위암에서 Helicobacter pylori cagA, vacA, iceA 유전자와 숙주 Interleukin-1β및 Interleukin-1 수용체 길항제 유전자 다형성

이성훈 ( Seong Hun Lee ),김태오 ( Tae Oh Kim ),이동현 ( Dong Hyun Lee ),박원일 ( Won Il Park ),김광하 ( Gwang Ha Kim ),허정 ( Jeong Heo ),강대환 ( Dae Hwan Kang ),송근암 ( Geun Am Song ),조몽 ( Mong Cho ) 대한내과학회 2006 대한내과학회지 Vol.71 No.1

Background: Both Helicobacter pylori (H. pylori) cagA, vacA, iceA genotype and host IL-1B/IL-1RN polymorphisms play a role in determining the clinical consequences of H. pylori infection. This study aimed to investigate whether there might be any combinations of H. pylori cagA, vacA, iceA genotype and host IL-1B/IL-1RN polymorphisms that are particularly associated with the occurrence of gastric carcinoma in Korean patients. Methods: This study population was comprised of 239 patients with H. pylori infection: 122 with gastric carcinoma and 117 with gastritis only. DNA was isolated from gastric biopsy sample and H. pylori cagA, vacA and iceA genotype were determined by PCR. IL-1B-511 polymorphisms were genotyped by PCR-RFLP and IL-1RN polymorphisms were analyzed with variable number of tandom repeat after PCR. Results: H. pylori cagA, vacA, and iceA genotype were not associated with an increased risk for gastric carcinoma. IL-1B-511*T carriers and IL-1RN*2 carriers did not show increased risk for gastric carcinoma. On combination of bacterial/host genotypes, cagA+/IL-1B-511*T carriers and cagA+/IL-1RN*2 carriers, vacA s1/IL-1B-511*T carriers, vacA s1/IL-1RN*2 carriers, vacA m1/IL-1B-511*T carriers, vacA m1/IL-1RN*2 carriers, iceA1/IL-1B-511*T carriers, iceA1/IL-1RN*2 carriers showed no increased risk of gastric carcinoma. Conclusions: Combined H. pylori cagA, vacA, iceA genotype and host IL-1B/IL-1RN polymorphisms shows no increased risk of gastric carcinoma. Therefore, it seems other endogenous or exogenous factors may play more important role in the development of gastric carcinoma in Korean.(Korean J Med 71:24-37, 2006)

Effect of high-mobility group box 1 on keratinocytes and fibroblasts

( Chan-yang Lee ),( In-hye Kang ),( Seung-min Oh ),( Jin-woo Lee ),( Young Il Kim ),( Ki-heon Jeong ) 대한피부과학회 2020 대한피부과학회 학술발표대회집 Vol.72 No.1

Background: High-mobility group protein B1 (HMGB1) is a physiological activator of immune responses. In patients with chronic inflammatory skin disorders including lupus, atopic dermatitis, and psoriasis, HMGB1 is increased in the cutaneous lesions. Objectives: This study was designed to investigate the effect of HMGB1 on keratinocytes and fibroblasts. Methods: In this study, keratinocytes and fibroblasts were exposed to narrow band ultraviolet B (NBUVB). Thereafter, release of HMGB1 were measured. Then keratinocytes and fibroblasts were treated with recombinant HMGB1 (rHMGB1) and production of inflammatory factors such as interleukin (IL)-1β, IL-6, IL-8, IL-18, chemokine (C-X-C motif) ligand (CXCL)1, CXCL2 and tumor necrosis factor (TNF)-α were examined. Results: 24h after NBUVB irradiation, the level of HMGB1 mRNA was increased in the fibroblasts but it was decreased in the keratinocytes. Extracellular level of HMGB1 was significantly increased in both keratinocytes and fibroblasts, 72h after NBUVB irradiation. After rHMGB1 treatment, proinflammatory molecule such as CXCL-1, IL-6, IL-8 and IL-18 were significantly increased in the keratinocytes and CXCL-1, IL-6 and IL-8 were significantly increased in the fibroblasts. Conclusion: HMGB1 can be released from keratinocytes and fibroblasts by external stress such as NBUVB irradiation and leads to activation of inflammatory signaling pathways in the keratinocytes and fibroblasts.

Gene therapy of intracranial glioma using interleukin 12-secreting human umbilical cord blood-derived mesenchymal stem cells.

