Clinical Characteristics and Survival Analysis of Breast Cancer Molecular Subtypes with Hepatic Metastases

Ge, Qi-Dong,Lv, Ning,Kong, Ya-Nan,Xie, Xin-Hua,He, Ni,Xie, Xiao-Ming,Wei, Wei-Dong Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.10

Background: The liver is one of the most common metastatic sites of breast cancer, hepatic metastases developing in 6%-25% of patients with breast cancer and being associated with a poor prognosis. The aim of this study was to analyze the survival and clinical characteristics of patients with hepatic metastases from breast cancer of different molecular subtypes and to investigate the prognostic and predictive factors that effect clinical outcome. Methods: We retrospectively studied the charts of 104 patients with breast cancer hepatic metastases diagnosed at Sun Yat-sen University Cancer Center from December 1990 to June 2009. Subtypes were defined as luminal A, luminal B, human epidermal growth factor receptor 2 (HER2) enriched, triple-negative (TN). Prognostic factor correlations with clinical features and treatment approaches were assessed at the diagnosis of hepatic metastases. Results: The median survival time was 16.0 months, and the one-, two- three-, four-, five-year survival rates were 63.5%, 31.7%, 15.6%, 10.8%, and 5.4%, respectively. Median survival periods after hepatic metastases were 19.3 months (luminal A), 13.3 months (luminal B), 18.9 months (HER2-enriched), and 16.1 months (TN, P=0.11). In multivariate analysis, a 2 year-interval from initial diagnosis to hepatic metastasis, treatment with endocrine therapy, and surgery were independent prognostic factors. Endocrine therapy could improve the survival of luminal subtypes (P=0.004) and was a favorable prognostic factor (median survival 23.4 months vs. 13.8 months, respectively, P=0.011). Luminal A group of patients treated with endocrine therapy did significantly better than the Luminal A group of patients treated without endocrine therapy (median survival of 48.9 vs. 13.8 months, P=0.003). Conclusions: Breast cancer subtypes were not associated with survival after hepatic metastases. Endocrine therapy was a significantly favorable treatment for patients with luminal subtype.

Enhancement of Virus-induced Gene Silencing in Tomato by Low Temperature and Low Humidity

Da-Qi Fu,Ben-Zhong Zhu,Hong-Liang Zhu,Hong-Xing Zhang,Yuan-Hong Xie,Wei-Bo Jiang,Xiao-Dan Zhao,Yun-Bo Luo 한국분자세포생물학회 2006 Molecules and cells Vol.21 No.1

Virus-induced gene silencing (VIGS) is an attractive reverse-genetics tool for studying gene function in plants. We showed that silencing of a phytoene desaturase (PDS) gene is maintained throughout TRV-PDSinoculated tomato plants as well as in their flowers and fruit and is enhanced by low temperature (15°C) and low humidity (30%). RT-PCR analysis of the PDS gene revealed a dramatic reduction in the level of PDS mRNA in leaves, flowers and fruits. Silencing of PDS results in the accumulation of phytoene, the desaturase substrate. In addition, the content of chlorophyll a, chlorophyll b and total chlorophyll in the leaves of PDS-silenced plants was reduced by more than 90%. We also silenced the LeEIN2 gene by infecting seedlings, and this suppressed fruit ripenning. We conclude that this VIGS approach should facilitate large-scale functional analysis of genes involved in the development and ripening of tomato.

