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- 저자 : Patra, N. (검색결과 157 건)
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Chini Deep Sankar,Mondal Niladri,Kar Avijit,Bunholi Ingrid,Singh Sourav,Ghosh Pratik,Patra Prasanta,Patra Shampa,Patra Bidhan Chandra 한국해양과학기술원 2023 Ocean science journal Vol.58 No.2
Marine fishes are one of the important factors in stabilizing the local aquatic ecosystem and regulating the nutritional socioeconomy of local fisher folks. The recent increases in anthropogenic activity, pollution and overfishing have led to the decline of marine fish species richness and their local aquatic habitats. In this study we sought to determine the inter-relationship between water quality, anthropogenic activity, and fish landing stations through a 31 km stretch of the East Midnapore coast in West Bengal, India which is known for its tourist destinations. The study was conducted monthly on different trawler fish landing sites from Dec 2018 to Dec 2021. During this period, we took fish samples and identified them. We obtained water quality data regarding Sea Surface Temperature (SST), Concentration of Chlorophyll-a (Chl-a), Turbidity, and Dissolved Oxygen (DO) in order to identify further correlation between the water quality analysis and species diversity. 154 numbers of commercially important marine fish species were documented. As per the IUCN database, 13% of the total fish species fall under the red list category and 16% of the species reveal a decreasing population trend. The availability of those red-listed fish throughout the season has been shown in the matrix plot to detect their gradual decrease in sighting. After analyzing the water quality data, we found out that DO, SST, Turbidity, and Chl-a correlate with the species richness on some sites and the water parameters are also differs during the seasons. Both fish species richness and water quality have been affected on those fish landing sites which have been subject to heavy anthropogenic loads.
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Green Nanobiotechnology: Factors Affecting Synthesis and Characterization Techniques
Patra, Jayanta Kumar,Baek, Kwang-Hyun Hindawi Limited 2014 Journal of nanomaterials Vol.2014 No.-
<P>Nanobiotechnology is gaining tremendous impetus in this era owing to its ability to modulate metals into their nanosize, which efficiently changes their chemical, physical, and optical properties. Accordingly, considerable attention is being given to the development of novel strategies for the synthesis of different kinds of nanoparticles of specific composition and size using biological sources. However, most of the currently available techniques are expensive, environmentally harmful, and inefficient with respect to materials and energy use. Several factors such as the method used for synthesis, pH, temperature, pressure, time, particle size, pore size, environment, and proximity greatly influence the quality and quantity of the synthesized nanoparticles and their characterization and applications. Additionally, characterization of the synthesized nanoparticles is essential to their potential use in various drug delivery and biomedical applications. The present review highlights various parameters affecting the synthesis of nanoparticles by green nanobiotechnology and different techniques used for characterizing the nanoparticles for their potential use in biomedical and environmental applications.</P>
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Tethering Meat Goats Grazing Forage of High Nutritive Value and Low to Moderate Mass
Patra, A.K.,Puchala, R.,Detweiler, G.,Dawson, L.J.,Animut, G.,Sahlu, T.,Goetsch, A.L. Asian Australasian Association of Animal Productio 2008 Animal Bioscience Vol.21 No.9
Twenty-four yearling Boer$\times$Spanish goats were used in a crossover design experiment to determine effects of tethering on forage selection, intake and digestibility, grazing behavior and energy expenditure (EE) with forage high in nutritive value and low to moderate in mass. Objectives were to determine if tethered goats could be used as a model for study of unrestrained animals and to characterize tethering as a production practice. Four 0.72-ha pastures of wheat (Triticum aestivum) and berseem clover (Trifolium alexandrium) were grazed in December and January. Each pasture hosted six animals, three with free movement and three attached to a 4.11-m tether for access to a circular area of $53.1m^2$. Tethering areas were moved each day. One animal of each treatment and pasture was used to determine forage selection, fecal output or grazing behavior and EE; therefore, there were eight observations per treatment. Mass of forage DM before grazing in Tethered areas averaged 1,280 and 1,130 kg/ha in periods 1 and 2, respectively. The CP concentration in ingesta was greater ((p<0.05) 239 and 209 g/kg; SE = 8.0) and the NDF level was lower (p<0.05) for Free vs. Tethered animals (503 and 538 g/kg; SE = 12.0); in vitro true DM digestion was similar between treatments (0.808 and 0.807 for Free and Tethered, respectively; SE = 0.0096). Intakes of DM (1,013 and 968 g/d; SE = 78.6), NDF (511 and 521 g/d; SE = 39.9) and ME (10.9 and 10.7 MJ/d; SE = 0.90) were similar between treatments, but CP intake was greater (p<0.05) for Free vs. Tethered animals (241 and 203 g/d; SE = 17.2). There were small treatment differences in in vivo apparent digestibility of OM ((p<0.05) 0.780 and 0.814; SE = 0.0049), CP ((p<0.05) 0.800 and 0.817; SE = 0.0067) and NDF ((p<0.09) 0.777 and 0.760 for Free and Tethered, respectively; SE = 0.0078). There were no treatment effects on time spent ruminating or grazing (346 and 347 min/d for Free and Tethered, respectively; SE = 42.5), but EE was considerably greater (p<0.05) for Free vs. Tethered animals (571 and 489 kJ/kg $BW^{0.75}$; SE = 8.9). In conclusion, with forage of high nutritive value and low to moderate in mass, tethering can offer a production advantage over free grazing of less energy used for activity despite similar grazing time. With forage removal considerably less than that available for grazing, effects of tethering on chemical composition of selected forage were small and less than needed to markedly affect digestion. Tethering may offer a means of studying some aspects of grazing by ruminants, but would not seem suitable for energy metabolism.
