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Single exposure fabrication and manipulation of 3D hydrogel cell microcarriers
Kim, Lily Nari,Choi, Sung-Eun,Kim, Junhoi,Kim, Hyoki,Kwon, Sunghoon Royal Society of Chemistry 2011 Lab on a chip Vol.11 No.1
<P>We present a simple and high-throughput method for fabricating free-floating hydrogel cell microcarriers using single exposure UV patterning. We also demonstrate magnetic manipulation of the free-floating cell microcarriers using a magnetic nanoparticle-embedded structure for an active agitation and a solution exchange.</P> <P>Graphic Abstract</P><P>We present a simple and high-throughput method for fabricating free-floating hydrogel cell microcarriers using single exposure UV patterning. We also demonstrate magnetic manipulation of the cell microcarriers using a magnetic nanoparticle-embedded structure for an active agitation and a solution exchange. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c0lc00369g'> </P>
Nari Kim,Jin Han,Euiyong Kim,Yun-Hee Kim,Jae-Hong Sim,Soo-Cheon Kim 대한생리학회-대한약리학회 1999 The Korean Journal of Physiology & Pharmacology Vol.3 No.6
<P> The aim of the present study is to investigate the contribution of Ca<SUP>2</SUP>-activated K<SUP></SUP> (K<SUB>Ca</SUB>) channels and delayed rectifier K<SUP></SUP> (K<SUB>V</SUB>) channels to the resting membrane potential (RMP) in rabbit middle cerebral arterial smooth muscle cells. The RMP and membrane currents were recorded using the whole-cell patch configuration and single K<SUB>Ca</SUB> channel was recorded using the outside-out patch configuration. Using the pipette solution containing 0.05 mM EGTA, the RMP was 25.76⁑5.08 mV (n=12) and showed spontaneous transient hyperpolarizations (STHPs). The membrane currents showed time- and voltage-dependent outward currents with spontaneous transient outward currents (STOCs). When we recorded the membrane potential using the pipette solution containing 10 mM EGTA, the RMP was depolarized and did not show STHPs. The membrane currents showed no STOCs but only showed slowly inactivating outward currents. External TEA (1 mM) reversibly inhibited the STHPs, depolarized the RMP, reduced the membrane currents, abolished STOCs, and decreased the open probability of single K<SUB>Ca</SUB> channel. When K<SUB>V</SUB> currents were isolated, the application of 4-AP (5 mM) depolarized the RMP. The important aspect of our results is that K<SUB>Ca</SUB> channel is responsible for the generation of the STHPs in the membrane potential and plays an important role in the regulation of the RMP and K<SUB>V</SUB> channel is also responsible for the regulation of the RMP in rabbit middle cerebral arterial smooth muscle cells.
Protein Kinase C Activates ATP-sensitive Potassium Channels in Rabbit Ventricular Myocytes
Nari Kim,Jae Boum Youm,Hyun Joo,Hyungkyu Kim,Euiyong Kim,Jin Han 대한생리학회-대한약리학회 2005 The Korean Journal of Physiology & Pharmacology Vol.9 No.4
Several signal transduction pathways have been implicated in ischemic preconditioning induced by the activation of ATP-sensitive K<SUP></SUP> (K<SUB>ATP</SUB>) channels. We examined whether protein kinase C (PKC) modulated the activity of K<SUB>ATP</SUB> channels by recording K<SUB>ATP</SUB> channel currents in rabbit ventricular myocytes using patch-clamp technique and found that phorbol 12,13-didecanoate (PDD) enhanced pinacidil-induced K<SUB>ATP</SUB> channel activity in the cell-attached configuration; and this effect was prevented by bisindolylmaleimide (BIM). K<SUB>ATP</SUB> channel activity was not increased by 4α-PDD. In excised inside- out patches, PKC stimulated K<SUB>ATP</SUB> channels in the presence of 1 mM ATP, and this effect was abolished in the presence of BIM. Heat-inactivated PKC had no effect on channel activity. PKC-induced activation of K<SUB>ATP</SUB> channels was reversed by PP2A, and this effect was not detected in the presence of okadaic acid. These results suggest that PKC activates K<SUB>ATP</SUB> channels in rabbit ventricular myocytes.
