비소 세포형 폐암에서의 p53과 bcl-2 유전자의 발현에 관한 연구

명나혜,서필원,이민철 단국대학교 1998 論文集 Vol.32 No.-

We investigated immunohistochemical expression of bcl-2 and p53 genes in 30 resected human non-small cell lung cancers. We attempted to know how frequently these gene alterations occur and the correlation between their immunoreactivity and clinicopathologic parameters such as sex, histologic type, differentiation, and stage. Also, the p53 immunoreactivity was compared with that of bcl-2 to detect their inverse or positive relationship. By ABC(avidin biotin complex) immunostainings using p53 and bcl-2 primary monoclonal antibodies on the paraffin blocks, p53 gene mutation and bcl-2 overexpression were found in 29 (96.6%) and 22(73.3%) 30 cases, respectively. Both immunopositivities were detected in 21 cases(70%), whereas neither immunopositivity in only one (3.3%) case. Almost all clinicopathologic parameters revealed no significant correlation with both immunoreactivities of p53 and bcl-2 genes except for the significantly higher overepression of bcl-2 gene in the squamous cell type than that of the non-squamous cell type ant there was neither inverse nor positive relationship between p53 and bcl-2 immuno-expressions. In conclusion, most cases of the non-small cell lung cancers are found to undergo both p53 mutation and bcl-2 overexpression, but their expressivity does not have any relationship with most of clinicopathologic variables. The two genetic alterations seem to be involved coexistently in the molecular pathogenesis of the non-small cell lung cancers.

