Identification of MicroRNAs as potential biomarkers for detecting ischemic stroke

Li Kexin,Shen Li,Zheng Pingping,Wang Yanjun,Wang Lijuan,Meng Xiaoli,Lv Yaogai,Xue Zhiqiang,Guo Xin,Zhang Anning,Pan Pan,Bi Chunli,Chen Yang,Feng Tianyu,Li Bo,Jin Lina,Yao Yan 한국유전학회 2022 Genes & Genomics Vol.44 No.1

Background: Increasing epidemic of ischemic stroke (IS) makes it urgent to understand the pathogenesis and regulatory mechanism, previous studies have described microRNAs (miRNAs) is part of the brain's response to ischemia. Objective: The aim of this study was to screen potential biomarkers for the prediction and novel treatment of IS. Methods: Differentially expressed miRNAs were screened from three newly diagnosed IS patients and three controls by RNA sequencing technology. Furthermore, target prediction databases were then used to analysis the target genes of different expressed miRNAs, and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database were used to identify the functions and the main biochemical and signal pathways of differentially expressed target genes. Results: Our results revealed that 27 miRNAs were differentially expressed in IS, among which, hsa-miR-659-5p was the most highly increased and was first found to be associated with IS. In addition, KEGG pathway analyses showed that differentially expressed miRNAs were mainly significantly enriched in lysosome pathway, cytokine-cytokine receptor interaction pathway, spliceosome pathway, base excision repair pathway. Conclusions: miRNAs were involved in IS pathogenesis, and hsa-miR-659-5p, hsa-miR-151a-3p and hsa-miR-29c-5p as the three highest |log2FoldChange| regulation in this study, which may be the biomarkers of IS and need further study.

Decentralized Multiple Control for DC Microgrid with Hybrid Energy Storage

Li Xin,Zheng Taoyin,Guo Panfeng,Huang Jianan,Li Xinyu,Xiong Wei 대한전기학회 2023 Journal of Electrical Engineering & Technology Vol.18 No.2

For a microgrid with hybrid energy storage system , unreasonable power distribution, significant voltage deviation and state-of-charge (SOC) violation are major issues. Conventionally, they are achieved by introducing communication into centralized control or distributed control. This paper proposes a decentralized multiple control to enhance the performance of the system. A low-pass filter based on droop control is applied to battery energy storage system (BESS), and a low-pass difference filter based on proportional-integral (PI) voltage regulation is employed for supercapacitor (SC). The cooperation between them realizes power distribution, in which the average power is compensated by BESS and the high-frequency fluctuation is suppressed by SC. Meanwhile, the voltage deviation is diminished through mode re-division. Considering the SOC imbalance of BESSs and SOC violation of SC, current correction and voltage compensation methods based on local information are proposed to optimize the current sharing of BESSs and ensure the availability of SC, respectively. The advantage of voltage compensation is that, the SC is still available in process of SOC recovery. The simulation model is established on the MATLAB/Simulink environment and the feasibility of proposed strategy is verified by simulation analysis in different scenarios.

Effects of Aqueous Extract of Schizandra Chinensis Fruit on Cadmium-Induced Change of Monoamine Neurotransmitters in Rats

Zheng Lin Zhao,Guang Wen Zhao,Li Li,Meng Quan Li,Li Xin Guan,Xu Dong Yang,Hou Zhong Li,Feng Lin,Jong Rok Lee,Rong Jie Zhao 한국독성학회 2009 Toxicological Research Vol.25 No.1

The effects of aqueous extract of Schizandra Chinensis Fruit (AESC) on cadmium-induced changes of monoamine neurotransmitters in the different brain regions of adult rats were investigated. Male rats were received intraperitoneal (i.p.) administration of CdCl₂ (0.6 ㎎/㎏/d) for 21 days and sacrificed 7 days after the last administration. Concentrations of norepinephrine (NE), dopamine (DA) in striatum and serotonin (5-HT), 5-hydroxyindole acetic acid (5-HIAA) in cortex were measured by HPLC. There were significant decreases of NE, DA, 5-HT and 5-HIAA in Cd intoxicated rats (P < 0.05), while pretreatment with AESC (20 ㎎/㎏/d or 60 mg/kg/d, p.o., 30 min before CdCl₂) greatly inhibited the decrease of monoamine transmitters, respectively (P < 0.05). Also, AESC significantly increased the reduction of glutathione contents and superoxide dismutase activities in cortex induced by CdCl₂. These results suggest that AESC ameliorates Cd-induced depletion of monoamine neurotransmitters in brain through its antioxidant activity.