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Patra, Srikanta,Yang, Hae-Sik Korean Chemical Society 2009 Bulletin of the Korean Chemical Society Vol.30 No.7
A facile, seed-mediated preparation method of trimetallic Au@Pb@Pt core-shell nanoparticles is developed. Au nanoparticles are the template seeds onto which sequentially reduced Pb and Pt are deposited. The trimetallic core-shell structure is confirmed by UV-Vis spectroscopy, TEM and EDS analysis, and cyclic voltammetry. The trimetallic Au@Pb@Pt core-shell nanoparticles show high electrocatalytic activity for formic acid and methanol electrooxidation.
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Patra, N.,De, U.,Kim, T.H.,Lee, Y.J.,Ahn, M.Y.,Kim, N.D.,Yoon, J.H.,Choi, W.S.,Moon, H.R.,Lee, B.M.,Kim, H.S. Masson Pub. USA, Inc 2013 Biomedicine & pharmacotherapy Vol.67 No.5
Histone deacetylase (HDAC) inhibitors are a new class of anticancer agents that act by inhibiting cancer cell proliferation and inducing apoptosis in various cancer cell lines. To investigate the anticancer effect of a novel histone deacetylase (HDAC) inhibitor MHY219, its efficacy was compared to that of suberoylanilide hydroxamic acid (SAHA) in human prostate cancer cells. The anticancer effects of MHY219 on cell viability, HDAC enzyme activity, cell cycle regulation, apoptosis and other biological assays were performed. MHY219 was shown to enhance the cytotoxicity on DU145 cells (IC<SUB>50</SUB>, 0.36μM) when compared with LNCaP (IC<SUB>50</SUB>, 0.97μM) and PC3 cells (IC<SUB>50</SUB>, 5.12μM). MHY219 showed a potent inhibition of total HDAC activity when compared with SAHA. MHY219 increased histone H3 hyperacetylation and reduced the expression of class I HDACs (1, 2 and 3) in prostate cancer cells. MHY219 effectively increased the sub-G1 fraction of cells through p21 and p27 dependent pathways in DU145 cells. MHY219 significantly induced a G2/M phase arrest in DU145 and PC3 cells and arrested the cell cycle at G0/G1 phase in LNCaP cells. Furthermore, MHY219 effectively increased apoptosis in DU145 and LNCaP cells, but not PC3 cells, according to Annexin V/PI staining and Western blot analysis. These results indicate that MHY219 is a potent HDAC inhibitor that targets regulating multiple aspects of cancer cell death and might have preclinical value in human prostate cancer chemotherapy, warranting further investigation.
