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Effects of initial imperfections on nonlinear behaviors of thin-walled members
Ohga, M.,Takaue, A.,Shigematsu, T.,Hara, T. Techno-Press 2001 Structural Engineering and Mechanics, An Int'l Jou Vol.11 No.5
The effect of the initial imperfections on the nonlinear behaviors and ultimate strength of the thin-walled members subjected to the axial loads, obtained by the finite element stability analysis, are examined. As the initial imperfections, the bucking mode shapes of the members are adopted. The buckling mode shapes of the thin-walled members are obtained by the transfer matrix method. In the finite element stability analysis, isoparametric degenerated shell element is used, and the geometrical and material nonlinearity are considered based on the Green Lagrange strain definition and the Prandtl-Reuss stress-strain relation following the von Mises yield criterion. The U-, box- and I-section members subjected to the axial loads are adopted for numerical examples, and the effects of the initial imperfections on the nonlinear behaviors and ultimate strength of the members are examined.
Ohga, Shoji,Yoshimoto, Hiroaki,Pokhrel, Chandra,Yang, Bai-Song,Miyazawa, Noriko,Meng, Tianxiao,Hosoda, Shun,Mae, Masuo,Sun, Zhu Institute of Agricultural Science 2008 Korean Journal of Agricultural Science Vol.35 No.2
Mycelial expansion and fruit body development of caterpillar fungi, Cordyceps sobolifera(Hill.) Berk. et Br. was examined on various carbon and nitrogen sources, some substrates with various supplements. Best temperature for the mycelial expansion was $25^{\circ}C$, while optimal pH was 7. Glucose and polypeptone were the best sources of carbon and nitrogen, respectively, as well as 1:1 was the best ratio of carbon and nitrogen. The unpolish rice and bean curd lees were used as core substrates combined individually with five various supplements. In substrates based, all the supplements with all combinations were found to exhibit the mycelial growth and fruit body formation. Likewise, yolk was found as a superior supplement and also clarified quite high polysaccharide content in the fruit body.
Application of electric pulsed power on fruit body production of edible and medicinal mushrooms
Ohga Shoji 충남대학교 농업과학연구소 2012 농업과학연구 Vol.39 No.4
Effect of pulsed power was investigated on fruit body formation of 10 edible mushrooms, Lentinula edodes, Glifola frondosa, Pholiota nameko, Flammulina velutipes, Hypsizygus marmoreus, Pleurotus ostreatus, Pleurotus eryngii, Pleurotus abalonus, Agrocybe cylindracea and Sparassis crispa. Pulsed power of 100-170 kV was directly charged to the substrate just before fructification. The effect of the pulsed power resulted to promote for 10 edible mushrooms fructification. The treatment especially stimulated the fructification on Pleurotus species.
Application of electric pulsed power on fruit body production of edible and medicinal mushrooms
Ohga, Shoji Institute of Agricultural Science 2012 Korean Journal of Agricultural Science Vol.39 No.4
Effect of pulsed power was investigated on fruit body formation of 10 edible mushrooms, Lentinula edodes, Glifola frondosa, Pholiota nameko, Flammulina velutipes, Hypsizygus marmoreus, Pleurotus ostreatus, Pleurotus eryngii, Pleurotus abalonus, Agrocybe cylindracea and Sparassis crispa. Pulsed power of 100-170 kV was directly charged to the substrate just before fructification. The effect of the pulsed power resulted to promote for 10 edible mushrooms fructification. The treatment especially stimulated the fructification on Pleurotus species.
