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Biomonitoring of Toxic Effects of Pesticides in Occupationally Exposed Individuals
Arshad, Muhammad,Siddiqa, Maryam,Rashid, Saddaf,Hashmi, Imran,Awan, Muhammad Ali,Ali, Muhammad Arif Occupational Safety and Health Research Institute 2016 Safety and health at work Vol.7 No.2
Background: Workers in pesticide manufacturing industries are constantly exposed to pesticides. Genetic biomonitoring provides an early identification of potential cancer and genetic diseases in exposed populations. The objectives of this biomonitoring study were to assess DNA damage through comet assay in blood samples collected from industry workers and compare these results with those of classical analytical techniques used for complete blood count analysis. Methods: Samples from controls (n = 20) and exposed workers (n = 38) from an industrial area in Multan, Pakistan, were subjected to various tests. Malathion residues in blood samples were measured by gas chromatography. Results: The exposed workers who were employed in the pesticide manufacturing industry for a longer period (i.e., 13-25 years) had significantly higher DNA tail length ($7.04{\mu}m$) than the controls ($0.94{\mu}m$). Workers in the exposed group also had higher white blood cell and red blood cell counts, and lower levels of mean corpuscular hemoglobin (MCH), MCH concentration, and mean corpuscular volume in comparison with normal levels for these parameters. Malathion was not detected in the control group. However, in the exposed group, 72% of whole blood samples had malathion with a mean value of 0.14 mg/L (range 0.01-0.31 mg/L). Conclusion: We found a strong correlation ($R^2=0.91$) between DNA damage in terms of tail length and malathion concentration in blood. Intensive efforts and trainings are thus required to build awareness about safety practices and to change industrial workers' attitude to prevent harmful environmental and anthropogenic effects.
Biomonitoring of Toxic Effects of Pesticides in Occupationally Exposed Individuals
Muhammad Arshad,Maryam Siddiqa,Saddaf Rashid,Imran Hashmi,Muhammad Ali Awan,Muhammad Arif Ali 한국산업안전보건공단 산업안전보건연구원 2016 Safety and health at work Vol.7 No.2
Background: Workers in pesticide manufacturing industries are constantly exposed to pesticides. Genetic biomonitoring provides an early identification of potential cancer and genetic diseases in exposed populations. The objectives of this biomonitoring study were to assess DNA damage through comet assay in blood samples collected from industry workers and compare these results with those of classical analytical techniques used for complete blood count analysis. Methods: Samples from controls (n = 20) and exposed workers (n = 38) from an industrial area in Multan, Pakistan, were subjected to various tests. Malathion residues in blood samples were measured by gas chromatography. Results: The exposed workers who were employed in the pesticide manufacturing industry for a longer period (i.e., 13-25 years) had significantly higher DNA tail length (7.04 ㎛) than the controls (0.94 ㎛). Workers in the exposed group also had higher white blood cell and red blood cell counts, and lower levels of mean corpuscular hemoglobin (MCH), MCH concentration, and mean corpuscular volume in comparison with normal levels for these parameters. Malathion was not detected in the control group. However, in the exposed group, 72% of whole blood samples had malathion with a mean value of 0.14 mg/L (range 0.01-0.31 mg/L). Conclusion: We found a strong correlation (R2 = 0.91) between DNA damage in terms of tail length and malathion concentration in blood. Intensive efforts and trainings are thus required to build awareness about safety practices and to change industrial workers’ attitude to prevent harmful environmental and anthropogenic effects.
