복귀돌연변이시험을 이용한 이온화에너지 처리 우유 및 아이스크림의 유전독성학적 평가

유지숙, 한선경, 신명수, 구본철, 최지현, 이주운, 조철훈, 함준상, 이완규 충북대학교 동물의학연구소 2008 Journal of Biomedical and Translational Research Vol.9 No.1

본문참고

노후 생활 적응 연구에 대한 고찰 : 홀로된 여성 노인 중심

이명숙 안성산업대학교 1993 論文集 Vol.25 No.-

This study attempts to analyze the life adjustment of the older window. The influenced varibles for the older widow's life adjustment had been found out through the review of literature. The results are summarized as follows; (1) During the 1980's, the following topics about the life adjustment of the aged were addressed;life satisfaction, well-being, maladjustment, stress, loneliness, discrimination. (2) The variables which influenced the life adjustment of the aged were age, health, education, self-esteem, sex role-identity, perceived controllability, family relationship, marital status, economic well-being, social activities. (3) The older widow tend to experience low life satisfaction levels. The varibles which influenced the life adjustment of the older widow were economic status, health, loneliness, social activities.

Pseudomonas putida 378이 생산하는 Lipoprotein lipase의 성질

정명주,박형숙 慶星大學校 環境問題硏究所 1993 環境硏報 Vol.3 No.1

Pseudomonas putida 378이 생산하는 LPL을 정제하여 이에 대한 몇 가지의 생물화학적 성질을 검토하여 다음과 같은 결과를 얻었다. 1. 배양액으로부터 ethanol 분획, Ammonium Sulfate 침전, DEAE-Cellulose column chromatography, CM-Sephadex column chromatography, Gel filtration를 거쳐 LPL을 정제하였다. 이때 효소는 122배 정제되었고, 회수율은 11.77%였다. 2. 정제된 효소는 SDS-PAGE에서 단일 band를 보여주어, 순수하게 정제되었음을 나타내었고, 분자량은 Gel filtration법에 의해 약 108,000으로 나타났고, SDS-PAGE에 의하여 약 107,000으로 나타났으므로, 본 효소액은 monomer로 생각된다. 3. 효소의 최적 pH는 7.0이며, 최적온도는 45℃이었으며, pH는 5.0~8.0 범위에서 안정하였고, 온도는 60℃까지 안정하였다. 4. 본 효소는 1mM Ca 에 의해 활성화 되며, 10mM Hg , Ni 에 의해 저해되었다. 5. 본 효소의 각종 지질에 대한 가수 분해율은 대조구인 olive oil에 비해 lecithin에 대해 다소 높았으며, stearic aid와 tributyrin에 대해서는 매우 낮았다. The purification and properties of the lipoprotein lipase from Pseudomonas putida 378 were investigated. It was purified by ethanol, ammonium sulfate fractionation and chromatographies on DEAE- Cellulose, CM-Sephadex and gel filtration of Sephadex G-100. The enzyme was purified about 122 fold with a yield of 11.77%. The purified enzyme showed a single protein band on SDS-PAGE. Its molecular weight was estimated to be about 108,000 and 107,000 by gel filtration and SDS-PAGE, respectively. Lipoprotein lipase had an optimum pH of 7.0, and an optimal temperature of 45℃, and the stable range of pH was 5.0 to 8.0. The enzyme was thermostable at below 60℃. The enzyme was strongly inhibited by Hg (10mM), Ni (10mM) and showed the most activity ohn Ca (1mM). This lipoprotein lipase was capable of hydrolyzing a variety of oils, showing were active on lecithin than on olive oil.

