Prevalence of BRCA1 and BRCA2 Germline Mutations in Breast Cancer Women of Multiple Ethnic Region in Northwest China

Jianghua Ou,Tao Wu,Rolf Sijmons,Duo Ni1,Wenting Xu,Halmurat Upur 한국유방암학회 2013 Journal of breast cancer Vol.16 No.1

Purpose: The aim of this study is to further understand the status of BRCA1 and BRCA2 mutation among Chinese high-risk breast cancer patients in multiple-ethnic regions of China. Methods: A total of 79 blood samples of high-risk breast cancer patients from Xinjiang Uyghur autonomous region were analyzed by PCR-DHPLC sequencing analysis. Results: Analysis with full length of the two genes identified a total of 6 deleterious mutations (2073delA, 2394C-T [Q759X] and IVS16+1G>A in BRCA1; 1627A-T [K467X], 6873delCTCC and 9481delA in BRCA2) in this cohort. The prevalence of BRCA1/2 germline mutation was about 7.6% (6/79) in the Xinjiang multiple ethnic region of China. Among them, 3 novel deleterious mutations, 2073delA in BRCA1 (Han ethnic Chinese) and BRCA2 variants 6873delCTCC and 9481delA (both are Kazakh ethnic Chinese), were identified and they had never been reported in breast cancer information core (BIC) database before. 2394C-T (Q759X) and IVS16+1G>A, in BRCA1 and BRCA2 variants 1627A-T were previously reported in other populations but not Chinese. Among 6 of the BRCA-related tumors, three BRCA1- and one BRCA2-associated tumors were in triple negative (estrogen receptor, progesterone receptor, and HER2 negative expressed) status and exhibited a high tumor grade. So far none of these 6 deleterious mutations were reported in ethnic Han Chinese. Conclusion: BRCA germline mutation in Chinese multiple ethnicity region may exhibit different genotypes compared to ethnic Han Chinese in other regions. These differences may arise from interaction of genetic background and environmental factors.

Clinical Application of Endoscopic Inguinal Lymph Node Resection after Lipolysis and Liposuction for Vulvar Cancer

Wu, Qiang,Zhao, Yi-Bing,Sun, Zhi-Hua,Ni, Jing,Wu, Yu-Zhong,Shao, Heng-Hua,Qu, Jun-Wei,Huang, Xin-En Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.12

Aim: To examine lymph nodes obtained after lipolysis and liposuction of subcutaneous fat of the inguinal region of female vulvar cancer patients to explore the feasibility of clinical application. Methods: The field of operation was on the basis of the range of the conventional resection of inguinal lymph nodes. We injected lipolysis liquid fanwise, started liposuction after 15-20 minutes; then the subcutaneous fatty tissue was sucked out clearly by suction tube. We selected the first puncture holes located on 2-3 cm part below anterior superior spine, the others respectively being located 3cm and 6cm below the first for puncturing into the skin, imbedding a trocar to intorduce $CO_2$ gas and the specular body, and excise the lymph nodes by ultrasonic scalpel. The surgical field chamber was set with negative pressure drainage and was pressured with a soft saline bag after surgery. Results: A lacuna emerged from subcutaneous of the inguinal region after lipolysis and liposuction, with a wide fascia easily exposed at the bottom where lymph nodes could be readily excised. The number of lymph nodes of ten patients excised within the inguinal region on each side was 4-18. The excised average number of lymph nodes was 11 when we had mature technology. Conclusion: Most of adipose tissue was removed after lipolysis and liposuction of subcutaneous tissue of inguinal region, so that the included lymph nodes were exposed and easy to excise by endoscope. This surgery avoided the large incision of regular surgery of inguinal region, the results indicating that this approach is feasible and safe for used as an alternative technology.

Time-varying modal parameters identification of large flexible spacecraft using a recursive algorithm

Ni, Zhiyu,Wu, Zhigang,Wu, Shunan The Korean Society for Aeronautical and Space Scie 2016 International Journal of Aeronautical and Space Sc Vol.17 No.2

In existing identification methods for on-orbit spacecraft, such as eigensystem realization algorithm (ERA) and subspace method identification (SMI), singular value decomposition (SVD) is used frequently to estimate the modal parameters. However, these identification methods are often used to process the linear time-invariant system, and there is a lower computation efficiency using the SVD when the system order of spacecraft is high. In this study, to improve the computational efficiency in identifying time-varying modal parameters of large spacecraft, a faster recursive algorithm called fast approximated power iteration (FAPI) is employed. This approach avoids the SVD and can be provided as an alternative spacecraft identification method, and the latest modal parameters obtained can be applied for updating the controller parameters timely (e.g. the self-adaptive control problem). In numerical simulations, two large flexible spacecraft models, the Engineering Test Satellite-VIII (ETS-VIII) and Soil Moisture Active/Passive (SMAP) satellite, are established. The identification results show that this recursive algorithm can obtain the time-varying modal parameters, and the computation time is reduced significantly.

