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Zheng-Fei Yan,Pei Lin,Feng-Hua Tian,국무창,이태후,Chang-Tian Li 한국생물공학회 2016 Biotechnology and Bioprocess Engineering Vol.21 No.4
A farnesyl pyrophosphate synthase gene was cloned from Inonotus obliquus, designated IOFPS. The IOFPS cDNA contained an open reading frame (ORF) of 972 bps, encoding a protein of 324 amino acids. The deduced amino acid sequence of IOFPS revealed moderate homology with that of other fungi, and contained four conserved domains. Phylogenetic analysis showed that IOFPS belonged to the basidiomycete group. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis indicated that the IOFPS gene was successfully expressed in a yeast recombinant cell. Enzyme catalytic experiments were carried out with purified protein (IOFPS protein), which was isolated and purified from recombinant yeast cells. The special hydrolysis product (farnesol) was then detected by liquid chromatography coupled with tandem mass spectrometry (LC-MS). These results indicated that the cloned cDNA encoded a farnesyl diphosphate synthase and the IOFPS protein maintained catalytic activity in vitro.
Aeromicrobium halotolerans sp. nov., isolated from desert soil sample
Yan, Zheng-Fei,Lin, Pei,Chu, Xiao,Kook, MooChang,Li, Chang-Tian,Yi, Tae-Hoo Springer-Verlag 2016 Archives of microbiology Vol.198 No.5
<P>A Gram-positive, aerobic, and non-motile, rod-shaped actinomycete strain, designated YIM Y47(T), was isolated from soils collected from Turpan desert, China, and subjected to a polyphasic taxonomic study. Phylogenetic analysis indicated that strain YIM Y47(T) belonged to the genus Aeromicrobium. YIM Y47(T) shared highest 16S rRNA gene sequence similarities with Aeromicrobium massiliense JC14(T) (96.47 %). Growth occurs at 20-45 A degrees C (optimum at 30 A degrees C), pH 6.0-8.0 (optimum at pH 7.0), and salinities of 0-7.0 % NaCl (optimum at 4.0 %). The strain YIM Y47(T) exhibits chemotaxonomic features with menaquinone-7 (MK-7) as the predominant quinone, C-16:0, C-18:1 omega 9c and 10-methyl C-18:0 (> 10 %) as major fatty acids. The cell-wall peptidoglycan of strain YIM Y47(T) contained ll-diaminopimelic acid as the diagnostic diamino acid. The polar lipids were found to consist of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, and unknown phospholipids. The G+C content of the genomic DNA of strain YIM Y47(T) was found to be 44.7 mol%. On the basis of phylogenetic analyses and phenotypic data, it is proposed that strain YIM Y47(T) should be classified as representing a novel species of the genus Aeromicrobium, with the name Aeromicrobium halotolerans sp. nov. The type strain is YIM Y47(T) (=KCTC 39113(T)=CGMCC 1.15063(T)=DSM 29939(T)=JCM 30627(T)).</P>
Zheng-Fei Yan,Jian Guo,Feng-Hua Tian,Xin-Xin Mao,Yu Li,Chang-Tian Li 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.4
Schisandra chinensis has been used as traditional medicine. The structures of isolate active compounds (schisandrin B, deoxyschisandrin, schisandrin C) from S. chinensis were characterized by physical and spectroscopic analyses. Active compounds were tested for their potential to act as anti-melanogenesis or skin-whitening agents by their abilities to inhibit tyrosinase activity in the cell-free mushroom tyrosinase assay and cellular tyrosinase derived from B16 melanoma cells. The tyrosinase inhibitory activity was correlated to the inhibition of melanin productions in α-MSH-stimulated and unstimulated B16 cells. Cellular tyrosinase kinetics were analyzed and showed by Lineweaver- Burk plot. Schisandrin B was minimally cytotoxic (cell viability: 88.99% at 0.75 μM) and the IC50 value for suppression of mushroom tyrosinase activity was estimated as 0.6 μM. Zymography analysis demonstrated schisandrin B’s concentration-dependent effects and the kinetic analysis indicated schisandrin B’s noncompetitive-inhibitory action.