人蔘根 腐病發生에 미치는 病原과 線蟲의 關聯性

李敏雄,申鉉成,沈在郁 동국대학교 1985 論文集 Vol.24 No.-

4 roots of 2 year old ginseng were planted in a small pot trateing with fungal pathogens, Fusarium solani, nematodes isolated from the ginseng (Panax ginseng C.A. Meyer) cultured soil and the two mixed inoculum respectively. Rate of infestation and nematodes distributed around the harvested host plant after 40 days was investigated. To obtained results were as follows: Each group was treated with F. solani, nematodes and mixed inoculum showed higher disease index by infestation from 3.3 to 3.8 while control group was healthy showing lower disease index of 1.2. The nematodes identified were 4 genera in control group and F. Solani inoculated group. 11 genera of nematodes including Xiphinema spp were distributed in the nematodes inoculated pot, and their numbers were higher in the population. Especially Radophalus spp, Tylenchulus spp, Meloidogyne spp, Xiphinema spp and Ditylenchus spp were numerous in their population density, but Belonolimus spp and Hemicyciophora spp were lower. Fourteen genera of nematodes were distributed in the mixed inoculation pot than only the nematodes treated group. and also the population was higher in the mixed inoculation group. The more populated genera is number were Ditylenchus spp, Paratylenchus spp, Paratylenchus spp. Hemicycliophora spp, Meloidogyne spp, Helicotylenchus spp and Tylenchorhynchus spp, but Hirschmanniella spp was less in the population density.

수완진동 증후군에서 신경장해의 조기진단을 위한 객관화된 방법

윤재국,이헌,최나리,김석환,박형욱,이지호,유철인 大韓産業醫學會 2009 대한직업환경의학회지 Vol.21 No.2

목적: 통각과 진동감각 역치검사 및 수부와 수지의 악력, 그리고 수지의 운동기능검사(태평) 등과 근로자들이 호소했던 증상에 대한 HAVS의 스톡홀름 워크샵 분류와의 관련성을 통해 좀 더 객관화된 신경학적 조기검사 방법을 찾고 타당성을 검증하고자 하였다. 방법: 2000년 1월부터 2007년 12월까지 수부진동에 폭로되고 수부의 불편함을 호소하여 진동 장해에 대한 검사를 시행한 497명의 근로자를 대상으로 하였다. 일차적으로 문진을 통해 직업력에 대해 알아보고 과거력을 조사했다. 대상자들의 스톡홀름 워크샵 분류에 따른 소견을 확인하고 수부의 말초 순환기능 검사(손톱압박, 수지피부 온도), 신경기능 검사(통각과 진동각 역치), 운동기능 검사(악력, 태평) 등을 시행하였다. 다른 질환의 감별을 위한 몇 가지 이학적 검사를 실시하고 스톡홀름 워크샵 분류에 따른 검사 결과들을 분석하였다. 결과: 사용 도구는 그라인더 단독 사용이 265명 (67.3%)으로 가장 많았으며 평균 노출 연수는 14.8년이었다. 통각과 진동감각에서 스톡홀름 워크샵 분류 단계에 따른 역치가 증가하는 경향을 보였으나 통각은 양측 모두 통계적인 유의성이 없었고, 진동감각은 125와 250 Hz에서 양측 모두 유의했다(p=0.006-0.038). 수부와 수지의 악력은 단계가 올라갈수록 감소하는 경향을 보였지만 수부와 수지 모두 우측에서만 통계적으로 유의했다 (p=0.041, p<0.01, p=0.034). 태평 첫수는 단계가 올라 가면서 전반적으로 태핑 첫수가 감소하였으며 좌측에서만 통계적으로 유의했다(p=0.002-0.019). 결론: 본 연구는 수부 진동 노출자들의 수부 불편함에 대한 신경계통을 초기에 정량적으로 객관화하여 진단할 수 있는 단일 방법은 아직 없으며 일상생활의 불편함과 스톡홀름 워크샵 분류 등을 통한 주관적인 증상에 더하여 수지 통각과 진동감각 역치검사, 수부와 수지 악력검사, 운동기능검사(태핑) 등을 통합하면 초기에 객관적으로 진단하는 것이 가능할 것으로 판단된다. Objectives: In order to determine find out the best methods for a more objective detection of neurologic abnormality in early hand ann vibration syndrome(HAVS), early with analyzing the validity of each of the detection methods was analyzed. We evaluated the relationships between the sensorineural stage of Stockholm-revised vibration syndrome classification and the results of several tests. Methods: 497 workers were investigated for symptom, exposure duration, the types of tools used, and medical history from January 2000 to December 2007. Pain sense threshold, vibrotactile threshold, hand grasp force, finger grasp force, and a finger tapping frequency test were performed by the workers. Results: The grinder(67.3%) was the most commonly used tool and the mean exposure duration was 14.8 years. Although the pain sense and vibrotactile threshold level tended to increase according to sensorineural stage of the Stockholm classification, there was statistically significant difference in the vibrotactile threshold of 125, 250 Hz (P=0.006~0.038) but not in the pain sense threshold. Hand and finger grasp force tended to decrease according to the sensorineural stage of Stockholm classification and there was statistically significant difference(P=0.041, <0.001, 0.034) only on the right hand side. The tapping frequency also generally decreased according to the sensorineural stage of the Stockholm classification and there was statistically significant difference (P=0.002~0.019) only on the left hand side. Conclusions: Although there is no single standardized method that can objectively diagnose the Sensorineural component of early HAVS early, the combination of subjective symptoms, the sensorineural stage of Stockholm classification, the pain and vibrotactile threshold test, the hand and finger grasp force, and the finger agility (tapping) test can objectively detect sensorineural component of HAVS early.

