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Sang-Yeon Lee,Hyun Been Choi,Mina Park,Il Soon Choi,Jieun An,Ami Kim,Eunku Kim,Nahyun Kim,Jin Hee Han,Min young Kim,Seung min Lee,Doo-Yi Oh,Bong Jik Kim,Nayoung Yi,Nayoung, K. D. Kim,Chung Lee,Woong-Y 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-
Loss-of-function variant in the gene encoding the KCNQ4 potassium channel causes autosomal dominant nonsyndromic hearing loss (DFNA2), and no effective pharmacotherapeutics have been developed to reverse channel activity impairment. Phosphatidylinositol 4,5-bisphosphate (PIP2), an obligatory phospholipid for maintaining KCNQ channel activity, confers differentialpharmacological sensitivity of channels to KCNQ openers. Through whole-exome sequencing of DFNA2 families, we identified three novel KCNQ4 variants related to diverse auditory phenotypes in the proximal C-terminus (p.Arg331Gln), the C-terminus of the S6 segment (p.Gly319Asp), and the pore region (p.Ala271_Asp272del). Potassium currents in HEK293T cells expressing each KCNQ4 variant were recorded by patch-clamp, and functional recovery by PIP2 expression or KCNQ openers was examined. In the homomeric expression setting, the three novel KCNQ4 mutant proteins lost conductance and were unresponsive to KCNQ openers or PIP2 expression. Loss of p.Arg331Gln conductance was slightly restored by a tandem concatemer channel (WT-p.R331Q), and increased PIP2 expression further increased the concatemer current to the level of the WT channel. Strikingly, an impaired homomeric p.Gly319Asp channel exhibited hyperactivity when a concatemer (WT-p.G319D), with a negative shift in the voltage dependence of activation. Correspondingly, a KCNQ inhibitor and chelation of PIP2 effectively downregulated the hyperactive WTp. G319D concatemer channel. Conversely, the pore-region variant (p.Ala271_Asp272del) was nonrescuable under any condition. Collectively, these novel KCNQ4 variants may constitute therapeutic targets that can be manipulated by the PIP2 level and KCNQregulating drugs under the physiological context of heterozygous expression. Our research contributes to the establishment of a genotype/mechanism-based therapeutic portfolio for DFNA2.
( Nayoung Kim ),( Dong-hee Lee ),( Woo Seon Choi ),( Eunbi Yi ),( Hyojeong Kim ),( Jung Min Kim ),( Hyung-seung Jin ),( Hun Sik Kim ) 생화학분자생물학회(구 한국생화학분자생물학회) 2021 BMB Reports Vol.54 No.1
Natural killer (NK) cells, key antitumor effectors of the innate immune system, are endowed with the unique ability to spontaneously eliminate cells undergoing a neoplastic transformation. Given their broad reactivity against diverse types of cancer and close association with cancer prognosis, NK cells have gained considerable attention as a promising therapeutic target for cancer immunotherapy. NK cell-based therapies have demonstrated favorable clinical efficacies in several hematological malignancies but limited success in solid tumors, thus highlighting the need to develop new therapeutic strategies to restore and optimize anti-tumor activity while preventing tumor immune escape. The current therapeutic modalities yielding encouraging results in clinical trials include the blockade of immune checkpoint receptors to overcome the immune-evasion mechanism used by tumors and the incorporation of tumor-directed chimeric antigen receptors to enhance NK cell anti-tumor specificity and activity. These observations, together with recent advances in the understanding of NK cell activation within the tumor microenvironment, will facilitate the optimal design of NK cell-based therapy against a broad range of cancers and, more desirably, refractory cancers. [BMB Reports 2021; 54(1): 44-58]
저전압에서 다결정 실리콘 TFT의 불균일한 특성을 보상한 새로운 AMOLED 구동회로
김나영(Nayoung Kim),이문석(Moonsuk Yi) 大韓電子工學會 2009 電子工學會論文誌-SD (Semiconductor and devices) Vol.46 No.8
본 논문에서는 저전압에서 다결정 실리콘(Polycrystalline Silicon: Poly-Si) 박막 트랜지스터(Thin Film Transistors: TFTs) 의 문턱전압(threshold voltage)의 불균일성을 보상한 새로운 AMOLEDs(Active Matrix Organic Light Diodes) 구동회로를 제안한다. 제안한 회로는 6개의 스위칭, 1개의 드라이빙 TFT와 1개의 저장 콘덴서로 구성되어 있으며, SPICE 시뮬레이션을 통해 구동회로의 동작을 검증하였다. 시뮬레이션 결과 5V정도의 낮은 구동 전압(VDD)에서 제안한 화소 구동회로의 OLED 출력 전류는 0.8%정도의 오차를 갖는 반면, 기본적인 구동회로의 경우 약20%정도의 오차를 갖는 것을 확인할 수 있었다. 본 논문에서 제안한 화소 구동회로는 OLED의 전류를 결정하는 driving TFT의 threshold voltage 변화에 따른 전류의 변화를 성공적으로 보상하였고, 안정화된 전류를 OLED를 흘려주어 기본적인 화소 회로가 가지고 있던 불균일화의 문제를 해결함을 알 수 있다. A new pixel circuit for Active Matrix Organic Light Emitting Diodes (AMOLEDs), based on the polycrystalline silicon thin film transistors (Poly-Si TFTs), was proposed and verified by SMART SPICE simulation. One driving and six switching TFTs and one storage capacitor were used to improve display image uniformity without any additional control signal line. The proposed pixel circuit compensates an inevitable threshold voltage variation of Poly-Si TFTs and also compensates the degradation of OLED at low power supply voltage(VDD). The simulation results show that the proposed pixel circuit successfully compensates the variation of OLED driving current within 0.8% compared with 20% of the conventional pixel circuit.
