http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Protective effects of a novel herbal decoction on focal cerebral ischemia in a rodent model
Kim, Myung-Gyou,Choi, Jae-Hwan,Lim, Jong-Pil,Kim, Dae-Keun,Shin, Tae-Yong,Boo, Yungmin,Kim, Sun-Yeou,Kim, Hocheol,Ha, Eunyoung,Park, Hun-Kuk,Kim, Jeongseon,Lim, Ha-Sup,Kim, Ee-Hwa,Kim, Jeung-Beum,Leem Informa UK (TaylorFrancis) 2007 Neurological research Vol.29 No.suppl1
Kim, Hyeong Jun,Cho, Yeong Do,Leem, Kang-Hyun,Lee, Dong-Nyung,Kim, Ee-Hwa,Kim, Myung-Gyou,Kim, Dae-Keun,Shin, Tae-Yong,Boo, Yungmin,Lee, Je-Hyun,Kim, Hye Kyung Heyden & Son 2006 Phytotherapy research Vol.20 No.9
<P>Ephedrae Herba (EH) is obtained by drying the stems of Ephedra sinica Stapf (family Ephedraceae). EH has been used clinically to treat colds and to reduce edema in skin. The effects of EH on melanogenesis were studied in B16 murine melanocytes. The tyrosinase activity and melanin content were measured after incubation with EH. Both reverse transcription-polymerase chain reaction and cDNA microarray analysis were used to study the mechanism of EH action. EH decreased tyrosinase activity and melanin content in a dose-dependent manner. EH decreased microphthalmia-associated transcription factor (MITF) and tyrosinase gene expression. These effects were controlled by MITF-mediated regulation of tyrosinase gene expression. EH also altered the expression of about 100 other genes. These results suggest that EH may be used clinically to treat freckles and liver spots. Copyright © 2006 John Wiley & Sons, Ltd.</P>
Kim, Myung-Gyou,Leem, Kang-Hyun The Korea Association of Herbology 2014 大韓本草學會誌 Vol.29 No.1
Objectives : Polygoni Multiflori Radix (PMR), the roots of Polygonum multiflorum Thunberg, is used to nourish the blood and yin and used for preventing premature greying of the hair. There are some articles on its preventing effects on the melanogenesis. However, there is no report about its effects on the collagen and elastin. The present study was designed to investigate its effects on collagen metabolism and elastase activity. Methods : The effects of PMR on type I procollagen production and collagenase activity in human normal fibroblasts Hs68 after UVB (312 nm) irradiation were measured by ELISA method. Cells were pretreated with the PMR for 24 hours prior to UVB irradiation. After UVB irradiation, cells were retreated with the sample and incubated for additional 24 hours. The amount of collagen type I was measured with a procollagen type I C-peptide assay kit. The activity of collagenase was measured with a MMP-1 human biotrak ELISA system. The elastase activities after treatment of PMR were measured as well. Results : In the present study, the collagen production was not increased. However, the increased collagenase activity after UVB damage was significantly recovered to $50.2{\pm}14.5%$, $8.2{\pm}3.1%$, and $10.0{\pm}3.3%$ (10, 30, and $100{\mu}g/ml$). The elastase activities (10, 100, and $1000{\mu}g/ml$) significantly reduced to $75.2{\pm}5.2%$, $40.3{\pm}1.2%$, and $27.0{\pm}1.9%$, respectively. Conclusion : PMR showed the inhibitory effects on collagenase and elastase activity. These results suggest that PMR may have potential as an anti-aging ingredient in cosmetic herbal treatment.