Mariyam,Muhammad Shafiq,Muhammad Haseeb,Rana Muhammad Atif,Syed Agha Armaghan Asad Abbas Naqvi,Numan Ali,Muhammad Arshad Javed,Fizza Gillani,Muhammad Saleem Haider 한국원예학회 2021 Horticulture, Environment, and Biotechnology Vol.62 No.6
DNA binding with one fi nger (Dof) proteins are encoded by a ubiquitous plant-specifi c transcription factor gene family thatplays a critical role in various biological processes including fruit ripening and organogenesis. The wild olive ( Olea europaeavar. sylvestris v1.0 ) genome was used to identify Dof gene family members using a set of bioinformatics tools. Gene structure,chromosome locations, phylogeny, protein motifs, miRNA targets and tissue-specifi c expression patterns were analyzed. Here, we identifi ed 51 potential Dof genes unevenly distributed on all chromosomes and a few scaff olds. Dof proteins in oliveclustered into eight subgroups (D1, B2, C3, C2.2, C1, C2.1, B1, and A) based on the established Arabidopsis classifi cation. The prevalence of segmental duplication was observed as compared to tandem duplication, and this was the main factorunderlying the expansion of the Dof gene family in olive. Tissue-specifi c expression profi ling of Oeu Dof genes revealed thatthe majority of Oeu Dof genes were highly expressed in fl owers, stem and meristem tissues. In seed and meristem tissues,cis-regulatory element (CRE) analysis revealed the presence of elements that are specifi cally responsive to light, circadian,auxin, and ABA. In addition, a comparative analysis between Dof genes in olive and Arabidopsis revealed eight groups orsub-families, although the C3 group of Arabidopsis was not represented in olive. This extensive genome evaluation of theDof gene family in olive presents a reference for cloning and functional analysis of the members of this gene family.
Effects of elastic medium on buckling of microtubules due to bending and torsion
Taj, Muhammad,Hussain, Muzamal,Afsar, Muhammad A.,Safeer, Muhammad,Ahmad, Manzoor,Naeem, Muhammad N.,Badshah, Noor,Khan, Arshad,Tounsi, Abdelouahed Techno-Press 2020 Advances in concrete construction Vol.9 No.5
Microtubules buckle under bending and torsion and this property has been studied for free microtubules before using orthotropic elastic shell model. But as microtubules are embedded in other elastic filaments and it is experimentally showed that these elastic filaments affect the critical buckling moment and critical buckling torque of the microtubules. To prove that, we developed orthotropic Winkler like model and demonstrated that the critical buckling moment and critical buckling torque of the microtubules are orders of higher magnitude than those found for free microtubules. Our results show that Critical buckling moment is about 6.04 nNnm for which the corresponding curvature is about θ = 1.33 rad /㎛ for embedded MTs, and critical buckling torque is 0.9 nNnm for the angle of 1.33 rad/㎛. Our results well proved the experimental findings.
Saira Sattar,Muhammad Imran,Zarina Mushtaq,Muhammad Haseeb Ahmad,Muhammad Sajid Arshad,Melvin Holmes,Joanne Maycock,Muhammad Faisal Nisar,Muhammad Kamran Khan 한국식품과학회 2020 Food Science and Biotechnology Vol.29 No.10
The peach functional beverages pasteurized for10 min at 90 C, microwaved for 1.5 min at 850 W ofpower and sonicated for 90 min at 20 kHz of frequencywere selected to keep in storage for up to 30 days inrefrigerator to examine the changes happened to theirphysicochemical characteristics and functional components. It was observed that the pH and the cloud values ofall processed juice samples reduces with the storage time,whereas, the total soluble solids almost remain consistentparticularly in microwave and ultrasound treated samples. While storage period causes the decrement in total phenoliccontent (TPC) and total flavonoid content of treated beveragesamples, but ultrasound processing showed greaterretention of TPC value up to 5.7% more than other techniquesduring storage. The similar trend was observed forantioxidant activity where the ultrasound treatment showedimproved free radicals (2,2-diphenyl-1-picrylhydrazyl and2,20-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)scavenging activities except ferric ion reducing antioxidantpower after 30 days of storage.
Arshad, Muhammad Zeeshan,Nawaz, Javeria Muhammad,Hong, Sang Jeen Korea Information Processing Society 2014 Journal of information processing systems Vol.10 No.3
In this paper, we investigated the use of seasonal autoregressive integrated moving average (SARIMA) time series models for fault detection in semiconductor etch equipment data. The derivative dynamic time warping algorithm was employed for the synchronization of data. The models were generated using a set of data from healthy runs, and the established models were compared with the experimental runs to find the faulty runs. It has been shown that the SARIMA modeling for this data can detect faults in the etch tool data from the semiconductor industry with an accuracy of 80% and 90% using the parameter-wise error computation and the step-wise error computation, respectively. We found that SARIMA is useful to detect incipient faults in semiconductor fabrication.
