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Advanced Imaging of Nanometer-Scale Recorded Bits on Super-Resolution Near-Field Optical Disk
Pei Lin Yang,Din Ping Tsai,Cheng Wei Lin,Chih Ching Hsu,Pei Hsin Chang,Tsung Sheng Kao,Wei Chih Lin 한국물리학회 2005 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.47 No.1
An advanced imaging technique is demonstrated for fast, non-destructive and high resolution characterizations for nanometer-scale recorded bits on a super-resolution near-field optical disk (super-RENS). For the first time, an array of individual 100 nm recorded marks is imaged and studied by using the conductive-atomic force microscopy (C-AFM) method. Discussions also include comparisons of 300 nm, 200 nm and 100 nm recorded marks on both a super-RENS disk and a commercial DVD disk, and the image results are evidence of the high carrier-to-noise ratio (CNR) value on the super-RENS disk, even though the mark size has been shrunk to less than the diffraction limit.
Chryseomicrobium deserti sp. nov., isolated from desert soil in South Korea
Lin, Pei,Yan, Zheng-Fei,Li, Chang-Tian,Kook, MooChang,Wang, Qi-Jun,Yi, Tae-Hoo Microbiology Society 2017 International journal of systematic and evolutiona Vol.67 No.10
<P>A Gram-stain positive, aerobic, non-motile, rod-shaped bacterium (THG-T1.18(T)) was isolated from desert soil. Growth occurred at 20-35 degrees C (optimum 28-30 degrees C), at pH 5-7 (optimum 7) and at 0-4% NaCl (optimum 0-1 %). Based on 16S rRNA sequence analysis, the nearest phylogenetic neighbours of strain THG-T1.18 T were identified as Chryseomicrobium amylolyticum DSM 23442(T) (96.6 %), Chryseomicrobium imtechense JCM 16573 T (96.3 %) and Chryseomicrobium aureum KACC 17219(T) (96.1 %). The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminolipids and one unidentified glycolipid. The quinone system was composed of MK-7, MK-8 and MK-6. The major fatty acids were iso C15 : 0 and anteiso C15 : 0. The type of peptidoglycan was A4b, containing of L-Orn-D-Glu. The DNA G+C content of strain THG-T1.18(T) was 50.4 mol%. DNA-DNA hybridization values between strain THG-T1.18(T) and C. amylolyticum DSM 23442(T), C. imtechense JCM 16573(T), C. aureum KACC 17219(T) were 24.7% (20.1% reciprocal analysis), 19.5% (16.1 %) and 10.4% (6.7 %) respectively. On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA-DNA hybridization data, strain THG-T1.18 T represents a novel species of the genus Chryseomicrobium, for which the name Chryseomicrobium deserti sp. nov. is proposed. The type strain is THG-T1.18(T) (= KACC 18929(T) = CCTCC AB 2016179(T)).</P>
Pei Lin,Zheng-Fei Yan,Chang-Tian Li 한국생물공학회 2020 Biotechnology and Bioprocess Engineering Vol.25 No.4
Exogenous elicitors can influence accumulation of triterpenoids by regulating FPS (farnesyl pyrophosphate synthase) in Inonotus obliquus, such as birch bark (BB), birch rhizosphere soil (BS), Frankia alni (FA), and Rhizobium indigoferae (RI). Among them, the highest yield of biomass (15.6 mg/mL) could be detected by treatment with RI at 2 μg/mL for 10 d, about 2.1-fold of the control. Results showed the significant effect of elicitors on accumulation of triterpenoids by regulating FPS expression level. RI stimulated mycelium to achieve the highest accumulation of triterpenoids about 48.2 mg/g, which was about over 4-fold of the control (9.5 mg/g). Its effect on FPS expression level was greater than that of others, which was about over 10-fold of the control. However, accumulation of triterpenoids and FPS expression level were clearly down-regulated under treatment with BS. In addition, two endogenous factors (H2O2 and NO) in I. obliquus could affect accumulation of triterpenoids by regulating FPS expression level. Effect of RI on H2O2 and NO contents were higher than that of others, about 3.5-fold of the control. The minimum value of H2O2 content was detected by treatment with BS for 10 d, which was about 0.5-fold of the control. RI that assist with CAT (catalase) and SNP (sodium nitroprusside dehydrate) could stimulate triterpenoid contents to achieve highest accumulation, which was about 5.5-fold of the control. Fluctuation of H2O2 and NO content seems to play a pivotal role in accumulation of triterpenoids by regulating FPS expression level.
Lin Pei,Yan Zheng-Fei,Chang-Tian Li 한국생물공학회 2021 Biotechnology and Bioprocess Engineering Vol.26 No.4
Silver nanoparticles (AgNPs) were successfully synthesized by different extracts of Lavandula stoechas, which have an antibacterial effect on pathogenic strains, such as Candida albicans, Pseudomonas aeruginosa, and Staphylococcus aureus. Among them, AgNPs-E4 that synthesized by EtOAc extract (E4) have greater antibacterial activity than that by other extracts. The highest inhibition zone diameter (18.6 mm) appeared in C. albicans, at a dosage of 1,000 ppm AgNPs-E4. It was identified as optimal antibacterial AgNPs, which are spherical in shape with an average size at 35.5 nm and maximum absorbance peak at 525 nm. The combined use of penicillin G and AgNPs-E4 exhibited a significant synergistic inhibition effect on the growth of C. albicans, which could produce a maximum inhibition zone of about 21.0 mm. Synergism between antibiotics and AgNPs-E4 was greater than the sum of their individual effects on pathogenic strains. Also, vancomycin, tetracycline, and AgNPs-E4 have slight or no inhibition effect on Escherichia coli. But combined use of the above antibiotics and AgNPs-E4 could exhibit antibacterial activity against E. coli, and the inhibition zone achieved 14.5 and 12.6 mm, respectively, suggesting combined use was able to realize antibacterial effect from nonexistence to pass into existence. HPLC analysis revealed polyphenols were the main compositions of EtOAc extract (E4), such as oleanolic fruit-12-en-3-ol, 19-hydroxyl wusu ester, and lanolin-8-en- 3-alcohol. These polyphenols might be particularly useful for the synthesis of antibacterial AgNPs. Therefore, AgNPs synthesized by EtOAc extract of L. stoechas have potential value as antimicrobial agents and also enhance the antibacterial activity of antibiotics by combined use.
Renin dependent hypertension caused by accessory renal arteries
Pei Lin Chan,Florence Hui Sieng Tan 대한고혈압학회 2019 Clinical Hypertension Vol.25 No.1
Background: Hypokalemia in the presence of hypertension is often attributed to primary hyperaldosteronism as a cause of secondary hypertension, however secondary hyperaldosteronism may present similarly. Accessory renal arteries are variants in the vascular anatomy which are often thought to be innocuous but in some circumstances can cause renovascular hypertension leading to secondary hyperaldosteronism. Case presentation: We report 2 cases of hypertension with secondary hyperaldosteronism associated with accessory renal arteries. Both patients presented with hypokalemia and further investigations revealed hyperaldosteronism with unsuppressed renin levels. Imaging studies showed the presence of accessory renal artery. Conclusion: Accessory renal arteries are a potential cause renovascular hypertension which can be detected via CT angiography or magnetic resonance angiography. Hormonal evaluation should be undertaken to determine whether its presence contributes to hypertension in the patient as targeted treatment such as aldosterone antagonist can be initiated. Surgical intervention or renal denervation may be considered in resistant cases.