자궁경부암 선별검진을 위한 PapSure 검사에서 Spiral Brush의 유용성에 관한 연구

송언호,김어진,명나혜,박충학 대한부인종양 콜포스코피학회 2002 Journal of Gynecologic Oncology Vol.13 No.4

2001년 12월 1일부터 2002년 6월 30일까지 만 7개월 간 단국대학교의료원 산부인과 부인종양 Clinic에서 자궁경부암 선별검진을 위한 PapSure 검사를 시행한 환자 중 SpiraBrush Cx_® 검사를 실시한 33예를 대상으로 하여, 기존의 Papanicolaou 세포진 검사의 진단과 SPiraBrush Cx_®)에 의한 Papanicolaou 세포진 검사의 진단 및 최종적인 병리조직학적 진단을 비교, 분석하여 SpiraBrush Cx_®에 의한 세포진 검사가 생검에 의한 조직검사를 대체할만한 유용성이 있는지 그 여부를 조사하기 위하여 본 연구를 시행하여 다음과 같은 결과를 얻었다. 1. 환자의 연령별 분포는 21세에서 70세까지였으며 40-49세 군이 42%로 가장 많은 분포를 보였고, 평균 연령은 45.7세였다. 2. 환자의 평균 임신횟수는 3.6회였으며, 평균 출산횟수는 2.0회였다. 3. 기존의 자궁경부 세포진 검사(Conventional Papanicolaou cytology)상 진단이 음성으로 나온 경우가 19예(57.6%)였고, ASC를 포함하여 양성으로 나온 경우가 14예(42.4%)였다. 자궁경부 형광경진 검사(Speculoscopy)에서는 음성이 13예(39.4%)였고, Suspicious 진단을 포함하여 양성으로 나온 경우가 20예(60.6%)였다. SpiraBrush Cx®에 의한 세포진 검사(SpiraBrush Cx_® Papanicolaou cytology)에서는 진단이 음성인 경우가 8예(24.2%)였고, ASC를 포함하여 양성으로 나온 경우가 25예(75.8%)였다. 4. 병리조직학적 검사의 진단은 음성 12예(52.2%), 저등급 편평상피내병변(Low-grade Squamous Intraepithelial Lesion: 이하 LSIL이라 함) 2예(8.7%), 고등급 편평상피내병변(High-grade Squamous Intraepithelial lesoin: 이하 HSIL이라 함) 3예(13.0%), 편평세포암(Squamous Cell Carcinoma: 이하 SCC라 함) 6예(26.1%)였다. 5. 검사 결과의 양성판정 기준을 세포진 검사의 경우는 ASC 이상으로 하고 조직학적 검사의 경우는 LSIL이상으로 정할 경우, 기존의 Papanicolaou 세포진 검사의 민감도는 63.6%이고 특이도는 66.7%였으며, SpiraBrush Cx_®세포진 검사의 민감도는 90.9%이고 특이도는 33.3%였다. 이상의 결과로 미루어 볼 때 연구 대상의 숫자가 적어서 통계학적인 유의성은 찾을 수 없으나 SpiraBrush Cx_®가 자궁경부암 선별검진에서 생검에 의한 자궁경부의 조직학적 진단을 대체하지는 않는다고 판단된다. 그러나 기존의 Papanicolaou 세포진 검사의 민감도가 63.6%인데 반하여 SpiraBrush Cx_® 세포진 검사의 민감도는 90.9%로 훨씬 더 높았으며 이로 인해 SpiraBrush Cx_® 세포진 검사의 특이도는 33.3%로 훨씬 낮았는데 이는 매우 흥미있는 사실로서 향후 많은 Data로 더욱 연구하여 진위를 구분함이 필요하리라 사료된다. 또한 본 연구에서는 SpiraBrush Cx_® 기구의 강모들이 단단하고 날카로워서 기저막층까지의 조직과 상피층 세포들을 탈락시키면서 많은 출혈을 초래하여 슬라이드 판독에 어려움을 주었고 이는 연구 결과에 지대한 영향을 미쳤으리라고 생각되는 바, 이러한 출혈로 인한 요인을 최대한 배제하기 위하며 향후 SpiraBrush Cx_® 세포를 채취한 후 최신의 자궁경부 세포진 검사법인 ThinPrep Pap Test를 이용하거나 조직표본을 만들기 위한 Cellblock 기법을 사용하여야 한다고 판단된다. Objective : The purpose of this study was to investigate the clinical efficacy of a new stiff bristled spiral shaped brush (SpiraBrush Cx_(??))∼in) PapSure Test for cervical cancer screening, by comparing SpiraBrush Cx_(??) cytologic diagnoses with conventional Papanicolaou cytologic diagnoses and final histologic diagnoses. Methods : This study was performed in Gynecologic Oncology Clinic of Department of Obstetrics and Gynecology at Dankook University Medical Center from December I, 2001 to June 30, 2002. Thirty three cases who underwent PapSure Test (Pap cytology t Speculoscopy) were analyzed. SpiraBrush Cx_(??) Papanicolaou cytologic diagnoses were compared with conventional Papanicolaou cytologic diagnoses and histologic diagnoses as a reference standard. Results : Followings are the results summarized. 1. Age distribution was between 21 and 70. The mean age was 45.7 years. 2. The mean gravidity was 3.6 and the mean parity was 2.0. 3. There were 19 (57.6%) conventional Papanicolaou cytologic diagnoses of Negative, and 14 (42.4%) of Positive including ASC. There were 13 (39.4%) Speculoscopy diagnoses of Negative, and 20 (60.6%) of Positive including Suspicious. There were 8 (24.2%) SpiraBrush Cx_(??) cytologic diagnoses of Negative and 25 (75.8%) of Positive including ASC. 4. There were 12 (52.2%) histologic diagnoses of Negative, 2 (8.7%) of LSIL, 3 (13.0%) of HSIL, and 6 (26.1%) of SCC, according to the criteria of TBS, 2001. 5. For detecting LSIL or worse lesions, conventional Papanicolaou cytologic test yielded sensitivity of 63.6% and specificity of 66.7%. On the other hand, SpiraBrush Cx_(??) cytologic test yielded sensitivity of 90.9% and specificity of 33.3%. Conclusion : SpiraBrush Cx_(??) cytologic diagnosis does not seem to have clinical efficacy replacing the histologic diagnosis by punch biopsy in cervical cancer screening in this study. But further studies using ThinPrep Pap Test or Cellblock techniques with large data will be required and anticipated.

