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Mitsuhiro Obara,Tsutomu Ishimaru,Tomomi Abiko,Daisuke Fujita,Nobuya Kobayashi,Seiji Yanagihara,Yoshimichi Fukuta 한국식물생명공학회 2014 Plant biotechnology reports Vol.8 No.3
The root is the sole organ taking up water andnutrients from soils. Hence, root system architecture (RSA)is important for enhancing high-level and stable rice(Oryza sativa L.) production. However, the geneticimprovement of RSA has received less attention than yieldand yield components. Here, we aimed to identify andcharacterize quantitative trait loci (QTLs) for RSA bydetermining the maximum root length (MRL) of seedlingsgrown hydroponically under various concentrations ofNH4?. We used a total of 280 introgression lines (ILs) withan Indica-type variety IR64 genetic background, consistingof ten sibling ILs groups, to detect the QTLs. Greatervariation of MRL was found in three sibling ILs groups. Intotal, five QTLs were detected by single marker analyses:two each on chromosomes 5 and 6 and one on chromosome7. Among them, the most effective QTL was detected on asegment derived from IR69093-41-2-3-2 (YP5), which waslocalized to the long-arm of chromosome 6. The QTL,designated as qRL6.4-YP5, concerned in root elongation. MRL and total root length of a near-isogenic line (NIL) forqRL6.4-YP5 were significantly (15.2–24.6 %) higher thanthose of IR64 over a wide range of NH4? concentrations. Root number and weight of the NIL were the same as thoseof IR64. These results indicated that qRL6.4-YP5 was aconstitutive QTL for root length in response to change innitrogen concentrations. To enhance yield potential byimproving RSA, qRL6.4-YP5 might help to improve rootdevelopment in rice molecular breeding programs withmarker-assisted selection.
Adrenal Venous Sampling for Subtype Diagnosis of Primary Hyperaldosteronism
Mitsuhide Naruse,Akiyo Tanabe,Koichi Yamamoto,Hiromi Rakugi,Mitsuhiro Kometani,Takashi Yoneda,Hiroki Kobayashi,Masanori Abe,Youichi Ohno,Nobuya Inagaki,Shoichiro Izawa,Masakatsu Sone 대한내분비학회 2021 Endocrinology and metabolism Vol.36 No.5
Adrenal venous sampling (AVS) is the key procedure for lateralization of primary hyperaldosteronism (PA) before surgery. Identification of the adrenal veins using computed tomography (CT) and intraoperative cortisol assay facilitates the success of catheterization. Although administration of adrenocorticotropic hormone (ACTH) has benefits such as improving the success rate, some unilateral cases could be falsely diagnosed as bilateral. Selectivity index of 5 with ACTH stimulation to assess the selectivity of catheterization and lateralization index (LI) >4 with ACTH stimulation for unilateral diagnosis is used in many centers. Co-secretion of cortisol from the tumor potentially affects the lateralization by the LI. Patients aged <35 years with hypokalemia, marked aldosteroneexcess, and unilateral adrenal nodule on CT have a higher probability of unilateral disease. Patients with normokalemia, mild aldosterone excess, and no adrenal tumor on CT have a higher probability of bilateral disease. Although no methods have 100% specificity for subtype diagnosis that would allow bypassing AVS, prediction of the subtype should be considered when recommending AVSto patients. Methodological standardization and strict indication improve diagnostic quality of AVS. Development of non-invasiveimaging and biochemical markers will drive a paradigm shift in the clinical practice of PA.
Development of gene-specific markers for Phosphorus uptake 1(Pup1) and Pi-gene specific markers
Joong Hyoun Chin,Cheryl O. Dalid,Maria Ruby Burgos,Stephan Haefele,Matthias Wissuwa,Masdiar Bustamam,Yohei Koide,Ona Isabelita,Nobuya Kobayashi,Casiana Vera Cruz,Sigrid Heuer 한국육종학회 2010 한국육종학회 학술발표회 발표요지 Vol.42 No.1
Study for grain-filling of rice using 13C labeling flow-metabolome analysis
Okamura Masaki,Hirai Masami Yokota,Sawada Yuji,Okamoto Mami,Arai-Sanoh Yumiko,Yoshida Hiroe,Mukouyama Takehiro,Adachi Shunsuke,Fushimi Erina,Yabe Shiori,Nakagawa Hiroshi,Kobayashi Nobuya,Kondo Motohik 한국작물학회 2017 한국작물학회 학술발표대회 논문집 Vol.2017 No.-