Effect of Crystal Structure and Hydrogen Bond of Thermoplastic Oxidized Starch on Manufacturing of Starch-Based Biomass Composite

Chuan-Wei Zhang,Fang-Yi Li,Jianfeng Li,Qi Xie,Jie Xu,An-Fu Guo,Cheng-Zhao Wang 한국정밀공학회 2018 International Journal of Precision Engineering and Vol.5 No.3

Starch-based biomass composites were prepared with starch and plant fiber. In order to improve the properties of the compos ites, oxidized starches (OS) were prepared using hydroxyl peroxide as oxidizer, then glycerol was added into OS as plasticizer to produce thermoplastic oxidized starches (TPOS). Crystal structure, hydrogen bonds of TPOS were researched to gain comprehensive views on the mechanism of the property differences between the starch-based composites with native starch, OS and TPOS. The changes of crystal structure were analyzed by X-ray diffraction analysis. Starch intermolecular hydroxyl changes were investigated using the method of infrared spectrum analysis. It was found that the new hydrogen bonds were formed between the plasticizer, oxidizer and starch, and the starch intermolecular bonding force was weakened. The structure of starch crystallization was destructed in TPOS. Differential scanning calorimetry showed that cross reaction with oxidization and plasticization changed the crystal structure, glass transition process was not observed in TPOS. The tensile and compressive strengths of composite based on TPOS were improved greatly and mechanical properties were not weakened obviously with water content increased. All the results indicated that TPOS could improve the properties of starch-based composites.

Effect of Preoperative Tumour Under-Staging on the Long-Term Survival of Patients Undergoing Radical Gastrectomy for Gastric Cancer

Mi Lin,Qi-Yue Chen,Chao-Hui Zheng,Ping Li,Jian-Wei Xie,Jia-Bin Wang,Jian-Xian Lin,Chang-Ming Huang 대한암학회 2021 Cancer Research and Treatment Vol.53 No.4

Purpose This study aimed to evaluate the effect of preoperative tumor staging deviation (PTSD) on the long-term survival of patients undergoing radical gastrectomy for gastric cancer (RGGC). Materials and Methods Clinicopathological data of 2,346 patients who underwent RGGC were retrospectively analyzed. The preoperative tumor-lymph node-metastasis (TNM) under-staging group (uTNM) comprised patients who had earlier preoperative TNM than postoperative TNM, and the no preoperative under-staging group (nTNM) comprised the remaining patients. Results There were 1,031 uTNM (44.0%) and 1,315 nTNM cases (56.0%). Cox prognostic analysis revealed that PTSD independently affected the overall survival (OS) after surgery. The 5-year OS was lower in the uTNM group (41.8%) than in the nTNM group (71.6%). The patients less than 65 years old, with lower American Society of Anaesthesiologists score, 2-5 cm tumor located at the lower stomach, and cT1 or cN0 preoperative staging would more likely undergo D1+ lymph node dissection (LND) in uTNM (p < 0.05). Logistic analyses revealed that tumor size > 2 cm and body mass index ≤ 22.72 kg/m2 were independent risk factors of preoperative TNM tumor under-staging in patients with cT1N0M0 staging (p < 0.05). Conclusion Underestimated tumor staging is not rare, which possibly results in inadequate LND and affects the long-term survival for patients undergoing RGGC. D2 LND should be carefully performed in patients who are predisposed to this underestimation.

Carbon Nanotubes-Supported Well-Dispersed Pd Nanoparticles for the Efficiently Selective Hydrogenation of Benzoic Acid to Synthesize Cyclohexane Carboxylic Acid

Yin Hu,Wei Chen,Qi Wu,Xin Xie,Weiguo Song 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2019 NANO Vol.14 No.1

Pd and carbon nanotube (CNT) composites were prepared by well-dispersed deposition of Pd nanoparticles on commercial CNT, and applied to the selective hydrogenation of benzoic acid (BA) to synthesize cyclohexane carboxylic acid (CCA). The catalysts and the hydrogenation products were analyzed by XRD, TEM, TG, FTIR, UV-Vis absorption, GC and GC-MS, respectively. Hydrogenation process was also optimized through varying the reaction parameters. The results demonstrate that Pd/CNT catalysts possess the highest hydrogenation efficiency, give the full conversion of BA and 100.0% selectivity towards CCA at the optimal hydrogenation conditions, by comparing with some commercial hydrogenation catalysts and Pd/C catalysts with commercial carbonaceous supports. The excellent hydrogenation performance of Pd/CNT is attributed to the stable crystalline CNT support and the high dispersion of Pd nanoparticles. In addition, the protic solvent is also beneficial to lower the activation energy barrier of BA hydrogenation, and further to improve the hydrogenation rate. This work implies that CNT can be potentially chosen as an effective carbonaceous support to prepare Pd/C catalyst with an outstanding performance of BA selective hydrogenation.

