MiR-421 Regulates Apoptosis of BGC-823 Gastric Cancer Cells by Targeting Caspase-3

Wu, Jian-Hong,Yao, Yong-Liang,Gu, Tao,Wang, Ze-You,Pu, Xiong-Yong,Sun, Wang-Wei,Zhang, Xian,Jiang, Yi-Biao,Wang, Jian-Jun Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.13

MicroRNAs might act as oncogenes or tumor suppressors in cancer. Recent studies have shown that miR-421 is up-regulated in human gastric cancer. Here, we found that miR-421 was over-expressed in gastric cancer tissues and cell lines. Bioinformatics analysis predicted that the caspase-3 gene was a target of miR-421. Caspase-3 was negatively regulated by miR-421 at the post-transcriptional level. Bax and Bcl-2 were also regulated by miR-421. Moreover, tumor necrosis factor receptor-I and -II, death receptors in the apoptosis pathway, were up-regulated by miR-421. The over-expression of miR-421 promoted gastric cancer cell growth and inhibited apoptosis of the BGC-823 gastric cancer cell line. These observations indicate that miR-421 acts as a tumor promoter by targeting the caspase-3 gene and preventing apoptosis of gastric cancer cells through inhibition of caspase-3 expression. These findings contribute to our understanding of the functions of miR-421 in gastric cancer.

Bevacizumab Regulates Cancer Cell Migration by Activation of STAT3

Wu, Huan-Huan,Zhang, Shuai,Bian, Huan,Li, Xiao-Xu,Wang, Lin,Pu, Yin-Fei,Wang, Yi-Xiang,Guo, Chuan-Bin Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.15

There are numerous clinical cases indicating that long-term use of bevacizumab may increase the invasiveness of tumors. However, to date, little is known about underlying molecular mechanisms. Therefore, the purpose of our study was to investigate effects of bevacizumab in four cancer cells lines (WSU-HN6, CAL27, Tca83, and HeLa). It was found to promote migration and invasion in the WSU-HN6 and Tca83 cases, while exerting inhibitory effects in CAL27 and HeLa cells. The signal transducer and activator of transcription (STAT) 3 inhibitors niclosamide and S3I-201 inhibited the STAT3 signal pathway, which is activated by bevacizumab. These inhibitors also substantially blocked bevacizumab-induced migration of WSU-HN6 and Tca83 cells. Bevacizumab upregulated interleukin (IL)-6 and phosphorylated (p)-STAT3 expression time-dependently. Therefore, we propose that bevacizumab has differential effects on the migration of different cancer cell lines and promotes migration via the IL-6/STAT3 signaling pathway.

RASSF1A Gene Methylation is Associated with Nasopharyngeal Carcinoma Risk in Chinese

Wu, Kun,Xu, Xiao-Ning,Chen, Yu,Pu, Xiao-Lin,Wang, Bo-Yuan,Tang, Xiao-Dan Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.6

In order to explore the association between RASSF1A methylation and nasopharyngeal carcinoma (NPC) risk of Chinese, we carried out a meta-analysis with searches of PubMed, Web of Science, ProQest and Medline databases. Ultimately, 14 articles were identified and analysised using R Software (R version 3.1.2) including meta packages. Overall, we found a significant relationship between RASSF1A methylation and NPC risk (OR 30.7; 95 % CI, 16.71~56.23; z=11.0591; p<0.0001) in a fixed effects model and (OR 32.1; 95% CI, 14.27~72.01; z=8.3984; p<0.0001) in a random effects model pooled. In tissue and NP brushings groups, similar results were found. Hence, our study identified a strong association between RASSF1A methylation and NPC and highlighted a promising potential for RASSF1A methylation in NPC risk prediction of Chinese.

Highly efficient and ultra-rapid adsorption of malachite green by recyclable crab shell biochar

Jia Wu,Jianwei Yang,Pu Feng,Lishan Wen,Guohuan Huang,Chuanhui Xu,Baofeng Lin 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.113 No.-

In this study, waste crab shell was hydrothermally carbonized and then activated to prepare an effectiveand recyclable adsorbent. The adsorption performance of the adsorbent for different dyes was investigatedthrough adsorption experiments, and the results showed that it had the largest adsorption performancefor malachite green. Furthermore, the adsorption performance and mechanism of malachite greenwere investigated by static and dynamic adsorption method. The crab shell biochar could remove6142.5 mg g1 of malachite green within 3 minutes, indicating that the crab shell biochar could ultrarapidand highly efficient adsorb malachite green. In addition, the malachite green adsorbed on the crabshell biochar could be removed by calcination, which achieved the entire regeneration of the crab shellbiochar. The adsorption of malachite green by crab shell biochar adsorbent conformed to the pseudosecond-order kinetic model, the Dubinin-Radushkevich and Langmuir isotherm adsorption model. Physical and chemical characterization indicated that the adsorption mechanism of malachite greenincluded alkaline fading effect, hydrogen bonding and p-p interaction.