흰쥐 중추신경계내 난소로 투사하는 미주신경로에 관한 연구

김명주,장명세,고미희,노해숙,조해영,오문유,이봉희 濟州大學校 基礎科學硏究所 2001 基礎科學硏究 Vol.14 No.1

본 실험은 부교감신경의 하나인 미주신경이 난소를 지배하는 신경으로 관여하고 있는지를 pseudorabies 바이러스를 이용하여 난소신경로와 난소주사후 미주신경절단을 통하여 조사한 연구 보고이다. 이를 위하여 Sprague Dawley계 암흰쥐를 대상으로 pseudorabies 바이러스를 난소에 주사한 무리와 난소 주사후 미주신경을 절단한 무리에서 뇌를 적출하여 pseudorabies 바이러스에 대한 면역조직화학 염색을 시행하여 비교하였다. 본 실험결과 미주신경 중추신경로내의 상위신경핵들이 pseudorabied 바이러스에 대하여 양성반응이 줄어들었거나 관찰되지 않는 차이를 보였다. 즉 적색핵, 종말판혈관기관, 줄무늬체, 침상핵과 이마엽겉질은 부분적으로 난소의 미주신경로에 관여하고 있으며 미주신경등쪽핵, 고립로핵, 최후영역, 청색반점, 팔옆핵, 코리케퓨즈핵, 흑색질 및 시각교차위핵은 양성반응이 관찰되지 않아 미주신경으로 투사하는 부교감신경핵으로 조사되었다. The mammalian ovary has been known as receiving its innervation by sympathetic and sensory neurons of the peripheral nervous system from the brain. Recently, there were several functional reports that the vagus nerves were also regulating the ovarian function, but the vagus nerve had not been identified by clear morphological evidence. A viral transneuronal tracing technique has been used to demonstrate the morphological evidence for the central vagal involvement in ovarian innervation in brain areas. Bartha strain of pseudorabies virus injection was made into the ovary of Sprague Dawley rats. In experimental group, the vagus nerve of the same injection side was removed right after ovarian injection. At five days after initial injection, all the rats were sacrificed and brains were processed for immunohistochemistry. Several central nuclei including hypothalamic paraventricular nucleus showed strong bilateral positive labelings after unilateral injection in control rats, but the positive labelings were disappeared or decreased in several hypothalamic nuclei and nuclei of the vagus nerve. I n conclusion, these results provide the morphological evidence that vagus nerve has neural connection to ovary and by which the central nervous system may maintains the state of ovulation and reproduction as a possible parasympathetic routes in mammals.

1920년대 신여성 등장 배경

김명숙 동덕여자대학교 학생생활연구소 2006 젠더연구 Vol.- No.11

Modern women who appeared in United Kingdom in 1890's became social phenomenon and cultural token all over the world in 1920's. Women in 1920's, which is perceived to be typical era for outlet of modern spirit, called themselves 'modern women' insisting extension of general job training, economic independence, and equality between male and female so that the time was awakening era for women to be reconsider their images. They have changed all the value, code, system, and cultural aspect surrounding traditional manner of sex, marriage, and family in a great deal. It was perceived to be understood as challenge towards modern society and male-centered world. Modern women who showed up in Korea in 1920's were not the exceptional case. However, considering the fact that Chosun was under the rein of Japanese at that time, they have characteristics of combination between Japanese modern women in 1910's and that of 1920's. They as progressive and liberal actively supported and practiced modern feminism such as the principle of the emancipation of women, free love, and freedom of sexual love came in Korea in 1920's ~ 30's by western feminism thinkers such as Ibsen, Bevel, Ellen Key, and Kollontay who affected women's liberation movement. Chosun as a colony thought of modern place as western world (or western perceived through Japan). Sometimes Japan was understood as the equal terminology of modern world. The new life-style they pursued was in fact pursuit of Americanism. The term, Americanism combined by technology, equality, efficiency, social mobility, usefulness, physicality, and collectivity was what modern women who were the token of modern world wanted to enjoy.

P3-F계 세제의 살균력 측정

정명주,손태문,박형숙 慶星大學校 1998 論文集 Vol.19 No.2

The microbial activity of P3-luboklar FC,FC-A,FC-A(XT)DX and DXB was compared by DGHM Germ Carrier Test method. The following results were obtained. 1. FC,KB and KC has shown to be effective against all strains within 10 minutes. 2. FC-A was effective against E. coli, immediately, and kill could be achieved against Pseudomonas aeruginosa and Staphylococcus aureus within 4 minutes and 16 minutes, respectively. 3. DXB was effective against all strains within 10 minutes with the exeption of B. subtilis. 4. FC-A(XT) was the most effective against B. subtilis.