Active Compounds from Schisandra chinensis Exhibiting Tyrosinase Activity and Melanin Content Inhibition in B16 Melanoma Cells

Zheng-Fei Yan,Jian Guo,Feng-Hua Tian,Xin-Xin Mao,Yu Li,Chang-Tian Li 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.4

Schisandra chinensis has been used as traditional medicine. The structures of isolate active compounds (schisandrin B, deoxyschisandrin, schisandrin C) from S. chinensis were characterized by physical and spectroscopic analyses. Active compounds were tested for their potential to act as anti-melanogenesis or skin-whitening agents by their abilities to inhibit tyrosinase activity in the cell-free mushroom tyrosinase assay and cellular tyrosinase derived from B16 melanoma cells. The tyrosinase inhibitory activity was correlated to the inhibition of melanin productions in α-MSH-stimulated and unstimulated B16 cells. Cellular tyrosinase kinetics were analyzed and showed by Lineweaver- Burk plot. Schisandrin B was minimally cytotoxic (cell viability: 88.99% at 0.75 μM) and the IC50 value for suppression of mushroom tyrosinase activity was estimated as 0.6 μM. Zymography analysis demonstrated schisandrin B’s concentration-dependent effects and the kinetic analysis indicated schisandrin B’s noncompetitive-inhibitory action.

Tribological study of three-dimensionally braided carbon fiber-nylon 6 composites against 316L stainless steel

Li-yun Zheng,Li-xin Zhao,Jing-jun Zhang 한국물리학회 2007 Current Applied Physics Vol.7 No.s1

A new type of material for orthopaedic devices, a three-dimensional braided carbon ber-reinforced nylon 6 (C3D/MCN) composite,was prepared by anionic polymerization. It was characterized by using a block-on-ring wear tester equipped with real-time friction mea-surement capabilities. Scanning electron microscope and an optical microscope were used to study the wear surfaces of the compositesand metallic ring counterpart. The eects of carbon-ber volume fraction, braiding angle, and friction condition on the tribological prop-erties and wear mechanism of C3D/MCN composites were observed. Experiments showed an optimum wear resistance when the carbon-conditions. The wear mechanism under dry sliding conditions is adhesive wear and abrasive wear. The C3D/MCN composites exhibitedmainly abrasive wear under Hank’s-solution-lubricated conditions.

Evaluation of High Butanol/Acetone Ratios in ABE Fermentations with Cassava by Graph Theory and NADH Regeneration Analysis

Zhigang Li,Zhongping Shi,Xin Li,Le Li,Junping Zheng,Zhenggang Wang 한국생물공학회 2013 Biotechnology and Bioprocess Engineering Vol.18 No.4

Higher butanol/acetone ratio is always desirable in ABE fermentation, and this ratio is closely associated with the complicated patterns of metabolic reactions and NADH generation rate. The patterns of acetate/butyrate formation and re-assimilation in multiple closed reaction loops, as well as NADH regeneration in ABE fermentation using different substrates varies. In this study, we evaluated butanol/acetone ratio in ABE fermentations utilizing cassava and corn based media by graph theory and NADH regeneration analysis. The theoretical calculations and experimental data revealed that a lower metabolic strength in butyrate loop and enhanced NADH generation rate were responsible for the achievement of higher butanol/acetone ratio when fermenting cassava based substrate. In traditional fermentations and extractive fermentations with oleyl alcohol/bio-diesel as the extractants when using cassava based substrate, butanol/acetone ratios reached 2.24, 2.84,and 2.19 with the increasing increments of 14.9, 61.4, and 6.8% respectively, while butanol productivities stayed at comparably high levels as compared with those of the fermentations when cultivating on corn based substrate.

Potential Therapeutic Efficacy of Curcumin in Liver Cancer

Dai, Xin-Zheng,Yin, Hai-Tao,Sun, Ling-Fei,Hu, Xiang,Zhou, Chong,Zhou, Yun,Zhang, Wei,Huang, Xin-En,Li, Xiang-Cheng Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.6

Purpose: Liver cancer, one of the most common cancers in China, is reported to feature relatively high morbidity and mortality. Curcumin (Cum) is considered as a drug possessing anti-angiogenic, anti-inflammation and anti-oxidation effect. Previous research has demonstrated antitumor effects in a series of cancers. Materials and Methods: In this study the in vitro cytotoxicity of Cum was measured by MTT assay and pro-apoptotic effects were assessed by DAPI staining and measurement of caspase-3 activity. In vivo anti-hepatoma efficacy of Cum was assessed with HepG2 xenografts. Results: It is found that Cum dose-dependently inhibited cell growth in HepG2 cells with activation of apoptosis. Moreover, Cum delayed the growth of liver cancer in a dose-dependent manner in nude mice. Conclusions: Cum might be a promising phytomedicine in cancer therapy and further efforts are needed to explore this therapeutic strategy.