Ohga, Masahiro,Ogura, Mariko,Matsumura, Mastoshi,Wang, Pi-Chao The Korean Society for Biotechnology and Bioengine 2002 Biotechnology and Bioprocess Engineering Vol.7 No.5
Cell therapy applied to wound healing or tissue regeneration presents a revolutionary realm to which principles of gene engineering and delivery may be applied. One promising application is the transplantation of cells into the wounded tissue to help the tissue repair. However, when cells are transplanted from in vitro to in vivo, immune rejection occurs due to the immune response triggered by the activation of T-cell, and the transplanted cells are destroyed by the attack of activated T-cell and lose their function. Immune suppressant such as FK506 is commonly used to suppress immune rejection during transplantation. However, such kind of immune suppressants not only suppresses immune rejection in the periphery of transplanted cells but also suppresses whole immune response system against pathogenic infection. In order to solve this problem, we developed a method to protect the desired cells from immune rejection without impairing whole immune system during cell transplantation. Previously, we reported the success of constructing glomerular epithelial cells for removal of immune complex, in which complement receptor of type 1 (CR1) was over-expressed on the membrane of renal glomerular epithelial cells and could bind immune complex of DNA/anti-DNA-antibody to remove immune complex through phagocy-tosis [1]. Attempting to apply the CR1-expressing cells to cell therapy and evade immune rejection during cell transplantation, we constructed three plasmids containing genes encoding a soluble fusion protein of cytolytic T lymphocyte associated antigen-4 (CTLA4Ig) and an enhanced green fluorescent protein (EGFP). The plasmids were transfected to the above-mentioned glomerular epithelial cells to express both genes simultaneously. Using the clone cells for cell transplantation showed that mice with autoimmune disease prolonged their life significantly as compared with the control mice, and two injections of the cells at the beginning of two weeks resulted in remarkable survivability, whereas it requires half a year and 50 administrations of proteins purified from the same amount of cells to achieve the same effect.
Shoji Ohga,Hiroaki Yoshimoto,Chandra Pokhrel,Yang, Baisong,Noriko Miyazawa,Tianxiao Meng,Shun Hosoda,Masuo Mae,Zhu Sun 충남대학교 농업과학연구소 2008 농업과학연구 Vol.35 No.2
Mycelial expansion and fruit body development of caterpillar fungi, Cordyceps sobolifera(Hill.) Berk. et Br. was examined on various carbon and nitrogen sources, some substrates with various supplements. Best temperature for the mycelial expansion was 25℃, while optimal pH was 7. Glucose and polypeptone were the best sources of carbon and nitrogen, respectively, as well as 1:1 was the best ratio of carbon and nitrogen. The unpolish rice and bean curd lees were used as core substrates combined individually with five various supplements. In substrates based, all the supplements with all combinations were found to exhibit the mycelial growth and fruit body formation. Likewise, yolk was found as a superior supplement and also clarified quite high polysaccharide content in the fruit body.
Buckling of sandwich cylindrical shells under axial loading
Mitao Ohga,Aruna Sanjeewa Wijenayaka,James G. A. Croll 국제구조공학회 2005 Steel and Composite Structures, An International J Vol.5 No.1
Important characteristics of the previously proposed reduced stiffness method and a summery of its design curves for the buckling of the axially loaded sandwich cylindrical shells is presented. Comparison of the lower bound obtained with FEM analysis with that from the reduced stiffness analysis shows that the proposed reduced stiffness method can provide safe lower bounds for the buckling of geometrically imperfect, axially loaded sandwich cylindrical shells. One of the attractive features of the reduced stiffness elastic lower bound analysis is that it provides safe estimates of buckling loads that do not depend on the specification of the precise magnitude of the imperfection spectra. As a result, designers can readily apply this method without being worried about possible geometrical imperfections that might be generated during fabrication and construction of sandwich cylindrical shells.
Application of electric pulsed power on fruit body production of edible and medicinal mushrooms
Shoji Ohga 충남대학교 농업과학연구소 2012 농업과학연구 Vol.39 No.4
Effect of pulsed power was investigated on fruit body formation of 10 edible mushrooms, Lentinula edodes, Glifola frondosa, Pholiota nameko, Flammulina velutipes, Hypsizygus marmoreus, Pleurotus ostreatus, Pleurotus eryngii, Pleurotus abalonus, Agrocybe cylindracea and Sparassis crispa. Pulsed power of 100-170 ㎸ was directly charged to the substrate just before fructification. The effect of the pulsed power resulted to promote for 10 edible mushrooms fructification. The treatment especially stimulated the fructification on Pleurotus species.