연속열역학을 이용한 다성분 혼합물의 상평형

김기창,용평순,권영중 江原大學校 産業技術硏究所 1998 産業技術硏究 Vol.18 No.-

Continuous thermodynamics has been applied for modeling of phase equilibria in multicomponent mixtures, to avoid disadvantages of the pseudo-component and dey-component method. In this paper continuous thermodynamic relations formulated by using the Pate-Teja equation of state were adopted for calculations of phase equilibria in natural gas mixtures, crude oil mixtures and mixtures extracted by supercritical CO? fluids. Calculations of phase equilibria were performed by two procedures ; a moment method coupled with the beta distribution function and a quadrature method combined with Gaussian-Legendre polynomials. Calculated results were compared with experimental data. It was showed that continuous thermodynamic frameworks considered in this paper were well-matched to experimental data.

급성 후방십자인대 단독 손상의 보존적 치료 : 전향적 연구 Prospective Study

정영복,김진수,정호중,정평호 대한슬관절학회 2002 대한슬관절학회지 Vol.14 No.2

목적: 후방십자인대 단독 손상 환자에 있어서 보존적 치료를 시행 후 결과를 알아보고자 하였다. 재료 및 방법: 1999년 8월부터 2001년 12월까지 본원에 내원 하여 후방십자인대 단독 손상으로 진단된 11례에 대해서 보존적 치료를 시행하였다. 초기 진단은 이학적 검사와 Telos 기구를 이용한 후방전위 사진(push stress view)으로 하였고 추시 평가는 전향적으로 하였으며 6주, 3개월, 6개월, 1년, 그 후 매년마다 이학적 검사와 International Knee Documentation Committee(IKDC) score와 Orthopadishe Arbeitsguppe Knie(OAK) score를 이용한 임상적 평가를 하는 것을 기준으로 하였으며 6개월 후에는 후방 전위 사진과 단순 전후 및 좌우 방사선을 시행하였다. 치료방법은 6주간 슬관절 완전 신전 상태로 후방 전위 방지 지지대를 부착한 하지 부목 후 6주간 슬관절 후방 전위 방지 보조기를 시행하였다. 이들의 평균 추시 시간은 17.5개월(7~35)이었다. 결과: 11례중 2례에서는 후방전위에 후 외측 회전 불안정성 동반이 발견되어 수술적 치료를 시행하였으며 보존적 치료를 유지하였던 9례에서는 최근 추시 임상적 평가에서 OAK score상 평균 93.2점, IKDC score상 A가 5례, B가 4례이었다. 결론: 동반손상이 없는 후방십자인대 단독 손상에 있어서 보존적 치료가 일차적 치료 방법으로 생각되며 향후 불안정성의 진행과 이차적 퇴행성관절염의 발생 여부 및 상관 관계에 대해서 장기적 추시 관찰 예정이다. Purpose: We assessed prospectively the results of acute, isolated, posterior cruciate ligament injuries treated conservatively. Materials and Methods: Between August 1999 and December 2001, the study population consited of 11 patients. The patients were treated conservatively and were prescribed full extension long leg splint with posterior tibial support for 6 weeks and then posterior tibial support brace for another 6 weeks. Patients completed a sujetive and objective evaluation at 6 weeks, 3 month, 6 month and each year for an average of 17.5 months. Results: Two patients underwent reconstructive surgery because of combined posterolateral rotatory instability. The nine patients were treated conservatively, the mean Orthopadishe Arbeitsguppe Knie score was 93.2 points. International Knee Documentation Committee score in 5 patients was A and 4 patients was B. Conclusion: Our study suggests that patients with acute, isolated posterior cruciate ligament injuries treated conservatively may get good clinical results, but we are planning a long term follow-up evaluation to reveal change in laxity, incidence of arthrosis and their correlation.

기관까지 침범한 후두 열탕화상 1례

김중환,권평중,홍영호 대한이비인후과학회 1981 대한이비인후과학회지 두경부외과학 Vol.24 No.3

안와봉와직염을 병발한 급성상악동염 1례

권평중,민양기,김중환 중앙대부설한국의과학연구소 1979 한국의과학 Vol.11 No.1

Development of a monoclonal antibody against the CD3ε of olive flounder (<i>Paralichthys olivaceus</i>) and its application in evaluating immune response related to CD3ε

Jung, Jae Wook,Lee, Jung Seok,Kim, Young Rim,Im, Se Pyeong,Kim, Si Won,Lazarte, Jassy Mary S.,Kim, Jaesung,Thompson, Kim D.,Suh, Jong Pyo,Jung, Tae Sung Elsevier 2017 Fish & shellfish immunology Vol.65 No.-