Touch-print Cytology of Brain Tumors : It's correlation with histololgical features

Myong, Na Hye,Park, In Ae,Chi, Je G. Seoul National University 1993 The seoul journal of medicine Vol.34 No.1

Tissue Microarray Analysis of Fas and FasL Expressions in Human Non-small Cell Lung Carcinomas;with Reference to the p53 and bcl-2 Overexpressions

Na-Hye Myong 대한의학회 2005 Journal of Korean medical science Vol.20 No.5

Trichoblastic Fibroma : A Case Report 1례 보고

Myong, Na Hye,Kwon, Soo In,Song, Kye Yong,Chi, Je G. 世宗醫學硏究所 1993 世宗醫學 Vol.10 No.1

Correlation of Histopathologic Classification with Proliferative Activity and DNA Ploidy in 120 Intracranial Meningiomas, with Special Reference to Atypical Meningioma

Na Hye Myong Korean Academy of Medical Science 1997 Journal of Korean medical science Vol.12 No.3

Reduced Expression of E-cadherin in Human Non-small Cell Lung Carcinoma

Na-Hye Myong 대한암학회 2004 Cancer Research and Treatment Vol.36 No.1

PURPOSE: E-cadherin, a calcium-dependent cell to cell adhesion molecule, plays a key role in the maintenance of tissue integrity. Reduction or loss of E-cadherin has been reported to have a role in the development of human malignancies. The expression of E-cadherin was analyzed in human non-small cell lung carcinoma (NSCLC) to elucidate the role in pulmonary carcinogenesis and determine the relationship with several clinicopathological factors and the prognosis. MATERIALS AND METHODS: Sixty five human cases of NSCLC were evaluated by immunohistochemical analysis for the expression of E-cadherin. The immunostaining results for E-cadherin were semiquantitatively interpreted, as preserved and reduced, in the tumor tissues. The E-cadherin expression was analyzed in relation to several clinicopathological data and the survival. The cell proliferation index of the tumors was evaluated by immunostaining with the Ki-67 antigen. RESULTS: Reduced E-cadherin expression was found in 51 cases of NSCLC tissues (78.4%) compared to that in the normal controls. Reduced E-cadherin expression was significantly correlated with male smokers and squamous cell type of the cancer, but not with histological grade, TNM stage and survival. The E-cadherin expression showed a weak inverse relationship with the proliferative activity of tumor cells, which was measured using the Ki-67 antigen. CONCLUSION: Our data support the hypothesis that reduced E-cadherin expression may play a role in the pathogenesis of human NSCLC, which might be associated with the control for cell proliferation. (Cancer Res Treat. 2004;36:56-61)

Subcritical water extraction (SWE) of anthocyanin from blueberries (Vaccinium corymbosum)

Hye-Ji Kang,Seon-Woo Kim,Ha-Un Yoo,Na-Yeon Kim,So-Yoon Yee,Min-Jung Ko,Myong-Soo Chung 한국산업식품공학회 2017 학술대회 및 심포지엄 Vol.2017 No.11

Subcritical water extraction (SWE) is an eco-friendly new extraction technology because it does not contain harmful organic solvent and has high extraction efficiency in a short time compared with conventional extraction methods. Blueberries (Vaccinium corymbosum) are widely known as superfood due to rich source of anthocyanin (malvidin-3-o-galgctoside) and antioxidant activity. In this study, optimal extraction condition of SWE from blueberries was determined and compared with the conventional extraction methods. SWE was carried out using a Dionex Accelerated Solvent Extractor (ASE, Model 350) under conditions of temperatures (110, 130, 150 and 170°C) and times (1, 3, 5 and 10 min). Total anthocyanin of SWE extracts was compared with hot water (60°C, 1 h) extract and pressed juice extract. The total anthocyanin content was determined by pH differential method. Considering both the extraction time and temperature conditions, the highest content of total anthocyanin content was 0.455 mg/g FW Vaccinium corymbosum at 130°C for 3 min. At high temperature and long extraction time, the anthocyanin in the blueberries will undergo thermal degradation due to low stability of anthocyanin at extreme condition. Besides, maximum yield of anthocyanin from blueberries using SWE was about 1.2 and 3.8 times more higher than hot water extract and pressed juice extract, respectively. Therefore, SWE is faster and more efficient method to extract anthocyanin from blueberries than conventional extraction methods. This study shows a possibility of SWE applied to food processing industry.

세포성 청색모반 1예

명나혜,지제근,조병규,송계용 대한병리학회 1993 Journal of Pathology and Translational Medicine Vol.27 No.5

방사선 조사후 병발된 자궁의 악성 혼합 중배엽 종양 1예

명나혜,조경자,박상윤,장자준 대한병리학회 1990 Journal of Pathology and Translational Medicine Vol.24 No.2