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- 저자 : Nien-Hwa Linda Wang (검색결과 4 건)
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홍석빈,최재환,박한길,Nien-Hwa Linda Wang,장용근,문성용 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.56 No.-
One of highly promising ways for fucose production is to utilize the defatted residue of microalgae as thefucose source. A prerequisite for such fucose-production strategy is a robust separation process that canperform an efficient recovery of fucose in a monosugar mixture coming from the hydrolysis of thedefatted microalgal biomass. To develop such process, wefirst selected a prospective large-scaleadsorbent that had a sufficiently high selectivity between fucose and other monosugar components. Theselected adsorbent was then experimented in accordance with the principle of multiple frontal analysisin order to obtain the intrinsic parameters of the relevant monosugar components. Using the resultantparameters, the optimal design of the fucose-separation process of interest was carried out on the basis ofa simulated moving bed (SMB) technology. The validity of the designed process was investigatedfirstwith detailed model simulation, and then with a continuous fucose-separation experiment based on theself-assembled SMB equipment. The results of the SMB experiment demonstrated that the developedprocess was highly effective in continuous separation of fucose with the purity of 97.1% whilemaintaining its loss as low as 0%. It is thus expected that the results in this study can contribute to ameaningful improvement in the economical efficiencies of both a microalgae based biodiesel-productionprocess and a fucose-production process.
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박찬훈,최재환,경명옥,서승우,조성은,이경선,김풍호,Nien-Hwa Linda Wang,정상원,문성용 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.47 No.-
Xylobiose (X2) is recognized to possess great prebiotic function and to be highly favorable for applicationin food and prebiotic industries. In this study, we demonstrated that the cloned b-xylosidase of Bacilluspumilus IPO could be utilized to produce X2 via the reaction of xylose(X1)!xylobiose(X2). The use ofsuch enzyme in the X1!X2 reaction was found to give much higher X2 reaction yield and reactionefficiency, compared to those reported in the literature. Furthermore, we developed an efficientsimulated moving bed (SMB) chromatographic process that could recover X2 from the reaction outputwith nearly 100% purity and 92% recovery on a continuous-separation mode. The developed SMB processcould also recover the unreacted X1 almost completely, which leaves room for a further increase in theoverall X2 reaction yield by reusing the recovered X1 from the SMB as the reactant of the upstreamprocessing (i.e., B. pumilus IPO b-xylosidase X1!X2 reaction). The results of this study will enable ahighly economical and environmentally-friendly production of high-purity X2 from X1.
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Lee, Chung-gi,Choi, Jae-Hwan,Park, Chanhun,Wang, Nien-Hwa Linda,Mun, Sungyong Elsevier 2017 Journal of chromatography A Vol.1527 No.-
<P>The feasibility of a simulated moving bed (SMB) technology for the continuous separation of high-purity xylobiose (X2) from the output of a beta-xylosidase X1 -> X2 reaction has recently been confirmed. To ensure high economical efficiency of the X2 production method based on the use of xylose (X1) as a starting material, it is essential to accomplish the comprehensive optimization of the X2-separation SMB process in such a way that its X2 productivity can be maximized while maintaining the X2 product concentration from the SMB as high as possible in consideration of a subsequent lyophilization step. To address this issue, a suitable SMB optimization tool for the aforementioned task was prepared based on standing wave design theory. The prepared tool was then used to optimize the SMB operation parameters, column configuration, total column number, adsorbent particle size, and X2 yield while meeting the constraints on X2 purity, X2 product concentration, and pressure drop. The results showed that the use of a larger particle size caused the productivity to be limited by the constraint on X2 product concentration, and a maximum productivity was attained by choosing the particle size such that the effect of the X2-concentration limiting factor could be balanced with that of pressure-drop limiting factor. If the target level of X2 product concentration was elevated, higher productivity could be achieved by decreasing particle size, raising the level of X2 yield, and increasing the column number in the zones containing the front and rear of X2 solute band. (c) 2017 Elsevier B.V. All rights reserved.</P>
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Park, Chanhun,Choi, Jaehwan,Kyung, Myungok,Seo, Sheungwoo,Jo, Sung-Eun,Lee, Kyungsun,Kim, Pungho,Wang, Nien-Hwa Linda,Jung, Sangwon,Mun, Sungyong Elsevier 2017 Journal of industrial and engineering chemistry Vol.47 No.-
<P><B>Abstract</B></P> <P>Xylobiose (X2) is recognized to possess great prebiotic function and to be highly favorable for application in food and prebiotic industries. In this study, we demonstrated that the cloned β-xylosidase of <I>Bacillus pumilus</I> IPO could be utilized to produce X2 via the reaction of xylose(X1)→xylobiose(X2). The use of such enzyme in the X1→X2 reaction was found to give much higher X2 reaction yield and reaction efficiency, compared to those reported in the literature. Furthermore, we developed an efficient simulated moving bed (SMB) chromatographic process that could recover X2 from the reaction output with nearly 100% purity and 92% recovery on a continuous-separation mode. The developed SMB process could also recover the unreacted X1 almost completely, which leaves room for a further increase in the overall X2 reaction yield by reusing the recovered X1 from the SMB as the reactant of the upstream processing (i.e., <I>B. pumilus</I> IPO β-xylosidase X1→X2 reaction). The results of this study will enable a highly economical and environmentally-friendly production of high-purity X2 from X1.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
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