Factors Associated with Behaviors Toward End-of-life Care Among Chinese Oncology Nurses: A Cross-Sectional Study

Xiaoyu Wu,Zhihuan Zhou,Yiheng Zhang,Xiaoyan Lin,Meng Zhang,Fulin Pu,Meifen Zhang 한국간호과학회 2021 Asian Nursing Research Vol.15 No.5

Purpose The goal of this study was to describe the current status of oncology nurses' behaviors toward end of life (EOL) care in China and to explore the factors associated with oncology nurses’ behaviors toward EOL care. Methods A cross-sectional design was applied and a convenience sample of 1038 oncology nurses from 22 grade A hospitals were recruited into this study. A general social demographic data questionnaire was administered, and the Chinese version of Nurses’ Behaviors of Caring for Dying Patients Scale was used to assess nurse behavior toward EOL care. The total score ranges from 40 to 200 points. Data were analyzed with SPSS 26.0 software. Results Chinese oncology nurses' average score of holistic EOL care behaviors was 2.97 ± 0.59. Oncology nurses provide physical care most (3.81 ± 0.76), followed by family care (3.02 ± 0.86), and spiritual care (2.37 ± 0.67). Multiple regression analysis showed that a higher frequency of sharing EOL care experience with colleagues, in-service palliative care education, higher level of head nurse support for EOL patient care, more cases of EOL care, higher working position, and nurse's perceived high level of support were positively associated with behavior toward EOL care. These six factors explained 16.2% of the total variance. Conclusions The results may help provide a basis for converting behavior for EOL care among oncology nurses and design interventions to better improve quality of life for EOL patients with cancer in China. Purpose The goal of this study was to describe the current status of oncology nurses' behaviors toward end of life (EOL) care in China and to explore the factors associated with oncology nurses’ behaviors toward EOL care. Methods A cross-sectional design was applied and a convenience sample of 1038 oncology nurses from 22 grade A hospitals were recruited into this study. A general social demographic data questionnaire was administered, and the Chinese version of Nurses’ Behaviors of Caring for Dying Patients Scale was used to assess nurse behavior toward EOL care. The total score ranges from 40 to 200 points. Data were analyzed with SPSS 26.0 software. Results Chinese oncology nurses' average score of holistic EOL care behaviors was 2.97 ± 0.59. Oncology nurses provide physical care most (3.81 ± 0.76), followed by family care (3.02 ± 0.86), and spiritual care (2.37 ± 0.67). Multiple regression analysis showed that a higher frequency of sharing EOL care experience with colleagues, in-service palliative care education, higher level of head nurse support for EOL patient care, more cases of EOL care, higher working position, and nurse's perceived high level of support were positively associated with behavior toward EOL care. These six factors explained 16.2% of the total variance. Conclusions The results may help provide a basis for converting behavior for EOL care among oncology nurses and design interventions to better improve quality of life for EOL patients with cancer in China.

Deep Learning: High-quality Imaging through Multicore Fiber

Liqing Wu,Jun Zhao,Minghai Zhang,Yanzhu Zhang,Xiaoyan Wang,Ziyang Chen,Jixiong Pu 한국광학회 2020 Current Optics and Photonics Vol.4 No.4

Imaging through multicore fiber (MCF) is of great significance in the biomedical domain. Although several techniques have been developed to image an object from a signal passing through MCF, these methods are strongly dependent on the surroundings, such as vibration and the temperature fluctuation of the fiber’s environment. In this paper, we apply a new, strong technique called deep learning to reconstruct the phase image through a MCF in which each core is multimode. To evaluate the network, we employ the binary cross-entropy as the loss function of a convolutional neural network (CNN) with improved U-net structure. The high-quality reconstruction of input objects upon spatial light modulation (SLM) can be realized from the speckle patterns of intensity that contain the information about the objects. Moreover, we study the effect of MCF length on image recovery. It is shown that the shorter the fiber, the better the imaging quality. Based on our findings, MCF may have applications in fields such as endoscopic imaging and optical communication.

