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Namhee Jeong(정남희),Seung-Ho Jeon(전승호),Dool-Yi Kim(김둘이),Choonseok Lee(이춘석),Hyun-Choong Ok(옥현충),Ki-Do Park(박기도),Ha-Cheol Hong(홍하철),Seung-Sik Lee(이승식),Jung-Kyung Moon(문중경),Soo-Kwon Park(박수권) 한국생명과학회 2016 생명과학회지 Vol.26 No.10
밀의 고분자 글루테닌 서브유닛[high molecular-weight glutenin subunit (HMW-GS)]은 밀가루의 성질을 결정하는데 가장 중요한 요소이며 가공적성을 나타내는데 중요한 역할을 수행한다. 우리는 Agrobacterium 동시 형질전환법을 이용하여 한국 밀 품종인 ‘조경’으로부터 밀 HMW-GS을 암호화하는 TaGlu-Ax1 유전자를 가지는 marker-free 형질전환 벼를 생산하였다. TaGlu-Ax1 유전자의 종자 특이적 발현을 위하여 밀에서 존재하는 TaGlu-Bx7 유전자의 자체 프로모터를 벡터 내에 삽입하였다. 동시 접종을 위해서 오직 TaGlu-Ax1 유전자와 hygromycin phosphotransferase II (HPTII) 저항성 유전자만으로 구성된 두 종류의 발현 카세트를 독립적으로 Agrobacterium EHA105에 도입하였고, TaGlu-Ax1와 HPTII가 도입된 각각의 EHA105 Agrobacterium을 3:1 비율로 혼합하여 벼캘러스에 접종하였다. 210개의 HPTII 저항성 형질전환체 중에서 벼 게놈에 TaGlu-Ax1과 HPTII가 모두 삽입된 20개의 형질전환 라인을 획득하였다. TaGlu-Ax1와 HPTII가 벼 게놈에 도입된 것을 Southern blot을 통해서 다시 확인하였다. 형질전환 벼 T1 세대의 종자에서 밀 TaGlu-Ax1 유전자가 전사와 번역되어 오직 TaGlu-Ax1만을 가지는 marker-free 식물체를 T1세대에서 성공적으로 선발할 수 있었다. TaGlu-Ax1 유전자가 발현되는 marker-free 형질전환 식물체는 야생형(wild type)과의 표현형 차이는 없었다. 형질전환 벼의 쌀가루의 제빵적성을 비교하였을 때 TaGlu-Ax1 유전자만이 발현되어서는 제빵적성이 더 나아지지 않았다. 그러므로 더 많은 밀 고분자 및 저분자 글루테닌, 글리아딘의 유전자의 집적과 조합이 쌀가루 가공적성을 증진시키는데 필요하다. 결론적으로 TaGlu-Ax1 marker-free 형질전환 벼는 쌀가루 가공적성을 증진시키는데 좋은 재료로 사용될 것이다. High-molecular-weight glutenin subunits (HMW-GSs) are extremely important determinants of the functional properties of wheat dough. Transgenic rice plants containing a wheat TaGlu-Ax1 gene encoding a HMG-GS were produced from the Korean wheat cultivar ‘Jokyeong’ and used to enhance the bread-making quality of rice dough using the Agrobacterium-mediated co-transformation method. Two expression cassettes with separate DNA fragments containing only TaGlu-Ax1 and hygromycin phosphotransferase II (HPTII) resistance genes were introduced separately into the Agrobacterium tumefaciens EHA105 strain for co-infection. Rice calli were infected with each EHA105 strain harboring TaGlu-Ax1 or HPTII at a 3:1 ratio of TaGlu-Ax1 and HPTII. Among 210 hygromycin-resistant T0 plants, 20 transgenic lines harboring both the TaGlu-Ax1 and HPTII genes in the rice genome were obtained. The integration of the TaGlu-Ax1 gene into the rice genome was reconfirmed by Southern blot analysis. The transcripts and proteins of the wheat TaGlu-Ax1 were stably expressed in rice T1 seeds. Finally, the marker-free plants harboring only the TaGlu-Ax1 gene were successfully screened in the T1 generation. There were no morphological differences between the wild-type and marker-free transgenic plants. The quality of only one HMW-GS (TaGlu-Ax1) was unsuitable for bread making using transgenic rice dough. Greater numbers and combinations of HMW and LMW-GSs and gliadins of wheat are required to further improve the processing qualities of rice dough. TaGlu-Ax1 marker-free transgenic plants could provide good materials to make transgenic rice with improved bread-making qualities.