A Novel Poly-Si TFT Pixel Circuit for AMOLED to Compensate TFT Threshold Voltage Variation
Kim, Nayoung,Yi, Moonsuk TaylorFrancis 2009 MOLECULAR CRYSTALS AND LIQUID CRYSTALS - Vol.513 No.1
<P> A new pixel circuit for Active Matrix Organic Light Emitting Diodes (AMOLEDs), based on the polycrystalline silicon thin film transistors (Poly-Si TFTs), was proposed and verified by SMART SPICE simulation. One driving and four switching TFTs and one storage capacitor was used to improve display image uniformity without any additional control signal line. The proposed pixel circuit compensates an inevitable threshold voltage variation of Poly-Si TFTs and compensates for degradation of the OLED.</P>
Da Jeong Kim,Yi Jin Jeong,Yongah Jeon,Onsook Hur,Hocheol Ko,Nayoung Ro,Juhee Rhee,Hyungjin Baek,Jung Sook Sung 한국약용작물학회 2017 한국약용작물학술대회 발표집 Vol.2017 No.05
Background : To select plant resources of the possibility of development as a natural antioxidant, the antioxidant activities including total polyphenol content (TPC), 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS), 2,2-diphenyl-1-picryl-hydrazil (DPPH), ferric reducing antioxidant power (FRAP) and reducing power (RP) of perilla accessions collected from South Korea were conducted. Method and Results : A total of 18 perilla accessions by regions were selected. Two grams of dried perilla leaves were extracted with 85% ethanol and used for analysis of antioxidant activity. Antioxidant activity value was measured in a spectrophotometer. Perilla extracts showed variation in TPC ranging from 30.87 to 92.66 ㎍ GAE ㎎-1 dw. ABTS, DPPH, FRAP and RP ranged from 6.83 to 38.64 ㎍ Trolox ㎎-1 dw, 0.63 to 8.62 ㎍ ASC ㎎-1 dw, 5.05 to 17.57 ㎍ ASC ㎎-1 dw, and 4.52 to 35.69 ㎍ ASC ㎎-1 dw, respectively. TPC was high in perilla leaves of Gyeongsang-do, but other antioxidant activities were high in perilla leaves of Chungcheong-do. Cluster analysis based on antioxidant acitivities of 18 perilla accessions consist of group Ⅰ (3 accessions), Ⅱ (2 accessions), Ⅲ (5 accessions) and Ⅳ (8 accessions). Group Ⅱ characterized as higher antioxidant activities than other groups. Principal component analysis (PCA) based on antioxidant data revealed that the first two principal components (PC1 and PC2) together explained 97.78 % total variation. Conclusion : IT242410 and IT235354 of group Ⅱ showed high antioxidant activity. These resources will be useful for developing natural antioxidants.
Mitochondria-Immobilized pH-Sensitive Off–On Fluorescent Probe
Lee, Min Hee,Park, Nayoung,Yi, Chunsik,Han, Ji Hye,Hong, Ji Hye,Kim, Kwang Pyo,Kang, Dong Hoon,Sessler, Jonathan L.,Kang, Chulhun,Kim, Jong Seung American Chemical Society 2014 JOURNAL OF THE AMERICAN CHEMICAL SOCIETY - Vol.136 No.40
<P/><P>We report here a mitochondria-targetable pH-sensitive probe that allows for a quantitative measurement of mitochondrial pH changes, as well as the real-time monitoring of pH-related physiological effects in live cells. This system consists of a piperazine-linked naphthalimide as a fluorescence off–on signaling unit, a cationic triphenylphosphonium group for mitochondrial targeting, and a reactive benzyl chloride subunit for mitochondrial fixation. It operates well in a mitochondrial environment within whole cells and displays a desirable off–on fluorescence response to mitochondrial acidification. Moreover, this probe allows for the monitoring of impaired mitochondria undergoing mitophagic elimination as the result of nutrient starvation. It thus allows for the monitoring of the organelle-specific dynamics associated with the conversion between physiological and pathological states.</P>