Emodin Inhibits Breast Cancer Cell Proliferation through the ERα-MAPK/Akt-Cyclin D1/Bcl-2 Signaling Pathway

Sui, Jia-Qi,Xie, Kun-Peng,Zou, Wei,Xie, Ming-Jie Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.15

Background: The aim of the present study was to investigate the involvement of emodin on the growth of human breast cancer MCF-7 and MDA-MB-231 cells and the estrogen (E2) signal pathway in vitro. Materials and Methods: MTT assays were used to detect the effects of emodin on E2 induced proliferation of MCF-7 and MDA-MB-231 cells. Flow cytometry (FCM) was applied to determine the effect of emodin on E2-induced apoptosis of MCF-7 cells. Western blotting allowed detection of the effects of emodin on the expression of estrogen receptor ${\alpha}$, cyclin D1 and B-cell lymphoma-2 (Bcl-2), mitogen-activated protein kinases (MAPK) and phosphatidylinostiol 3-kinases (PI3K). Luciferase assays were emplyed to assess transcriptional activity of $ER{\alpha}$. Results: Emodin could inhibit E2-induced MCF-7 cell proliferation and anti-apoptosis effects, and arrest the cell cycle in G0/G1 phase, further blocking the effect of E2 on expression and transcriptional activity of $ER{\alpha}$. Moreover, Emodin influenced the ER ${\alpha}$ genomic pathway via downregulation of cyclin D1 and Bcl-2 protein expression, and influenced the non-genomic pathway via decreased PI3K/Akt protein expression. Conclusions: These findings indicate that emodin exerts inhibitory effects on MCF-7 cell proliferation via inhibiting both non-genomic and genomic pathways.

Etoposide-Cisplatin Alternating with Vinorelbine-Cisplatin Versus Etoposide-Cisplatin Alone in Patients with Extensive Disease Combined with Small Cell Lung Cancer

Zhang, Jie,Qi, Hui-Wei,Zheng, Hui,Chen, Mo,Zhu, Jun,Xie, Hui-Kang,Ni, Jian,Xu, Jian-Fang,Zhou, Cai-Cun Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.10

Background: The aim of this study was to evaluate the efficacy of alternating etoposide-cisplatin and vinorelbine-cisplatin (EP-NP) compared with an etoposide-cisplatin (EP) regimen for advanced combined small cell carcinomas. Materials and Methods: Histologically confirmed combined small cell carcinoma patients who met the inclusion criteria were randomly assigned (1:1) into either the EP-NP setting (group A) or the EP setting (group B). The primary endpoint was progression-free survival in patients who received at least one dose of treatment. Results: Eighty-two patients entered into this trial, 42 in group A and 40 in group B. The objective response rates in group A and group B were 42.9% and 32.5%, respectively (p=0.334). Survival analysis showed that median progression-free survival was 6.1 months in group A, which was significantly longer than the 4.1 months in group B (p=0.041). However, as to overall survival, no significant difference was found between the two groups (11.0 vs 10.1 months in groups A and B, respectively, p=0.545). No unexpected side effects were observed in either group. Conclusions: The EP-NP regimen for combined small cell carcinomas prolonged progressio-nfree survival compared with the EP regimen. Further clinical investigations are warranted.