Anatomical Study of the Accessory Tendon of the Extensor Hallucis Longus Muscle and Its Clinical Application

Yue Li,Jing-Ying Zhang,Xin-Yue Zhao,Li-Ya Pan,De-Hao Jin,He-Xing Xu,Hu-Zhe Cui,Yan-Qun Liu,Xiang-Zheng Qin,Qingyuan Li 대한정형외과학회 2021 Clinics in Orthopedic Surgery Vol.13 No.2

Background: The accessory tendon of the extensor hallucis longus (ATEHL) muscle is a common abnormal structure, and its clinical significance remains debatable. In this study, we provide the incidence of the ATEHL and characterize its morphological types in Asian cadavers and investigate its clinical applications. Methods: The tendons from 50 adult cadaveric feet, fixed in 10% formalin, were analyzed. We measured the length and width of both the ATEHL and the extensor hallucis brevis (EHB). Results: All dissected specimens had an ATEHL. The first metatarsophalangeal joint was surrounded by an accessory tendon that inserted onto the joint capsule and the dorsal base of the proximal phalanx. We classified the ATEHL into 3 types based on their directions. Differences in ATEHL type based on sex were not statistically significant. Conclusions: We found an ATEHL in all cadaveric specimens in this study. We surmise that the ATEHL acts as an antagonist with the EHB when the toe is extending, which might help prevent the occurrence of hallux valgus deformity.

No Association Between Tea Consumption and Risk of Renal Cell Carcinoma: A Meta-analysis of Epidemiological Studies

Hu, Zheng-Hui,Lin, Yi-Wei,Xu, Xin,Chen, Hong,Mao, Ye-Qing,Wu, Jian,Xu, Xiang-Lai,Zhu, Yi,Li, Shi-Qi,Zheng, Xiang-Yi,Xie, Li-Ping Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.3

Objective: To evaluate the association between tea consumption and the risk of renal cell carcinoma. Methods: We searched PubMed, Web of Science and Scopus between 1970 and November 2012. Two evaluators independently reviewed and selected articles based on predetermined selection criteria. Results: Twelve epidemiological studies (ten case-control studies and two cohort studies) were included in the final analysis. In a meta-analysis of all included studies, when compared with the lowest level of tea consumption, the overall relative risk (RR) of renal cell carcinoma for the highest level of tea consumption was 1.03 (95% confidence interval [CI] 0.89-1.21). In subgroup meta-analyses by study design, there was no significant association between tea consumption and renal cell carcinoma risk in ten case-control studies using adjusted data (RR=1.08, 95% CI 0.84-1.40). Furthermore, there was no significant association in two cohort studies using adjusted data (RR=0.95, 95% CI 0.81-1.12). Conclusion: Our findings do not support the conclusion that tea consumption is related to decreased risk of renal cell carcinoma. Further prospective cohort studies are required.

Effects of Aqueous Extract of Schizandra Chinensis Fruit on Cadmium-Induced Change of Monoamine Neurotransmitters in Rats

Zhao, Zheng Lin,Zhao, Guang Wen,Li, Li,Li, Meng Quan,Guan, Li Xin,Yang, Xu Dong,Li, Hou Zhong,Lin, Feng,Lee, Jong-Rok,Zhao, Rong Jie Korean Society of ToxicologyKorea Environmental Mu 2009 Toxicological Research Vol.26 No.1

The effects of aqueous extract of Schizandra Chinensis Fruit (AESC) on cadmium-induced changes of monoamine neurotransmitters in the different brain regions of adult rats were investigated. Male rats were received intraperitoneal (i.p.) administration of CdCl2 (0.6 mg/kg/d) for 21 days and sacrificed 7 days after the last administration. Concentrations of norepinephrine (NE), dopamine (DA) in striatum and serotonin (5-HT), 5-hydroxyindole acetic acid (5-HIAA) in cortex were measured by HPLC. There were significant decreases of NE, DA, 5-HT and 5-HIAA in Cd intoxicated rats (P < 0.05), while pretreatment with AESC (20 mg/kg/d or 60 mg/kg/d, p.o., 30 min before $CdCl_2$) greatly inhibited the decrease of monoamine transmitters, respectively (P < 0.05). Also, AESC significantly increased the reduction of glutathione contents and superoxide dismutase activities in cortex induced by $CdCl_2$. These results suggest that AESC ameliorates Cd-induced depletion of monoamine neurotransmitters in brain through its antioxidant activity.