Knock-out Rice Xylanase Inhibitor Gene (OsXII)Showed Abnormal Plant Development and Conferred Resistance to the Rice Blast Fungus

Jing Ni Wu,Yi Ming Wang,Sun Tae Kim,Kyu Young Kang 한국응용생명화학회 2008 Applied Biological Chemistry (Appl Biol Chem) Vol.51 No.5

Parametric Study of Water Inrush in a Tunnel Crossing a Fault based on the “Three Zones” fault structure

Jing Wu,Xintong Wang,Li Wu,Ya-ni Lu,Yanhua Han 대한토목학회 2022 KSCE Journal of Civil Engineering Vol.26 No.8

As tunnelling progresses into the complex geological environment such as fault zones, water inrush has become one of the main geological hazards during tunnel construction. Consequently, understanding the evolution of pore pressure and flow velocity when a tunnel is excavated in a fault zone is crucial to ensure safe working conditions and reduce construction risks. In this work, based on the concept of “Three Zones” fault structure, we simulate the nonlinear water inrush process by solving the Darcy-Brinkman flow equation for the host rock and the fault zone. We examine the impacts of 1) the angle between the tunnelling direction and the fault and 2) the relative position from the tunnel face to the fault on the evolution of pore pressure and flow velocity near the tunnel face. The results show that within 5 m to 20 m ahead of the working face, pore pressure, flow velocity, and water inrush rate are the smallest when the angle is 90°. As the angle decreases, both pore pressure and flow velocity ahead of the working face increase. The pore pressure is larger when the tunnel has not reached the fault zone than when the tunnel has crossed the fault zone. Flow velocity also exhibits similar behaviour as pore pressure. With different relative positions from the tunnel working face to the fault, the closer the tunnel face to the fault, the lower the pore pressure and the larger the flow velocity ahead of the tunnel face. The largest water inrush rate occurs when the tunnel face is excavated to the center of the fault core, and the water inrush rate declines as the distance away from the fault increases. The simulation results provided a new method for simulating water inrush when a tunnel crosses a fault and could provide valuable references for the prediction of water inrush for underground projects.

Initial Position Detection of Permanent Magnet Synchronous Machines with Self-Tuning Extended State Observer

Yawei Wu,Yifan Cui,Ronggang Ni,Shuxin Nie,Xinzhen Wu 전력전자학회 2019 ICPE(ISPE)논문집 Vol.2019 No.5

The performance of the initial rotor position detection of Permanent Magnet Synchronous Machines (PMSMs) at standstill depends much on the position observer. In this paper, the Extended State Observer (ESO) is compared with the traditional Proportional Integration (PI) observer adopting the rotating high frequency voltage injection algorithm, and the observer parameter design method is derived based on the bandwidth analysis of the close-loop transfer function. Experiments are carried out on a 3 kW PMSM prototype for verifications.

Time-varying modal parameters identification of large flexible spacecraft using a recursive algorithm

Zhiyu Ni,Zhigang Wu,Shunan Wu 한국항공우주학회 2016 International Journal of Aeronautical and Space Sc Vol.17 No.2

In existing identification methods for on-orbit spacecraft, such as eigensystem realization algorithm (ERA) and subspace method identification (SMI), singular value decomposition (SVD) is used frequently to estimate the modal parameters. However, these identification methods are often used to process the linear time-invariant system, and there is a lower computation efficiency using the SVD when the system order of spacecraft is high. In this study, to improve the computational efficiency in identifying time-varying modal parameters of large spacecraft, a faster recursive algorithm called fast approximated power iteration (FAPI) is employed. This approach avoids the SVD and can be provided as an alternative spacecraft identification method, and the latest modal parameters obtained can be applied for updating the controller parameters timely (e.g. the self-adaptive control problem). In numerical simulations, two large flexible spacecraft models, the Engineering Test Satellite-VIII (ETS-VIII) and Soil Moisture Active/Passive (SMAP) satellite, are established. The identification results show that this recursive algorithm can obtain the time-varying modal parameters, and the computation time is reduced significantly.