Nari Kim,Jin Han,Won Gue Kim,Euiyong Kim 대한생리학회-대한약리학회 2000 The Korean Journal of Physiology & Pharmacology Vol.4 No.4
<P> The purpose of our investigation was to examine the effects of prostaglandin F<SUB>2</SUB><SUB>α</SUB> (PGF<SUB>2</SUB><SUB>α</SUB>) on membrane potentials, Ca<SUP>2</SUP>-activated K<SUP></SUP> (K<SUB>Ca</SUB>) channels, and delayed rectifier K<SUP></SUP> (K<SUB>V</SUB>) channels using the patch-clamp technique in single rabbit middle cerebral arterial smooth muscle cells. PGF<SUB>2</SUB><SUB>α</SUB> significantly hyperpolarized membrane potentials and increased outward whole-cell K<SUP></SUP> currents. PGF<SUB>2</SUB><SUB>α</SUB> increased open-state probability of K<SUB>Ca</SUB> channels without the change of the open and closed kinetics. PGF<SUB>2</SUB><SUB>α</SUB> increased the amplitudes of K<SUB>V</SUB> currents with a leftward shift of activation and inactivation curves and a decrease of activation time constant. Our results suggest that the activation of K<SUB>Ca</SUB> and K<SUB>V</SUB> channels, at least in part, may lead to attenuate or counteract vasoconstriction by PGF<SUB>2</SUB><SUB>α</SUB> in middle cerebral artery.
Kim, Young Nam,Kim, Hyoung Kyu,Warda, Mohamad,Kim, Nari,Park, Won Sun,Prince, Ab del Bary,Jeong, Dae Hoon,Lee, Dae Shim,Kim, Ki Tae,Han, Jin Wiley (John WileySons) 2007 Proteomics. Clinical applications Vol.1 No.12
<P>Preeclampsia (PE), a pregnancy-specific syndrome of hypertension, proteinuria, and other systemic disturbances, is a state of widespread endothelial dysfunction secondary to defective placentation. Morphologically, the current data displayed degenerative and apoptotic changes in the mitochondria and villous trophoblasts of preeclamptic placenta. To reveal the superimposing alterations in placental proteins that might explain the pathophysiology of PE, we performed 2-DE MALDI-TOF MS/MS proteomics analysis of differentially expressed placental proteins with placenta from eight normal and eight preeclamptic pregnancies. The identified proteins were confirmed by Western blot analysis. We also performed morphologic evaluation of preeclamptic placentas under both electron and light microscopy. The results disclosed the marked overexpression of chaperonin 60, GST, VDAC, ERp29, and cathepsin D in PE. These proteomics findings clearly suggest the possible cellular battle against mitochondria-originated oxidative stress during PE that either end up with recovery or apoptosis. These results provide a better understanding of proteomic alterations and may help in clarification of stress-related changes in preeclamptic placentas.</P>
Kim, Lily Nari,Kim, Eun-Geun,Kim, Junhoi,Choi, Sung-Eun,Park, Wook,Kwon, Sunghoon Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.11
We have developed magnetically controllable gold nanoparticles by synthesizing superparamagnetic $Fe_3O_4$ core/gold shell nanoparticles. The core/shell particles have the capability of forming gold 1D chains in the presence of an external magnetic field. Here we demonstrate dynamic and reversible self-assembly of the gold 1D chain structures in an aqueous solution without any templates or physical or chemical attachment. The spatial configuration of gold chains can be arbitrarily manipulated by controlling the direction of a magnetic field. This technique can provide arbitrary manipulation of gold 1D chains for fabrication purpose. To demonstrate this capability, we present a technique for immobilization of the gold particle chains on a glass substrate.