魚村地域(東海岸)中學生의 도시락營養實態調査

張明淑 관동대학교 1977 關大論文集 Vol.5 No.1

This Study is concerned with the nutritional status of the packed lunch boxes which are brought by the Middle School Students in A Fishery Villages Area (East-coast Region). Seven hundred and eighty nine students had been randomly sleeted as subjects in July 1976. The contained nutrients in the packed lunch were analysed by the Food Composition Table. To observe the influence of home economical status and mother's educational level on the nutrient contents of packed lunches, the chosen subjects were classified into three large groupings, which are upper, middle and lower classes respectively. In addition, comparison between the Recommended Daily Allowances for Korean People-13 to 15 age group-and the contained nutrientsin the lunch boxes had been conducted. 1. All of the nutrients in the lunches fall far behind the Recommended Daily Allowances for 13 to 15 age group. 2. Home economical status gives influence on the kinds of foods which could been brought by the subject Students. 3. Regardless to the living conditions of the subject students, all nutrients of the lunch boxes packed by the mothers of the entire educational levels did not reach the Recommended Daily Allowances. 4. The main dish of the lunch boxes supplied Calorie, Protein, Vitamin, VitaminB( ) and Niacin which stand at 87.8%, 59.8%, 72%, 62.5% and 66.7% respectively when compared with the whole nutritional contents. 5. The higher the level of mother's educational and of the home economical status, the more consumption of meat and fish appears. 6. The side-dishes of the packed lunch lack in variety of cooking methods and further show lower utilization rate of specialty foods particular in the region.

Bisphosphonate와 Indomethacin이 백서 치조골의 골개조에 미치는 영향

조명숙,김종철 대한치과교정학회 1996 대한치과교정학회지 Vol.26 No.2

본 실험은 서로 다른 기전으로 골흡수를 억제한다고 추정되는 약제인 bisphosphonate와 indomethacin을 백서에 투여한 후 교정력을 이용한 치아이동시 약제가 골개조에 미치는 영향을 밝히고자 시행되었다. 동일한 조건에서 사육된 체중 260-300g의 웅성백서(Sprague-DawleyrP)를 대조군, bisphosphonate 투여군 및 indomethacin 투여군으로 분류하고 각 군은 다시 장치를 장착한 실험측과 장치를 장착하지 않은 대조측으로 분류하였다. Bisphosphonate(6.3mg/kg,2.52x10 mol/L)와 indomethacin(9mg/kg, 2.52x10 mol/L)은 교정장치 장착 6시간 전, 1시간 전 및 24시간 후에 복강내 주사하였으며, 교정력이 가해진 시점으로부터 72시간이 경과한 후 파골세포수를 측정하고 조직학적인 성상을 관찰하였다. 또한 혈액을 채취한 후 혈청 acid phosphatase ac lactate dehydrogenase 양을 측정하여 다음과 같은 성적을 얻었다. 실험측의 파골세포수는 장치장착 1시간 전에 투여한 bisphosphonaterns과 indomethacinrns에서 가장 적게 나타났으며, 다른 시간의 약물 투여군은 대조군과 차이가 없었다. 대조측의 파골세포수는 실험측보다 현저히 적게 나타났으며, 대조군 및 약물투여군간에 차이가 없었다. 대조군과 6시간 전 및 24시간 후에 투여한 indomethacin군은 심한 골흡수 양상을 보인 반면 1시간전 indomethacinrns 및 모든 bisphosphonate군에서는 골흡수의 정도가 적었다. 파골세포의 주름변연과 투명대는 대조군 및 indomethacin군에서 가장 뚜렷하게 나타났으며, bisphosphonate 투여군에서는 일부 파골세포들이 무딘 세포돌기만을 내거나 골표면에 부착하지 않고 있는 경우가 많았다. Bisphosphonate와 indomethacin 투여군 모두에서 acid phosphatase 및 lactate dehydrogenase 값이 대조군보다 낮았으며, acid phosphatase 값은 bisphosphonaterns이 indomethacinrns보다 낮았으나 lactate dehydrogenase 값은 차이가 없었다. 이상의 결과로 bisphosphonate는 파골세포의 수 및 대사활동을 감소시키며 indomethacin은 파골세포의 수를 감소시킴을 알 수 있었다. 또한 두 약물을 비교하면 bisphosphonate는 indomethacin에 비해 골흡수 억제효과가 더 크며 투여시간에 따른 제약도 더 적은 것으로 사료된다. The purpose of the study was to examine the effects of bisphosphonate and indomethacin, blockers of bone resorption with different mechanisms, on alveolar bone remodeling. Male rats were divided into control, bisphosphonate and indomethacin groups, and then each group was divided into an experimental side and a control side according to the force application. Bisphosphonate(6.3mg/kg,2.52x10 mol/L) and indomethacin (9mg/kg, 2.52x10 mol/L) were injected 6 hours and I hour before or 24 hours after the force application. The rats were killed 72 hours after the force application and histologic examination was perfomed. The values of serum acid phosphatase and lactate dehydrogenase were also measured in the control and experimental groups treated with bisphosphonate or indomethacin 1 hour before the force application. In the experimental side, the least number of osteoclasts was noted in the groups treated 1 hour before the force application with indomethacin or bisphosphonate, while there were no differences between the control and the groups treated with drugs 6 hours before or 24 hours after the force application. In the control side, the number of osteoclasts was not inecreased with no differences among the groups. Histologic examination revealed a severe alveolar bone resorption in the control group and the groups treated with indomethacin 6 hours before or 24 hours after the force application. Indomethacin treatment 1 hour before the force application and bisphosphonate treatment at any time significantly attenuated the bone resorption. Electron microscopically, ruffled border and clear zone of osteoclasts were observed in the control and indomethacin groups, while some osteoclasts were detached from the bone surface and exhibited dull cellular projections in the bisphosphonate groups. The bisphosphonate and indomethacin groups showed lower values of acid phosphatase and lactate dehydrogenase than the control group. The acid phosphatase value in the bisphosphonate group was lower than that in the indomethacin group, whereas there was no difference in the lactate dehydrogenase value between the groups. These results suggest that bisphosphonate reduces the activity of osteoclasts as well as the number of osteoclasts and that indomethacin reduces the number of osteoclasts without affecting the activity of osteoclasts. Bisphosphonate has a larger inhibitory effect on bone resorption and thus less limitation in the application time than indomethacin.