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Patra, Amlan Kumar Asian Australasian Association of Animal Productio 2014 Animal Bioscience Vol.27 No.4
This study presents trends and projected estimates of methane and nitrous oxide emissions from livestock of India vis-$\grave{a}$-vis world and developing countries over the period 1961 to 2010 estimated based on IPCC guidelines. World enteric methane emission (EME) increased by 54.3% (61.5 to $94.9{\times}10^9kg$ annually) from the year 1961 to 2010, and the highest annual growth rate (AGR) was noted for goat (2.0%), followed by buffalo (1.57%) and swine (1.53%). Global EME is projected to increase to $120{\times}10^9kg$ by 2050. The percentage increase in EME by Indian livestock was greater than world livestock (70.6% vs 54.3%) between the years 1961 to 2010, and AGR was highest for goat (1.91%), followed by buffalo (1.55%), swine (1.28%), sheep (1.25%) and cattle (0.70%). In India, total EME was projected to grow by $18.8{\times}10^9kg$ in 2050. Global methane emission from manure (MEM) increased from $6.81{\times}10^9kg$ in 1961 to $11.4{\times}10^9kg$ in 2010 (an increase of 67.6%), and is projected to grow to $15{\times}10^9kg$ by 2050. In India, the annual MEM increased from $0.52{\times}10^9kg$ to $1.1{\times}10^9kg$ (with an AGR of 1.57%) in this period, which could increase to $1.54{\times}10^9kg$ in 2050. Nitrous oxide emission from manure in India could be $21.4{\times}10^6kg$ in 2050 from $15.3{\times}10^6kg$ in 2010. The AGR of global GHG emissions changed a small extent (only 0.11%) from developed countries, but increased drastically (1.23%) for developing countries between the periods of 1961 to 2010. Major contributions to world GHG came from cattle (79.3%), swine (9.57%) and sheep (7.40%), and for developing countries from cattle (68.3%), buffalo (13.7%) and goat (5.4%). The increase of GHG emissions by Indian livestock was less (74% vs 82% over the period of 1961 to 2010) than the developing countries. With this trend, world GHG emissions could reach $3,520{\times}10^9kg$ $CO_2$-eq by 2050 due to animal population growth driven by increased demands for meat and dairy products in the world.
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Patra, B.N.,Bais, R.K.S.,Sharma, D.,Singh, B.P.,Prasad, R.B.,Bhushan, B. Asian Australasian Association of Animal Productio 2004 Animal Bioscience Vol.17 No.4
The study was undertaken to evaluate the effect of naked neck gene on mortality, cell mediated and humoral immune response in white plumage broiler population. The mortality of homozygous naked neck (Na/Na) broilers (11.71%) was comparatively lower than that of heterozygous naked neck (Na/na) (12.28%) and normally feathered (na/na) (13.59%) broilers. The humoral immune response was measured against (1% v/v) sheep red blood cells (SRBC) for total haemagglutinin (HA) antibody, 2-mercaptoethanol resistance (MER) or (IgG) antibody and 2-mercaptoethanol sensitive (MES) or (IgM) antibody titre on 7 days post-immunization. The titre was expressed as log2 of the highest dilution which shows complete haemagglutination. Total HA titers of Na/Na and Na/na (11.05$\pm$0.53 and 11.09$\pm$0.38) were comparatively higher than that of na/na (10.26$\pm$0.42). The MES antibody titre of Na/Na (8.50$\pm$0.53) and Na/na (7.63$\pm$0.45) broilers were significantly higher as compared to na/na (6.11$\pm$0.32) broilers. The MER titre of na/na genetic group (4.15$\pm$0.42) was significantly higher than Na/Na (2.55$\pm$0.37) and comparatively higher than Na/na (3.45$\pm$0.38) broilers. In vivo cell response to phytohaemagglutinin-P (PHA-P), measured as Foot Index (FI) in mm expressed significantly higher response in Na/na (0.473$\pm$0.05) and Na/Na (0.413$\pm$0.04) broilers as compared to na/na (0.304$\pm$0.03) broilers. The result of present study suggested that white plumage naked neck broilers had better immune response as compared to normally feathered broilers.
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Antioxidant Defense and Lipid Peroxide Level in Liver and Kidneys of Lead Exposed Rats
Patra, R.C.,Swarup, D.,Dwivedi, S.K. Asian Australasian Association of Animal Productio 2000 Animal Bioscience Vol.13 No.10
An experiment was carried out with 48 IVRI 2CQ rats 6-8 week old, weighing 50-100 g, to study the effect of lead exposure on antioxidant defense, lipid peroxide level, status of thiol groups and concentration of lead in the liver and kidneys at the end of the exposure and also after withdrawal of lead administration. Twenty four rats were given lead at a daily dose rate of 1 mg lead/2 ml of distilled water/kg body weight as lead acetate solution intraperitoneally for a period of 30 days. Another 24 control rats received 2 ml of sterile normal saline solution (0.85% NaCl)/kg body weight in an identical manner. A many-fold increase in concentration of lead was associated with a non-significant (p>0.05) decrease in the activities of superoxide dismutase (SOD) in the liver (27%) and kidneys (12%) and catalase in kidneys (22%). A significant (p<0.05) increase in lipid peroxide level was recorded in the liver (40%) compared with control values. There were significant (p<0.05) decreases in the total thiol and protein bound thiol contents in liver and an increase in non-protein bound thiol groups in the kidneys of lead exposed rats. During the 10 day observation period after withdrawal of lead administration, no significant change was observed with respect to any of the above parameters indicating that a 10 day withdrawal period was not enough for restoration of normality. It is concluded that the magnitude of response and the resultant changes in the lipid peroxide concentration, and the activities of SOD and catalase were not identical in the liver and kidneys of lead-exposed rats.
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