Deep Blue Laser Gain Medium Based on Triphenylamine Substituted Arylfluorene With Improved Photo-Stability

Jingguan Liu,Yan Qian,Qi Wei,Qi Zhang,Linghai Xie,Chulyeon Lee,Hwajeong Kim,Youngkyoo Kim,Ruidong Xia IEEE 2016 IEEE journal of selected topics in quantum electro Vol.22 No.1

<P>We report a novel blue emission triphenylamine substituted arylfluorene (SPhTPA) as organic optical gain medium with low amplified spontaneous emission (ASE) threshold. The optical gain related properties of SPhTPA and its blends with inert polymer, polystyrene (PS), were investigated in detail. This study shows the gain property and photo-stability of SPhTPA have been improved significantly by doping it into PS. The lowest ASE threshold was obtained for the SPhTPA/PS film doped with 20 wt.% SPhTPA. The film of the same doping ratio exhibited high net gain coefficient (27.8 cm(-1)) and low loss coefficient (7.2 cm(-1)). Moreover, the blend film demonstrated a significantly improved photostability. The ASE photo-stability half-life of the blend film was 15 fold higher than the pristine SPhTPA film when pumped in open ambient atmosphere. This paper suggests that this triphenylamine substituted arylfluorene is a highly prospective gain medium and doping it into PS is an efficient approach for developing air-stable SPhTPA-based organic lasers.</P>

Implication of Polymorphisms in DNA Repair Genes in Prognosis of Hepatocellular Carcinoma

Yue, Ai-Min,Xie, Zhen-Bin,Guo, Shu-Ping,Wei, Qi-Dong,Yang, Xiao-Wei Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.1

XRCC1 genetic polymorphisms could be associated with increased risk of various cancer, including hepatocellular carcinoma (HCC), the fifth most common cancer. We here conducted a study to explore the role of selective SNPs of the XRCC1 and XPD genes in the prognosis of HCC. A total of 231 cases were collected, and genotyping of XRCC1 Arg194Trp, XRCC1 Arg399Gln, XPD Lys751Gln and XPD Asp312Asn was performed by duplex polymerase-chain-reaction with the confronting-two-pair primer method. Our findings indicated XRCC1 399Gln/Gln genotype was associated with a significant difference in the median survival time compared with patients carrying Arg/Trp and Arg/Arg genotypes, and individuals with XPD 751 Gln/ Gln genotype had a significantly greater survival time than patients carrying Lys/Lys and Lys/Gln genotypes. The Cox's regression analysis showed individuals carrying XRCC1 399Trp/Trp genotype had 0.55 fold risk of death from HCC than Arg/Arg genotype. Similarly, XPD 751Gln/Gln had a strong decreasein comparison to XPD Lys/Lys carriers with an HR of 0.34. These results suggest that polymorphisms in XRCC1 and XPD may have functional significance in the prognosis of HCC.

Tunable exchange bias in La1.5Sr0.5CoMnO6 double perovskite doped with nonmagnetic Ga ions

Zhang Hongguang,Chen Wei,Xie Liang,Zhao HuiHui,Li Qi 한국물리학회 2022 Current Applied Physics Vol.35 No.-

The crystal structure, electronic structure, and magnetic behaviors of nonmagnetic Ga ions doped double perovskite La1.5Sr0.5CoMnO6 single phase crystals have been investigated. Different from the traditional magnetic dilution effect of nonmagnetic doping, Ga doping in La1.5Sr0.5CoMnO6 enhances the ferromagnetic (FM) exchange interaction of Co3+-O-Mn3+. Moreover, both conventional and spontaneous exchange bias (EB) effects can be tuned by modulating the Ga doping content, which is accompanied by the variation of the Co3+/4+ and Mn3+/4+ and the effective magnetic moment. The EB field and magnetization can be improved by nonmagnetic Ga3+ doping with content lower than 0.2. The evolution of conventional and spontaneous EB effects in La1.5Sr0.5Co1-xGaxMnO6 can be understood in terms of the unidirectional interface anisotropic coupling between FM/anti-FM, and/or FM/spin glass, which is affected by antisite disorder, spin glass, and the uncompensated coupling between Co and Mn.