Ryu, Chung Heon,Park, Sang-Hoon,Park, Soon A,Kim, Seong Muk,Lim, Jung Yeon,Jeong, Chang Hyun,Yoon, Wan-Soo,Oh, Won-il,Sung, Young Chul,Jeun, Sin-Soo Mary Ann Liebert 2011 Human gene therapy Vol.22 No.6

<P>Clinical trials of gene therapy using a viral delivery system for glioma have been limited. Recently, gene therapy using stem cells as the vehicles for delivery of therapeutic agents has emerged as a new treatment strategy for malignant brain tumors. In this study, we used human umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) as delivery vehicles with glioma-targeting capabilities, and modified interleukin-12 (IL-12p40N220Q; IL-12M) as a novel therapeutic gene. We also engineered UCB-MSCs to secret IL-12M (UCB-MSC-IL12M) via tetrameric cell-permeable peptide (4HP4)-mediated adenoviral transduction. We confirmed the migratory capacity of UCB-MSC-IL12M toward GL26 mouse glioma cells by an in vitro migration assay and in vivo injection of UCB-MSC-IL12M into the ipsilateral hemisphere of implanted gliomas in C57BL/6 mice. In vivo efficacy experiments showed that intratumoral injection of UCB-MSC-IL12M significantly inhibited tumor growth and prolonged the survival of glioma-bearing mice compared with control mice. Antitumor effects were associated with increased local IL-12M levels, followed by interferon-γ secretion and T-cell infiltration in intracranial gliomas, as well as antiangiogenesis. Interestingly, tumor-free mice after UCB-MSC-IL12M treatment were resistant to ipsilateral and contralateral tumor rechallenge, which was closely associated with tumor-specific long-term T-cell immunity. Thus, our results provide the rationale for designing novel experimental protocols to induce long-term antitumor immunity against intracranial gliomas using UCB-MSCs as an effective delivery vehicle for therapeutic cytokines including IL-12M.</P>

운동의 항암효과와 암 치료를 위한 보조요법으로서 운동처치

정일규(Il Gyu Jeong),오명진(Myung Jin Oh) 한국사회체육학회 2010 한국사회체육학회지 Vol.0 No.40

It has been reported that regular exercise has the effect to reduce the risk of cancer and exercise intervention help the cancer patient to be able to get through the hard treatment procedure. In this study, the author summarized the proposed mechanisms of anti-cancer and anti-fatigue effects of exercise and introduce the exercise intervention researches so for. For the proposed anti-cancer effect of exercise, three major mechanism have been suggested as follows. The first, exercise-induced reactive oxygen species(ROS) might cause the apoptosis of pre-stage of cancer cells. The second, ROS or pro-inflammatory cytokines like IL-1 and TNF-α which can be induced by exercise might activate the signaling pathway to promote the transcription of endogenous anti-oxidant enzymes such as SOD, CAT, GPX and HSP70 and in turn increase the protective capability of our body against various carcinogenic factors. The third, Regular exercise can increase the phase Ⅱ enzymes such as GST and UDP-GT associating with DNA repair system and also increase the activities of proteasome and OGG1 which have the role of eliminating the misfolding protein or oxidized base of DNA, respectively. It also has been reported that cancer-related fatigue(CRF) might be the most common and painful side effect of cancer itself and cancer treatment such as chemical and radiation therapy or transplantation surgery. The increase of pro-inflammatory cytokines during the cancer treatment has been suggested as the most plausible etiology of CRF. The anemia induced by the decreased function of erythropoiesis in red bone marrow and deregulation of HPA axis and the abnormal change of synaptic serotonin level in central nerve system might be caused by chronically increased pro-inflammatory cytokines. Many researches which have investigated the effects of exercise intervention for cancer patients and survivors suggested that exercise might be one of the most effective way to alleviate the cancer-related fatigue and prevent the change of body composition and the decrease of muscle mass and strength, cardiovascular function and improve the pain index and quality of life(QOL). Therefore we need to encourage the cancer specialist to use the exercise intervention for cancer treatment as the most evidence-based intervention and cooperate to improve the treatment effect and quality of life(QOL) of cancer patients and survivors.

