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Yang, Cheolhee,Choi, Minseo,Kim, Jong Goo,Kim, Hanui,Muniyappan, Srinivasan,Nozawa, Shunsuke,Adachi, Shin-ichi,Henning, Robert,Kosheleva, Irina,Ihee, Hyotcherl MDPI 2018 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.19 No.11
<P>The quaternary transition between the relaxed (R) and tense (T) states of heme-binding proteins is a textbook example for the allosteric structural transition. Homodimeric hemoglobin (HbI) from <I>Scapharca inaequivalvis</I> is a useful model system for investigating the allosteric behavior because of the relatively simple quaternary structure. To understand the cooperative transition of HbI, wild-type and mutants of HbI have been studied by using time-resolved X-ray solution scattering (TRXSS), which is sensitive to the conformational changes. Herein, we review the structural dynamics of HbI investigated by TRXSS and compare the results of TRXSS with those of other techniques.</P>
Kim, Kyung Hwan,Ki, Hosung,Oang, Key Young,Nozawa, Shunsuke,Sato, Tokushi,Kim, Joonghan,Kim, Tae Kyu,Kim, Jeongho,Adachi, Shin‐,ichi,Ihee, Hyotcherl WILEY‐VCH Verlag 2013 Chemphyschem Vol.14 No.16
<P><B>Abstract</B></P><P>The mechanism of a photochemical reaction involves the formation and dissociation of various short‐lived species on ultrafast timescales and therefore its characterization requires detailed structural information on the transient species. By making use of a structurally sensitive X‐ray probe, time‐resolved X‐ray liquidography (TRXL) can directly elucidate the structures of reacting molecules in the solution phase and thus determine the comprehensive reaction mechanism with high accuracy. In this work, by performing TRXL measurements at two different wavelengths (400 and 267 nm), the reaction mechanism of I<SUB>3</SUB><SUP>−</SUP> photolysis, which changes subtly depending on the excitation wavelength, is elucidated. Upon 400 nm photoexcitation, the I<SUB>3</SUB><SUP>−</SUP> ion dissociates into I<SUB>2</SUB><SUP>−</SUP> and I. By contrast, upon 267 nm photoexcitation, the I<SUB>3</SUB><SUP>−</SUP> ion undergoes both two‐body dissociation (I<SUB>2</SUB><SUP>−</SUP>+I) and three‐body dissociation (I<SUP>−</SUP>+2I) with 7:3 molar ratio. At both excitation wavelengths, all the transient species ultimately disappear in 80 ns by recombining to form the I<SUB>3</SUB><SUP>−</SUP> ion nongeminately. In addition to the reaction dynamics of solute species, the results reveal the transient structure of the solute/solvent cage and the changes in solvent density and temperature as a function of time.</P>
Study for grain-filling of rice using 13C labeling flow-metabolome analysis
Okamura Masaki,Hirai Masami Yokota,Sawada Yuji,Okamoto Mami,Arai-Sanoh Yumiko,Yoshida Hiroe,Mukouyama Takehiro,Adachi Shunsuke,Fushimi Erina,Yabe Shiori,Nakagawa Hiroshi,Kobayashi Nobuya,Kondo Motohik 한국작물학회 2017 한국작물학회 학술발표대회 논문집 Vol.2017 No.-