전자 의료 카드를 위한 보건의료 정보 표준화 연구

박흥식,남승곤,안재억,김현의,김주한,정철규,김태진,이기한 대한의료정보학회 1998 Healthcare Informatics Research Vol.4 No.2

응급의료정보 체계를 위한 전지 의료 카드의 활용방안

박흥식,남승곤,안재억,김현의,김주한,정철규,김태진,이기한 대한의료정보학회 1998 Healthcare Informatics Research Vol.4 No.2

Research Articles : Sawdust Media Affecting the Mycelial Growth and the Fruiting Body Formation of Sparassis crispa

( Jae Min Lee ),( Ji Youn Kim ),( Kyung Dal Choi ),( Kee Don Han ),( Hyeon Hur ),( Seok Won Kim ),( Jae Ouk Shim ),( Ji Youl Lee ),( Tae Soo Lee ),( Min Woong Lee ) 한국균학회 2004 Mycobiology Vol.32 No.4

Immunogenicity of a bivalent killed thimerosal-free oral cholera vaccine, Euvichol, in an animal model

Lee, Eun Young,Lee, Sena,Rho, Semi,Kim, Jae-Ouk,Choi, Seuk Keun,Lee, Young Jin,Park, Joo Young,Song, Manki,Yang, Jae Seung 대한백신학회 2018 Clinical and Experimental Vaccine Research Vol.7 No.2

<P><B>Purpose</B></P><P>An oral cholera vaccine (OCV), Euvichol, with thimerosal (TM) as preservative, was prequalified by the World Health Organization (WHO) in 2015. In recent years, public health services and regulatory bodies recommended to eliminate TM in vaccines due to theoretical safety concerns. In this study, we examined whether TM-free Euvichol induces comparable immunogenicity to its TM-containing formulation in animal model.</P><P><B>Materials and Methods</B></P><P>To evaluate and compare the immunogenicity of the two variations of OCV, mice were immunized with TM-free or TM-containing Euvichol twice at 2-week interval by intranasal or oral route. One week after the last immunization, mice were challenged with <I>Vibrio cholerae</I> O1 and daily monitored to examine the protective immunity against cholera infection. In addition, serum samples were obtained from mice to measure vibriocidal activity and vaccine-specific IgG, IgM, and IgA antibodies using vibriocidal assay and enzyme-linked immunosorbent assay, respectively.</P><P><B>Results</B></P><P>No significant difference in immunogenicity, including vibriocidal activity and vaccine-specific IgG, IgM, and IgA in serum, was observed between mice groups administered with TM-free and -containing Euvichol, regardless of immunization route. However, intranasally immunized mice elicited higher levels of serum antibodies than those immunized via oral route. Moreover, intranasal immunization completely protected mice against <I>V. cholerae</I> challenge but not oral immunization. There was no significant difference in protection between two Euvichol variations.</P><P><B>Conclusion</B></P><P>These results suggested that TM-free Euvichol could provide comparable immunogenicity to the WHO prequalified Euvichol containing TM as it was later confirmed in a clinical study. The pulmonary mouse cholera model can be considered useful to examine <I>in vivo</I> the potency of OCVs.</P>

