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신나리, 최은영, 우송희 충북대학교 생활과학연구소 2012 생활과학연구논총 Vol.16 No.1
In-service education programs are crucial in order to improve and maintain professionalism of directors and teachers at child care centers. This study focused on satisfaction of professionals working with children with in-service education programs. 324 directors and 372 child care teachers responded to a questionnaire asking for their satisfaction with various components of in-serve education programs. Chi-square tests were used to identify differences in the type and location of child care centers that the participants were employed. The main findings showed that professionals working with children at child care centers were mostly satisfied with in-service education programs at moderate levels. Significant differences by types of child care centers and by regions were hardly found.
Seo, NaRi,Lee, Sung-Ho,Ju, Kyung Won,Woo, JaeMan,Kim, BongJu,Kim, SoungMin,Jahng, Jeong Won,Lee, Jong-Ho Medknow PublicationsMedia Pvt Ltd 2018 Neural regeneration research Vol.13 No.1
<P>Bone marrow-derived mesenchymal stem cells (BMSCs) have been shown to promote the regeneration of injured peripheral nerves. Pulsed electromagnetic field (PEMF) reportedly promotes the proliferation and neuronal differentiation of BMSCs. Low-frequency PEMF can induce the neuronal differentiation of BMSCs in the absence of nerve growth factors. This study was designed to investigate the effects of low-frequency PEMF pretreatment on the proliferation and function of BMSCs and the effects of low-frequency PEMF pre-treated BMSCs on the regeneration of injured peripheral nerve using <I>in vitro</I> and <I>in vivo</I> experiments. In <I>in vitro</I> experiments, quantitative DNA analysis was performed to determine the proliferation of BMSCs, and reverse transcription-polymerase chain reaction was performed to detect S100 (Schwann cell marker), glial fibrillary acidic protein (astrocyte marker), and brain-derived neurotrophic factor and nerve growth factor (neurotrophic factors) mRNA expression. In the <I>in vivo</I> experiments, rat models of crush-injured mental nerve established using clamp method were randomly injected with low-frequency PEMF pretreated BMSCs, unpretreated BMSCs or PBS at the injury site (1 × 10<SUP>6</SUP> cells). DiI-labeled BMSCs injected at the injury site were counted under the fluorescence microscope to determine cell survival. One or two weeks after cell injection, functional recovery of the injured nerve was assessed using the sensory test with von Frey filaments. Two weeks after cell injection, axonal regeneration was evaluated using histomorphometric analysis and retrograde labeling of trigeminal ganglion neurons. <I>In vitro</I> experiment results revealed that low-frequency PEMF pretreated BMSCs proliferated faster and had greater mRNA expression of growth factors than unpretreated BMSCs. <I>In vivo</I> experiment results revealed that compared with injection of unpretreated BMSCs, injection of low-frequency PEMF pretreated BMSCs led to higher myelinated axon count and axon density and more DiI-labeled neurons in the trigeminal ganglia, contributing to rapider functional recovery of injured mental nerve. These findings suggest that low-frequency PEMF pretreatment is a promising approach to enhance the efficacy of cell therapy for peripheral nerve injury repair.</P>
최우주 ( Woo-joo Choi ),김조철 ( Jocheol Kim ),신나리 ( Nari Sin ),권경도 ( Kyung-do Kwon ) 한국농업기계학회 2023 한국농업기계학회 학술발표논문집 Vol.28 No.1
노지작물에 대한 생산성 향상 및 생산비용 절감을 위해 정밀농업 기술이 고도화됨에 따라 작물 모니터링 및 실시간 관리의 중요성이 높아지고 있다. 정밀농업의 실현을 앞당기기 위해 영상처리 및 인공지능 기술을 농업에 활용하는 연구가 활발하게 진행되고 있다. 작물 재배 과정에서 잡초는 빠르게 생장해 작물의 생장을 저해하고, 병충해 피해를 확대하며, 투입자원의 손실을 유발할 수 있다. 따라서 작물과 잡초를 인식하는 기술 개발을 통해 작물 모니터링 및 잡초 제거 등에 활용이 필요한 시점이다. 본 연구에서는 대표적인 노지작물인 콩, 마늘, 배추에 대해 RGB영상 기반 작물 및 잡초 인식 알고리즘을 개발했다. 재배기간 동안 주기적으로 작물과 잡초 영상을 카메라(GoPro HERO 10, GoPro Inc., USA)를 이용하여 취득했다. 전체 영상 중 각 작물당 300장의 영상을 Annotation했으며, 학습, 검증, 테스트를 위해 각각 80%, 10%, 10%의 비율로 데이터 세트를 구성했다. 학습을 위해 다양한 분야에서 높은 성능을 보이는 인공지능 알고리즘 중 RetinaNet, Faster RCNN, YOLO v5을 활용했고, mAP50와 추론 속도 고려한 학습결과, YOLO v5 large 기반의 알고리즘이 정확도가 높게 나타났으며, 콩, 마늘, 배추에 대한 mAP50는 각각 0.67, 0.45, 0.65으로 확인됐다. 개발된 모델은 정밀농업 기술 개발에 활용될 수 있다고 판단되며, 추후 연구에서 추가 데이터 수집 및 알고리즘 최적화를 진행할 계획이다.
Seong Woo Choi,Jun Seok Ahn,Hyoung Kyu Kim,Nari Kim,Tae-Hoon Choi,Sung-Woo Park,En A Ko,Won Sun Park,Dae-Kyu Song,Jin Han 대한생리학회-대한약리학회 2011 The Korean Journal of Physiology & Pharmacology Vol.15 No.5
ATP-sensitive K<sup>+</sup> channels (K<sub>ATP</sub>) are major component of preventing ischemia-reperfusion injury. However, there is little information regarding to the expressional difference of K<sub>ATP</sub> and its function between left and right ventricles. In this study, we measured the lactate dehydrogenase release of rabbit heart slices in vitro and determined the difference of the K<sub>ATP</sub> expression at the both ventricles by measuring the level of K<sub>ATP</sub>-forming Kir6.2 (OcKir6.2) mRNA using in situ hybridization. The hearts were preconditioned with 15 min hypoxia and reoxygenated for 15 min before a hypoxic period of 60 min, followed by reoxygenation for 180 min. With hypoxic preconditioning (100% N<sub>2</sub>) with 15 min, left ventricles (LV) showed higher release of LDH comparing with right ventricles (RV). Adding K<sub>ATP</sub> blocker glibenclamide (10ՌM) prior to a hypoxic period of 60 min, hypoxic preconditioning effect of RV was more abolished than LV. With in situ hybridization, the optical density of OcKir6.2 was higher in RV. Therefore, we suggest that different K<sub>ATP</sub> expression between LV and RV is responsible for the different response to hypoxia and hypoxic preconditioning of rabbit hearts.
신나리(Nary Shin),최은영(Eun Young Choi),우송희(Song Hee Woo) 충북대학교 생활과학연구소 2012 생활과학연구논총 Vol.16 No.1
In-service education programs are crucial in order to improve and maintain professionalism of directors and teachers at child care centers. This study focused on satisfaction of professionals working with children with in-service education programs. 324 directors and 372 child care teachers responded to a questionnaire asking for their satisfaction with various components of in-serve education programs. Chi-square tests were used to identify differences in the type and location of child care centers that the participants were employed. The main findings showed that professionals working with children at child care centers were mostly satisfied with in-service education programs at moderate levels. Significant differences by types of child care centers and by regions were hardly found.