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Production of anthraquinone rhamnosides in Escherichia coli
Trang Thi Huyen Nguyen,Prakash Parajuli,Ramesh Prasad Pandey,Jae Kyung Sohng 한국당과학회 2018 한국당과학회 학술대회 Vol.2018 No.01
Rhamnose is naturally occurring deoxy-sugar commonly bound to other sugars in nature. It is a common glycogenic component of glycosides in many plants and microbes. Indigenously available TDP-L-rhamnose from E. coli can be conjugated to natural products using specific glycosyltransferases. Current research focus on the enhancement in production of such potential anthraquinone deoxy-sugar conjugated products through microbial engineering and biotransformation approach. A recombinant E. coli strain was designed by expressing rhamnosyltransferase and anthraquinones were fed exogenously to produce several anthraquionone rhamnose-conjugated derivatives. Among all anthraquinones; Quinizarin, 1,4-dihydroxy-9,10-anthraquinone was efficiently bio-transformed yielding high production. These anthraquinones have important applications in the field of medicine, such as inhibitory effects against human intestinal bacteria. In addition, quinizarin exhibits inhibitory effects in the aflatoxin B1 biotransformation to the corresponding 8, 9-epoxides, which is responsible for the toxic and carcinogenic effects of aflatoxins.
Biosynthesis of Rhamnosylated Anthraquinones in Escherichia coli
Trang Thi Huyen Nguyen,신희정,Ramesh Prasad Pandey,정혜진,류광경,송재경 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.3
Rhamnose is a naturally occurring deoxysugar present as a glycogenic component of plant and microbial natural products. A recombinant mutant Escherichia coli strain was developed by overexpressing genes involved in the TDP-L-rhamnose biosynthesis pathway of different bacterial strains and Saccharothrix espanaensis rhamnosyl transferase to conjugate intrinsic cytosolic TDP-L-rhamnose with anthraquinones supplemented exogenously. Among the five anthraquinones (alizarin, emodin, chrysazin, anthrarufin, and quinizarin) tested, quinizarin was biotransformed into a rhamoside derivative with the highest conversion ratio by whole cells of engineered E. coli. The quinizarin glycoside was identified by various chromatographic and spectroscopic analyses. The anti-proliferative property of the newly synthesized rhamnoside, quinizarin-4-O-α-L-rhamnoside, was assayed in various cancer cells.
Acute Myocardial Infarction in Patients With Single Coronary Artery: A Case Report
Nguyen Thi Huyen,Nguyen Ngoc Trang,Nguyen Khoi Viet,Le Thi Thuy Lien,Hoang Thi Van Hoa,Phung Bao Ngoc,Vu Dang Luu 아시아심장혈관영상의학회 2022 Cardiovascular Imaging Asia Vol.6 No.2
A single coronary artery (SCA) is a rare congenital anomaly. In most cases, it is an incidental finding on coronary angiography and has no clinical significance. However, it can cause angina, myocardial infarction, or even sudden death. Reports of SCA with acute myocardial infarction are very rare in the medical literature. This case study presents a patient with SCA from the right aortic sinus with severe stenosis in the proximal and distal part of the right coronary artery, which was detected using cardiac angiography and cardiac multidetector computed tomography (MDCT) with acute myocardial infarction.
Nguyen, Huynh Quoc,Zada, Sahib,Lai, Trang Huyen,Pham, Trang Minh,Hwang, Jin Seok,Ahmed, Mahmoud,Kim, Deok Ryong Elsevier 2019 Neuroscience Letters Vol.701 No.-
<P><B>Abstract</B></P> <P>Oxidative damage in neurons including glutamate excitotoxicity has been linked to increasing numbers of neuropathological conditions. Under these conditions, cells trigger several different cellular responses such as autophagy, apoptosis, necrosis and senescence. However, the connection between these responses is not well understood. In this study, we found that the 60-kDa BECN1 was specifically degraded to a 40-kDa fragment in hippocampal HT22 cells treated with 5 mM glutamate. Increased BECN1 cleavage was specifically associated with a decrease in cell viability under oxidative stress. Interestingly, this BECN1 cleavage was specifically inhibited by a calpain inhibitor ALLN but was not affected by other protease inhibitors. Also, the BECN1 cleavage was not detected in calpain-4-deficient cell lines. Furthermore, calpain cleaved BECN1 at a specific site between the coiled-coil domain and Bcl2 homology 3 domain, which is associated with the anti-apoptotic protein Bcl-2. Moreover, some cellular senescence markers, including β-galactosidase, p21, p27<SUP>Kip1</SUP>, p53 and p16<SUP>INK4A</SUP>, increased proportionally to those of BECN1 cleaved fragments. These results suggest that calpain-mediated BECN1 cleavage under oxidative conditions is specifically associated with cell death induced by cellular senescence.</P> <P><B>Highlights</B></P> <P> <UL> <LI> BECN1 was specifically degraded to a 40-kDa fragment accompanying with decrease of cell viability under oxidative stress. </LI> <LI> BECN1 cleavage was inhibited by calpain inhibitor but not by other protease inhibitors. </LI> <LI> Calpain cleaves BECN1 at a specific site between CCD and BH3 domains. </LI> <LI> BECN1 degradation stimulates senescence-associated cell death at the oxidative stress. </LI> </UL> </P>