Pulmonary inflammation caused by silica dioxide nanoparticles in mice via TXNIP/NLRP3 signaling pathway

Je‑Oh Lim,Je‑Won Ko,Tae‑Yang Jung,Woong‑Il Kim,So‑Won Pak,In‑Sik Shin,Won‑Kee Yun,Hyoung‑Chin Kim,Jeong‑Doo Heo,Jong‑Choon Kim 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.3

Background Silica dioxide nanoparticles (SiONPs) have been used for various medical applications, including therapeutics and imaging, and the use of SiONPs has increased gradually over the years. However, despite an increase in the use of SiONPs, not much is known about mechanism of action of SiONPs and their pulmonary toxicity. Objective The present study investigated the pulmonary toxicity of SiONPs and explored the underlying mechanism of action, primarily focusing on thioredoxin-interacting protein (TXNIP)/NOD-like receptor pyrin domain-containing 3 (NLRP3) in SiONPs-treated mice. We investigated the toxic effects of SiONPs in the lung of BALB/c mice administered 5, 10, and 20 mg/kg SiONPs for 3 days. Results Exposure to SiONPs markedly increased inflammatory cell counts, including those of neutrophils and macrophages, and levels of inflammatory mediators, such as interleukin (IL)-1β, IL-6, and tumor necrosis factor-α in a dose-dependent manner in the bronchoalveolar lavage fluid. Moreover, the inflammation was verified upon histopathological analysis. In addition, exposure to SiONPs increased the expression of TXNIP in a dose-dependent manner and, in turn, upregulated NLRP3 inflammasome proteins, which subsequently induced IL-1β production. Conclusion Collectively, exposure to SiONPs induced inflammation in the lungs of mice, which resulted in the activation of IL-1β production via the TXNIP-NLRP3 axis. Our results provide useful information on the pulmonary toxicity induced by SiONPs and provide insights into the underlying mechanism of action.

DA-9601, a standardized extract of Artemisia asiatica, blocks TNF-alpha-induced IL-8 and CCL20 production by inhibiting p38 kinase and NF-kappaB pathways in human gastric epithelial cells.

Choi, Suck-Chei,Choi, Eun-Ju,Oh, Hyun-Mee,Lee, SungGa,Lee, Jeong-Kun,Lee, Meung-Su,Shin, Yong-Il,Choi, Suck-Jun,Chae, Jeong-Ryong,Lee, Kang-Min,Lee, Won-Jung,Park, Jae-Sik,Shin, Chang-Yell,Oh, Tae-You WJG Press 2006 WORLD JOURNAL OF GASTROENTEROLOGY Vol.12 No.30

<P>To investigate whether, or how, DA-9601, which is a new gastroprotective agent, inhibits TNF-alpha-induced inflammatory signals in gastric epithelial AGS cells.</P>

Suppression of lipopolysaccharide-induced expression of inflammatory indicators in RAW 264.7 macrophage cells by extract prepared from <i>Ginkgo biloba</i> cambial meristematic cells

Jang, Sun-Hee,Lee, Eun Kyung,Lim, Min Jung,Hong, Nam Ju,Oh, Il Seok,Jin, Young Woo,Jeong, Han-Sol,Jeong, Yong-Seob,Lee, Jeong-Chae,Jang, Yong-Suk Informa Healthcare 2012 PHARMACEUTICAL BIOLOGY Vol.50 No.4

<P><I>Context</I>: <I>Ginkgo biloba</I> L. (Ginkgoaceae) leaves have been used as an herbal medicine that has a complex range of biological activities. However, when we consider that biological activity of plant extracts is highly variable according to the source, location, and harvest season, technology to obtain the natural products with homogeneity is extremely important.</P><P><I>Objective</I>: We established the technology to obtain the cambial meristematic cells (CMCs) of <I>Ginkgo biloba</I>, which were expanded <I>in vitro</I> with homogeneity through a suspension culture and then determined the anti-inflammatory activity of fractionated samples prepared from the ethanol extract of CMCs.</P><P><I>Materials and methods</I>: We determined the anti-inflammatory activity of samples using lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophage cells. Especially, influence of sample treatment on the expression of various indicators, such as nitric oxide (NO), inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2, mitogen-activated protein (MAP) kinases, transcription factor, and cytokines, involved in inflammatory activity was assessed.</P><P><I>Results</I>: A fractionated sample demonstrated 53.4% inhibition of LPS-induced NO production from the cells. Additionally, when fractionated samples were treated, iNOS and COX-2 expressions were almost completely suppressed. Fractionated samples also inhibited the phosphorylation of LPS-induced extracellular signal-regulated (ERK) and p38 MAP kinases more than 60%. IκB phosphorylation and subsequent nuclear factor (NF)-κB activation were also suppressed by fractionated samples. The expression of pro-inflammatory cytokines, IL-6 and tumor necrosis factor (TNF)-α, was significantly inhibited by the sample treatment.</P><P><I>Discussion and conclusion</I>: Fractionated samples from the ethanol extract of <I>Ginkgo biloba</I> CMCs could potentially be the source of a powerful anti-inflammatory substance.</P>

