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섬잣나무의 胚培養에 미치는 培地조성과 植物生長 調範劑의 영향
김우룡,김돌이,강호종,김영두 진주산업대학교 농업기술연구소 1992 農業技術硏究所報 Vol.5 No.-
섬잣나무의 胚로부터 器內胚培養을 통하여 온전한 植物體를 유기시키기 위해 培地의 造成과 植物 生長調節劑의 影響을 儉素하였다. 1. 基本 培地로서 木本類의 組織培養에 양호한 GD, MS. LM배지를 기본으로 하여 實驗한 結果 GD와 MS가 섬잣나무의 胚培養에 적당하였다. 2. GD, MS, LM 배지에 auxin, cytokinin으로서 0.1ppm, 0.5ppm, 0.05ppm을 添加하여 4周間 培養하였을때 주로 GD배지에서 NAA 0.1ppm, MS배지에서는 2, 4-D 0.1ppm에서 Shoot 生長이 매우 좋은 結果를 나타냈다. 3. 식물체 유기를 위해서는 Callus段階를 거치지 않고 生長하는 胚가 있는 반면 植物 生長調節劑에 따라 Callus화 되는 胚가 있는데 현재 Callus에서 體細胞 胚 유도를 위해 4개월 동안 배양하고 있는데, 이의 연구는 섬잣나무의 組織培養을 利用 大量增殖에 많은 도움을 줄 것이다. In order to induce a complete plant from embryo of Pinus Parviflora GD, MS, LM media and plant regulators such as 2, 4-D, NAA, 2, 4-D+BAP were employed for in vitro culture media. 1. The best condition for the embryo culture was GD and MS system. 2. When the addition of plant growht regulators (0.5, 0.1, 0.05ppm) in the GD, MS and LM media, generaly GD medium supplemented with 0.1ppm of NAA, MS medium supplemented with 0.1ppm of 2, 4-D showed the best result. 3. Present studies have been investigated of callus for four months, and going to keep on inducing somatic embryo in vitro embryo culture.
Kim, Hyunmi,Lee, Kyeyoon,Hwang, Hyunsik,Bhatnagar, Nikita,Kim, Dool-Yi,Yoon, In Sun,Byun, Myung-Ok,Kim, Sun Tae,Jung, Ki-Hong,Kim, Beom-Gi Oxford University Press 2014 Journal of experimental botany Vol.65 No.2
<P>Abscisic acid (ABA) is a phytohormone that plays important roles in the regulation of seed dormancy and adaptation to abiotic stresses. Previous work identified OsPYL/RCARs as functional ABA receptors regulating ABA-dependent gene expression in <I>Oryza sativa</I>. <I>OsPYL</I>/<I>RCAR</I>s thus are considered to be good candidate genes for improvement of abiotic stress tolerance in crops. This work demonstrates that the cytosolic ABA receptor OsPYL/RCAR5 in <I>O. sativa</I> functions as a positive regulator of abiotic stress-responsive gene expression. The constitutive expression of <I>OsPYL/RCAR5</I> in rice driven by the <I>Zea mays ubiquitin</I> promoter induced the expression of many stress-responsive genes even under normal growth conditions and resulted in improved drought and salt stress tolerance in rice. However, it slightly reduced plant height under paddy field conditions and severely reduced total seed yield. This suggests that, although exogenous expression of OsPYL/RCAR5 is able to improve abiotic stress tolerance in rice, fine regulation of its expression will be required to avoid deleterious effects on agricultural traits.</P>
Expression Pattern of Potato (Solanum tuberosum) Genes under Cold Stress by using cDNA Microarray
Kim, Dool-Yi,Lee, Hye-Eun,Yi, Ki-Wan,Han, Sang-Eun,Kwon, Hawk-Bin,Go, Seung-Joo,Byun, Myung-Ok 한국유전학회 2003 Genes & Genomics Vol.25 No.4
Exploring the tolerant mechanism of crop plants to abiotic stresses causing decreased yield is one important research undertaking. The first step to achieve this goal is through isolation of genes that are regulated by such stresses. This study attempted to isolate genes that respond to cold temperature, one of the major abiotic stresses of crops. First, cDNA library with RNA extracted from vegetative leaf, stem, and root of potato plants treated at 4˚C and 25˚C for 8 hrs was constructed. To evaluate transcriptional regulation by abiotic stresses like cold (4˚C), salt (250 mM NaCl), drought (air drying for 24 hrs), and pathogen treatment (zoospore treatment of Phytophthora infestans), 100 genes were spotted onto DNA chip. Results showed that seven clones of cold stress protein were up-regulated by cold treatment, while six clones of ADP-ribosylation factor-like protein were up-regulated by salt treatment. Pathogen up-regulated the expression of ubiquitin carrier and drought stress up-regulated the expressions of cysteine protease and photosystem Ⅱ homologue. Expression of some clones showing up-regulation by cold treatment in cDNA microarray was compared with that of Northern blot analysis. Although the results of the microarray analysis were not completely consistent with that of the Northern blot analysis, there was a very strong relationship between the two analyses.