Anti-inflammatory Effect of Arbitrary Waveform Generator Treatment in Rats
Myung-Gyou Kim,Se-Na Lee,Ilbok Seo,Kanghyun Leem,Kee Sun Ham,Hye Kyung Kim 대한의생명과학회 2008 Biomedical Science Letters Vol.14 No.1
Inflammation is the complex biological response of injured tissues to harmful stimuli. A cascade of biochemical events propagates and matures the inflammatory response, involving the local vascular system, the immune system, and various cells within the injured tissue. The immune system is often involved with inflammatory disorders, demonstrated in both allergic reactions and some myopathies, with many immune system disorders resulting in abnormal inflammation. An Arbitrary Waveform Generator (AWG) is a piece of electronic test equipment used to generate electrical waveforms for the treatment of patients. The patients with gastritis and arthritis have been known to have a relatively favorable prognosis with AWG treatment. Accordingly, we examined the effects of AWG treatment in gastritis and arthritis animal model. The compound 48/80 was used to induce animal gastritis model. The tissue malone dialdehyde (MDA) and serum histamine levels, and the activity of superoxide dismutase (SOD) in stomach tissue were measured. The tissue MDA and serum histamine levels in AWG treated groups exhibited the decreased tendency compared with control group, whereas the tissue SOD activity was slightly increased. The Freund's complete adjuvant was used to induce animal arthritis model as well. The paw edema volume and the width of ankle joint were determined. The AWG treatment significantly decreased the paw edema volume after 5th day of treatment. Although further studies should be performed to confirm the effects of AWG treatment, present study suggest that AWG treatment might be used as a complementary treatment for the gastritis or arthritis treatment.
( Myung Gyou Kim ),( Kang Hyun Leem ) 대한본초학회 2014 大韓本草學會誌 Vol.29 No.1
Objectives : Polygoni Multiflori Radix (PMR), the roots of Polygonum multiflorum Thunberg, is used to nourish the blood and yin and used for preventing premature greying of the hair. There are some articles on its preventing effects on the melanogenesis. However, there is no report about its effects on the collagen and elastin. The present study was designed to investigate its effects on collagen metabolism and elastase activity. Methods : The effects of PMR on type I procollagen production and collagenase activity in human normal fibroblasts Hs68 after UVB (312 nm) irradiation were measured by ELISA method. Cells were pretreated with the PMR for 24 hours prior to UVB irradiation. After UVB irradiation, cells were retreated with the sample and incubated for additional 24 hours. The amount of collagen type I was measured with a procollagen type I Cpeptide assay kit. The activity of collagenase was measured with a MMP-1 human biotrak ELISA system. The elastase activities after treatment of PMR were measured as well. Results : In the present study, the collagen production was not increased. However, the increased collagenase activity after UVB damage was significantly recovered to 50.2 ± 14.5%, 8.2 ± 3.1%, and 10.0 ± 3.3% (10, 30, and 100 μg/ml). The elastase activities (10, 100, and 1000 μg/ml) significantly reduced to 75.2 ± 5.2%, 40.3 ± 1.2%, and 27.0 ± 1.9%, respectively. Conclusion : PMR showed the inhibitory effects on collagenase and elastase activity. These results suggest that PMR may have potential as an anti-aging ingredient in cosmetic herbal treatment.
저칼슘 식이로 유발한 성장 저하 흰쥐에 대한 칼슘, 비타민 D 및 난황 펩타이드의 투여가 장골 길이 성장에 미치는 영향
김명규 ( Myung Gyou Kim ),김혜경 ( Hye Kyung Kim ),임강현 ( Kang Hyun Leem ) 대한본초학회 2014 大韓本草學會誌 Vol.29 No.5
Objectives : Egg yolk is composed of various important chemical substances for human health. A calcium shortage causes the growth retardation on the body growth. In this study, we examined the therapeutic effects of calcium, vitamin D and egg yolk peptide (EYP) treatment on the retardation of the longitudinal bone growth induced by low-calcium diet in adolescent rats. Methods : Low calcium diets were administrated for 15 days. During the last five days, calcium and/or vitamin D and/or EYP were administrated. The body weights, longitudinal bone growth rates, the heights of growth plates, and bone morphogenetic protein (BMP)-2 and insulin-like growth factor (IGF)-1 expressions were measured using histochemical analysis. Results : Low calcium diets caused the significant reduction in body weight gains and the longitudinal bone growth. The heights of growth plates and the expressions of BMP-2 and IGF-1 showed the impairment of body growth as well. Calcium and/or vitamin D administration could not significantly increase the longitudinal bone growth. However, calcium, vitamin D, and EYP administration significantly increased the bone growth, the growth plate height, and BMP-2 and IGF-1 expressions. Conclusions : These results suggest that EYP enhances the longitudinal bone growth in the calcium and/or vitamin D deficiency and it could be a promising agent for the treatment of children suffering from malnutrition.