Novel tailoring algorithm for abrupt motion artifact removal in photoplethysmogram signals

Limeng Pu,Pedro J. Chacon,Hsiao-Chun Wu,최진우 대한의용생체공학회 2017 Biomedical Engineering Letters (BMEL) Vol.7 No.4

Photoplethysmogram (PPG) signals are widelyused for wearable electronic devices nowadays. The PPGsignal is extremely sensitive to the motion artifacts (MAs)caused by the subject’s movement. The detection andremoval of such MAs remains a difficult problem. Due tothe complicated MA signal waveforms, none of the existingtechniques can lead to satisfactory results. In this paper,a new framework to identify and tailor the abrupt MAs inPPG is proposed, which consists of feature extraction,change-point detection, and MA removal. In order toachieve the optimal performance, a data-dependent framesizedetermination mechanism is employed. Experimentsfor the heart-beat-rate-measurement application have beenconducted to demonstrate the effectiveness of our proposedmethod, by a correct detection rate of MAs at 98% and theaverage heart-beat-rate tracking accuracy above 97%. Onthe other hand, this new framework maintains the originalsignal temporal structure unlike the spectrum-basedapproach, and it can be further applied for the calculationof blood oxygen level (SpO2).

Increasing the Lifetime of Ad Hoc Networks Using Hierarchical Cluster-based Power Management

( Tin-yu Wu ),( Kai-hua Kuo ),( Hua-pu Cheng ),( Jen-wen Ding ),( Wei-tsong Lee ) 한국인터넷정보학회 2011 KSII Transactions on Internet and Information Syst Vol.5 No.1

One inevitable problem in Ad Hoc networks is the limited battery capacity, which explains why portable devices might shut down suddenly when the power of hardware is depleted. Hence, how to decrease the power consumption is an important issue in ad hoc networks. With the development of wireless technology, mobile devices can transmit voices, surf the Internet, download entertaining stuffs, and even support some P2P applications, like sharing real-time streaming. In order to keep the quality stable, the transmission must be continuous and it is thus necessary to select some managers to coordinate all nodes in a P2P community. In addition to assigning jobs to the staffs (children) when needed, these managers (ancestors) are able to reappoint jobs in advance when employees retire. This paper proposed a mechanism called Cluster-based Power Management (CPM) to stabilize the transmissions and increase Time to Live (TTL) of mobile hosts. In our new proposed method, we establish the clusters according to every node`s joining order and capability, and adjust their sleep time dynamically through three different mathematical models. Our simulation results reveal that this proposed scheme not only reduces the power consumption efficiently, but also increases the total TTLs evidently.

Three New 11,20-Epoxy-ent-kauranoids from Isodon rubescens

Xu Liu,Ji Zhou Wu,Rui Zhan,Wei Guang Wang,Xue Du,Yan Li,Peng Zhang,Jian Xin Pu,Han Dong Sun 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.12

Three rare and new 11,20-epoxy-ent-kaurane diterpenoids, named jianshirubesins D-F (1-3), along with one known analogue (4), were isolated from the aerial parts of Isodon rubescens. Their structures were established by analysis of spectroscopic data. Found in the MTT assay to evaluate the cytotoxicity of compounds 1, 2, and 4, only 1 could selectively inhibit certain cell lines from proliferating. In addition, a simple structure-activity relationship discussion might suggest a new bioactive moiety, different from the α,β-unsaturated ketone group.

Effects of artificial diet on the biological characteristics of the ladybeetle Coccinella septempunctata

Liu Chao,Liu Hongling,Wu Xinglong,Xiao Kejun,He Hengguo,Huang Qiong,Pu Deqiang 한국곤충학회 2022 Entomological Research Vol.52 No.4

The ladybeetle Coccinella septempunctata Linnaeus is an important natural enemy of aphids, scale insects, whitefly, and lepidopteran larvae. Mass rearing of this natural enemy is limited due to the lack of effective artificial feed. We compared the biological performance of C. septempunctata, reared on four artificial diets (A, B, C, and D), while the pea aphid Aphis craccivora served as a control treatment (CK). Results showed that the developmental time before emergence ranked from short to long follow as: CK (12.30d)<D(16.66d)<A(17.38d)<C (17.54d)<B (18.57d). The eclosion rate of larvae ranked from high to low follow as: CK (90.00%) > C (87.50%) = D (87.50%) > B(80.00%)> A (57.50%), and new adult weight from high to low follow as: CK (339.50 g*0.0001) > A (205.33 g*0.0001) > D (197.68 g*0.0001) > B (174.89 g*0.0001) > C (169.46 g*0.0001). The ratios of fecundity between the experimental group and the control group were 80.46% (A), 39.24% (B), 45.31% (C), and 53.02% (D). The hatch rates were 59.50% (A), 46.00% (B), 57.65% (C), 54.50% (D), and 53.88% (CK). The mortality of C. septempunctata adults fed on a combined artificial diet was higher than those fed on the control diet. Compared to the control diet, C. septempunctata did not significantly reduce oviposition when fed on artificial diet A. Therefore, diet A can be used in mass-rearing of C. septempunctata.