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김풍호 ( Poong Ho Kim ),박용수 ( Yong Soo Park ),박큰바위 ( Kunbawui Park ),권지영 ( Ji Young Kwon ),유홍식 ( Hong Sik Yu ),이희정 ( Hee Jung Lee ),김지회 ( Ji Hoe Kim ),이태식 ( Tae Seek Lee ) 한국수산과학회(구 한국수산학회) 2016 한국수산과학회지 Vol.49 No.4
Pre- or post-harvest processing is required to mitigate the risk of norovirus infection mediated by shellfish or seafood. We investigated the environmental resistance of human norovirus (HuNoV) under various conditions of temperature, salinity, and pH in seawater. Male-specific coliphage (MSC) was as the reference virus for all tests. At 4℃, HuNoV GII4 spiked into seawater was continually detected by RT-PCR for 35 days, regardless of salinity or pH level. It maintained nearly stable concentrations, meaning HuNoV can sustain a viral population in seawater long enough to be accumulated by shellfish and other filter feeders during winter. MSC was also stable at 4℃ although viral infectivity dropped sharply after 28 days. The effects of salinity and pH on MSC were indistinct. At 25℃ the detectable period of HuNoV GII4 by RT-PCR in seawater decreased to about one-third or half of the period at 4℃. High salinity (32 psu) and alkaline pH (8.5) were also unfavorable for sustaining HuNoV abundance at 25℃ in seawater. The resistance patterns of MSC to high temperature, high salinity, and alkaline pH were more dramatic and viral infectivity decreased over time, almost in direct proportion to experimental days. MSC was undetectable after 12 days under all salinities and pH levels at 25℃.
김풍호 ( Poong Ho Kim ),김민정 ( Min Jeong Kim ),조미라 ( Mi Ra Jo ),이두석 ( Doo Seog Lee ),송기철 ( Ki Cheol Song ),변한석 ( Han Seok Byun ),조기채 ( Kee Chae Cho ),박광재 ( Kwang Jae Park ),전제천 ( Je Cheon Jun ),윤호동 ( Ho D 한국수산과학회(구 한국수산학회) 2010 한국수산과학회지 Vol.43 No.3
김풍호 ( Poong Ho Kim ),김민지 ( Min Ji Kim ),김지혜 ( Ji Hye Kim ),이지선 ( Ji Sun Lee ),김기현 ( Ki Hyn Kim ),김현정 ( Hyeon Jeong Kim ),전유진 ( You Jin Jeon ),허민수 ( Min Soo Heu ),김진수 ( Jin Soo Kim ) 한국수산과학회 2014 한국수산과학회지 Vol.47 No.1
This study was carried out to investigate nutritional and physiologically active characterizations of sikhae and the seasoned products from the sea squirt Halocynthia roretzi. The total taste values of sikhae fermented for 4 and 5 days were 10.9 and 15.4, respectively, which was lower than for commercially seasoned sea squirts. The sikhaes contained mostly glutamic and aspartic acids. The total amino acid contents of sikhaes fermented for 4 and 5 days were 5.5 and 6.0 g/100 g, respectively, which were lower than those of commercial seasoned-sea squirts or similar. An amount of 100 g of sikhae and its seasoned products contained P, K, Mg and Fe, and these minerals, which are deemed good for our health, were at 10% above the recommended daily requirements. The functional properties of sikhae fermented for 4 and 5 days were as follows: for ACE inhibiting activity, 69 and 69.5%, respectively; for antioxidative activity, 28.9 and 29.3%, respectively; for xanthine oxidase inhibitory activity, 52.8 and 53.1%, respectively; and for α-glucosidase inhibitory activity, 2.4 and 1.4%, respectively. Antimicrobial activity of the 5 day fermented sikhae against Vibrio parahaemolyticus and Staphyloccus aureus was detected in 8 mm and in 7 mm against Escherichia coli
김풍호 ( Poong Ho Kim ),조미라 ( Mi Ra Jo ),이희정 ( Hee Jung Lee ),김경덕 ( Kyoung Duck Kim ),하광수 ( Kwang Soo Ha ),유현덕 ( Hyun Duk Yoo ),유홍식 ( Hong Sik Lee ),이두석 ( Doo Seog Lee ),윤호동 ( Ho Dong Yoon ) 한국수산과학회(구 한국수산학회) 2011 한국수산과학회지 Vol.44 No.6
In 2007, pet food contaminated with melamine caused hundreds of dogs and cats to develop renal failure all over the world. In 2008, over 294,000 infants consumed infant formula and developed kidney stones in China. Further investigation revealed that fish feed also contained melamine; this raised concerns about melamine residues in edible fish tissues, which could have caused the urinary tract stone epidemic. In Korea, catfish fed with assorted feed that included cuttlefish organs that contained melamine developed whitening syndrome and fell dead in some populations in 2008. This event raised suspicions about the toxicity of melamine and all feeds containing melamine were immediately recalled. In this study, we investigated the rates of melamine accumulation and depletion in muscle and viscera of catfish to propose proper withdrawal periods. One group of catfish was fed a commercially available diet that contained 30, 100 and 300 mg melamine per kg diet for 14 days. To investigate residual melamine contents in muscle and viscera, other experimental groups were fed a melamine free diet after being fed melamine for 7 days. The residual amount of melamine was analyzed by LC-MS/MS. The melamine concentration in muscle was estimated to be 3.7 mg/kg after 6 days of feeding with a diet containing 300 mg melamine/kg. After 2 days of culture with a melamine free diet, the residual melamine was depleted and the concentration had decreased from 1.15 mg/kg to 0.19 mg/kg in the muscle of catfish fed a diet containing 300 mg melamine/kg for 7 days. The residual amount of melamine was reduced to 0.03 mg/kg in muscle after 7 days of culture with a melamine free diet and was undetectable after a prolonged culture period of 14 days. Catfish tend to excrete melamine rapidly after oral administration and changes in body color were not observed during the short dosing period.