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      • KCI등재

        Use of serology and polymerase chain reaction to detect atypical respiratory pathogens during acute exacerbation of chronic obstructive pulmonary disease

        ( Chi Young Jung ),( Yeoung Hun Choe ),( Sang Yeub Lee ),( Woo Jin Kim ),( Jong Deog Lee ),( Seung Won Ra ),( Eu Gene Choi ),( Jae Seung Lee ),( Myung Jae Park ),( Ju Ock Na ) 대한내과학회 2018 The Korean Journal of Internal Medicine Vol.33 No.5

        Background/Aims: To use serological and multiplex polymerase chain reaction (PCR) assays to examine sputum samples from patients experiencing acute exacerbation of chronic obstructive pulmonary disease (AECOPD) for the presence of atypical pathogens, including Mycoplasma pneumoniae, Chlamydia pneumoniae, and Legionella pneumophila. Methods: From September 2012 to February 2014, 341 patients with AECOPD attending outpatient clinics were enrolled as part of a randomized, double-blind, multicenter study. A commercial enzyme-linked immunosorbent assay was used to measure serum immunoglobulin M (IgM) and IgG antibody titers on the first day of the study and at 36 days post-enrollment. Multiplex PCR was used to test sputum samples for the presence of atypical pathogens. A urinary antigen test for L. pneumophila was performed on the first day. Results: Nineteen patients (5.6%) showed serological evidence of acute infection with M. pneumoniae. Also, one and seven patients (2%) showed serological evidence of acute infection with C. pneumoniae and L. pneumophila, respectively. All DNA samples were negative for M. pneumoniae, C. pneumoniae, and L. pneumophila according to PCR. Only one urine sample was positive for L. pneumophila antigen, but serologic evidence was lacking. Conclusions: Serological testing suggested that infection by atypical pathogens during AECOPD was relatively uncommon. In addition, PCR provided no direct evidence of infection by atypical pathogens. Thus, atypical pathogens may not be a major cause of AECOPD in South Korea.

      • SCIESCOPUSKCI등재

        Life Cycle and Predation Potential of the Black Cricket, Methicoche Vittaticollis(Orthoptera: Gryllidae) and Its Population Density in the Rice Field

        Lee, Geon-Hwi,K. L. Heong,Paik, Chae-Hoon,Kim, Doo-Ho,Na, Seung-Yeoung,Park, Man-Young Korean Society of Applied Entomology 2002 Journal of Asia-Pacific Entomology Vol.5 No.2

        Life cycle and predation potential of a predatory black crickets, Methioche vittaticollis (Stal), were studied in the laboratory at the conditions of 27$\pm\;1^{\cir}$ and 70$\pm$5% RH with a photoperiod of 14. 10 (L: D). Average life cycle was 90 days. The cylindrical eggs were laid singly within the leaf sheath and hatched in 17 days. Total nymphal period lasted 33 days. There were four nymphal stadia that lasted for 7.2, 7.7, 8.7, and 9.0 days, respectively. Adult male and female lived an average of 35 and 43 days, respectively. The female lay an average of 45 eggs during her life span. The number of striped stemborer (SSB) eggs consumed per day by 1 st, 2nd, 3rd and 4th instar nymphs of M. vittaticollis was 1.7, 2.5, 13.3 and 184.3, respectively, Also, The number of SSB eggs consumed per day by male and female adults were 102.7 and 158.7, respectively. The fourth instar nymph consumed the most number of preys. The functional response curve of 4th instar nymph, male and female adults of M. vittaticollis to the densities of SSB eggs indicated Helling's TYPE B. The consumption of prey by the M. vittaticollis increased with the prey density but the consumption rate decreased. The attack rate of 4th instar of M. vittaticollis was the highest compared to the adults, and handling time was the shortest. Population densities of hoppers and lepidoptera in unsprayed field consisted of rice variety "PSBRC 20" were very low throughout the cropping period. This could be due to the high density of if vittaticollis. M. vittaticollis was collected from the field and bunds, its population inside the field increased as crop matures. Bunds served as refuge for predators when the crop is maturity stage.ity stage.

