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Phisit Seesuriyachan 한국식품과학회 2012 Food Science and Biotechnology Vol.21 No.6
In the last two decades, many studies have been reported that a high concentration of NaCl suppresses exopolysaccharide (EPS) production in lactic acid bacteria. In the present study, however, the enhancement of EPS production by Lactobacillus confusus under high salinity stress in submerged fermentation was demonstrated using response surface methodology via a full factorial design. Under the optimized conditions of 3.33% NaCl, 20 g/L sucrose, and 35 h of incubation, the EPS yield was 10.87 g/L with 178% higher than the maximum yield (6.12 g/L of EPS) produced from the modified MRS medium without NaCl. Biomass production was independent of EPS production. A high yield of biomass was obtained in the culture with 0.23% NaCl. This results indicate that high salinity stress by NaCl can enhance EPS production in submerged fermentation in uncontrolled pH cultivations by inducing the production of cell-associated dextransucrase.
Tanitta Royintarat,Phisit Seesuriyachan,Dheerawan Boonyawan,최은하,Wassanai Wattanutchariya 한국물리학회 2019 Current Applied Physics Vol.19 No.9
Plasma-activated water (PAW) has been in use for the past decade in sanitization against bacteria and other microorganisms. This research study compared PAW generated by a DC positive flyback transformer (FBT) underwater plasma jet with delivery of reactive species underwater by cylindrical dielectric barrier discharge (CDBD) with a neon transformer. A Box–Behnken design was adopted as a response surface methodology (RSM) to design the experimental plan and optimize operating parameters including time, gas flow, and gas ratio. The physical responses comprise optical emission spectroscopy (OES), pH, oxidation-reduction potential (ORP), and electrical conductivity (EC). The chemical responses consist of hydrogen peroxide (H2O2) and hydroxyl radicals (OH·). The biological responses include Escherichia coli reduction and Staphylococcus aureus reduction. The optimal condition for underwater plasma jet was found to be Ar gas with a flow rate of 3 slm for 6.5 min of treatment time, which can reduce E. coli and S. aureus to 7.14 ± 0.14 and 3.10 ± 0.26 in log, respectively. Also, the optimal condition for delivery of reactive species underwater by C-DBD plasma was found to be Ar (99%): O2 (1%) gas mixture with an Ar gas flow rate of 4 slm for a treatment time of 11.5 min, which could reduce E. coli and S. aureus to 0.45 ± 0.07 and 2.45 ± 0.23 in log, respectively.
Pinpanit Boonchuay,Charin Techapun,Phisit Seesuriyachan,Thanongsak Chaiyaso 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.5
Production of xylooligosaccharides (XOs)from corncob using the thermostable endo-xylanase fromStreptomyces thermovulgaris TISTR1948 was investigatedusing KOH pretreatment, followed by enzymatic hydrolysis. The optimal reaction time for production of XOs was 12 h,after which xylobiose comprised a majority of products,and a low xylose content was observed. The optimalconditions for production of XOs were studied using acentral composite design. At an enzyme concentration of129.43 U/g of substrate, 53.80oC, and pH 6.17, the yield ofXOs reached 162.97 mg/g of substrate or 752.15 mg/g ofhemicellulose in KOH-pretreated corncob. The prebioticproperties of XOs derived from corncob were alsoinvestigated using in vitro fermentation of those XOs withthe known probiotic strains Lactobacillus casei TISTR1463,L. lactis TISTR1464, and L. plantarum TISTR1465. XOsderived from corncob were comparable to commercialXOs for an ability to enhance the growth of the specifiedprobiotic lactobacilli.