Anthropogenic impact on the distribution of the birds in the tropical thorn forest, Punjab, Pakistan
Muhammad Altaf,Arshad Javid,Abdul Majid Khan,M.S.H. Khan,Muhammad Umair,Zulfiqar Ali 국립중앙과학관 2018 Journal of Asia-Pacific Biodiversity Vol.11 No.2
The present research was conducted to assess the anthropogenic impacts on avian diversity around the river Chenab, Pakistan. The avian diversity was recorded on different landscapes along the river Chenab, that is, forest, agricultural, rural, and urban habitats. The data on diversity and distribution of various avian species were collected through linear count along with direct count methods viz. personal observations and sound records and indirect count methods viz. presences of carcasses and meeting with local communities. The habitat preferences of birds varied significantly. A decline in avian diversity was observed from forest habitat to urban landscapes. One year of bird surveys compiled a total of 150 species from all habitats along the river Chenab. Computed statistical data show that Shannon's index provides a quantitative report of diversity which was highest at forest habitat (4.261) followed by rural forest habitat (3.746), agri–rural habitat (3.746), agriculture habitat (3.623), WLH (2.723), urban vegetative habitat (3.215), and very least at urban nonvegetative habitat (2.247). It can be concluded from the present study that many of the avian species have specific habitats and also noted that corridors and connections among different landscapes are important for the conservation of avian diversity.
Dengue Virus Serotypes Circulating in Khyber Pakhtunkhwa Province, Pakistan, 2013-2015
Muhammad Suleman,Rani Faryal,Muhammad Masroor Alam,Salmaan Sharif,Shahzad Shaukat,Uzma Bashir Aamir,Adnan Khurshid,Mehar Angez,Massab Umair,Mian Muhammad Sufian,Yasir Arshad,Syed Sohail Zahoor Zaidi 대한진단검사의학회 2017 Annals of Laboratory Medicine Vol.37 No.2
From 2013 to 2015, the National Institute of Health, Pakistan, received 1,270 blood samples of suspected dengue cases reported from inpatient and outpatient departments of various hospitals in Khyber Pakhtunkhwa (KPK) province. In this study, we determined the circulating dengue virus (DENV) serotypes using real-time reverse transcriptase (RT)-PCR to understand the serotype-based epidemiology of DENV. All four serotypes (DENV-1 [6%], DENV-2 [33%], DENV-3 [47%], and DENV-4 [0.1%]) were found circulating during the study period. Our findings suggest the need for an active surveillance system coupled with the laboratory diagnosis, especially in the chronic endemic areas of the country. Public awareness programs are needed for effective control and prevention of outbreaks in the future.
FIXED POINTS OF CHATTERJEA TYPE MULTI-VALUED F-CONTRACTIONS ON CLOSED BALL
Muhammad Nazam,Muhammad Arshad,Aftab Hussain,H. G. Hyun 경남대학교 기초과학연구소 2018 Nonlinear Functional Analysis and Applications Vol.23 No.2
We introduce the notions of Chatterjea type multi-valued F-contraction and Chatterjea type multi-valued (α, η, GF )-contraction on closed ball and obtain two new fixed point theorems for such kind of contractions in complete metric spaces. Some comparative examples are given to illustrate and to show usefulness of these results among famous fixed point theorems on F-contractions.
Interactive Slide Navigation: An Approach for Manipulating Slides with Augmented Reality Markers
Muhammad Tahir,Muhammad Arshad Awan,Aniqa Hafeez,Mohsin Yazdanie,Sheikh Ziauddin 한국산학기술학회 2012 SmartCR Vol.2 No.2
The use of PowerPoint slides is an interactive way of communication in a classroom environment. To make it more effective, different techniques for the manipulation, annotation, and navigation of slides have been utilized. We presented the use of Augmented Reality technology to manipulate, annotate and navigate through PowerPoint slides. We used Augmented Reality marker recognition techniques by employing an Augmented Reality ToolKit. We performed tests by analyzing Augmented Reality marker IDs and their translational and rotational gestures. We used 1) simple flat Augmented Reality markers, 2) finger Augmented Reality markers, 3) cube markers, 4) illuminated markers, and 5) retro-reflective markers to manipulate slides. The results indicate that finger Augmented Reality markers are easy to handle and perform better than other types of markers. Cube markers are especially helpful in performing rotational gestures, while illuminated and retro-reflective markers provide moderate results in comparison with simple flat markers. As far as we know, we are the first to use Augmented Reality technology for handling slides and providing the presenter with distant access to the presentation.