우슬(牛膝) 등 복합 추출물의 monosodium iodoacetate로 유발한 흰쥐 골관절염에 대한 효과
김명규 ( Myung-gyou Kim ),서일복 ( Il-bok Seo ),임강현 ( Kang-hyun Leem ),정태진 ( Taejin Jeong ),김진석 ( Jinseok Kim ) 대한본초학회 2017 大韓本草學會誌 Vol.32 No.1
Objectives : The present study was designed to determine the effects of mixture of Achyranthis Japonicae Radix, Scutellariae Radix, and Acanthopanacis Cortex on monosodium iodoacetate (MIA)-induced osteoarthritis in rats. The mixture was composed of Achyranthis Japonicae Radix, Scutellariae Radix, and Acanthopanacis Cortex extracts. Methods : Arthritis was induced by injection of MIA into knee joints of rats. At the end of experiment, gross examination on the articular structures of knee joints were performed. Proteoglycan (PG) contents in articular cartilages were analysed as well. Tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) contents in synovial fluids were measured by ELISA method and matrix metalloproteinase 2 (MMP2), MMP9, and tissue inhibitors of metalloproteinase 1 (TIMP1) mRNA were measured by a realtime PCR. Results : The surfaces of the articular cartilage were observed. The severity of osteoarthritis in the treated group were alleviated compared with control group. PG contents in articular cartilages of the treated group were increased compared with control group. IL-1β contents in synovial fluids of the treated group were significantly decreased compared with control group. MMP2 and MMP9 mRNA contents in articular cartilages were significantly decreased compared with control group and TIMP1 mRNA contents were increased compared with control group. Conclusions : On the basis of these results, we concluded that Achyranthis Japonicae Radix-containing mixture treatment has anti-arthritic effects on the MIA-induced osteoarthritis in rats. And the effects were related with the reduction of IL-1β in synovial membranes and the consequent reduction of MMP2 and MMP9 expressions.
Jeongseon Kim,Mi-Hyeon Jang,김창주,Ee-Hwa Kim,Myung-Gyou Kim,Kang-Hyun Leem 한국식품영양과학회 2006 Journal of medicinal food Vol.9 No.2
The roots of Platycodon grandiflorum, which belongs to the Campanulaceae family, have been used as a foodmaterial and as a traditional Oriental medicine. The effect of P. grandiflorumagainst lipopolysaccharide (LPS)-stimulated in-flammation was investigated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, reverse transcription-polymerase chain reaction, prostaglandin E2 (PGE2) immunoassay, nitric oxide (NO) detection assay, and interleurkin-8 (IL-8) immunoassay on BV2 microglial cells. The aqueous extract of P. grandiflorumwas shown to suppress PGE2 synthesis andNO production by inhibiting LPS-stimulated cyclooxygenase (COX)-2 activity and expression of inducible NO synthase(iNOS) mRNAs. In addition, the treatment with P. grandiflorumreduced the LPS-induced IL-8 release. These results suggestthat P. grandifloruminhibits PGE2 and NO production through its suppression of LPS-induced COX-2 and iNOS expression,and also reduces IL-8 secretion by microglial cells.
Inhibitory effects of Angelicae Gigantis Radix on osteoclast formation
Kil, Jong-Seob,Kim, Myung-Gyou,Choi, Hye Mi,Lim, Jong-Pil,Boo, Yungmin,Kim, Ee-Hwa,Kim, Jeung-Beum,Kim, Hye Kyung,Leem, Kang-Hyun John Wiley Sons, Ltd. 2008 Phytotherapy research Vol.22 No.4
<P>Angelicae Gigantis Radix (AGR) is one of the most widely used herbal medications. AGR is the dried root of Angelica gigas Nakai (Umbelliferae), which is known as Korean angelica. This study investigated the effects of AGR on osteoclast formation using primary bone marrow cells. TNF-&agr; treatment increased tartrate-resistant acid phosphatase (Trap) positive cells and Trap activity in bone marrow cells. However, AGR significantly decreased both TNF-&agr;-induced Trap positive cells and Trap activity. RT-PCR analyses revealed that AGR decreased mRNA levels of Trap and matrix metalloproteinase-9 in TNF-&agr;-treated bone marrow cells. In addition, AGR decreased TNF-&agr;-induced activation of NF-&kgr;B. These results suggest that AGR has an inhibitory effect on the formation of osteoclasts and its effect is partially related to the NF-&kgr;B pathway. Copyright © 2008 John Wiley & Sons, Ltd.</P>