A Comparative Study on Ternary Low-Platinum Catalysts with Various Constructions for Oxygen Reduction and Methanol Oxidation Reactions

Yan-Ni Wu,Hai-Fu Guo,Peng Hu,Xiao-Peng Xiao,Zhao-Wang Xiao,Shi-Jun Liao 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2016 NANO Vol.11 No.7

Three types of ternary low-platinum nanocatalysts, alloy PdPtIr/C, core–shell PdPt@PtIr/C and Pd@PtIr/C, have been prepared, and their catalytic behaviors toward methanol oxidation reaction (MOR)/oxygen reduction reaction (ORR) are comparatively investigated via cyclic voltammetry and chronoamperometry analysis in an acidic medium. Through a two-step colloidal technique, the synthesized core–shell structured catalyst PtPd@PtIr/C with alloy core and alloy shell show the best catalytic activity toward MOR and the best poisoning tolerance. The alloy PdPtIr/C catalyst prepared via a one-step colloidal technique exhibits the best performance toward ORR among the three catalysts. All the three catalysts are characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD) and other characterization techniques.

Vibrio parahaemolyticus cqsA controls production of quorum sensing signal molecule 3-hydroxyundecan-4-one and regulates colony morphology

Kui Wu,Yangyun Zheng,Qingping Wu,Haiying Chen,Songzhe Fu,Biao Kan,Yongyan Long,Xiansheng Ni,Junling Tu 한국미생물학회 2019 The journal of microbiology Vol.57 No.12

In order to adapt to different environments, Vibrio parahaemolyticus employed a complicated quorum sensing system to orchestrate gene expression and diverse colony morphology patterns. In this study, the function of the putative quorum sensing signal synthase gene cqsA (VPA0711 in V. parahaemolyticus strain RIMD2210633 genome) was investigated. The cloning and expression of V. parahaemolyticus cqsA in Escherichia coli system induced the production of a new quorum sensing signal that was found in its culture supernatant. The signal was purified by high performance liquid chromatography methods and determined to be 3-hydroxyundecan- 4-one by indirect and direct mass spectra assays. The deletion of cqsA in RIMD2210633 changed V. parahaemolyticus colony morphology from the classical ‘fried-egg’ shape (thick and opaque in the center, while thin and translucent in the edge) of the wild-type colony to a ‘pancake’ shape (no significant difference between the centre and the edge) of the cqsAdeleted colony. This morphological change could be restored by complementary experiment with cqsA gene or the signal extract. In addition, the expression of opaR, a well-known quorum sensing regulatory gene, could be up-regulated by cqsA deletion. Our results suggested that V. parahaemolyticus used cqsA to produce 3-hydroxyundecan-4-one signal and thereby regulated colony morphology and other quorum sensing-associated behaviors.

ASTROD I, Test of Relativity, Solar-System Measurement and G-Wave Detection

Wei-Tou Ni,Albrecht Rudiger,Etienne Samain,Stephan Schiller,Diana Shaul,Sachie Shiomi,Timothy Sumner,Chien-Jen Tang,Jinhe Tao,Pierre Touboul,Haitao Wang,Andreas Wicht,Ji Wu,Xue-Jun Wu,Yaoheng Xiong,Ch 한국물리학회 2004 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.45 No.1

ASTROD-I is a mission concept under study to realize the general concept of ASTROD (Astrodynamical Space Test of Relativity using Optical Devices). This mission concept has one spacecraft carrying a payload of a telescope, ve lasers, and a clock together with ground stations (ODSN: Optical Deep Space Network) to test the optical scheme of interferometric and pulse ranging and yet give important scientic results. The scientic goals include a better measurement of the relativistic parameters, a better sensitivity in using optical Doppler tracking method for detecting gravitational waves, and measurement of many solar system parameters more precisely. The weight of this spacecraft is estimated to be about 300-350 kg with a payload of about 100-120 kg. The spacecraft is to be launched with initial period about 290 days and to pass by Venus twice to receive gravity-assistance for achieving shorter periods. With good orbit design, after about 370 days from launch, the spacecraft will arrive at the other side of the Sun for the determination of relativistic parameters.