Kim, Dool-Yi,Kwon, Hawk-Bin,Lee, Hye-Eun,Jeong, Mi-Jeong,Go, Seung-Joo,Byun, Myung-Ok 한국유전학회 2004 Genes & Genomics Vol.26 No.3
Changes of expression in cold-regulated mRNA levels in potato (Solanum tuberosum L. cv. Superior) were analyzed. A total of 12,000 cDNAs was subjected to reverse Northern blot analysis using ^(32)P-dCTP- labeled first strand cDNA generated from the total RNA isolated from 25℃- and 4℃-treated potato plants. A total of 245 cDNA clones were sequenced from a cDNA library constructed from the cold treatment. Based on the BLAST results, 103 cDNA clones were found to be redundant. The analyzed genes were classified into 12 groups according to their putative functions, where the 20.2 % of group I was associated with energy metabolism, 13.1% of group XI with cell rescue and defense, 2.6% of group Ⅷwith signal transduction. Among the cDNA clones up-regulated by cold treatment from the results of the reverse Northern blot analysis, 32 were used for the Northern blot analysis. Most of the cold-treated clones showed overexpression compared with the control, while some showed down-regulation. In general, it was found that cold stress related genes were overexpressed more than two-folds at 4℃ treatment.
The Genes Associated with Drought Tolerance by Multi-Layer Approach in Potato
( Chang-kug Kim ),( Jae-hyeon Oh ),( Jong-kuk Na ),( Chuloh Cho ),( Kyung-hwa Kim ),( Go Eun Yu ),( Dool-yi Kim ) 한국육종학회 2019 Plant Breeding and Biotechnology Vol.7 No.4
We have developed a multi-layer pipeline that uses transcriptomic data to identify candidate genes associated with complex pathways in plants. We used this multi-layer approach, incorporating microarray, RNA sequencing, polymerase chain reaction (PCR), and protein-protein interaction analyses, to identify 14 genes associated with drought tolerance in potato. We generated transgenic potato lines that over-express bZIP28, one of the genes selected by our pipeline, to confirm the involvement of that gene in drought tolerance. The protein interactions of the products of the screened genes were assessed using yeast two-hybrid and bimolecular fluorescence complementation analyses. Overall, this study demonstrated the utility of our multi-layer approach for deciphering complex biosynthetic pathways using transcriptomic data.
[PE-0016] Characterization of salt tolerance cultivar(MYIR-S986) in silage rice
Kyung Hwa Kim(Kyung Hwa Kim),Soo Kwon Park(Soo Kwon Park),Mi-Suk Seo(Mi-Suk Seo),Yu Na Kim(Yu Na Kim),Jung Kyung Moon(Jung Kyung Moon),Sang Beom Lee(Sang Beom Lee),Gyu Tae Park(Gyu Tae Park),Dool-Yi K 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-
Anti-Depressant Effects of CPAE via Inhibition of cAMP Activity of 5-HT<SUB>6</SUB>
Junkee Hong,Inae Kwak,Dool-Ri Oh,Kyo-Nyeo Oh,Yujin Kim,Hyunmi Lee,Jaeyong Kim,Joohyun Oh,Haneul Jo,Chansung Park,Jimin Kim,Bomi Kim,Yongwook Lee,Chulyung Choi,Kwontaek Yi 한국식품영양과학회 2017 한국식품영양과학회 학술대회발표집 Vol.2016 No.10