Namhee Jeong,Jung-Kyung Moon,Kiwoung Yang,Hong-Sig Kim,Soon-Chun Jeong 한국작물학회 2008 한국작물학회 학술발표대회 논문집 Vol.2008 No.10
Most soybean cultivars have ovate leaflets, although a few cultivars have narrow or lanceolate leaflets. Narrow leaflet cultivars tend to have more seeds produced per pod than ovate leaflet cultivars, suggesting that the narrow leaflet trait is tightly linked to or cosegregating with the trait controlling number of seeds produced per pod (nspp). The objective of this study was to construct a high resolution map of a chromosomal region controlling narrow leaflet trait and nspp in soybean. A BC3F2 population from a cross between 'Sowonkong' and 'V94-5152' was used. Sowonkong have narrow leaflet and 4-seeded pod and V94-5152 have ovate leaflet and do not have 4-seeded pod. The plants of F2 populations showed a segregation ratio of 3:1 of ovate leaflet to narrow leaflet and then leaflet genotypes were obtained from F2:3 population of each F2 individual. The narrow leaflet-containing plants showed Sowonkong-like pod trait and the ovate leaflet-containing plants V94-5152-like pod trait. The results suggested that, in our mapping population, a single gene controls inheritance of the narrow leaflet character and the narrow leaflet trait is tightly linked to or co-segregating with the trait controlling nspp. Subsequently, we mapped the narrow leaflet locus near Sat_105, Satt270 and SM315 on soybean linkage group I that corresponds with the previously known ln locus. Work is ongoing to construct a fine molecular genetic linkage map on this MLG I region and to find a linkage relationship between ln and nspp. Our results should facilitate further elucidation for the relationship between ln/nspp and yield.
내피와 셸 분리를 통한 공유 헬멧 사용성 개선방안 연구
김남희(Namhee Kim),문광현(Gwanghyeon Moon),김동규(Donggyu Kim),김이연(Iyoun Kim),손연수(Yeonsu Son),송재현(Jaehyun Song),정주영(Jooyoung Jung) 대한기계학회 2023 대한기계학회 춘추학술대회 Vol.2023 No.11
As electric scooters became a means of transportation, safety accidents also increased. Although a shared helmet service has been started, it is difficult to use the service due to many problems such as theft and hygiene. Even if you try to use a personal helmet, it is difficult to carry due to its large size. To solve this problem, a portable helmet is a personal helmet, which reduces the weight and size, but is unlikely to provide hygiene and safety functions. We take a survey from electric scooter users to define helmet problems and present helmets that meet the socially defined requirements for riding safety helmets. The method presented in this study is expected to improve hygiene and portability for users through partial personalization by separating the shell and lining of the helmet, and to increase the helmet wearing rate of users.
사이버대학 웹기반 교양영어 프로그램에 대한 학습자들의 인식
김남희(Kim, Namhee),문도식(Moon, Dosik) 경희대학교 언어정보연구소 2010 언어연구 Vol.27 No.3
This study investigated the cyber university students‘ perceptions of a web-based general English course. Data was collected from a survey conducted to 851 students enrolled in a General English course in the 2010 spring semester. The results of the quantitative analysis revealed that the students were satisfied with the course organization, class content, the use of multimedia, and team teaching between Korean instructors and native English-speaking teachers. However, students showed negative opinions toward content difficulty, student-teacher interaction, and the evaluation method. Concerning the learning effect of the class, the findings showed that the course was helpful in terms of overall English ability and motivation in learning English where as it was not in developing reading and speaking skills. In addition, the statistical results indicated that there were significant differences in the difficulty of class and the improvement of language ability among students according to their age and educational background. Based on the results, this study provides some pedagogical implications to improve the cyber university‘s general English course.