      • KCI등재

        mtDNA D-loop 변이로 확인된 한국재래닭의 다양한 모계기원

        조창연(Chang-Yeon Cho),이풍연(Pung-Yeon Lee),고응규(Yeoung-Gyu Ko),김학규(Hak Kyu Kim),박미나(Mi-Na Park),연성흠(Seung-Hum Yeon) 韓國家禽學會 2011 韓國家禽學會誌 Vol.38 No.1

        우리나라 재래닭의 기원을 구명하기 위해서 mtDNA Dloop 영역을 분석한 결과, 1,231~1,232개의 염기구성되어 있으며, 35개소에서 변이가 관찰되었다. 변이 부위를 이용하여 Haplotype을 분류한 결과, 21종으로 분류되었으며 이중 9개인 GenBank에 미등록된 것으로 밝혀졌다. Hplotype 다양성으로 추정한 한국 재래닭의 유전적 변이성은 중국의 재래닭과 유사한 것으로 추정되었다. Haplotype에 대한 Network 분석 결과, 재래닭은 5개의 Clade로 분류되었다. 이들 Clade에 대한 각 집단의 분포 현황으로 한국 재래닭은 운남성 및 중국 재래닭이 보인 결과와 유사하나, 일본의 재래닭과는 약간 상이한 것으로 밝혀졌다. 이상의 결과로 우리나라 재래닭은 공통선조가 다른 5개 이상의 모계가 중국을 통하여 유래되었으며, 일본에도 전파된 것이 확인되었다. 한편, 일본은 한반도를 유래하지 않은 닭의 유입이 있었던 것으로 추정된다. In this study, we analyzed the mitochondrial DNA D-loop region of Korean native chicken to clarify their phylogenetic relationships, possible maternal origin and routes of introduction into Korea. A 1231-1232 bp DNA fragment from the mtDNA D-loop region was sequenced in 315 chickens from 11 populations, Thirty-five variable sites that defined 21 haplotyes were observed. In Korean native chicken, diversity accounted for 90% of the variation, little differentiation among the strains. The 21 haplotypes clustered into 5 clades which were A, B, C, D and E. These results indicate that Korean chickens were derived from China with multiple origins.

      • KCI등재

        호남지역 콩 포장에서 톱다리개미허리노린재(Riptortus clavatus)의 발생소장, 콩 피해 및 약제방제 효과

        이건휘,백채훈,최만영,오영진,김두호,나승용,Lee Geon-Hwi,Paik Chae-Hoon,Choi Man-Young,Oh Young-Jin,Kim Doo-Ho,Na Seung-Yeoung 한국응용곤충학회 2004 한국응용곤충학회지 Vol.43 No.3

        호남지역 콩 포장에서 톱다리개미허리노린재의 발생소장, 콩 피해 및 약제방제 효과를 검토하였다. 톱다리개미허리노린재의 발생은 콩 파종시기 및 연차간에 차이가 있었다. 톱다리개미허리노린재의 발생최성기는 5월 하순경 파종은 8월 하순에, 6월 중순경 파종은 9월 중순 이었고, 파종기가 동일하더라도 연차간 기상조건에 따른 중 생육상의 차이로 톱다리개미허리노린재의 발생최성기 차이가 있었다. 톱다리개미허리노린재에 의한 콩 흡즙 피해 면적을이 증가하면 발아율이 현저하게 낮아졌으며, 특히, 콩 피해면적율이 $50\%$ 이상 되면 발아율이 $2\%$로 매우 낮았다. 톱다리개미허리노린재에 대한 약제방제효과는 콩 생육단계중 $R_4$(착협성기)+ $R_5$(립비대기)+ $R_6$(립비대성기)에 3회 처리시 $91\%$의 방제효과로 가장 높았다. This study was conducted to determine Seasonal Occurrence, soybean damage and control efficacy of bean bug, Riptortus clavatus Thunberg at soybean field in Honam province. Occurrence of R. clavatus varied at seeding times with annual difference. The peak occurrence of R. clavatus was appeared at late of August for late May seeding and middle of September for middle June seeding. There was difference in the peak occurrence of R. clavatus due to different growing stages affected by different weather conditions though soybean was seeded at same time. If the rate of soybean seed damage area by R. clavatus sucking increases, the germinating rate was greatly decreased. If the rate of soybean seed damage area is above 50 percent, in particular, the germinating rate was very low with 2 percent. Control efficacy against R. clavatus was the highest with $91\%$ at 3 times treatment of insecticide at $R_4$ (full pod stage) + $R_5$ (Beginning seed stage) +$R_6$ (full seed stage) of soybean growing stages in field.

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