<P><B>Abstract</B></P> <P>The T cell receptor (TCR) is the binding site of antigen and is responsible for specifically activating the adaptive immune response. CD3, an essential component of the CD3-TCR complex, is known to be composed of γδ and ε chains in teleost. However, there are few monoclonal antibodies (mAb) available to identify these molecules on T cells, so we aimed to produce a mAb against CD3ε to improve our understanding of T cell immune response in olive flounder (<I>Paralichthys olivaceus)</I>. CD3ε recombinant protein was expressed in yeast, the expression of which was confirmed by SDS-PAGE, MALDI-TOF/TOF MS and Western blot analysis. A CD3ε-specific mAb 4B2 was selected, the specificity of which was examined by confocal microscopy, flow cytometry and RT-PCR, and the mAb was subsequently used to examine the CD3ε lymphocyte population in several different immune organs, with relatively high percentages of these cells seen in trunk-kidney and spleen, while lower percentages were seen in the liver and peripheral blood of olive flounder. During a viral hemorrhagic septicemia virus (VHSV) infection in olive flounder, the number of CD3ε lymphocytes was seen to gradually increase in the liver, spleen and trunk-kidney of infected fish until 7 days post infection (dpi). In peripheral blood, on the other hand, the increase in CD3ε lymphocyte numbers peaked by 3 dpi. These results suggest that CD3ε lymphocytes might be involved in the immune response against VHSV.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Recombinant CD3ε was produced using yeast expression system. </LI> <LI> Monoclonal antibody 4B2 specifically detects the CD3ε lymphocyte in olive flounder. </LI> <LI> CD3ε lymphocytes might be involved in immune response related to VHSV. </LI> </UL> </P>

Characterization of Hagfish (<i>Eptatretus burgeri</i>) Variable Lymphocyte Receptor–Based Antibody and Its Potential Role in the Neutralization of Nervous Necrosis Virus

Jung, Jae Wook,Lee, Jung Seok,Kim, Jaesung,Im, Se Pyeong,Kim, Si Won,Lazarte, Jassy Mary S.,Kim, Young Rim,Chun, Jin Hong,Ha, Min Woo,Kim, Hyeong Su,Thompson, Kim D.,Jung, Tae Sung American Association of Immunologists 2020 Journal of Immunology Vol.204 No.3

<P><B>Key Points</B></P><P><OL><LI>NNV-specific VLRBs were developed using a library screening system.</LI><LI>The binding ability of this Ab was enhanced through modular engineering.</LI><LI>VLRB could be an alternative neutralizing Ab against Betanodavirus infection.</LI></OL></P><P>The variable lymphocyte receptor (VLR) mediates the humoral immune response in jawless vertebrates, including lamprey (<I>Petromyzon marinus</I>) and hagfish (<I>Eptatretus burgeri</I>). Hagfish VLRBs are composed of leucine-rich repeat (LRR) modules, conjugated with a superhydrophobic C-terminal tail, which contributes to low levels of expression in recombinant protein technology. In this study, we screened Ag-specific VLRBs from hagfish immunized with nervous necrosis virus (NNV). The artificially multimerized form of VLRB was constructed using a mammalian expression system. To enhance the level of expression of the Ag-specific VLRB, mutagenesis of the VLRB was achieved in vitro through domain swapping of the LRR C-terminal cap and variable LRR module. The mutant VLRB obtained, with high expression and secretion levels, was able to specifically recognize purified and progeny NNV, and the Ag binding ability of this mutant was increased by at least 250-fold to that of the nonmutant VLRB. Furthermore, preincubation of the Ag-specific VLRB with NNV reduced the infectivity of NNV in E11 cells in vitro, and in vivo experiment. Our results suggest that the newly developed Ag-specific VLRB has the potential to be used as diagnostic and therapeutic reagents for NNV infections in fish.</P>

Neuroimaging Findings in Patients with COVID-19: A Systematic Review and Meta-Analysis

Kim Pyeong Hwa,Kim Minjae,Suh Chong Hyun,Chung Sae Rom,Park Ji Eun,Kim Soo Chin,Choi Young Jun,Lee Jeong Hyun,Kim Ho Sung,Baek Jung Hwan,Choi Choong Gon,Kim Sang Joon 대한영상의학회 2021 Korean Journal of Radiology Vol.22 No.11