전통놀이가 유아의 사회성 발달에 미치는 효과 : 비석치기, 윷놀이, 고누놀이를 중심으로

신명숙,류점숙 한국아동교육학회 2003 아동교육 Vol.12 No.1

The purpose of this study was to provide basic materials to help korean traditional plays be effectively utilized in early children education. It was investigated that how traditional plays(Striking a stone, Playing yut, and Playing stone checkers)had an impact on the social development of 5-year-old children. The subject of study were 60 5-yesr-old children that were registered in a kindergarten as an annex to P elementary school in Y city of the northern province of Kyungsang. An experimental group of 30 and a control group of 30 were assigned. To assess the young children's social development, the measure of social development for young children by Sukkyung Kim (1989) was utilized. The program of traditional plays was implemented in an experimental group of 30 for 8 weeks, while the program was not conducted in a control group of 30. After the mean and the standard deviation of the measure in two groups before/after the program were computed, t-test was run to verify the difference between the two groups. The findings from the research were as follows: First, traditional plays had a statistically significant impact on the development of cooperation in 5-year-old children. Second, traditional plays had a statistically noteworthy influence on the development of understanding of other on 5-year-old children. Third, traditional plays had a statistically considerable effect on the development of self-control in 5-year-old children. Fourth, traditional plays effected the development of peer interaction in 5-year-old children with a statistical significance.

상악 중절치 결손을 동반한 골격성 Ⅱ급 부정교합 환자의 치험예

조명숙,김종철 대한치과교정학회 1989 대한치과교정학회지 Vol.19 No.2

The causes of the missing teeth are classified as congenital missing, trauma and extraction due to dental caries, variable problems are occured clinically by the missing teeth. The missing of the upper incisors especially would assume a serious aspect, and could be treated by three methods of orthodomtic treatment, prosthodontic treatment and autotrans-plantation of the premolar teeth. The patient of this report had the skeletal class II malocclusion with the left upper central incisor missing, and have been treated with the fixed applianc after extraction of the right upper central incisor and both lower second premolars. The result were obtained as follows: 1. Treatment was done 1 year 6 months. 2. Normal overbite and overjet were achieved. 3. Cuspal interdigitation was obtained normally. 4. Space problem was resolved with resin restoration of the upper lateral incisors. 5. The upper canfles were used as the upper laterals after cuspal contouring. 6. Retention would be required with adequate retainers for a long time to prevent relapsing after treatment.