Bi-계 고온초전도 선재의 Jc 향상에 관한 연구(Ⅰ)

정대영,오영우,한일용 慶南大學校 附設 工業技術硏究所 1992 硏究論文集 Vol.10 No.-

Ag가 피복된 Bi-계 고온 초전도 선재의 임계전류밀도(Jc)향상을 위하여 분말 충진법과 아울러 압연과 소결의 혼합공정의 반복법을 사용하였다. 선재에서 높은 Jc를 얻기 위한 요인으로는 높은 고온상(Bi-2223상)분율, 방향화된 미세조직, 입계에선의 강한 결합, 미립의 비 초전도상의 균일한 분포등이다. 압연을 통하여 유된 입자의 방향적 배열화에 의하여 weak-link는 상당히 제거될 수 있었다. 본 연구에서 얻어진 가장 높은 Jc는 77K와 OT에서 6,350 A/cm²의 값이었다. In order to improve the critical current density(Jc)by obtaining well grain-aligned morphology, Ag-sheathed Bi(Pb)-Sr-Ca-Cu-O superconducting tapes were prepared using the powder-in-tube method and a repeatition of the combined process of rolling and sintering. Key factors for obtaining high Jc in the tapes are known to be high content of hegh-Tc phase(Bi-2223 phase), well grain-aligned morphology, good bonding at grain boundaries and uniform dispersion of nonsuperconducting particles. The weak-links could be considerably eliminated by directional grain-alignment induced by rolling. The highest Jc obtained in the present study was 6,350 A/cm²at 77K and OT.

공복 시 Caffeine 투여 후 일회성 운동이 흰쥐의 인슐린 및 골격근의 GLUT-4 발현에 미치는 영향

정일규,오명진,김영표,김종오,윤재석,서태범,윤진환 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.5

This study was done to investigate the effects of caffeine ingestion on the expression of GLUT-4 in rats' skeletal muscle(soleus m) and blood insulin level after 12 hr fasting condition and one hour treadmill running. Total 19 rats(Sprague-Dawley) who were 7week Did were used for experimental subjects and raised for 1week in the room controled the air temperature and relative humudity(22℃ and 50%). They were divided three groups ; 12hr fasting group(F group, n=5), fasting+exercise group(FE, n= 7) and fasting+caffeine+exercise group(FCE group, n=7). FG and FCE group performed treadmill running for one hour and the treadmill speed was 18m/min that was supposed to be moderate exercise to rats. Each Dr rats in FCE group was forced to he given the caffeine solution(5mg/kg, Sigma Chemical co.) through stainless steel tube directly to their stomachs one hour before treadmill exercise. Immediately after treadmill running, we anesthetized them with the mixed solution(Ketamin 80g/kg and Rompun 5ml/kg) to draw 3ml blood from heart four insulin analysis and then obtained the rats' soleus muscle for GLUT-4 expression. We found that FCE group showed significantly higher GLUT - expression then the other groups and FE group showed the lowest GLUT-4 expression. This rusults suggest that the endurance type of exercise in fasting condition inhibit the GLUT-4 expression in working muscle and the caffeine helped to facilitate the GLUT-4 expression during endurance type of exercise. The future studies needed to confirm the action of caffeine to increase the GLUT- 4 and the mechanism.

알코올 섭취 후 운동이 시간 경과에 따른 혈중 알코올 농도와 간 기능 효소에 미치는 영향

오명진,김종오,윤재석,정일규 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.6

This study was done in order to investigate the effects of performing the light exercise right after alcohol drinking on the changes of blood alcohol and ALT, AST concentration, All seven healthy collegiate students volunteered to two sections of experiment, Each subject drank one bottle of Soju(360ml, 75.6g ethanol) For forty minutes at the same speed of drinking with the same kind and volume of carbohydrate snacks. Each section of the study was conducted by a two-cross-over design with four days wash-out period, All volunteers performed brisk walking exercise corresponding to about 50-60%HRmax of exercise intensity For 30minutes immediately after alcohol drinking. Blood sampling was conducted by drawing about 10ml venous blood from antecubital vein at pre -alcohol drinking, and post- 1, 2, 4 hour alcohol drinking. Blood alcohol concentration reduced significantly at all time course in exercise group compared to Control group. However, the concentration of serum aspartate aminotransferase and alanine aminotransferase, the indicator of liver cell damage, showed no significant difference between two groups. In conclusion, performing light exercise such as brisk walking after alcohol drinking may somewhat help to reduce the concentration of blood alcohol Faster by influencing the alcohol metabolism.