Objective: Central nervous system involvement in coronavirus disease 2019 (COVID-19) has been increasingly reported. We performed a systematic review and meta-analysis to evaluate the incidence of radiologically demonstrated neurologic complications and detailed neuroimaging findings associated with COVID-19. Materials and Methods: A systematic literature search of MEDLINE/PubMed and EMBASE databases was performed up to September 17, 2020, and studies evaluating neuroimaging findings of COVID-19 using brain CT or MRI were included. Several cohort-based outcomes, including the proportion of patients with abnormal neuroimaging findings related to COVID-19 were evaluated. The proportion of patients showing specific neuroimaging findings was also assessed. Subgroup analyses were also conducted focusing on critically ill COVID-19 patients and results from studies that used MRI as the only imaging modality. Results: A total of 1394 COVID-19 patients who underwent neuroimaging from 17 studies were included; among them, 3.4% of the patients demonstrated COVID-19-related neuroimaging findings. Olfactory bulb abnormalities were the most commonly observed (23.1%). The predominant cerebral neuroimaging finding was white matter abnormality (17.6%), followed by acute/subacute ischemic infarction (16.0%), and encephalopathy (13.0%). Significantly more critically ill patients had COVID-19-related neuroimaging findings than other patients (9.1% vs. 1.6%; p = 0.029). The type of imaging modality used did not significantly affect the proportion of COVID-19-related neuroimaging findings. Conclusion: Abnormal neuroimaging findings were occasionally observed in COVID-19 patients. Olfactory bulb abnormalities were the most commonly observed finding. Critically ill patients showed abnormal neuroimaging findings more frequently than the other patient groups. White matter abnormalities, ischemic infarctions, and encephalopathies were the common cerebral neuroimaging findings.

Investigation of variable lymphocyte receptors in the alternative adaptive immune response of hagfish against avian influenza virus

Se Pyeong Im,Jung Seok Lee,Si Won Kim,Young Rim Kim,Jae sung Kim,Tae Sung Jung,Jae Wook Jung,Tae Hoon Kim 대한수의학회 2015 대한수의학회 학술대회발표집 Vol.2015 No.-

Generation and characterization of hagfish variable lymphocyte receptor B against glycoprotein of viral hemorrhagic septicemia virus (VHSV)

Lee, Jung Seok,Kim, Jaesung,Im, Se Pyeong,Kim, Si Won,Lazarte, Jassy Mary S.,Jung, Jae Wook,Gong, Tae Won,Kim, Young Rim,Lee, Jeong Ho,Kim, Hyoung Jun,Jung, Tae Sung Elsevier 2018 Molecular immunology Vol.99 No.-

<P><B>Abstract</B></P> <P>Variable lymphocyte receptors B (VLRBs) are non-immunoglobulin components of the humoral immune system in jawless vertebrates including hagfish (<I>Eptatretus burgeri</I>) and lamprey (<I>Petromyzon marinus</I>). Hagfish VLRBs consist of leucine rich repeat (LRR) modules with a superhydrophobic C-terminal tail, the latter of which leads to extremely low expression levels in recombinant protein technology. Here, we present an artificially oligomerized VLRB (arVLRB) that conjugates <I>via</I> the C4bp oligomerization domain derived from human C4b-binding protein (hC4bp) rather than the superhydrophobic tail. The resulting arVLRB had a tightly multimerized form with seven monomeric VLRB arms and showed high expression and secretion levels in a mammalian expression system. To isolate antigen-specific arVLRB, we constructed large VLRB libraries from hagfish immunized with the fish pathogen, viral hemorrhagic septicemia virus (VHSV). The selected arVLRBs were found to recognize various types of antigens, including the recombinant target protein, purified viruses, and progeny viruses, with high antigen binding abilities and specificities. We also performed <I>in vitro</I> affinity maturation of the arVLRBs through LRRCT mutagenesis, and found that this enhanced their antigen-binding properties by at least 125-fold. Our epitope mapping analysis revealed that <SUP>37</SUP>DWDTPL<SUP>42</SUP>, which is located in a region conserved among the glycoproteins of all VHSV isolates, is the recognition epitope of the arVLRBs. Thus, our newly developed arVLRB could prove useful in the development of universal diagnostic tools and/or therapeutic agents for the virus. Together, our novel findings provide valuable insights into hagfish VLRB and its potential use as a novel alternative to conventional antibodies for biotechnological applications.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Superhydrophobic C-termini of hagfish VLRB leads to extremely low expression level. </LI> <LI> C4bp oligomerization domain mediates heptameric VLRB with high binding ability and producttivity. </LI> <LI> <I>In vitro</I> affinity maturation was efficiently carried out by LRRCT mutagenesis. </LI> <LI> Fine epitope mapping revealed <SUP>37</SUP>DWDTPL<SUP>42</SUP> is the recognition epitope of selected arVLRBs. </LI> <LI> The resulting arVLRBs can be used as diagnostic tools or therapeutic agents of VHSV. </LI> </UL> </P>

Radiation-induced esophageal strictures treated with fluoroscopic balloon dilation: clinical outcomes and factors influencing recurrence in 62 patients

Park, Jung-Hoon,Kim, Kun Yung,Song, Ho-Young,Cho, Young Chul,Kim, Pyeong Hwa,Tsauo, Jiaywei,Kim, Min Tae,Jun, Eun Jung,Jung, Hwoon-Yong,Kim, Sung-Bae,Kim, Jong Hoon SAGE Publications 2018 Acta radiologica Vol.59 No.3