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Pinpanit Boonchuay,Charin Techapun,Phisit Seesuriyachan,Thanongsak Chaiyaso 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.5
Production of xylooligosaccharides (XOs)from corncob using the thermostable endo-xylanase fromStreptomyces thermovulgaris TISTR1948 was investigatedusing KOH pretreatment, followed by enzymatic hydrolysis. The optimal reaction time for production of XOs was 12 h,after which xylobiose comprised a majority of products,and a low xylose content was observed. The optimalconditions for production of XOs were studied using acentral composite design. At an enzyme concentration of129.43 U/g of substrate, 53.80oC, and pH 6.17, the yield ofXOs reached 162.97 mg/g of substrate or 752.15 mg/g ofhemicellulose in KOH-pretreated corncob. The prebioticproperties of XOs derived from corncob were alsoinvestigated using in vitro fermentation of those XOs withthe known probiotic strains Lactobacillus casei TISTR1463,L. lactis TISTR1464, and L. plantarum TISTR1465. XOsderived from corncob were comparable to commercialXOs for an ability to enhance the growth of the specifiedprobiotic lactobacilli.