Effective and sustainable Cs+ remediation via exchangeable sodium-ion sites in graphene oxide fibers

Lee, Heehyeon,Lee, Kyungeun,Kim, Sang Ouk,Lee, Jae-Seung,Oh, Youngtak The Royal Society of Chemistry 2019 Journal of Materials Chemistry A Vol.7 No.30

<P>A monovalent sodium-functionalized graphene oxide fiber (Na-GO) structure was synthesized <I>via</I> facile and simple liquid coagulation of a graphene oxide solution. Supported by the stable GO framework, the readily accessible sodium site of this alkali-metal-carbon heterostructure allows effective removal of Cs<SUP>+</SUP> in aqueous medium with a rapid equilibrium time (∼30 min) and a large adsorption capacity (220 mg g<SUP>−1</SUP>). Na-GO possesses physical and chemical integrity in a broad pH range (4-10), and the adsorption behavior is influenced by the hydration radius of the targeting cation, charge effect, π-M<SUP>+</SUP> interaction, and pH-dependent GO hydrophilicity. In utilizing the chemical potential effect of Na-GO, a simple regeneration process with NaOH solution selectively releases captured Cs<SUP>+</SUP> and replenishes functional sodium sites within the Na-GO structure, providing a rechargeable Cs<SUP>+</SUP> remediation functionality. This study demonstrates the successful adaptation of the alkali-metal-induced reversible ion-exchange principle for a versatile GO fiber structure.</P>

Notes : Blue Mold of Pear Caused by Penicillium aurantiogriseum in Korea

(Jae Ouk Shim),(Kyung Dal Chai),(Ki Don Hahn),(Jang Hoon Lee),(Ik Hwa Hyun),(Tae Soo Lee),(Kyoung Il Ko),(Hai Poong Lee),(Min Woong Lee) 한국균학회 2002 Mycobiology Vol.30 No.2

Research Articles : The Fruiting Body Formation of Oudemansiella radicata in the Sawdust of Oak (Quercus variabilis) Mixed with Rice Bran

( Jae Ouk Shim ),( Kwang Choon Chang ),( Tae Hyun Kim ),( Youn Su Lee ),( U Youn Lee ),( Tae Soo Lee ),( Min Woong Lee ) 한국균학회 2006 Mycobiology Vol.34 No.1

Two distinct mechanisms of transcriptional regulation by the redox sensor YodB

Lee, Sang Jae,Lee, In-Gyun,Lee, Ki-Young,Kim, Dong-Gyun,Eun, Hyun-Jong,Yoon, Hye-Jin,Chae, Susanna,Song, Sung-Hyun,Kang, Sa-Ouk,Seo, Min-Duk,Kim, Hyoun Sook,Park, Sung Jean,Lee, Bong-Jin National Academy of Sciences 2016 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.113 No.35

<P>For bacteria, cysteine thiol groups in proteins are commonly used as thiol-based switches for redox sensing to activate specific detoxification pathways and restore the redox balance. Among the known thiol-based regulatory systems, the MarR/DUF24 family regulators have been reported to sense and respond to reactive electrophilic species, including diamide, quinones, and aldehydes, with high specificity. Here, we report that the prototypical regulator YodB of the MarR/DUF24 family from Bacillus subtilis uses two distinct pathways to regulate transcription in response to two reactive electrophilic species (diamide or methyl-p-benzoquinone), as revealed by X-ray crystallography, NMR spectroscopy, and biochemical experiments. Diamide induces structural changes in the YodB dimer by promoting the formation of disulfide bonds, whereas methyl-p-benzoquinone allows the YodB dimer to be dissociated from DNA, with little effect on the YodB dimer. The results indicate that B. subtilis may discriminate toxic quinones, such as methyl-p-benzoquinone, from diamide to efficiently manage multiple oxidative signals. These results also provide evidence that different thiol-reactive compounds induce dissimilar conformational changes in the regulator to trigger the separate regulation of target DNA. This specific control of YodB is dependent upon the type of thiol-reactive compound present, is linked to its direct transcriptional activity, and is important for the survival of B. subtilis. This study of B. subtilis YodB also provides a structural basis for the relationship that exists between the ligand-induced conformational changes adopted by the protein and its functional switch.</P>